scholarly journals The Imbalance among Oxidative Biomarkers and Antioxidant Defense Systems in Thromboangiitis Obliterans (Winiwarter-Buerger Disease)

2020 ◽  
Vol 9 (4) ◽  
pp. 1036
Author(s):  
Hiva Sharebiani ◽  
Bahare Fazeli ◽  
Rosanna Maniscalco ◽  
Daniela Ligi ◽  
Ferdinando Mannello
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Serum Level ◽  
Antioxidant Capacity ◽  
Smoking Habit ◽  
Thromboangiitis Obliterans ◽  
Control Group ◽  
Healthy Smoker ◽  
Significant Difference ◽  
Antioxidant Defense Systems

(1) Background: Thromboangiitis obliterans or Winiwarter-Buerger disease (WBD), is an inflammatory, thrombotic occlusive, peripheral vascular disease, usually occurring in young smokers. The pathophysiological mechanisms underlying the disease are not clearly understood. The aim of this study is to investigate the imbalance between oxidants and antioxidants occurring in these patients. (2) Patients and Methods: In this cross-sectional study, 22 male patients with WBD and 20 healthy male smoking habit matched control group were included. To evaluate the possible sources of oxidative stress, the antioxidant biomarkers, and the markers of lipid peroxidation and protein oxidation, serum samples were analyzed for total oxidative status (TOS), total antioxidant capacity (TAC), myeloperoxidase (MPO), coenzyme Q10 (CoQ10), superoxide dismutase (SOD), glutathione reductase (GR), malondialdehyde (MDA), and protein carbonyl (PC) activity and/or content. (3) Results: The circulating levels of TOS, TAC, and CoQ10 were significantly higher in WBD patients, with respect to healthy smokers as controls. No significant difference was found among the serum level of PC, total cholesterol, MPO, and GR activity in WBD patients and healthy smoker controls. The activity of SOD and the mean serum level of MDA were significantly lower in WBD patients, with respect to healthy smoker controls. (4) Conclusion: Considerably high levels of oxidative stress were detected in WBD patients, which were greater than the antioxidant capacity. The low level of MDA may be associated with the enzymatic degradation of lipid peroxidation products. High levels of CoQ10 and low levels of SOD may be related to a harmful oxidative cooperation, leading to the vasoconstriction of WBD, representing a promising tool to discern possible different clinical risks of this poorly understood peripheral occlusive disease.

scholarly journals Evaluation of Salivary and Serum Total Antioxidant Capacity and Lipid Peroxidation in Postmenopausal Women

2020 ◽  
Vol 2020 ◽  
pp. 1-5
Author(s):  
Fatemeh Zovari ◽  
Hadi Parsian ◽  
Ali Bijani ◽  
Ameneh Moslemnezhad ◽  
Atena Shirzad
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Postmenopausal Women ◽  
Total Antioxidant Capacity ◽  
Premenopausal Women ◽  
Control Group ◽  
Antioxidant Power ◽  
Whole Saliva ◽  
Total Antioxidant

Objective. In menopause, reduction of estrogen hormone affects oxidative stress process in serum. Oxidative stress in saliva plays a significant role in the pathogenesis of oral diseases. The aim of this study was to investigate the total antioxidant capacity and lipid peroxidation in the serum and saliva of premenopausal and postmenopausal women. Methods. In this case control study, 50 postmenopausal women (case group) and 48 premenopausal women (control group) were selected. The unstimulated whole saliva and serum of the postmenopausal and premenopausal women were collected. The total antioxidant capacity (TAC) of the saliva and serum was measured by ferric reducing antioxidant power (FRAP). Also, malondialdehyde (MDA) was measured by thiobarbituric acid reactive substance (TBARS) method for serum and saliva. Then, the obtained data were analyzed by SPSS 17, whereby Mann–Whitney test and Spearman’s correlation test were used. P < 0.05 was considered statistically significant. Results. The postmenopausal group had significantly lower mean serum TAC and higher mean serum MDA than the control group ( P < 0 < 001 and P < 0.01 , respectively). The mean salivary TAC and MDA, however, did not differ significantly between the case and control group ( P = 0.64 and P = 0.08 , respectively). Conclusion. In postmenopausal women, with elevation of serum MDA and reduction of serum TAC, the extent of serum oxidative stress grows, but MDA and TAC levels of saliva do not change.

scholarly journals Changes in Bone Metabolism and Antioxidant Defense Systems in Menopause-Induced Rats fed Bran Extract from Dark Purple Rice (Oryza sativa L. Cv. Superjami)

Nutrients ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 2926
Author(s):  
Soo Im Chung ◽  
Su Noh Ryu ◽  
Mi Young Kang
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Bone Loss ◽  
Bone Metabolism ◽  
Rice Bran ◽  
Antioxidant Defense ◽  
Control Group ◽  
Defense Systems ◽  
Antioxidant Defense Systems ◽  
And Oxidative Stress

Menopause is a matter of concern for women’s health due to a deficiency of female hormones; additionally, reactive oxygen species and aging can cause osteoporosis. Food becomes increasingly interesting as a menopausal woman’s alternative to hormone therapy. The effects of ethanol extracts from dark purple Superjami rice bran on bone metabolism and antioxidant defense systems in menopause-induced animal models were evaluated. Female rats underwent sham surgery or were ovariectomized to induce a menopause-like state. Rats were divided into a sham control group (SHAM), an ovariectomized control group (OVX), and an ovariectomized grou supplemented with Superjami rice bran extract group (OVX-S) and fed for 8 weeks. The OVX groups exhibited significantly more weight gain, amounts of bone turnover biochemical markers (alkaline phosphatase, osteocalcin, and C-terminal telopeptide), bone loss, lipid-peroxidation and oxidative stress than the SHAM group. However, Superjami bran extract added to the diet resulted in a significant reduction in body weight and lipid peroxidation, as well as enhanced bone metabolism and antioxidant enzyme activities, in ovariectomized rats. These results propound that extracts from Superjami rice bran have therapeutic potentiality against bone loss and oxidative stress in menopause-induced states and will be useful in preventing postmenopausal osteoporosis and oxidative damage.

Evaluation of glucose-6-phosphate dehydrogenase serum level in patients with multiple sclerosis and neuromyelitis optica

2020 ◽  
Author(s):  
Niloofar Chitsaz ◽  
Leila Dehghani ◽  
Amir Safi ◽  
Nazgol Esmalian-Afyouni ◽  
Vahid Shaygannejad ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Multiple Sclerosis ◽  
Vitamin D ◽  
Serum Level ◽  
Neuromyelitis Optica ◽  
Treatment Plan ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Control Group ◽  
Significant Difference ◽  
Glucose 6 Phosphate Dehydrogenase

Background: Multiple sclerosis (MS) and neuromyelitis optica (NMO) are both demyelinating disorders and oxidative stress is suggested to have a role in their pathogenesis. Glucose-6-phosphate dehydrogenase (G6PD) produces nicotinamide adenine dinucleotide phosphate (NADPH) via the pentose phosphate pathway. NADPH is not only involved in the synthesis of fatty acids necessary for myelination, but also it is involved in the defense against oxidative stress. Prescribing supplementary vitamin D as a part of the MS treatment plan can increase G6PD gene expression. The aim of this study was to determine the serum level of G6PD in patients with MS and NMO and its relationship with vitamin D, since it is yet to be explored thoroughly. Methods: In this case-control study, subjects were divided into three experimental and control groups. The experimental groups comprised 50 patients with relapsing-remitting MS (RRMS) who had a history of vitamin D consumption, 50 newly-diagnosed MS patients, and 50 patients with NMO. Control group included 65 healthy individuals. Serum level of G6PD was measured and compared among these groups. Results: No significant difference was seen between the G6PD level in patients with MS and NMO, but it should be noted that this level was significantly lower than the healthy group. G6PD serum level was significantly higher in patients with MS who had previously consumed supplementary vitamin D compared to those who had not. Conclusion: G6PD deficiency is observed in patients with MS and NMO. Also, supplementary vitamin D may induce favorable results on the G6PD level.

scholarly journals Resveratrol Reverses Thioacetamide-Induced Renal Assault with respect to Oxidative Stress, Renal Function, DNA Damage, and Cytokine Release in Wistar Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Seema Zargar ◽  
Mona Alonazi ◽  
Humaira Rizwana ◽  
Tanveer A. Wani
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Dna Damage ◽  
Creatine Kinase ◽  
Renal Function ◽  
Treated Group ◽  
Control Group ◽  
Cytokine Release ◽  
Kidney Toxicity ◽  
Significant Difference

Background. Thioacetamide (TAA), a class 2B-type carcinogen, is a potent toxicant. Toxicities caused by this compound in various tissues due to oxidative stress, increase of proinflammatory markers, and apoptosis have been reported; however, reports on kidney toxicity are negligible. Resveratrol (RSV), on the other hand, has demonstrated antioxidant and anti-inflammatory effects in different cases. Resveratrol’s protective effects against TAA kidney toxicity were investigated in four rat groups. Methodology. Four groups of rats were studied as follows (n=8): control group, where rats were fed normal diet and water; TAA group, where rats received 0.3% TAA in water for two weeks; RSV group, where rats received 10 mg/kg body weight (bw) of RSV as oral suspension for two weeks; and treated group, where rats orally received 10 mg/kg bw RSV and simultaneously received 0.3% TAA for two weeks. Kidney homogenates from all groups were analyzed for cytokine release (IL-4, TNF-α, and IFN-γ) and oxidative stress (lipid peroxidation, catalase, and 8-OHdG). The serum of rats was analyzed for the quantification of renal function markers (blood urea nitrogen (BUN), creatinine, and creatine kinase). Result. A significant increase in the renal function markers (BUN, 240%; creatinine, 187%; and creatine kinase, 117%), oxidative stress parameters (lipid peroxidation, 192% increase; catalase, 30.5% decrease), cytokines (IL-4, 120%; TNF-α, 129%; and IFN-γ, 133%), and DNA damage was observed in the TAA-treated group. All changes were significantly reversed in the group treated with RSV and TAA (P<0.05) in combination, with no significant difference compared to the control group. Conclusion. We conclude that resveratrol shows protection against TAA toxicity in rat kidney with respect to DNA damage, oxidative stress, renal function and cytokine release.

scholarly journals OXY-SCORE: a new perspective for left ventricular hypertrophy diagnosis

2020 ◽  
Vol 11 ◽  
pp. 204062232093641
Author(s):  
Begoña Quintana-Villamandos ◽  
Laia Pazó-Sayós ◽  
Irene González del Pozo ◽  
Pilar Rodríguez-Rodríguez ◽  
Jose María Bellón ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Left Ventricular Hypertrophy ◽  
Oxidative Damage ◽  
Antioxidant Capacity ◽  
Ventricular Hypertrophy ◽  
Characteristic Curve ◽  
Left Ventricular ◽  
Control Group ◽  
Global Index ◽  
Significant Difference

Background: A recently developed global indicator of oxidative stress (OXY-SCORE), by combining individual plasma biomarkers of oxidative damage and antioxidant capacity, has been validated in several pathologies, but not in left ventricular hypertrophy (LVH). The aim of this study was to design and calculate a plasma oxidative stress global index for patients with LVH. Methods: A total of 70 consecutive adult patients were recruited in our institution and assigned to one of the two study groups (control group/LVH group) by an echocardiography study. We evaluated plasmatic biomarkers of oxidative damage (malondialdehyde and thiolated proteins) and antioxidant defense (total thiols, reduced glutathione, total antioxidant capacity, catalase, and superoxide dismutase activities) by spectrophotometry/fluorimetry in order to calculate a plasma oxidative stress global index (OXY-SCORE) in relation to LVH. Results: The OXY-SCORE exhibited a highly significant difference between the groups ( p < 0.001). The area under the receiver operating characteristic curve was 0.74 (95% confidence interval (CI), 0.62–0.85; p < 0.001). At a cut-off value of −1, the 68.6% sensitivity and 68.6% specificity values suggest that OXY-SCORE could be used to screen for LVH. A multivariable logistic regression model showed a positive association ( p = 0.001) between OXY-SCORE and LVH [odds ratio = 0.55 (95% CI, 0.39–0.79)], independent of gender, age, smoking, glucose, systolic and diastolic arterial pressure, dyslipidemia, estimated glomerular filtration rate, body mass index, and valvular/coronary disease. Conclusion: OXY-SCORE could help in the diagnosis of LVH and could be used to monitor treatment response.

scholarly journals The Differences of Malondialdehyde Serum Level, Expression of Tumor Necrosis Factor Alpha and Vascular Endothelial Growth Factor, and the Area of Endometriotic Implants in Administration of Kebar Grass Extract (Biophytum petersianum) and Green Tea Extract (Camelia sinensis) to Mice

2019 ◽  
Vol 24 (3) ◽  
pp. 169
Author(s):  
Yuli Trisetiyono ◽  
Noor Pramono ◽  
Syarief Thaufik Hidayat ◽  
Widjiati Widjiati
Keyword(s):  
Oxidative Stress ◽  
Serum Level ◽  
Green Tea ◽  
Green Tea Extract ◽  
Control Group ◽  
Vegf Expression ◽  
Necrosis Factor Alpha ◽  
Tnf Α ◽  
Significant Difference ◽  
Tea Extract

The pathological pathway of endometriosis remains unclear and involves complex etiologies. Increased oxidative stress is understood to be related to this disease. Oxidative stress produces reactive oxygen species, causes inflammation that is characterized by recruiting lymphocytes and phagocyte activation, produces cytokines that induce oxidation enzyme, and supports epithelium growth. Oxidative stress conjointly will increase angiogenesis and promotes the proliferation of endometriosis tissue within the peritoneal cavity. Kebar grass and green tea contain high antioxidants, are expected to extend antioxidant defense leading to reduced oxidative stress, inflammation, angiogenesis, and reduced endometriosis tissue implants. The objective is to analyze the consequences of Kebar grass and green tea extract to MDA serum level, TNF-α, and VEGF expression, and the area of the endometriotic implants in the mice models.  The study was an experiment designed. It has been conducted within the Department of Obstetrics Gynecology, Faculty of Medicine Diponegoro University and Faculty of Veterinary Medicine Airlangga University. Twenty-one mice were divided into three groups, i.e., the first group of mouse models was given Kebar extract 3 mg/day; the second group was assigned green tea extract 1.1 mg/day; therefore the third group was a control group contains the untreated endometriosis mice. Each treatment was given for fourteen days. MDA serum level was measured by specto-photometric examination, the area of the endometriotic implants was measured by computer tracing technique, whereas TNF-α and VEGF expression of endometriotic implants were measured by IHC using Rammele Scale Index (ImmunoReactive Score). The MDA serum levels of the groups treated with Kebar grass extract and green tea extract were significantly lower than the control group (0.09±0.022 mmol, 0.07±0.019 mmol, and 0.30±0.062 mmol, respectively; p=0.001). TNF-α expression of the groups supplied with each treatment also lower than the control groups (2.43±1.521, 3.66±1.422, and 7.26±2.898, respectively; p=0.002). However, VEGF expression was not significantly different between Kebar grass extract group, green tea group, and the control (4.34±2.402, 4.57±1.998, 7.40±3.495, respectively; p=0.089). Finally, the area of the endometriotic implants of the mice models administered with all treatment was smaller than the control group (0.01±0.025 mm2, 8.76±18.776 mm2, and 34.80±13.079 mm2, respectively; p=0.003). Conclusion: Kebar grass extract, as well as green tea extract administration to endometriosis model mice, resulted in lower MDA serum level and TNF-α expression, smaller the area of endometriotic implants compared, but not resulted in a significant difference of VEGF expression.

scholarly journals Effects of Thymoquinone on Sperm Parameters, Apoptosis, Testosterone Level, and Oxidative Stress in a Mouse Model of D-Galactose-Induced Aging

2018 ◽  
Vol 24 (3) ◽  
pp. 180-186 ◽  
Author(s):  
Seyedeh Saleheh Attari ◽  
Shabnam Mohammadi ◽  
Alireza Ebrahimzadeh ◽  
Hossein Hosseinzadeh ◽  
Mohammad Soukhtanloo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Mouse Model ◽  
Antioxidant Capacity ◽  
Testosterone Level ◽  
Total Antioxidant Capacity ◽  
Sperm Parameters ◽  
Control Group ◽  
Significant Difference ◽  
Total Antioxidant ◽  
And Oxidative Stress

Background: The aging process is accompanied by low secretion of sex hormones and testicular apoptosis. The antioxidant properties of thymoquinone (TQ) may prevent the effects of aging. Therefore, in the present study, the effects of different doses of TQ were investigated on sperm parameters, testosterone level, apoptosis, and oxidative stress in a mouse model of D-galactose-induced aging. Methods: In this experimental study, 30 adult male mice were randomly divided into 5 groups. The control group did not receive any injections, while the D-galactose group received an intraperitoneal injection of 300 mg/kg of D-galactose for 42 days. The TQ1-TQ3 groups received intraperitoneal injections of 5, 2.5, and 1.25 mg/kg of TQ plus D-galactose, respectively for 14 days (from the 1st to the 14th day of the experiment). The morphometric analysis, testicular apoptosis examination, and sperm analysis were performed, and testosterone level, total antioxidant capacity, and malondialdehyde level were evaluated on day 42 of the experiment. Data were analyzed using SPSS. Results: Administration of TQ in the TQ1 group caused a significant difference in sperm parameters, compared to the D-galactose group (P<0.05). The lowest amount of positive tunnel cells was related to 5 mg/kg of TQ and the highest to 2.5 mg/kg of TQ. There was no significant difference in the parameters of seminal vesicles, epididymis, prostate, and testis between the groups (P>0.05). The malondialdehyde level were decreased in the TQ1-TQ3 groups, compared to the D-galactose group (P<0.001). On the other hand, the total antioxidant capacity was increased significantly in the TQ1 group, compared to the D-galactose group (P<0.001). Conclusion: Administration of 5 mg of TQ for 14 days improved sperm quality and biochemical parameters, while reducing apoptotic cells of the testes in a mouse model of aging.

scholarly journals Resveratrol reduces lipid peroxidation and increases sirtuin 1 expression in adult animals programed by neonatal protein restriction

2010 ◽  
Vol 207 (3) ◽  
pp. 319-328 ◽  
Author(s):  
Juliana Gastão Franco ◽  
Egberto Gaspar de Moura ◽  
Josely Correa Koury ◽  
Paula Affonso Trotta ◽  
Aline Cordeiro ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Normal Diet ◽  
Protein Restriction ◽  
Sirtuin 1 ◽  
Control Group ◽  
Protective Effects ◽  
Sirt1 Expression ◽  
Copper Zinc Superoxide Dismutase ◽  
Significant Difference

Resveratrol (Res) has been associated with protective effects against oxidative stress. This study evaluated the effect of Res over lipid peroxidation, antioxidant defense, hepatic sirtuin 1 (SIRT1), which up-regulates antioxidant enzymes, and copper/zinc superoxide dismutase (Cu/Zn SOD) in adult offspring whose mothers were protein restricted during lactation. Lactating Wistar rats were divided into control (C) group, which were fed a normal diet (23% protein), and low-protein and high-carbohydrate (LPHC) group, which were fed a diet containing 8% protein. After weaning (21 days), C and LPHC offspring were fed a normal diet until they were 180 days old. At the 160th day, animals were separated into four groups as follows: control, control+Res, LPHC, and LPHC+Res. Resveratrol was given for 20 days (30 mg/kg per day by gavage). LPHC animals showed a higher total antioxidant capacity (TAC) without change in lipid peroxidation and SIRT1 expression. The treatment with Res increased TAC only in the control group without effect on lipid peroxidation and SIRT1. LPHC animals treated with Res had lower lipid peroxidation and higher protein and mRNA expression of SIRT1 without any further increase in TAC. No significant difference in liver Cu/Zn SOD expression was observed among the groups. In conclusion, maternal protein restriction during lactation programs the offspring for a higher antioxidant capacity, and these animals seem to respond to Res treatment with a lower lipid peroxidation and higher hepatic SIRT1 expression that we did not observe in the Res-treated controls. It is probable that the protective effect can be attributed to Res activating SIRT1, only in the LPHC-programed group.

Efeitos da Suplementação com Panax ginseng Sobre Parâmetros de Estresse Oxidativo e Lesão Muscular Induzidos por Exercício Exaustivo Agudo em Ratos/Effects of Panax ginseng Supplementation upon Oxidative Stress and Skeletal Muscle Damage Biomarkers Induce

1970 ◽  
Vol 1 (1) ◽  
pp. 59-66
Author(s):  
Lázaro Alessandro Soares Nunes ◽  
Fernando Oliveira Catanho da Silva ◽  
Ana Carolina Ceglio ◽  
Talita de Souza Venturini
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Lipid Peroxidation ◽  
Uric Acid ◽  
Antioxidant Capacity ◽  
Muscle Damage ◽  
Panax Ginseng ◽  
Exhaustive Exercise ◽  
Skeletal Muscle Damage ◽  
Antioxidant Defense Systems

Introdução: O exercício exaustivo agudo aumenta significativamente o consumo de oxigênio pelo organismo, aumentando a produção de Espécies Reativas de Oxigênio (EROs). Por serem moléculas altamente instáveis, as EROs podem causar danos oxidativos e induzir a morte celular. Para minimizar os efeitos deletérios das EROs o organismo possui sistemas de defesa antioxidante. O objetivo deste estudo foi testar o potencial antioxidante do Panax ginseng através da análise de marcadores de peroxidação lipídica, lesão muscular e capacidade antioxidante após um protocolo de exercício exaustivo agudo de natação realizado em ratos. Material e métodos: Foram utilizados 21 ratos Wistar machos divididos em: grupo controle–CO (n=7); grupo suplementado-exercitado–SG (n=7) e grupo não-suplementado-exercitado-NS (n=7). Foram coletados 10 mL de sangue total para obtenção de soro e posterior realização das análises: peroxidação lipídica (MDA), capacidade antioxidante total (CAT), ácido úrico, creatina quinase (CK), aspartato aminotransferase (AST) e alanina aminotransferase (ALT). Resultados: Observou-se maior concentração de MDA no grupo NS (0,60 ± 0,23μM) em relação aos grupos CO (0,18 ± 0,09 μM) e SG (0,26 ± 0,19 μM), p<0,05. Os níveis elevados de AST observados nos grupos SG (141± 24 U/L) e NS (199 ± 92 U/L) em relação ao CO (89 ± 27 U/L), p<0,05, indicam maior nível de lesão muscular nos grupos exercitados. Não houve diferenças estatísticas para ALT, CK, ácido úrico e CAT. Conclusão: A administração crônica de Panax ginseng favorece a proteção contra o excessivo ataque oxidativo muscular, que ocasiona peroxidação lipídica e eventual perda de função celular.Effects of Panax ginseng Supplementation upon Oxidative Stress and Skeletal Muscle Damage Biomarkers Induced by Acute Forced Exercise in RatsIntroduction: Exhaustive exercise increases significantly oxygen consumption by the organism, which leads to increase in Reactive Oxygen Species (ROS) generation. ROS are highly unstable and reactive molecules that can cause oxidative damage and even the cellular death. To minimize ROS deleterious effects, organism has antioxidant defense systems. The aim of this study was to test Panax Panax ginseng antioxidant capacity through lipid peroxidation, oxidative stress and muscle damage biomarkers after a swimming acute exhaustive exercise protocol in Wistar rats. Materials and methods: Twenty-one rats were divided in: control group–CO (n=7) that was not supplemented and did not realize exercise; Panax ginseng supplemented-exercised group–SG (n=7) that did exhaustive exercise and supplementation; not supplemented-exercised group–NS (n=7) that was not supplemented but did exercise. We collected 10mL of blood to obtain serum for biochemical analyzes: lipid peroxidation (MDA), total antioxidant capacity (CAT), uric acid, creatine kinase (CK), aspartate aminotrasferase (AST) and alanine aminotransferase (ALT). Results: There was an increase in MDA concentration in NS group (0.60 ± 0.23 μM) in comparison to CO (0.18 ± 0.09 μM) and SG (0.26 ± 0.19 μM); the higher AST levels on SG (141± 24 U/L) and NS (199 ± 92 U/L) in comparison to CO group (89 ± 27 U/L), indicating higher levels of muscle damage in both exercised groups. There were no statistical differences in CK, uric acid and ALT levels. Conclusion: We concluded that chronic Panax Panax ginseng supplementation can protect organism against increase in lipid peroxidation induced by exhaustive exercise.

Resistance of erythrocytes from Brown trout (Salmo trutta m. trutta L.) affected by ulcerative dermal necrosis syndrome

2011 ◽  
Vol 14 (3) ◽  
pp. 443-448 ◽  
Author(s):  
N. Kurhalyuk ◽  
H. Tkachenko ◽  
K. Pałczyńska
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Brown Trout ◽  
Salmo Trutta ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substance ◽  
Tbars Level ◽  
Reactive Substance ◽  
Brown Trout Salmo Trutta

Resistance of erythrocytes from Brown trout (Salmo trutta m. trutta L.) affected by ulcerative dermal necrosis syndrome In the present work we evaluated the effect of ulcerative dermal necrosis (UDN) syndrome on resistance of erythrocytes to haemolytic agents and lipid peroxidation level in the blood from brown trout (Salmo trutta m. trutta L.). Results showed that lipid peroxidation increased in erythrocytes, as evidenced by high thiobarbituric acid reactive substance (TBARS) levels. Compared to control group, the resistance of erythrocytes to haemolytic agents was significantly lower in UDN-positive fish. Besides, UDN increased the percent of hemolysated erythrocytes subjected to the hydrochloric acid, urea and hydrogen peroxide. Results showed that UDN led to an oxidative stress in erythrocytes able to induce enhanced lipid peroxidation level, as suggested by TBARS level and decrease of erythrocytes resistance to haemolytic agents.

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