scholarly journals Comparison of Gut Bacterial Communities of Fall Armyworm (Spodoptera frugiperda) Reared on Different Host Plants

2021 ◽  
Vol 22 (20) ◽  
pp. 11266
Author(s):  
Dongbiao Lv ◽  
Xueying Liu ◽  
Yanlu Dong ◽  
Zizheng Yan ◽  
Xuan Zhang ◽  
...  
Keyword(s):  
Microbial Community ◽  
Microbial Diversity ◽  
Oilseed Rape ◽  
Spodoptera Frugiperda ◽  
Economic Losses ◽  
Seed Coating ◽  
Wild Oat ◽  
Coating Agent ◽  
Wide Range ◽  
Gut Microbial Community

Spodoptera frugiperda is a highly polyphagous and invasive agricultural pest that can harm more than 300 plants and cause huge economic losses to crops. Symbiotic bacteria play an important role in the host biology and ecology of herbivores, and have a wide range of effects on host growth and adaptation. In this study, high-throughput sequencing technology was used to investigate the effects of different hosts (corn, wild oat, oilseed rape, pepper, and artificial diet) on gut microbial community structure and diversity. Corn is one of the most favored plants of S. frugiperda. We compared the gut microbiota on corn with and without a seed coating agent. The results showed that Firmicutes and Bacteroidetes dominated the gut microbial community. The microbial abundance on oilseed rape was the highest, the microbial diversity on wild oat was the lowest, and the microbial diversity on corn without a seed coating agent was significantly higher than that with such an agent. PCoA analysis showed that there were significant differences in the gut microbial community among different hosts. PICRUSt analysis showed that most of the functional prediction categories were related to metabolic and cellular processes. The results showed that the gut microbial community of S. frugiperda was affected not only by the host species, but also by different host treatments, which played an important role in host adaptation. It is important to deepen our understanding of the symbiotic relationships between invasive organisms and microorganisms. The study of the adaptability of host insects contributes to the development of more effective and environmentally friendly pest management strategies.

scholarly journals STRUKTUR KOMUNITAS MIKROBA TANAH DAN IMPLIKASINYA DALAM MEWUJUDKAN SISTEM PERTANIAN BERKELANJUTAN

el–Hayah ◽  
2012 ◽  
Vol 1 (4) ◽  
Author(s):  
Prihastuti Prihastuti
Keyword(s):  
Microbial Community ◽  
Microbial Diversity ◽  
Microbial Communities ◽  
Soil Microbial Community ◽  
Soil Microbial Communities ◽  
Organic Soil ◽  
Plant Type ◽  
Soil Microbial Community Structure ◽  
Soil Microbial ◽  
Wide Range

<p>Soils are made up of organic and an organic material. The organic soil component contains all the living creatures in the soil and the dead ones in various stages of decomposition.  Biological activity in soil helps to recycle nutrients, decompose organic matter making nutrient available for plant uptake, stabilize humus, and form soil particles.<br />The extent of the diversity of microbial in soil is seen to be critical to the maintenance of soil health and quality, as a wide range of microbial is involved in important soil functions.  That ecologically managed soils have a greater quantity and diversity of soil microbial. The two main drivers of soil microbial community structure, i.e., plant type and soil type, are thought to exert their function in a complex manner. The fact that in some situations the soil and in others the plant type is the key factor determining soil microbial diversity is related to their complexity of the microbial interactions in soil, including interactions between microbial and soil and microbial and plants. <br />The basic premise of organic soil stewardship is that all plant nutrients are present in the soil by maintaining a biologically active soil environment. The diversity of microbial communities has on ecological function and resilience to disturbances in soil ecosystems. Relationships are often observed between the extent of microbial diversity in soil, soil and plant quality and ecosystem sustainability. Agricultural management can be directed toward maximizing the quality of the soil microbial community in terms of disease suppression, if it is possible to shift soil microbial communities.</p><p>Keywords: structure, microbial, implication, sustainable agriculture<br /><br /></p>

scholarly journals Influence of Oilseed Rape Seed Treatment with Imidacloprid on Survival, Feeding Behavior, and Detoxifying Enzymes of Mustard Aphid, Lipaphis erysimi

Insects ◽  
2019 ◽  
Vol 10 (5) ◽  
pp. 144
Author(s):  
Fang Huang ◽  
Zhongping Hao ◽  
Fengming Yan
Keyword(s):  
Feeding Behavior ◽  
Oilseed Rape ◽  
Lipaphis Erysimi ◽  
Seed Coating ◽  
Detoxifying Enzymes ◽  
Feeding Behaviors ◽  
Mustard Aphid ◽  
Coating Agent ◽  
Aphid Feeding ◽  
Rape Seedlings

Imidacloprid application, as a seed coating agent on oilseed rape, is recommended to control mustard aphid, Lipaphis erysimi (Kaltenbach) (Hemiptera: Aphididae). In this study, responses of L. erysimi were investigated, including survival, feeding behavior, and detoxifying enzymes, on the oilseed rape seedlings grown from seeds coated with imidacloprid at rates of 6, 12, or 18 g active ingredient (a.i.)/kg seed. The results showed that the aphids’ survival rate, together with that of the progeny of the survivors, on the seed-treated seedlings significantly decreased. This indicates that the aphid population in fields can be suppressed effectively. The electrical penetration graph (EPG) technique was used to record aphid feeding behaviors on two-, four-, and six-leaf stages of oilseed rape seedlings that had been seed-coated with imidacloprid, and individual responses were revealed during the aphid feeding behavior. On the plants at the two-leaf stage, aphid feeding behaviors were influenced, showing decreased frequency of stylet penetration into the leaf (probe) or into the mesophyll cells (potential drops, pds for short), and shortened duration of stylet event in the leaf (probe) or in the phloem. On the plants at the four- and six-leaf stages, these impacts of imidacloprid were weakened; however, the saliva secretion duration in phloem was shortened to less than 5 min in all imidacloprid treatments. The activity of mixed-function oxidase in aphids maintained on the treated seedlings with imidacloprid was elevated. In conclusion, imidacloprid could be used as a seed coating agent for aphid control, but chemical resistance in aphids should not be ignored.

scholarly journals Rapid identification of the invasive fall armyworm Spodoptera frugiperda (Lepidoptera, Noctuidae) using species-specific primers in multiplex PCR

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Cheng-Lung Tsai ◽  
I.-Hsuan Chu ◽  
Ming-Hsun Chou ◽  
Theeraphap Chareonviriyaphap ◽  
Ming-Yao Chiang ◽  
...  
Keyword(s):  
Spodoptera Frugiperda ◽  
Egg Production ◽  
Mainland China ◽  
Fall Armyworm ◽  
The United States ◽  
Rapid Identification ◽  
Economic Losses ◽  
Specific Primers ◽  
Wide Range ◽  
Species Specific

Abstract The fall armyworm (FAW), Spodoptera frugiperda (Smith), is a major pest native to the Americas. A recent invasion of FAWs from Africa eastward to South Asia, the Indochina Peninsula, and mainland China has received much attention due to the considerable economic losses in agriculture. FAWs can rapidly colonise a new area, likely due to the wide range of host plants, good flying capability, and high egg production. Therefore, a convenient, quick, and accurate tool for FAW identification is urgently required to establish a FAW invasion management strategy. In this study, FAW-specific primers were designed to recognise FAWs on the basis of internal transcribed spacer 1 (ITS1). The results revealed the accurate FAW recognition of the three congeneric species and eight common corn lepidopteran pests, especially at their larval stage. Furthermore, species-specific primers have confirmed their efficacy by using 69 FAW specimens from Taiwan, Thailand, and the United States, with a 96% success rate, excluding 3 decayed specimens. By using the simple, reliable, and convenient FAW-specific primers, a pest management programme can be developed not only to reduce sequencing costs and experimental time from 2 days to 4 h, but eradicate the FAW as soon as it enters a new area.

scholarly journals Temporal Dynamics of the Cecal Gut Microbiota of Juvenile Arctic Ground Squirrels: a Strong Litter Effect across the First Active Season

2014 ◽  
Vol 80 (14) ◽  
pp. 4260-4268 ◽  
Author(s):  
Timothy J. Stevenson ◽  
C. Loren Buck ◽  
Khrystyne N. Duddleston
Keyword(s):  
Microbial Community ◽  
Microbial Diversity ◽  
Short Chain Fatty Acid ◽  
Chain Fatty Acid ◽  
Ground Squirrels ◽  
Short Chain ◽  
Active Season ◽  
The Mean ◽  
Arctic Ground Squirrels ◽  
Gut Microbial Community

ABSTRACTArctic ground squirrels (Urocitellus parryii) are active for a scant 3 to 5 months of the year. During the active season, adult squirrels compete for mates, reproduce, and fatten in preparation for hibernation, while juvenile squirrels, weaned in early July, must grow and acquire sufficient fat to survive their first hibernation season. During hibernation, the gut microbial community is altered in diversity, abundance, and activity. To date, no studies have examined the gut microbiota of hibernators across the truncated active season. We characterized trends in diversity (454 pyrosequencing), density (flow cytometry), viability (flow cytometry), and metabolism (short-chain fatty acid analysis) of the gut microbial community of juvenile arctic ground squirrels across their first active season at weaning and at 4, 6, 8, and 10 weeks postweaning. At 8 weeks postweaning, the mean bacterial density was significantly higher than that at weaning, and the mean percentage of live bacteria was significantly higher than that at either weaning or 4 weeks postweaning. No significant differences in microbial diversity, total short-chain fatty acid concentrations, or molar proportions of individual short-chain fatty acids were observed among sample periods. The level of variability in gut microbial diversity among squirrels was high across the active season but was most similar among littermates, except at weaning, indicating strong maternal or genetic influences across development. Our results indicate that genetic or maternal influences exert profound effects on the gut microbial community of juvenile arctic ground squirrels. We did not find a correlation between host adiposity and gut microbial diversity during prehibernation fattening, likely due to a high level of variability among squirrels.

scholarly journals First Report of Dimethachlon Resistance in Field Isolates of Sclerotinia sclerotiorum on Oilseed Rape in Shaanxi Province of Northwestern China

Plant Disease ◽  
2014 ◽  
Vol 98 (4) ◽  
pp. 568-568 ◽  
Author(s):  
F. Zhou ◽  
F. X. Zhu ◽  
X. L. Zhang ◽  
A. S. Zhang
Keyword(s):  
Oilseed Rape ◽  
Sclerotinia Sclerotiorum ◽  
The United States ◽  
Northwestern China ◽  
Shaanxi Province ◽  
Resistant Isolate ◽  
Economic Losses ◽  
First Report ◽  
Field Isolates ◽  
Wide Range

Sclerotinia sclerotiorum (Lib.) de Bary is a necrotrophic fungal pathogen causing diseases in a wide range of plants, including oilseed rape (3). Substantial economic losses caused by S. sclerotiorum have been reported in the United States, Canada, Brazil, South Africa, Hungary, India, Nepal, and Japan (1). Application of fungicides is the principal tool for controlling S. sclerotiorum because of lack of high level of host resistance. Dicarboximide fungicides such as dimethachlon have been widely used to control S. sclerotiorum in recent years in China and field isolates with reduced sensitivity to dimethachlon have been reported in Jiangsu Province of eastern China (2). In order to understand the current status of dimethachlon resistance in S. sclerotiorum isolates of northwestern China, 196 and 344 isolates of S. sclerotiorum collected from oilseed rape fields in 10 counties throughout Shaanxi Province in 2011 and 2012, respectively, were assayed for sensitivity to dimethachlon using 5 μg ml–1 dimethachlon as a discriminatory dose. Mycelial plugs (6 mm in diameter) cut from the margin of a 48-h-old colony were placed in the center of petri dishes containing potato dextrose agar (PDA) amended with 5 μg ml–1 dimethachlon; PDA without fungicide served as the control. Cultures were incubated at 26°C and colony growth was measured after 72 h of incubation. Isolates that showed growth on PDA amended with fungicide were tentatively considered resistant to dimethachlon, whereas the completely inhibited isolates were considered sensitive. Results showed that 1.02% or 2 isolates of the 196 isolates collected in 2011 and 3.78% or 13 isolates of the 344 isolates collected in 2012 were resistant to dimethachlon. For all the isolates considered resistant and 42 randomly selected sensitive isolates, 50% effective concentrations (EC50) were determined on PDA amended with a series of dimethachlon concentrations. The average EC50 value of dimethachlon for sensitive isolates was 0.29 ± 0.02 μg ml–1 Resistance ratios (EC50 of resistant isolate / average EC50 of sensitive isolates) for the two resistant isolates detected in 2011 were 10.28 and 23.83, respectively, whereas resistance ratios for the 13 resistant isolates detected in 2012 ranged from 24.90 to 101.97. The average EC50 value of dimethachlon for the 13 resistant isolates detected in 2012 was 19.05 μg ml–1, and EC50 values for the two resistant isolates detected in 2011 were 2.98 and 6.91 μg ml–1, respectively. These results indicated that both resistance frequency and resistance level increased from 2011 to 2012. Bioassay results of three resistant isolates indicated that there was positive cross-resistance between dimethachlon and other dicarboximide fungicides such as iprodione and procymidone. To our knowledge, this is the first report of dimethachlon resistance in S. sclerotiorum in Shaanxi Province of northwestern China. The molecular mechanism of dimethachlon resistance in field isolates of S. sclerotiorum remains to be studied. Although resistance frequency is low at present, dimethachlon resistance should be kept in mind and fungicide resistance management tactics such as use of biological control agents, fungicide tank-mixing, or alternating dimethachlon with other fungicides having different modes of action is recommended in controlling S. sclerotiorum. References: (1) M. D. Bolton et al. Mol Plant Pathol. 7:1, 2006. (2) H. X. Ma et al. Plant Dis. 93:36, 2009. (3) L. H. Prudy. Phytopathology 69:875, 1979.

scholarly journals Development and Application of Seed Coating Agent for the Control of Major Soil-Borne Diseases Infecting Wheat

Agronomy ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 413 ◽  
Author(s):  
Ren ◽  
Chen ◽  
Ye ◽  
Su ◽  
Xiao ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
Seed Coating ◽  
Practical Application ◽  
Active Constituent ◽  
Coating Agent ◽  
Grinding Method ◽  
Fungal Inhibition ◽  
Sharp Eyespot ◽  
Coating Agents ◽  
Seed Germination Test

In order to reduce the usage amount of pesticide fertilizers and protect the natural environment, seed coating agents are receiving increased wide concern. In this study, the active constituent (pesticide) and inactive components (surfactants and film former) of the seed coating agents were screened and optimized by the wet sand processing superfine grinding method. The fungal inhibition test of pesticides showed that thifluzamide, fludioxonil, pyraclostrobin, and difenoconazole have an obvious fungal inhibitory effect on wheat sharp eyespot, take-all, and root rot. LAE-9 and polyacrylamide + carboxymethyl cellulose (CMC) is recommended for the safe surfactant and film former, respectively, based on the seed germination test. Moreover, 6% difenoconazole · fludioxonil flowable concentrate for seed coating (FSC) stimulates the seedling growth of wheat, advances the growth of root, and improves biomass in the field trial, meanwhile, the control efficiency reached above 80%. Thus, we suggested it can be used as an effective seed coating agent for the control of soil-borne diseases in wheat. The seed coating agent has the characteristics of disease prevention, increasing crop yield, and safety of environment, which is of significance in practical application.

scholarly journals Toxin Production in Soybean (Glycine max L.) Plants with Charcoal Rot Disease and by Macrophomina phaseolina, the Fungus that Causes the Disease

Toxins ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 645 ◽  
Author(s):  
Hamed K. Abbas ◽  
Nacer Bellaloui ◽  
Cesare Accinelli ◽  
James R. Smith ◽  
W. Thomas Shier
Keyword(s):  
Culture Media ◽  
Leaf Disc ◽  
Toxin Production ◽  
Macrophomina Phaseolina ◽  
Economic Losses ◽  
Limit Of Quantification ◽  
Charcoal Rot ◽  
Wide Range ◽  
Soybean Plants ◽  
Rot Disease

Charcoal rot disease, caused by the fungus Macrophomina phaseolina, results in major economic losses in soybean production in southern USA. M. phaseolina has been proposed to use the toxin (-)-botryodiplodin in its root infection mechanism to create a necrotic zone in root tissue through which fungal hyphae can readily enter the plant. The majority (51.4%) of M. phaseolina isolates from plants with charcoal rot disease produced a wide range of (-)-botryodiplodin concentrations in a culture medium (0.14–6.11 µg/mL), 37.8% produced traces below the limit of quantification (0.01 µg/mL), and 10.8% produced no detectable (-)-botryodiplodin. Some culture media with traces or no (-)-botryodiplodin were nevertheless strongly phytotoxic in soybean leaf disc cultures, consistent with the production of another unidentified toxin(s). Widely ranging (-)-botryodiplodin levels (traces to 3.14 µg/g) were also observed in the roots, but not in the aerial parts, of soybean plants naturally infected with charcoal rot disease. This is the first report of (-)-botryodiplodin in plant tissues naturally infected with charcoal rot disease. No phaseolinone was detected in M. phaseolina culture media or naturally infected soybean tissues. These results are consistent with (-)-botryodiplodin playing a role in the pathology of some, but not all, M. phaseolina isolates from soybeans with charcoal rot disease in southern USA.

scholarly journals The Coevolution of Plants and Microbes Underpins Sustainable Agriculture

2021 ◽  
Vol 9 (5) ◽  
pp. 1036
Author(s):  
Dongmei Lyu ◽  
Levini A. Msimbira ◽  
Mahtab Nazari ◽  
Mohammed Antar ◽  
Antoine Pagé ◽  
...  
Keyword(s):  
Microbial Community ◽  
Plant Growth ◽  
Stress Resistance ◽  
Mycorrhizal Fungi ◽  
Plant Tissues ◽  
Terrestrial Plants ◽  
Plant Host ◽  
Symbiotic Relationships ◽  
Wide Range ◽  
Terrestrial Environments

Terrestrial plants evolution occurred in the presence of microbes, the phytomicrobiome. The rhizosphere microbial community is the most abundant and diverse subset of the phytomicrobiome and can include both beneficial and parasitic/pathogenic microbes. Prokaryotes of the phytomicrobiome have evolved relationships with plants that range from non-dependent interactions to dependent endosymbionts. The most extreme endosymbiotic examples are the chloroplasts and mitochondria, which have become organelles and integral parts of the plant, leading to some similarity in DNA sequence between plant tissues and cyanobacteria, the prokaryotic symbiont of ancestral plants. Microbes were associated with the precursors of land plants, green algae, and helped algae transition from aquatic to terrestrial environments. In the terrestrial setting the phytomicrobiome contributes to plant growth and development by (1) establishing symbiotic relationships between plant growth-promoting microbes, including rhizobacteria and mycorrhizal fungi, (2) conferring biotic stress resistance by producing antibiotic compounds, and (3) secreting microbe-to-plant signal compounds, such as phytohormones or their analogues, that regulate aspects of plant physiology, including stress resistance. As plants have evolved, they recruited microbes to assist in the adaptation to available growing environments. Microbes serve themselves by promoting plant growth, which in turn provides microbes with nutrition (root exudates, a source of reduced carbon) and a desirable habitat (the rhizosphere or within plant tissues). The outcome of this coevolution is the diverse and metabolically rich microbial community that now exists in the rhizosphere of terrestrial plants. The holobiont, the unit made up of the phytomicrobiome and the plant host, results from this wide range of coevolved relationships. We are just beginning to appreciate the many ways in which this complex and subtle coevolution acts in agricultural systems.

scholarly journals Integration of early disease-resistance phenotyping, histological characterization, and transcriptome sequencing reveals insights into downy mildew resistance in impatiens

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Ze Peng ◽  
Yanhong He ◽  
Saroj Parajuli ◽  
Qian You ◽  
Weining Wang ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Downy Mildew ◽  
Transcriptome Sequencing ◽  
Early Stage ◽  
Economic Losses ◽  
Potential Candidate ◽  
Early Disease ◽  
True Leaf ◽  
Cotyledon Stage ◽  
Wide Range

AbstractDowny mildew (DM), caused by obligate parasitic oomycetes, is a destructive disease for a wide range of crops worldwide. Recent outbreaks of impatiens downy mildew (IDM) in many countries have caused huge economic losses. A system to reveal plant–pathogen interactions in the early stage of infection and quickly assess resistance/susceptibility of plants to DM is desired. In this study, we established an early and rapid system to achieve these goals using impatiens as a model. Thirty-two cultivars of Impatiens walleriana and I. hawkeri were evaluated for their responses to IDM at cotyledon, first/second pair of true leaf, and mature plant stages. All I. walleriana cultivars were highly susceptible to IDM. While all I. hawkeri cultivars were resistant to IDM starting at the first true leaf stage, many (14/16) were susceptible to IDM at the cotyledon stage. Two cultivars showed resistance even at the cotyledon stage. Histological characterization showed that the resistance mechanism of the I. hawkeri cultivars resembles that in grapevine and type II resistance in sunflower. By integrating full-length transcriptome sequencing (Iso-Seq) and RNA-Seq, we constructed the first reference transcriptome for Impatiens comprised of 48,758 sequences with an N50 length of 2060 bp. Comparative transcriptome and qRT-PCR analyses revealed strong candidate genes for IDM resistance, including three resistance genes orthologous to the sunflower gene RGC203, a potential candidate associated with DM resistance. Our approach of integrating early disease-resistance phenotyping, histological characterization, and transcriptome analysis lay a solid foundation to improve DM resistance in impatiens and may provide a model for other crops.

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