scholarly journals Identification of Fungal Pathogens to Control Postharvest Passion Fruit (Passiflora edulis) Decays and Multi-Omics Comparative Pathway Analysis Reveals Purple Is More Resistant to Pathogens than a Yellow Cultivar

2021 ◽  
Vol 7 (10) ◽  
pp. 879
Author(s):  
Hafiz Muhammad Rizwan ◽  
Lin Zhimin ◽  
Wiwiek Harsonowati ◽  
Abdul Waheed ◽  
Yang Qiang ◽  
...  
Keyword(s):  
Metabolic Profiling ◽  
Fungal Pathogens ◽  
Morphological Characteristics ◽  
Storage Period ◽  
Pathogen Resistance ◽  
Passion Fruit ◽  
Colletotrichum Truncatum ◽  
Metabolomics Data ◽  
Passiflora Edulis

Production of passion fruit (Passiflora edulis) is restricted by postharvest decay, which limits the storage period. We isolated, identified, and characterized fungal pathogens causing decay in two passion fruit cultivars during two fruit seasons in China. Morphological characteristics and nucleotide sequences of ITS-rDNA regions identified eighteen isolates, which were pathogenic on yellow and purple fruit. Fusarium kyushuense, Fusarium concentricum, Colletotrichum truncatum, and Alternaria alternata were the most aggressive species. Visible inspections and comparative analysis of the disease incidences demonstrated that wounded and non-wounded yellow fruit were more susceptible to the pathogens than the purple fruit. Purple cultivar showed higher expression levels of defense-related genes through expression and metabolic profiling, as well as significantly higher levels of their biosynthesis pathways. We also found fungi with potential beneficial features for the quality of fruits. Our transcriptomic and metabolomics data provide a basis to identify potential targets to improve the pathogen resistance of the susceptible yellow cultivar. The identified fungi and affected features of the fruit of both cultivars provide important information for the control of pathogens in passion fruit industry and postharvest storage.

scholarly journals Identification and pathogenicity of microorganisms affecting purple passion fruit in Colombia

Revista CERES ◽  
2017 ◽  
Vol 64 (3) ◽  
pp. 250-257
Author(s):  
Joaquín Guillermo Ramírez Gil ◽  
Pablo Julián Tamayo ◽  
Juan Gonzalo Morales
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Morphological Characteristics ◽  
Passion Fruit ◽  
Phytophthora Nicotianae ◽  
Passiflora Edulis ◽  
Fruit Samples ◽  
Phytophthora Drechsleri ◽  
Pathogenicity Tests ◽  
Purple Passion Fruit ◽  
First Time

ABSTRACT Purple passion fruit (Passiflora edulis f. edulis Sims, known in Colombia as “gulupa”) is an increasingly important crop in Colombia, as seen by an increase in the area under cultivation. This recent prominence coincides with a low number of existing technologies related to the cultivation of the fruit, resulting in a lack of knowledge about its associated pathogens and the absence of rapid and precise diagnostic tests. The objective of this study was to determine the pathogenicity of distinct microorganisms isolated from purple passion fruit samples, some of which had not been previously reported in Colombia. The sampling was performed in seven plots located in two regions of the Colombian Antioquia. With the use of field symptomatology, the isolation of microorganisms, morphological characteristics, molecular analysis, and pathogenicity tests, the pathogen species Phytophthora nicotianae var. parasitica, Colletotrichum gloeosporioides sensu lato, and Phytophthora drechsleri were identified for the first time in Colombia. These pathogens cause lesions in fruits, with an incidence of 10.9, 7.6, and 2.8%, respectively. This is also the first report on a global level of Phytophthora drechsleri causing a disease in this plant species.

Effect of an Antimicrobial Packaging on Postharvest Quality of Sweet Cherry

2012 ◽  
Vol 200 ◽  
pp. 249-253
Author(s):  
Xue Mei Yu ◽  
Wen Cai Xu ◽  
Dong Li Li
Keyword(s):  
Fungal Pathogens ◽  
Storage Time ◽  
Sweet Cherry ◽  
Storage Period ◽  
Postharvest Quality ◽  
Postharvest Diseases ◽  
Antimicrobial Packaging ◽  
Sweet Cherries ◽  
Postharvest Treatment

Sweet cherry shows a limited storage time mainly due to incidence of decay and a fast loss of sensory quality of the fruit. Sweet cherries may be damaged seriously by fungal pathogens for a long storage period. For the control of postharvest diseases, the use of chemicals as fungicides has been applied to sweet cherries. In this paper, a kind of antimicrobial packaging, based on our patent , which can release SO2fungicide has been used as postharvest treatment to protect the sweet cherry quality. Cherries quality during storage was determined, and results suggest that SO2 fungicide packaging showed benefits in terms of lower weight loss, lowerSuperscript textdecay rate and maintenance of fruit firmness compared with the control one.

scholarly journals Lipid peroxidation and seed emergency in progenies of the yellow passion fruit plant

2012 ◽  
Vol 34 (3) ◽  
pp. 711-718 ◽  
Author(s):  
João Paulo Bestete de Oliveira ◽  
Rodrigo Sobreira Alexandre ◽  
Jacson Rondinelli da Silva Negreiros ◽  
José Carlos Lopes ◽  
Claudio Horst Bruckner
Keyword(s):  
Lipid Peroxidation ◽  
Experimental Design ◽  
Genetic Variability ◽  
Passion Fruit ◽  
Passiflora Edulis ◽  
Mda Content ◽  
Passiflora Edulis Sims ◽  
Yellow Passion Fruit

The objective was to evaluate the percentage of emergency plantlets and lipid peroxidation in seeds of 29 half-sib progenies of yellow passion fruit (Passiflora edulis Sims.) after 24 months under storage. The experimental design was completely randomized, with four replications of 50 seeds each, from which the treatments were the progenies (1-29). The evaluation of the percent plantlet emergency was accomplished at 14 and 28 days after sowing. The lipid peroxidation of the seeds was expressed as malondialdehyde (MDA) content that was determined by the TBARS method. Approximately 21% of those half-sib progenies maintained the viability of their seeds for twenty-four months under storage. The results point out a remarkable genetic variability for vigor and emergency of the yellow passion fruit plantlets, with occurrence of individuals with high and other ones with low capacity to maintaining the physiologic quality of their seeds after storage.

scholarly journals Use of Salines Waters and Biofertilizers in Quality of the Seedlings From Passiflora edulis Evaluated by the Dry Phytomass

2019 ◽  
Vol 11 (5) ◽  
pp. 121
Author(s):  
Francisco de O. Mesquita II ◽  
Ana C. M. Meireles ◽  
José L. N. Cardoso ◽  
Carlos W. Oliveira ◽  
Patrycia L. B. Roguides
Keyword(s):  
Electrical Conductivity ◽  
Experimental Design ◽  
Irrigation Water ◽  
Passion Fruit ◽  
Passiflora Edulis ◽  
Maximum Capacity ◽  
Saline Waters ◽  
Salinity Increase ◽  
Polyethylene Bags

The objective of this study was to evaluate the effects of the biofertilizers (common and enriched) in substrate irrigated with saline waters on the quality of the seedlings evaluated by the phytomass from yellow Passiflora edullis. In this sense, an experiment was carried in a greenhouse at the Center of Agricultural Sciences and Biodiversity CCAB/UFCA, Crato-CE, from October/2017 to March/2018. The substrate used was a material of the first 20 cm to depth of a Red Yellow Latosol. The experimental design was completely randomized in a factorial scheme of type 5 × 3, with three replications, referring to the electrical conductivity values of the irrigation water: 0.5; 1.0; 2.0; 3.0 and 4.0 dS m-1, in the soil without the bovine biofertilizer; in treatments with common biofertilizer and, evaluated in the soil with enriched biofertilizer, conditioned in black polyethylene bags with it a maximum capacity of 5.0 kg, totaling 60 treatments. The fermented bovine biofertilizer after dilution in non-saline and non-chlorinated water (0.5 dS m-1), in the proportion of 1:3, was applied only once to 10% of the substrate volume, two days before sowing. The salinity increase of the irrigation water affected negatively the phytomass production, but with less intensity in the treatments with enriched biofertilizer. The enriched and common biofertilizer provided higher growth and consequently higher phytomass production of passion fruit seedlings in relation to the soil without the respective input, independently of the level of salinity of irrigation water.

scholarly journals MORFOFISIOLOGIA E QUALIDADE DE MUDAS DE MARACUJAZEIRO SOB DIFERENTES NATUREZAS CATIÔNICAS DA ÁGUA E H2O2

Irriga ◽  
2021 ◽  
Vol 1 (1) ◽  
pp. 184-205
Author(s):  
José Joedson Lima Silva ◽  
Geovani Soares De Lima ◽  
Lauriane Almeida dos Anjos Soares ◽  
Iara Almeida Roque ◽  
Maíla Vieira Dantas ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Salt Stress ◽  
Agricultural Sector ◽  
Block Design ◽  
Passion Fruit ◽  
Water Salinity ◽  
Stress Effect ◽  
Passiflora Edulis ◽  
E Mail

MORFOFISIOLOGIA E QUALIDADE DE MUDAS DE MARACUJAZEIRO SOB DIFERENTES NATUREZAS CATIÔNICAS DA ÁGUA E H2O2     JOSÉ JOEDSON LIMA SILVA1; GEOVANI SOARES DE LIMA2; LAURIANE ALMEIDA DOS ANJOS SOARES1; IARA ALMEIDA ROQUE1, MAÍLA VIEIRA DANTAS2 E LUDERLÂNDIO DE ANDRADE SILVA2   1 Unidade Acadêmica de Ciências Agrárias, Universidade Federal de Campina Grande, UFCG, Rua Jario Vieira Feitosa, 1770, Bairro dos Pereiros, Pombal, PB, Brasil. E-mail: [email protected]; [email protected]; [email protected]. 2 Unidade Acadêmica de Engenharia Agrícola, Universidade Federal de Campina Grande, UFCG, Rua Aprígio Veloso, 882 - Universitário, Campina Grande, PB, Brasil. E-mail: [email protected]; [email protected].; [email protected].     1 RESUMO   No decorrer dos anos, a salinidade das águas se tornou um dos principais obstáculos do setor agrícola. Este trabalho objetivou avaliar o crescimento, as relações hídricas e a qualidade de mudas de maracujazeiro irrigadas com águas de diferentes composições catiônicas e peróxido de hidrogênio. O experimento foi conduzido em condição de casa de vegetação, no munícipio de Pombal - PB, utilizando-se o delineamento em blocos casualizados, com fatorial 6 x 4, sendo seis composições catiônicas da água de irrigação [S1 – Testemunha (0,3 dS m-1); S2 - Na+; S3 - Ca2+; S4 - Na+ + Ca2+; S5 - Mg2+ e S6 - Na+ + Ca2+ + Mg2+) e quatro concentrações de peróxido de hidrogênio – H2O2 (0, 20, 40 e 60 μM), com quatro repetições. Com exceção do S1, os demais tratamentos foram irrigados com água de 3,6 dS m-1. A concentração de 40 µM de H2O2 reduziu o efeito do estresse salino na área foliar das plantas irrigadas com águas constituídas de Na+, Na+ + Ca2+ e Na+ + Ca2+ + Mg2+. As concentrações de 20 e 40 µM de H2O2 elevaram o índice de qualidade de Dickson das plantas irrigadas com água constituída de Na+ + Ca2+ + Mg2+.   Palavras-chave: Passiflora edulis f. flavicarpa, salt stress, peróxido de hidrogênio.     SILVA, J. J. L.; LIMA, G. S. de; SOARES, L. A. dos A.; ROQUE, I. A.; DANTAS, M. V.; SILVA, L. de A. MORPHOPHYSIOLOGY AND QUALITY OF PASSION FRUIT SEEDLINGS UNDER DIFFERENT CATIONIC NATURES OF WATER AND H2O2     2 ABSTRACT   Over the years, water salinity has become one of the main obstacles in the agricultural sector. This study aimed to evaluate the growth and quality of passion fruit cv. BRS GA1 as a function of different cationic nature of the water and exogenous application of hydrogen peroxide. The experiment was conducted in greenhouse conditions at the Center of Agrifood Science and Technology of the Federal University of Campina Grande, Pombal, Brazil, using a randomized block design, with a 6 x 4 factorial, corresponding to six combinations of water salinity [S1 – Control (0,3 dS m-1); S2 - Na+; S3 - Ca2+; S4 - Na+ + Ca2+; S5 - Mg2+ and S6 - Na+ + Ca2+ + Mg2+] and four concentrations of hydrogen peroxide - H2O2 (0, 20, 40 and 60 μM), with four repetitions. With the exception of S1, the other treatments were irrigated with water of 3.6 dS m-1. The 40 µM concentration of H2O2 reduced the salt stress effect on leaf area of plants irrigated with water consisting of Na+, Na+ + Ca2+ and Na+ + Ca2+ + Mg2+. The 20 and 40 µM concentrations of H2O2 increased the Dickson quality index of plants irrigated with water consisting of Na+ + Ca2+ + Mg2+.   Keywords: Passiflora edulis f. flavicarpa Degener, salt stress, hydrogen peroxide.

scholarly journals First report of Septoria blotch of passion fruit caused by Septoria passifloricola in Taiwan

Plant Disease ◽  
2020 ◽  
Author(s):  
Yu-Lun Dai ◽  
Ching-Chung Wang ◽  
Huey-Ling Lin ◽  
Chih-Li Wang
Keyword(s):  
South America ◽  
Morphological Characteristics ◽  
Passion Fruit ◽  
High Humidity ◽  
Conidial Suspension ◽  
Tubulin Gene ◽  
Passiflora Edulis ◽  
Leaf Disease ◽  
The Impact ◽  
Β Tubulin

Passion fruit originated in South America and cultivated in tropical and subtropical countries for the fresh market and juice processing. In Taiwan, healthy grafted seedlings of passion fruit have been used for replanting every year to minimize the impact of viral and root diseases. The grafted seedlings commonly used purple passion fruit ‘Tainung NO.1’ (Passiflora edulis × Passiflora edulis forma flavicarpa) abbreviated as PPF as scion, and yellow passion fruit (P. edulis f. flavicarpa) abbreviated as YPF as rootstock. In July 2016 and May 2018, a new leaf disease of passion fruit was observed in Taichung City and Nantou County on 2 to 3-month-old grafted passion fruit seedlings. About 17% of seedlings showed symptoms on leaves in a commercial greenhouse nursery. The infected leaves abscised earlier, causing reduced survival of grafted seedlings. The leaf lesions on YPF and PPF were round to irregular and white-grayish or light brown, and were surrounded by dark green borders and obvious chlorotic halos. Fungal pycnidia were formed in the center of lesions, and extruded yellow-white long conidial tendrils under high humidity. The presumed fungal pathogens were obtained by single spore isolation. Six isolates from the two geographic regions with similar morphological characteristics on potato dextrose agar were obtained. To confirm the pathogenicity, YPF seedlings were inoculated by dropping 10 μL of a conidial suspension of isolate PLS-S2 (107 conidia/mL) on each inoculation site located on abaxial leaves surfaces that were either intact or wounded to form 3 pinpricks in a 4 mm area with a sterilized needle. Three plants were used in a treatment and four leaves of each plant were inoculated. The inoculated plants were kept in plastic bags with high humidity for 3 days and grown in a walk-in growth chamber at 24℃ with a 12-h light regime. The initial symptoms were punctate lesions that later enlarged to round, necrotic spots surrounded by yellow halos, which resembled symptoms in commercial greenhouse nurseries. About 44% of inoculation sites (n= 48) on intact leaves developed lesions at 28 days post-inoculation (dpi) while 100% of inoculation sites (n= 72) on wounded leaves showed lesions at 21 dpi. No lesions developed on leaves with water control. Pathogens reisolated from these lesions were morphologically identical to the inoculated fungus. Conidia were hyaline, filiform to cylindrical with 1-3 nonconstricted septa, and mostly 9-30 × 1.0-2.3 μm. The morphological characteristics of the isolates were similar to Septoria passifloricola Punith (Cline, 2006). Molecular identification was based on concatenated sequences of partial TEF1-α gene (accession nos. MK643056 to MK643061) and β-tubulin gene (accession nos. MK643050 to MK643055) for each of the six isolates. The BLAST search revealed that strain PLS-S2 was 100.0% identical (392 bp) to S. passifloricola CBS 129431 for the TEF1-α gene (KF253443.1) and 98.4% identical (311 bp) for the β-tubulin gene (KF252964.1). Phylogenetic analysis showed that PLS-S2 and five additional isolates clustered with reference strains of S. passifloricola (Verkley et al. 2013) in a well-supported clade (95% bootstrap value). Results suggested that the leaf disease of passion fruit in Taiwan was caused by S. passifloricola. This disease has been reported in Africa, India, Australia, New Zealand, Caribbean, and South America (Cline 2006; Ploetz et al. 2003). If appropriate control actions are not taken, the disease may become a major leaf disease in nurseries in Taiwan.

scholarly journals ÁGUA SALINA E BIOFERTILIZANTE DE ESTERCO BOVINO NA FORMAÇÃO E QUALIDADE DE MUDAS DE MARACUJAZEIRO AMARELO

Irriga ◽  
2016 ◽  
Vol 21 (4) ◽  
pp. 779-795 ◽  
Author(s):  
Sherly Aparecida da Silva Medeiros ◽  
Lourival Ferreira Cavalcante ◽  
Marlene Alexandrina Ferreira Bezerra ◽  
José Adeilson Medeiros do Nascimento ◽  
Francisco Thiago Coelho Bezerra ◽  
...  
Keyword(s):  
Irrigation Water ◽  
Dry Mass ◽  
Cattle Manure ◽  
Passion Fruit ◽  
Water Salinity ◽  
Organic Input ◽  
Passiflora Edulis ◽  
Irrigation Water Salinity ◽  
Yellow Passion Fruit

ÁGUA SALINA E BIOFERTILIZANTE DE ESTERCO BOVINO NA FORMAÇÃO E QUALIDADE DE MUDAS DE MARACUJAZEIRO AMARELO SHERLY APARECIDA DA SILVA MEDEIROS1; LOURIVAL FERREIRA CAVALCANTE2; MARLENE ALEXANDRINA FERREIRA BEZERRA1; JOSÉ ADEILSON MEDEIROS DO NASCIMENTO3; FRANCISCO THIAGO COELHO BEZERRA4 E STELLA DA SILVA PRAZERES5 1Doutoranda PPGA/CCA/UFPB, email: [email protected];  [email protected] do PPGA/CCA/UFPB e Pesquisador do INCTSal, Fortaleza, CE. E-mail: [email protected] Professor Dr. IFCE/ Tianguá - CE. Email: [email protected] do PPGA/CCA/UFPB, Areia-PB. Email: bezerra­‑[email protected] Doutoranda do PPGCS/CCA/UFPB, Areia-PB. Email: stella­[email protected]  1 RESUMO No período de janeiro a março de 2013, um experimento foi conduzido, em estufa telada do Centro de Ciências Agrárias, Universidade Federal da Paraíba, Areia-PB, Brasil, para avaliar os efeitos da salinidade da água de irrigação e esterco líquido fermentado de bovino na formação e qualidade de mudas de maracujazeiro amarelo. O delineamento experimental foi em blocos casualizados, adotando o esquema fatorial 2 x 5 x 2, referente a dois genótipos de maracujazeiro amarelo (genótipo local tradicionalmente cultivado na cidade de Nova Floresta Paraíba, conhecido por Guinezinho e o genótipo BRS Gigante Amarelo), cinco níveis de salinidade da água de irrigação de 0,3; 1,0; 2,0; 3,0 e 4,0 dS m-1, no solo sem e com esterco líquido fermentado de bovino. As variáveis analisadas foram índice de velocidade de emergência, emergência, altura, diâmetro caulinar, área foliar, massa seca da raiz e da parte aérea das mudas e índice de qualidade de Dickson. O biofertilizante líquido de bovino proporcionou a formação de mudas de qualidade adequada ao plantio, em ambos os genótipos de maracujazeiro amarelo, comparadas às obtidas no solo sem o respectivo insumo, independentemente do nível de salinidade das águas de irrigação. Palavras-chave: Passiflora edulis, estresse salino, insumo orgânico.  MEDEIROS, S.A.S; CAVALCANTE, L.F.; BEZERRA, M.A.F.; NASCIMENTO, J.A. M.; BEZERRA, F.T.C; PRAZERES, S.S.SALINE WATER AND BOVINE MANURE BIOFERTILIZER IN THE FORMATION AND QUALITY OF YELLOW PASSION FRUIT SEEDLINGS   2 ABSTRACT                                              During the period of January to March 2013, an experiment was carried out in greenhouse conditions at the Agrarian Sciences Centre, Federal University of Paraíba, Areia municipality, Paraíba State, Brazil, in order to evaluate the effects of irrigation water salinity and liquid fermented cattle manure during formation and quality of yellow passion fruit seedlings. The experimental design was in randomized blocks adopting a factorial design 2 x 5 x 2 referring to two genotypes of yellow passion fruit (Local genotype known as Guinezinho and BRS Yellow Giant genotype), five levels of water salinity irrigation of 0.3; 1.0; 2.0; 3.0 and 4.0 dS m-1 in soil with and without fermented liquid cattle manure. The variables analyzed were emergency speed index, emergency, plants height, stalk diameter, root dry mass and shoot, leaf area, and Dickson quality index. The liquid cattle biofertilizer provided the formation of seedlings with suitable quality for plantation, in both  passion fruit genotypes compared to those obtained on the substrate without biofertilizer, regardless of the salinity of irrigation water. Keywords: Passiflora edulis, salt stress, organic input.

scholarly journals First Report of Collar Rot in Purple Passion Fruit (Passiflora edulis) Caused by Neocosmospora solani in Yunnan province, China

Plant Disease ◽  
2021 ◽  
Author(s):  
Chun-Hui Hao ◽  
Xia Chai ◽  
Fu-Chuan Wu ◽  
Zeng-Fu Xu
Keyword(s):  
Fusarium Solani ◽  
Yunnan Province ◽  
Morphological Characteristics ◽  
Passion Fruit ◽  
Disease Spread ◽  
Causal Organism ◽  
Passiflora Edulis ◽  
Tropical Fruit ◽  
Collar Rot ◽  
Purple Passion Fruit

Purple passion fruit (Passiflora edulis Sims) is a perennial climbing vine native to South America that is grown worldwide as an edible tropical fruit with excellent nutritional value and high economic value (Zibadi et al. 2007). With the increasing expansion of the plantation area in China, considerable economic loss caused by collar rot has attracted wide attention. From 2018-2020, collar rot resulted in the death of many plants of P. edulis 'Mantianxing', a commercial cultivar in China, in southwest China's Yunnan province. The disease spread quickly, and field incidence reached more than 50%. Stem rot symptoms were observed at the base of the stem, about 5-10 cm from the ground, resulting in wilting, defoliation, and death of plants. Representative symptomatic samples were collected from the base of five plants, surface disinfested for 30 seconds with 75% ethanol and 15 min with 10% hypochlorite, washed three times with sterile distilled water, then transferred to potato dextrose agar (PDA) dishes. After 2 days in the dark at 28℃, emerging fungal colonies were purified on new PDA dishes cultured at 28℃ for 7 days. The mycelia were flocculent. The color of the surface and the reverse colony was white and cream, respectively. On synthetic nutrient agar (SNA) medium, microconidia were oval, ellipsoidal or reniform, 0- or 1-septate, and 6.7-23.1 μm in length (n>30); macroconidia were straight to slightly curved, 3- or 5-septate, and 30.8-53.9 μm in length (n>30). Genomic DNA, extracted from six isolates, was amplified with three pairs of primers, ITS1 and ITS4 (White et al. 1990) , EF1-728F and EF1-986R (Carbone and Kohn 1999), and fRPB2-5F and fRPB2-7cR (Liu et al. 1999). The amplicons from all six isolates were sequenced and identical sequences obtained. The sequence of one representative isolate was uploaded to NCBI (National Center for Biotechnology Information) and analyzed with BLASTn in the Fusarium MLST database (https://fusarium.mycobank.org). The sequence of the internal transcribed spacer 1 (ITS1) region (GenBank MN944550) showed 99.1% (449/453 bp) identity to Fusarium solani strain NRRL 53667 (syn: Neocosmospora solani, GenBank MH582405). The sequence of the translation elongation factor-1 (EF-1) gene (GenBank MN938933) showed 97.8% identity (263/269 bp) to F. solani strain NRRL 32828 (GenBank DQ247135). The sequence of the second largest subunit of RNA polymerase Ⅱ (RPB2) gene (GenBank MW002686) showed 98.7% identity (810/821 bp) to F. solani strain NRRL 43441 (GenBank MH582407). Based on a multilocus phylogenetic analysis of the ITS1, EF-1 and RPB2 sequences, coupled with the morphological characteristics, the isolate (designated as NsPed1) was considered to be Neocosmospora solani (syn: Fusarium solani) (Crespo et al. 2019). Subsequently, three-month-old healthy seedlings and 45-day-old cuttings of P. edulis 'Mantianxing' plants were inoculated with the isolate NsPed1 to test its pathogenicity. Stems were wounded, approximately 1-2 mm deep, in the collar region of plants at 2 cm above the soil. A disk (9 mm in diameter) of NsPed1-colonized PDA was placed on the wound. Sterile PDA served as controls. All plants were kept in a growth chamber with 28-30°C, 60% relative humidity, and 16/8-h light/dark photoperiod. Fifteen plants were used for each treatment and replicated three times. Two weeks after inoculation, the stems of the inoculated plants turned brown with a lesion, 2-5 cm in length, and the leaves wilted. These symptoms were similar to those of the diseased plants in the field. The control plants were asymptomatic. N. solani NsPed1 was re-isolated from the infected plants, satisfying Koch’s postulates. Taken together, N. solani NsPed1 was identified as the causal pathogen of collar rot in P. edulis 'Mantianxing'. Knowledge of the causal organism of collar rot in purple passion fruit will lead to improved measures to prevent and control the disease in China and other countries.

scholarly journals First report of Colletotrichum tropicale causing anthracnose on Passiflora edulis in Brazil

Plant Disease ◽  
2021 ◽  
Author(s):  
Jackeline Laurentino da Silva ◽  
Walisson Ferreira da Silva da Silva ◽  
Luiz Eduardo Monteiro Lopes ◽  
Maria Jussara dos Santos Silva ◽  
Janaíne Rossane Araújo Silva-Cabral ◽  
...  
Keyword(s):  
Filter Paper ◽  
Morphological Characteristics ◽  
Oxygen Demand ◽  
Negative Control ◽  
Passion Fruit ◽  
Northeastern Brazil ◽  
Distilled Water ◽  
Passiflora Edulis ◽  
First Report ◽  
Colletotrichum Tropicale

Brazil is the world's largest producer and consumer of yellow passion fruit (Passiflora edulis f. flavicarpa), mainly for the manufacture of concentrate and frozen juice as well as for fresh consumption (Faleiro et al. 2005). Between June and July 2018, passion fruit plants with symptoms of anthracnose were observed in commercial planting in the municipality of Coruripe (20 ha), northeastern state of Alagoas, Brazil. Approximately 70% of the plants showed leaves with relatively large, watery, circular spots that affected 30% of the leaf surface. Small fragments taken from the transition region of symptomatic tissue were superficially disinfected in 70% ethanol for 30 s and in 1% NaClO for 1 min, rinsed in sterile distilled water (SDW), dried on filter paper, plated on potato dextrose agar (PDA-Kasvi) incubated at 25°C under white light and 12 h photoperiod, for 3 days. Two isolates were obtained and deposited in the Collection of Phytopathogens at the Universidade Federal de Alagoas (COUFAL0281 and COUFAL0282). To identify the isolates, partial sequences of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and β-tubulin (TUB2) genes and of the rDNA-ITS (ITS) region were amplified by PCR. The partial sequences were deposited at GenBank (MT299339, MT334694, MT310553, MT299340, MT334695 and MT310554). Based on the BLASTn analysis, sequences of the PCR products showed high nucleotide similarity with sequences of the species C. tropicale (CBS 124949/ex-type and ICMP 18672), for GAPDH (98.94% and 100%), TUB2 (99 and 100%) genes and ITS (100%). This result was also confirmed in the phylogenetic tree of Bayesian Inference assembled with concatenated data (GAPDH, TUB2 and ITS). The colonies of the isolates were white with a white reverse, with dense mycelium, and mean growth rate of 7.54 mm/day, after 7 days on PDA medium at 25° C. Conidia were subcylindrical with rounded ends, hyaline, smooth walls and measured 13.63-20.59μm (= 17.54μm; n= 50) in length and 4.40-7.93 μm (= 5.88 μm; n= 50) in width. Appressoria were melanized, subglobose, irregular and measured 7.44 - 18.57 μm (= 10.04 μm; n= 50) in length and 5.49-10.16 μm (= 7.66 μm; n= 50) in width. These morphological characteristics were consistent with those described for Colletotrichum tropicale E.I. Rojas, S.A. Rehner & Samuels (Rojas et al. 2010). To confirm pathogenicity, 30 µL of a 106 conidia/mL sterile distilled water (SDW) conidia suspension, together with a drop of 20% Tween were deposited on the adaxial surface of passion fruit leaves wounded with a sterile needle, with four repetitions. The control consisted of leaves inoculated only with SDW. The leaves were placed in a plastic Gerbox box with sterilized filter paper moistened with SDW and maintained in a Biochemistry Oxygen Demand (BOD) incubator stove at 25 ºC and photoperiod of 12 h. After 7 days, typical anthracnose symptoms were observed on inoculated leaves. The pathogen was re-isolated and confirmed by morphological characterization, according to Koch's postulates. No symptoms were observed in the negative control. The occurrence of this species has been frequently reported in several other crops grown in northeastern Brazil (Silva et al. 2017; Veloso et al. 2018; Vieira et al. 2018; Costa et al. 2019). Additionally, many of these crops are grown in close proximity to the passion fruit orchards, thus favoring pathogen movement between hosts, probably, due to the anthropic influence, circulation of animals and insects, as well as wind driven rain splashes. However, this is first report of C. tropicale in Passiflora edulis in the world.

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