scholarly journals Frequency in the supply of Lactobacillus influence its probiotic effect for yellow tail lambari

2018 ◽  
Vol 48 (10) ◽  
Author(s):  
Adolfo Jatobá ◽  
Klayton Natan Moraes ◽  
Emanuel Fernandes Rodrigues ◽  
Leandro Marcolino Vieira ◽  
Marina Oliveira Pereira

ABSTRACT: The aim of this research was to evaluate the changes in intestinal tract microbiota, hematological parameters and zootechnical indexes of yellowtail lambaris (Astyanax bimaculatus) fed with different probiotic supply frequencies (Lactobacillus spp.). Lambaris (1,200) were distributed in twelve boxes, divided in three treatments, 100% supply; 25% and 0% (control) of feeds supplemented with probiotic. After 87 days, fish fed with supplemented diet, regardless of frequency (100% and 25%), showed an increase in the total lactic acid bacteria count of 7.99±0.15 log CFU mL-1 and 7.04±1, 29 log UFC mL-¹ respectively, in the intestinal tract and decrease of Pseudomonas spp. in both 100% and 25% (4.09±0.84log UFC mL-1 and 4.00±1.12log UFC mL-1, respectively) in relation to the control treatment (6.74±1.04log UFC mL-1). Fish fed with 100% of diets supplemented with probiotics had a higher leukocyte count (47.7±6.7x103μL-1), lymphocytes (36.0±2.6x103μL1), monocytes (12.0±4.2x103μl-1), as well as higher mean final weight (10.60±0.89g) and weekly growth rate (0.80±0.08g.week-1) than the other treatments (25% of probiotic offered and control). Probiotic (Lactobacillus spp.) offered in 25% of feeds (or more) alters the yellowtail lambari microbiota (A. bimaculatus); however, must be offer in 100% of feeds to improve the hematology profile and productive performance.

2020 ◽  
Vol 6 (2) ◽  
pp. 311-315
Author(s):  
Manash Kabiraj ◽  
Prosenzit Kumar Das ◽  
Shamima Sultana ◽  
Ghausiatur Reza Banu

The research was aimed to determine the present status of probiotics (Lactobacillus spp.) and pathogenic bacteria (Vibrio spp.) of culture pond as well as to determine whether the isolated probiotic Lactobacillus spp. can act as a controlling agent on experimentally pathogenic Vibrio spp. infected Penaeus monodon. In In-vitro test of gills and intestinal tracts, the potential antagonistic activity of Lactobacillus spp. toward Vibrio spp. was gradually observed at 4th hour of probiotic treatment. At 12th hour the viable count of Vibrio spp. was drastically reduced in gill from 2.33×105 to 1.03×103 (CFUg-1) and in intestinal tract 2.35×105 to 6.43×102 (CFUg-1). While, in In-vivo test, in case of muscle, gills and intestinal tract antagonistic activity of Lactobacillus spp. toward Vibrio spp. was noticed after 9 hours, 21 hours and 27 hours respectably of probiotic injected shrimps. However, only the result of in-vitro challenge test revealed that, Lactobacillus spp. significantly reduced the Vibrio spp. viable count of the gills of the selected samples (P=0.037). The investigation showed antagonistic effect of probiotic (Lactobacillus spp.) on experimentally Vibrio spp. infected shrimp. Asian J. Med. Biol. Res. June 2020, 6(2): 311-315


2019 ◽  
Vol 8 (1) ◽  
pp. 951
Author(s):  
Achmad Noerkhaerin Putra ◽  
Anngy Chahya Pradana ◽  
Deny Novriansyah ◽  
Mustahal Mustahal

Leucaena leaves is a potential ingredient for raw material feed of catfish. The aim of this study is to evaluate the effects of dietary fermented leucaena leaf on digestibility and hematological parameters of catfish. Four treatments and 3 replicates, namely: A (reference feed 70% + leucaena leaves meal 30%), B (reference feed 70%+fermented leucaena leaves meal with A. niger 30%), C (reference feed 70%+fermented leucaena leaves meal with R. oligosporus 30%), and feed D (reference feed 70%+fermented leucaena leaves meal with S. cerevisiae 30%) were used in this study. The juvenile catfish (initial weight was 5,45±0,16 g) are randomly distributed into eighteen tanks with 40 fish per aquarium. Each diet is randomly assigned to the triplicate aquarium and fed to fish three times a day up to satiation for 4 weeks. The results showed that fermented leucaena leaves treatments were significantly higher (P<0.05) than that control in final weight and feed conversion rasio. Nutrients digestibility and final weight were significantly highest (P<0.05) in fermented leucaena leaves with A. niger compared to the other treatment. The value of protein digestibility was significantly highest (P<0.05) in fermented leucaena leaves with A. niger (76,04%), followed by fermented leucaena leaves S. cerevisiae with (69,71%), fermented leucaena leaves meal with R. oligosporus (68,24%), and control (65,18). Leucaena leaves had no effect on physiological processes in catfish, as shown by hematological parameter values that were within the normal range.


2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Natalino Da Costa Sousa ◽  
Márcia Valéria Silva do Couto ◽  
Peterson Emmanuel Guimarães Paixão ◽  
Estela dos Santos Medeiros ◽  
João Carlos Nunes de Souza ◽  
...  

This study evaluated the effect of enriched artemia nauplii with commercial probiotic for angelfish larvae determining productive performance, intestinal modulation and survival. Therefore, it experiment occurred in completely randomized design with five treatments (T1- 0.0, T2- 1.5, T3- 3.0, T4- 4.5 and T5- 6.0g of commercial probiotic) and four replaces during 20 days. After larvaculture, post larvae passed by biometric procedures to determine productive performance and then microbiological analysis. Occurred reduction of total heterotrophic bacteria while increased lactic acid bacteria in the intestinal tract from the post larvae for treatments T3, T4 and T5. The commercial probiotic also increased the survival and performance as final weight, weight gain and specific growth rate. For these reasons, the use of 3g of commercial probiotic promotes greater performance and intestinal modulation for angelfish larvae.


2020 ◽  
Vol 66 (No. 6) ◽  
pp. 249-256
Author(s):  
Lukáš Hlisnikovský ◽  
Milan Vach ◽  
Zdeněk Abrhám ◽  
Ladislav Mensik ◽  
Eva Kunzová

In the years 2011–2014, winter wheat grain yield, qualitative and economic parameters were evaluated according to different fertiliser treatments: (1) control: unfertilised treatment; (2) farmyard manure (FYM) and (3) FYM + NPK (farmyard manure applied together with mineral NPK). The highest yields (8.10 t/ha) were recorded in the FYM + NPK treatment, while significantly lower yields (6.20 t/ha and 5.73 t/ha) were recorded in FYM and control treatments, respectively. Similarly, statistically significantly higher values of the quality parameters were found in the FYM + NPK treatment (13.55% of crude protein content and 43.56 mL of Zeleny’s sedimentation test), compared to control (10% and 22.44 mL, respectively). The modelling expert system (AGROTEKIS-Crop Technology and Economy) was used for the evaluation of economy. This software is based on technological methods of cultivation and norms of material input costs and costs of individual mechanised works. The economic benefits and profitability were evaluated for three different levels of grain market price. The highest gross profit per ha was recorded in the FYM + NPK treatment. According to the gross profit, the control treatment provided better results than the FYM treatment.


2021 ◽  
Vol 19 (1) ◽  
pp. 155-163
Author(s):  
S Sapkota ◽  
D Kc ◽  
H Giri ◽  
M Saud ◽  
M Basnet ◽  
...  

The present research was conducted on two factor Completely Randomized Design (CRD) with eight treatments and three replications. A set of experiments were carried out to evaluate the effect of postharvest ethephon treatment and packaging on ripening of mango cv. Maldah. The treatments consisted of ripening agent i.e., ethephon and control treatment under different packaging condition i.e., fiber with hole, fiber without hole, plastic with hole and plastic without hole. The result revealed that different packaging condition and ripening agents influenced the ripening behavior of mango. The highest TSS (15.26), sugar-acid ratio (23.66) and juice content (126.05) were recorded with fiber (without hole) and the lowest TSS (12.60), sugar-acid ratio (9.01) and juice content (116.05) with plastic (without hole). The highest TA (1.44) was recorded with plastic (without hole) and the lowest (0.66) with fiber (without hole). Similarly, the highest BT (2.83) was recorded with fiber (with hole) and the lowest (1.66) with plastic (without hole). Firmness, sweetness, TSS and juice content were the highest with the interaction effect of fiber bag (without hole) and ethephon treatment. In conclusion, mango fruits with ethephon treatment packed in fiber bag (without hole) enhances quality and ripening of mango whereas under controlled condition and without hole plastic packaging mangoes had low quality performance in terms of physio-chemical properties. SAARC J. Agric., 19(1): 155-163 (2021)


2012 ◽  
Vol 64 (1) ◽  
pp. 192-197 ◽  
Author(s):  
R.M.R. Sousa ◽  
C.A. Agostinho ◽  
F.A. Oliveira ◽  
D. Argentim ◽  
P.K. Novelli ◽  
...  

The performance of Nile tilapia (Oreochromis niloticus) raised in cages furnished with an automatic dispenser, supplied at different frequencies (once per hour and once every two hours) and periods (daytime, nighttime and both) was evaluated. Eighteen 1.0m³ cages were placed into a 2000m² pond, two meters deep with a 5% water exchange. One hundred and seventy tilapias, with initial weight of 16.0±4.9g, were dispersed into each 1m³ cage and the feed ration was adjusted every 21 days with biometry. Data was collected from March to July (autumn and winter). Significant difference to final weight (P<0.05) among treatments was observed. The increase in feeding frequency improves the productive performance of Nile tilapias in cages and permitted better management of the food. The better feed conversion rate for high feeding frequency (24 times day-1) can result in saving up to 360kg of food for each ton of fish produced, increasing the economic sustenance for tilapia culture and suggesting less environmental pollution.


2021 ◽  
Vol 117 (3) ◽  
pp. 1
Author(s):  
Fadl Abdelhamid HASHEM ◽  
Rasha M. EL-MORSHEDY ◽  
Tarek M. YOUNIS ◽  
Mohamed A. A. ABDRABBO

<p>Temperature rise is one of the most challenging climate change impacts that increase the intensity of heat stress. In this investigated the production of celery (<em>Apium graveolens</em> var. <em>rapaceum </em>F1 hybrid)) was tested during the late season. The experiment was carried out during the two successive summer seasons of 2019 and 2020 in Giza Governorate, Egypt. The experimental design is a split-plot, the main plots consist of three low tunnel cover treatments, and three spray treatments with three replicates in sub-main plots. Results showed that the use of white net cover gave the highest vegetative growth and yield followed by the black net. Values of plant yield were 951, 765, and 660 g/plant for white, black and without cover, respectively, in the first season. The foliar application of 3 mM of potassium silicate produced the highest vegetative growth and yield compared to the control treatment. Referring to the effect of spray foliar application of potassium silicate on yield 1.5 mM (S1), 3 mM (S2), and control were 892, 795, and 689 g/plant in the first season, respectively. The best combination that delivered the highest vegetative growth and yield was a cover low tunnel with a white net combined with S2 foliar application.</p>


2014 ◽  
Vol 30 (4) ◽  
pp. 669-676 ◽  
Author(s):  
N. Puvaca ◽  
Lj. Kostadinovic ◽  
D. Ljubojevic ◽  
D. Lukac ◽  
S. Popovic ◽  
...  

The aim of this study was to investigate the effect of dietary garlic powder addition on productive performance and blood lipid status of broiler chicken. At the beginning of experiment, three treatments of 150 one day old broiler chickens of hybrid line Hubbard per treatment, on a total of 450 chickens were formed. Every treatment was divided in four groups which represents four replicates of the experiment. Control treatment (T1) was fed with mixtures without addition of garlic powder, while experimental treatments were fed with addition of 0.5% (T2) and 1.0% (T3) of dietary garlic powder, respectively. Experiment lasted 42 days. After the completion of experimental period the highest achieved body weight of chicken was at treatment T2 (2371.1g) which was followed by treatment T3 (2336.1 g) with statistically significant differences (p<0.05) compared to control treatment. For the entire experimental period, feed conversion ratio was lowest in treatment T2 (1.8 kg/kg) and the highest in control treatment T1 (2.1 kg/kg), without statistically significant (p>0.05) differences. Addition of garlic powder led to a statistically significant (p<0.05) increase in values of EBI in compare to a control treatment T1. The highest mortality rate (5.1 %) and the lowest EBI (220.4 %) were recorded in control treatment. Addition of garlic powder in the amount of 1.0% (T3) significantly (p<0.05) decreased LDL concentrations in blood serum. The lowest concentration of total cholesterol was recorded at treatment T2 (p<0.05). The highest concentration of HDL (44.8 and 39.6 mg/dl) was recorded in treatments T3 and T2. It could be concluded that the addition of garlic has positive influence on chicken production and blood lipid status, but the further investigation of their mode of action is still necessary.


Author(s):  
Tran Thanh Thai ◽  
Pham Thanh Luu ◽  
Ngo Xuan Quang ◽  
Dao Thanh Son

This study aimed to enhance our insight on the potential toxicological effects of silver nanoparticles (AgNPs)  into the aquatic environment. To investigate the chronic toxicity of nanoparticles, freshwater micro-crustacean Daphnia lumholtzi was exposed to different concentrations of 0.2, 0.5 µg/l AgNPs, and control, for 21 days. Toxicological endpoints at different growing stages such as the maturation and reproduction were recorded. The reproduction rate of D. lumholtzi exposed to both AgNPs concentrations (0.2 and 0.5 µg/l ) was significantly lower than that of control. In turn, the maturation exposed to both AgNPs concentrations was not significantly different from the control treatment. This result indicates that AgNPs (with a concentration lower than 0.5 µg/l) did not have an adverse effect on the maturation of D. lumholtzi, but AgNPs with a concentration higher than 0.2 caused a toxic effect on the reproduction rate of D. lumholtzi during 21 days of the exposure period. In conclusion, the present results showed that AgNPs have toxic effects on D. lumholtzi and it has the potential to use as good freshwater aquatic zooplankton for assessment on the toxicity of nanomaterials in tropics. The future study should pay more attention to the effect of AgNPs on survival, growth rate, and multiple generations of daphnids to better understand the effects of nanoparticles in general and AgNPs in particular.


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