Selectivity of Entomopathogenic Fungi to Chrysoperla externa (Neuroptera: Chrysopidae)

We aimed to evaluate the selectivity of entomopathogenic fungi to larvae of Chrysoperla externa (Neuroptera: Chrysopidae). For this purpose, Beauveria bassiana (strain ESALQ PL63), Metarhizium anisopliae (strain ESALQ E9) and Metarhizium rileyi (strain UFMS 03) were assessed at different concentrations (1 × 107, 1 × 108 and 1 × 109 conidia mL−1). The control treatment consisted of distilled water and Tween80 0.01. The treatments were applied with a Potter spray tower using two different methodologies: direct application (DA) and dry film (DF). Up to 96 h after application, no treatment provided a larval mortality above 3%. After 120 h, only B. bassiana induced significant mortality in all instars, with rates of 26%, 17% and 10% for first, second and third instar larval periods, respectively. There was no difference regarding to the application method or concentration of conidia. The percentage of individuals that revealed changes in the length of the larval and pupal periods varied among different treatments with entomopathogenic fungi and control treatments, application methodologies and concentrations. Despite B. bassiana revealing a higher mortality than M. anisopliae and M. rileyi on larvae of C. externa, these three entomopathogenic fungi may be used in association with C. externa for sustainable pest management.

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Effect of biofertilizers and diatomaceous earth on life and movement of subterranean termites under laboratory conditions

International Journal of Tropical Insect Science ◽

10.1017/s1742758418000103 ◽

2018 ◽

Vol 38 (04) ◽

pp. 348-352

Author(s):

Sohail Ahmed ◽

Babar Hassan ◽

Muhammad Umer Farooq

Keyword(s):

Toxic Elements ◽

Diatomaceous Earth ◽

The Other ◽

Control Treatment ◽

Subterranean Termites ◽

Soil Substrate ◽

Laboratory Conditions ◽

And Control ◽

Significant Mortality

AbstractThe presence of biofertilizers can affect the life and movement of subterranean organisms in the soil. In the current study, the effect of three biofertilizers (Unigrow, Rhizogold and Rhizogold Plus) alone and in combination with diatomaceous earth (DE) and insecticides on mortality and movement of subterranean termites, Coptotermes heimi (Wasmann), was investigated under laboratory conditions. The mortality of termites in soil mixed with biofertilizers was low as compared to the control treatment, which led to significant mortality. Length of galleries formed by termites with the addition of Unigrow in the soil was significantly higher as compared to the other two biofertilizers and control treatments. Mortality increased in the presence of insecticides and DE in the soil substrate. Unigrow mixed soil having chlorfenapyr had maximum mortality and minimum gallery length followed by Rhizogold and Rhizogold Plus. The addition of DE and insecticide further enhances mortality, and we recommend that some toxic elements should be incorporated in biofertilizers to control subterranean termites.

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Effect of Iron Fortified Wheat Flour on the Biology and Physiology of Red Flour Beetle, Tribolium castaneum (Herbst)

International Journal of Insect Science ◽

10.4137/ijis.s5200 ◽

2010 ◽

Vol 2 ◽

pp. IJIS.S5200 ◽

Cited By ~ 1

Author(s):

Sohail Ahmed ◽

Waqas Wakil ◽

H.M. Salman Saleem ◽

Mohammad Shahid ◽

M. Usman Ghazanfar

Keyword(s):

Tribolium Castaneum ◽

First Generation ◽

Larval Mortality ◽

Developmental Time ◽

Red Flour Beetle ◽

Control Treatment ◽

Larval Weight ◽

Flour Beetle ◽

Fortified Flour ◽

And Control

Iron overload in the fortified flour can influence the life stages and physiology of the insects. The present study was carried out to evaluate the effect of commercially available premix iron fortified flour as well as effect of different concentrations of post-mix iron fortified flour (30–5 ppm) on biology of red flour beetle, Tribolium castaneum (Hebrst.). Larval and pupal duration, total developmental time, fecundity and larval weights in two consecutive generations of beetle were compared with control treatment. Amylase and protease activities of gut of the beetle were also measured in premix and postmix flours. Results showed that larval mortality increased in two sources of premix iron flour when compared with control. Larval weight was reduced in first generation only. The larval mortality was significantly higher in 30 ppm postmix iron fortified flour than in other postmix concentrations and control treatment. The larvae of T. castaneum fed on two sources of premix and in various concentrations of postmix iron fortified flour revealed an increase in amylases and decrease in protease activities.

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Pathogenicity of Metarhizium rileyi (Farlow) Kepler, S.A. Rehner and Humber isolates against Spodoptera litura (Fabricius) and their extracellular enzymatic activities

Egyptian Journal of Biological Pest Control ◽

10.1186/s41938-021-00407-4 ◽

2021 ◽

Vol 31 (1) ◽

Author(s):

Gurmehar Kaur Grewal ◽

Neelam Joshi ◽

Yadhu Suneja

Keyword(s):

Spodoptera Litura ◽

Larval Mortality ◽

Screening Methods ◽

Metarhizium Rileyi ◽

Percent Mortality ◽

Commercially Important ◽

Extracellular Enzymatic Activities ◽

Chemical Pesticides ◽

Lepidoptera Noctuidae

Abstract Background Spodoptera litura (Fab.) (Lepidoptera: Noctuidae) is a serious agricultural pest that infests many commercially important crops of Southeast Asian countries. Indiscriminate use of chemical pesticides has led to various health hazards as well as insecticide resistance. Entomopathogenic fungi (EPF) provide an important alternative as biological control agents. Metarhizium rileyi is an EPF with a specific host range for lepidopteran pests. The present study aimed to identify virulent M. rileyi isolate against S. litura larvae and analyse their extracellular cuticle-degrading enzyme activities. Results Three M. rileyi isolates viz M. rileyi NIPHM, M. rileyi MTCC 4254 and M. rileyi MTCC 10395 formulations were evaluated at different concentrations against 2nd instar larvae of S. litura. A maximum percent mortality of 63.33% was recorded in M. rileyi NIPHM (12 g/l), followed by M. rileyi MTCC 4254 (58.33%) at the same concentration, 10 days post-treatment. Maximum means of chitinase, protease and lipase activities (0.44, 1.58 and 2.95 U/ml, respectively) were recorded in the case of M. rileyi NIPHM. Correlation analysis was positive between enzyme activity and larval mortality. Conclusions Metarhizium rileyi NIPHM recorded the highest enzymatic activity and exhibited the maximum mortality rate against 2nd instar larvae of S. litura, suggesting the possible role of these enzymes in the pathogenicity of the fungus. Further knowledge in this regard may help in the development of enzyme-based screening methods for selecting virulent fungal isolates for the eco-friendly management of crop pests.

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The effect of mineral fertilisers and farmyard manure on grain and straw yield, quality and economical parameters of winter wheat

Plant Soil and Environment ◽

10.17221/60/2020-pse ◽

2020 ◽

Vol 66 (No. 6) ◽

pp. 249-256

Author(s):

Lukáš Hlisnikovský ◽

Milan Vach ◽

Zdeněk Abrhám ◽

Ladislav Mensik ◽

Eva Kunzová

Keyword(s):

Winter Wheat ◽

Farmyard Manure ◽

Market Price ◽

Economic Benefits ◽

Quality Parameters ◽

Control Treatment ◽

Yield Quality ◽

Technology And Economy ◽

And Control ◽

Technological Methods

In the years 2011–2014, winter wheat grain yield, qualitative and economic parameters were evaluated according to different fertiliser treatments: (1) control: unfertilised treatment; (2) farmyard manure (FYM) and (3) FYM + NPK (farmyard manure applied together with mineral NPK). The highest yields (8.10 t/ha) were recorded in the FYM + NPK treatment, while significantly lower yields (6.20 t/ha and 5.73 t/ha) were recorded in FYM and control treatments, respectively. Similarly, statistically significantly higher values of the quality parameters were found in the FYM + NPK treatment (13.55% of crude protein content and 43.56 mL of Zeleny’s sedimentation test), compared to control (10% and 22.44 mL, respectively). The modelling expert system (AGROTEKIS-Crop Technology and Economy) was used for the evaluation of economy. This software is based on technological methods of cultivation and norms of material input costs and costs of individual mechanised works. The economic benefits and profitability were evaluated for three different levels of grain market price. The highest gross profit per ha was recorded in the FYM + NPK treatment. According to the gross profit, the control treatment provided better results than the FYM treatment.

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Effect of Ethephon and Packaging Practices On Ripening of Mango cv. Maldah

SAARC Journal of Agriculture ◽

10.3329/sja.v19i1.54786 ◽

2021 ◽

Vol 19 (1) ◽

pp. 155-163

Author(s):

S Sapkota ◽

D Kc ◽

H Giri ◽

M Saud ◽

M Basnet ◽

...

Keyword(s):

Chemical Properties ◽

Control Treatment ◽

Quality Performance ◽

Plastic Packaging ◽

Randomized Design ◽

Acid Ratio ◽

Ethephon Treatment ◽

Completely Randomized Design ◽

And Control ◽

Juice Content

The present research was conducted on two factor Completely Randomized Design (CRD) with eight treatments and three replications. A set of experiments were carried out to evaluate the effect of postharvest ethephon treatment and packaging on ripening of mango cv. Maldah. The treatments consisted of ripening agent i.e., ethephon and control treatment under different packaging condition i.e., fiber with hole, fiber without hole, plastic with hole and plastic without hole. The result revealed that different packaging condition and ripening agents influenced the ripening behavior of mango. The highest TSS (15.26), sugar-acid ratio (23.66) and juice content (126.05) were recorded with fiber (without hole) and the lowest TSS (12.60), sugar-acid ratio (9.01) and juice content (116.05) with plastic (without hole). The highest TA (1.44) was recorded with plastic (without hole) and the lowest (0.66) with fiber (without hole). Similarly, the highest BT (2.83) was recorded with fiber (with hole) and the lowest (1.66) with plastic (without hole). Firmness, sweetness, TSS and juice content were the highest with the interaction effect of fiber bag (without hole) and ethephon treatment. In conclusion, mango fruits with ethephon treatment packed in fiber bag (without hole) enhances quality and ripening of mango whereas under controlled condition and without hole plastic packaging mangoes had low quality performance in terms of physio-chemical properties. SAARC J. Agric., 19(1): 155-163 (2021)

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Evaluating the virulence of Metarhizium anisopliae (Deuteromycotina: Hyphomycetes) and Beauveria bassiana (Ascomycota: Hypocreales) isolates to Arabian rhinoceros beetle, Oryctes agamemnon arabicus

Journal of Entomological and Acarological Research ◽

10.4081/jear.2015.5180 ◽

2015 ◽

Vol 47 (3) ◽

pp. 117 ◽

Cited By ~ 1

Author(s):

M.W. Khudhair ◽

M.Z. Khalaf ◽

H.F. Alrubeai ◽

A.K. Shbar ◽

B.S. Hamad ◽

...

Keyword(s):

Mortality Rate ◽

Beauveria Bassiana ◽

Entomopathogenic Fungi ◽

Metarhizium Anisopliae ◽

Pupal Stage ◽

Control Treatment ◽

Rhinoceros Beetle ◽

Oryctes Agamemnon ◽

Lethal Time ◽

Short Time

Virulence of entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana were tested against Arabian Rhinoceros Beetle, Oryctes agamemnon arabicus larvae. Four concentrations (1×105, 1×107, 1×109 and 1×1011 conidia/mL–1) of two locally isolated entomopathogenic fungi spore suspensions were used in this study via larval direct spraying. Results revealed that both isolates can cause high mortality rate reaching 100% after 29 days. However, Beauveria bassiana scored higher mortality rate in short time especially at the concentration of 1×1011 conidia/ mL–1 with lethal time (LT)50 12.75 and LT90 20.00; while, Metarhizium anisopliae caused the higher percentage of malformed adults. Moreover, both isolates affected insect’s life cycle particularly in the pupal stage which was reduced remarkably by almost 50% in comparison with the control treatment.

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Frequency in the supply of Lactobacillus influence its probiotic effect for yellow tail lambari

Ciência Rural ◽

10.1590/0103-8478cr20180042 ◽

2018 ◽

Vol 48 (10) ◽

Cited By ~ 4

Author(s):

Adolfo Jatobá ◽

Klayton Natan Moraes ◽

Emanuel Fernandes Rodrigues ◽

Leandro Marcolino Vieira ◽

Marina Oliveira Pereira

Keyword(s):

Intestinal Tract ◽

Leukocyte Count ◽

Hematological Parameters ◽

Control Treatment ◽

Productive Performance ◽

Probiotic Lactobacillus ◽

Final Weight ◽

And Control ◽

Lactobacillus Spp ◽

Probiotic Effect

ABSTRACT: The aim of this research was to evaluate the changes in intestinal tract microbiota, hematological parameters and zootechnical indexes of yellowtail lambaris (Astyanax bimaculatus) fed with different probiotic supply frequencies (Lactobacillus spp.). Lambaris (1,200) were distributed in twelve boxes, divided in three treatments, 100% supply; 25% and 0% (control) of feeds supplemented with probiotic. After 87 days, fish fed with supplemented diet, regardless of frequency (100% and 25%), showed an increase in the total lactic acid bacteria count of 7.99±0.15 log CFU mL-1 and 7.04±1, 29 log UFC mL-¹ respectively, in the intestinal tract and decrease of Pseudomonas spp. in both 100% and 25% (4.09±0.84log UFC mL-1 and 4.00±1.12log UFC mL-1, respectively) in relation to the control treatment (6.74±1.04log UFC mL-1). Fish fed with 100% of diets supplemented with probiotics had a higher leukocyte count (47.7±6.7x103μL-1), lymphocytes (36.0±2.6x103μL1), monocytes (12.0±4.2x103μl-1), as well as higher mean final weight (10.60±0.89g) and weekly growth rate (0.80±0.08g.week-1) than the other treatments (25% of probiotic offered and control). Probiotic (Lactobacillus spp.) offered in 25% of feeds (or more) alters the yellowtail lambari microbiota (A. bimaculatus); however, must be offer in 100% of feeds to improve the hematology profile and productive performance.

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Effectiveness of Selected Entomopathogenic Fungi in Packed Rice Grain at Room Temperature Against Corcyra Cephalonica Stainton

ASEAN Journal on Science and Technology for Development ◽

10.29037/ajstd.103 ◽

2017 ◽

Vol 23 (3) ◽

pp. 183 ◽

Cited By ~ 6

Author(s):

Hendrawan Samodra ◽

Yusof Bin Ibrahim

Keyword(s):

Beauveria Bassiana ◽

High Mortality ◽

Entomopathogenic Fungi ◽

Metarhizium Anisopliae ◽

Room Temperature ◽

Larval Mortality ◽

Corcyra Cephalonica ◽

Rice Grain ◽

Rice Moth ◽

Tapioca Flour

Eight isolates of entomopathogenic fungi were evaluated as dried conidia against the rice moth, Corcyra cephalonica. In bioassays two isolates of Beauveria bassiana (BbGc and BbPs) and one isolate of Metarhizium anisopliae (MaPs) consistently gave high mortality to C. cephalonica larvae. Formulations in either kaolin, talc or tapioca flour (20 % w/w a.i.) thoroughly mixed with long grain rice in plastic cups (8 cm diameter by 5 cm) gave complete larval mortality by the 12th day of treatment. However, in general those formulated in kaolin and talc were more efficacious and faster to kill compared to those formulated in tapioca flour or the unformulated control. Even at the lowest rate of 0.05 g BbGc in kaolin provided 100% mortality 7 days after introduction compared with other dust formulations. Isolate BbGc in kaolin and talc administered at 0.4 g a.i. in 200 g rice packed in plastic kept at room temperature provided protection against the rice moth up to 4 months of storage. Larval mortality in excess of 90% was obtained 15 days after introduction. Formulations of MaPs was effective only within the first month of storage beyond which infectivity rapidly declined.

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Nondetrimental impact of two concomitant entomopathogenic fungi on life history parameters of a generalist predator, Coccinella septempunctata (Coleoptera: Coccinellidae)

Scientific Reports ◽

10.1038/s41598-021-00037-8 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Muhammad Rizwan ◽

Bilal Atta ◽

Muhammad Arshad ◽

Rashad Rasool Khan ◽

Asli Dageri ◽

...

Keyword(s):

Side Effects ◽

Life Table ◽

Entomopathogenic Fungi ◽

Coccinella Septempunctata ◽

Reproductive Rate ◽

Control Treatment ◽

Generalist Predator ◽

Life Table Parameters ◽

Net Reproductive Rate ◽

Fecundity Rate

AbstractThe non-persistent impact of biocontrol agents can be revealed for pest control when associated entomopathogenic fungi (EPFs) negatively affect the natural enemies. In this assay, impacts of Beauvaria bassiana (Balsamo) Vuillemin, and Metarhizium anisopliae (Metschnikoff) Sorokin were studied for their compatibility or side effects on life table parameters of an important generalist predator, Coccinella septempunctata L. The results indicated non-significant impacts of both EPFs on life table parameters of C. septempunctata. The development time (egg-adult) was not significantly different in control (69.79 days) and EPFs treated C. septempunctata (69.35–80.07 days). Both fungi did not induce any significant changes in the fecundity, adult pre-oviposition period (APOP), total preoviposition period (TPOP), and mean generation time (T) as compared to control treatment. Similarly, no difference in fecundity rate of C. septempunctata was observed after EPFs treatment (287.7–288.5) compared to control (290.0). The highest net reproductive rate (R0) occurred in control (87.05 offspring individual−1) and M. anisopliae (86.31 offspring individual−1) as compared to B. bassiana treated beetles (76.97 offspring individual−1). The age-specific fecundity curves indicated that the C. septempunctata had a similar fecundity rate in both EPFs treatments and control. This study demonstrates no significant side effects of B. bassiana and M. anispoliae on the performance and biology of C. septempunctata. Considering the compatibility of both EPFs with C. septempunctata, their combinations can be recommended in various integrated pest management programs.

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Foliar silicate application improves the tolerance of celery grown under heat stress conditions

Acta agriculturae Slovenica ◽

10.14720/aas.2021.117.3.1865 ◽

2021 ◽

Vol 117 (3) ◽

pp. 1

Author(s):

Fadl Abdelhamid HASHEM ◽

Rasha M. EL-MORSHEDY ◽

Tarek M. YOUNIS ◽

Mohamed A. A. ABDRABBO

Keyword(s):

Heat Stress ◽

Vegetative Growth ◽

Foliar Application ◽

Climate Change Impacts ◽

Potassium Silicate ◽

Growth And Yield ◽

Apium Graveolens ◽

Control Treatment ◽

F1 Hybrid ◽

And Control

Temperature rise is one of the most challenging climate change impacts that increase the intensity of heat stress. In this investigated the production of celery (Apium graveolens var. rapaceum F1 hybrid)) was tested during the late season. The experiment was carried out during the two successive summer seasons of 2019 and 2020 in Giza Governorate, Egypt. The experimental design is a split-plot, the main plots consist of three low tunnel cover treatments, and three spray treatments with three replicates in sub-main plots. Results showed that the use of white net cover gave the highest vegetative growth and yield followed by the black net. Values of plant yield were 951, 765, and 660 g/plant for white, black and without cover, respectively, in the first season. The foliar application of 3 mM of potassium silicate produced the highest vegetative growth and yield compared to the control treatment. Referring to the effect of spray foliar application of potassium silicate on yield 1.5 mM (S1), 3 mM (S2), and control were 892, 795, and 689 g/plant in the first season, respectively. The best combination that delivered the highest vegetative growth and yield was a cover low tunnel with a white net combined with S2 foliar application.

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