scholarly journals Illustrating and Enhancing the Biosynthesis of Astaxanthin and Docosahexaenoic Acid in Aurantiochytrium sp. SK4

Marine Drugs ◽  
2019 ◽  
Vol 17 (1) ◽  
pp. 45 ◽  
Author(s):  
Jingrun Ye ◽  
Mengmeng Liu ◽  
Mingxia He ◽  
Ying Ye ◽  
Junchao Huang
Keyword(s):  
Fatty Acids ◽  
Docosahexaenoic Acid ◽  
Polyketide Synthase ◽  
Docosapentaenoic Acid ◽  
Total Carotenoids ◽  
Geranyl Diphosphate ◽  
Geranyl Diphosphate Synthase ◽  
Total Fatty Acids ◽  
Promising Source ◽  
Β Carotene

The marine thraustochytrids are a promising source of docosahexaenoic acid (DHA) and the ketocarotenoid astaxanthin. In this study, the biosynthetic pathways of these two important metabolites in Aurantiochytrium sp. SK4 was illustrated by the analyses of the genome, transcriptome, key enzymes, and pathway products. Two sets of genes were involved in two pathways for the biosynthesis of fatty acids. The absence of Δ-15 desaturase genes and the presence of docosapentaenoic acid (DPA), up to 12% of total fatty acids suggest that Aurantiochytrium sp. SK4 may synthesize DHA mainly via a polyketide synthase (PKS) pathway. Three enzymes, namely geranyl diphosphate synthase (GPPS), farnysyl diphosphate synthase (FPPS), and geranylgeranyle diphosphate synthase (GGPPS) were found to be involved in the formation of GGPP that was subsequently catalyzed to β-carotene by a trifunctional CrtIBY enzyme. β-Carotene might be ketolated and then hydroxylated into astaxanthin based on the carotenoid profiles. The formation of GGPP was proposed to be the limiting steps for carotenoid production. Overexpression of the Archaeoglobus GPS together with the Escherichia coli isopentenyl pyrophosphate isomerase, and Vitreoscilla hemoglobin resulted in not only 1.85- and 5.02-fold increases of total carotenoids and astaxanthin, but also 2.40- and 2.74-fold increases of total fatty acids and DHA. This study provides insights into the biosynthesis of carotenoids and fatty acids in Aurantiochytrium.

scholarly journals Chlamydomonas reinhardtii Is a Potential Food Supplement with the Capacity to Outperform Chlorella and Spirulina

2020 ◽  
Vol 10 (19) ◽  
pp. 6736
Author(s):  
Randa Darwish ◽  
Mohamed A. Gedi ◽  
Patchaniya Akepach ◽  
Hirut Assaye ◽  
Abdelrahman S. Zaky ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Chlamydomonas Reinhardtii ◽  
Unsaturated Fatty Acids ◽  
Model Organism ◽  
Dry Weight ◽  
Essential Amino Acids ◽  
Food Supplement ◽  
Alpha Linolenic Acid ◽  
Total Carotenoids ◽  
Total Fatty Acids

Chlamydomonas reinhardtii is a green microalgae used as a model organism associated with biotechnological applications, yet its nutritional value has not been assessed. This study investigates the nutritional capacity of C. reinhardtii as an additional value for this species beyond its known potential in biofuels and bio-products production. The composition of key nutrients in C. reinhardtii was compared with Chlorella and Spirulina, the species widely regarded as a superfood. The results revealed that the protein content of C. reinhardtii (46.9%) was comparable with that of Chlorella (45.3) and Spirulina (50.4%) on a dry weight basis. C. reinhardtii contained all the essential amino acids with good scores based on FAO/WHO values (0.9–1.9) as in Chlorella and Spirulina. Unsaturated fatty acids predominated the total fatty acids profile of C. reinhardtii were ~74 of which ~48% are n-3 fatty acids. Alpha-linolenic acid (ALA) content in C. reinhardtii (42.4%) was significantly higher than that of Chlorella (23.4) and Spirulina (0.12%). For minerals, Spirulina was rich in iron (3.73 mg/g DW) followed by Chlorella (1.34 mg/g DW) and C. reinhardtii (0.96 mg/g DW). C. reinhardtii, unlike the other two species, consisted of selenium (10 µg/g DW), and had a remarkably lower heavy metal load. Moreover, C. reinhardtii contained relatively high concentrations of chlorophyll (a + b) and total carotenoids (28.6 mg/g DW and 6.9 mg/g DW, respectively) compared with Chlorella (12.0 mg/g DW and 1.8 mg/g DW, respectively) and Spirulina (8.6 mg/g DW and 0.8 mg/g DW, respectively). This study confirms that, based on its nutrient credentials, C. reinhardtii has great potential as a new superfood or ingredient for a food supplement.

scholarly journals Fish Oil–Derived Fatty Acids, Docosahexaenoic Acid and Docosapentaenoic Acid, and the Risk of Acute Coronary Events

Circulation ◽  
2000 ◽  
Vol 102 (22) ◽  
pp. 2677-2679 ◽  
Author(s):  
Tiina Rissanen ◽  
Sari Voutilainen ◽  
Kristiina Nyyssönen ◽  
Timo A. Lakka ◽  
Jukka T. Salonen
Keyword(s):  
Fatty Acids ◽  
Docosahexaenoic Acid ◽  
Fish Oil ◽  
Docosapentaenoic Acid ◽  
Coronary Events

scholarly journals Nutrient Profiles of Wild and Captive Attwater's and Greater Prairie-Chicken Eggs

2018 ◽  
Vol 10 (1) ◽  
pp. 38-50
Author(s):  
Michael E. Morrow ◽  
Elizabeth A. Koutsos ◽  
John E. Toepfer
Keyword(s):  
Fatty Acids ◽  
Maternal Nutrition ◽  
Wild Populations ◽  
Total Carotenoids ◽  
Offspring Fitness ◽  
Prairie Chicken ◽  
Greater Prairie Chicken ◽  
Nutrient Profiles ◽  
Chicken Eggs ◽  
Β Carotene

Abstract We determined reference levels of minerals, fatty acids, and fat-soluble micronutrients in eggs from wild Attwater's Tympanuchus cupido attwateri and two (Minnesota, Nebraska) greater prairie-chicken T. c. pinnatus populations for comparison with eggs produced by captive Attwater's prairie-chickens to help guide formulation and evaluation of captive diets. Levels of all minerals found in wild Attwater's prairie-chicken eggs were similar to those in at least one of the two greater prairie-chicken populations, but these levels frequently differed between the two greater prairie-chicken populations. Ratios for n-6:n-3 fatty acids were >3 times higher for Minnesota greater prairie-chickens, which had more access to waste grain than Attwater's or Nebraska greater prairie-chickens. Captive eggs had n-6:n-3 ratios 6.7 times the pooled wild samples, while wild eggs had higher levels of anhydrolutein, zeaxanthin, β-carotene, and total carotenoids. More magnesium, zinc, and manganese were observed in wild eggs compared with those produced in captivity. Flaxseed was added to the captive breeder diet in an attempt to lower egg n-6:n-3 ratios, along with additional carotenoids found in marigold extract. These dietary modifications successfully lowered the n-6:n-3 ratio by 46%, but this ratio was still 3.6 times higher in captive eggs, consistent with the grain-based formulation of the breeder diet. Carotenoid additions successfully raised total carotenoids, but increases were primarily for lutein and not zeaxanthin or β-carotene as intended. Variability in egg nutrient composition among the three wild populations suggests that some tolerance exists in maternal diets, but impacts to offspring fitness are unknown. Given the purported importance of maternal nutrition to fitness of embryos and neonate chicks, we suggest additional research is needed to quantify the influence of key nutrient levels on offspring fitness for both captive and wild populations.

scholarly journals Development of Growth Media from Agricultural By-Products for Cultivation of PUFA-Producing Sicyoidochytrium minutum

Marine Drugs ◽  
2021 ◽  
Vol 20 (1) ◽  
pp. 8
Author(s):  
Heiðrún Eiríksdóttir ◽  
Magnús Örn Stefánsson ◽  
Hjörleifur Einarsson
Keyword(s):  
Fatty Acids ◽  
Carbon Source ◽  
Docosahexaenoic Acid ◽  
Basal Medium ◽  
Dry Weight ◽  
Growth Media ◽  
Marine Oils ◽  
Human Diet ◽  
Total Fatty Acids ◽  
By Products

The demand for novel sources of marine oils, which contain polyunsaturated fatty acids (PUFAs), has increased due to the realization of the importance of PUFAs, e.g., docosahexaenoic acid (DHA), in the human diet. However, the natural supply is limited. By-product peptones (BYPP) intended as a growth medium for the PUFA-producing strain Sicyoidochytrium minutum of family Thraustochytriaceae were produced after several experiments on the pancreatic digestion of bovine lungs and spleens. S. minutum was able to grow in a medium containing BYPP made from the pancreatic digestion of lung and spleen with glycerol, resulting in 1.14 ± 0.03 g cell dry weight (CDW)/L and 1.44 ± 0.24 g CDW/L, respectively, after 5 days of incubation at 25 °C, compared to 1.92 ± 0.25 g CDW/L in Basal Medium (BM) containing tryptone, peptone, and glycerol. The lipid content, obtained after growth in lung BYPP media with glycerol as a carbon source, was significantly higher (28.17% ± 1.33 of dry weight) than in the control basal medium (BM) (21.72% ± 2.45); however, DHA as a percentage of total fatty acids was lower in BYPP than in the control BM (25.24% ± 1.56 and 33.02% ± 2.37, respectively). It is concluded that low-value by-products from abattoirs can be used as ingredients for the cultivation of oligogenic Thraustochytriaceae.

scholarly journals Quality characteristics and thermal behavior of buriti (Mauritia flexuosa L.) oil

2018 ◽  
Vol 68 (4) ◽  
pp. 220 ◽  
Author(s):  
M. L.F. Freitas ◽  
R. C. Chisté ◽  
T. C. Polachini ◽  
L. A.C.Z. Sardella ◽  
C. P.M. Aranha ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Thermal Behavior ◽  
Monounsaturated Fatty Acids ◽  
Complete Characterization ◽  
Total Carotenoids ◽  
Electronic Density ◽  
Mauritia Flexuosa ◽  
Buriti Oil ◽  
Β Carotene

This work reports a complete characterization of buriti oil. Physicochemical properties were determined according to AOCS methodologies and thermophysical properties were measured using a controlled stress rheometer and a digital electronic density meter. β-carotene and tocopherol contents were obtained using HPLC systems. Fatty acids and acylglycerol classes were determined using GC and HPSEC systems, respectively, while triacylglycerol composition was estimated using the software PrOleos. Thermal behavior (crystallization and melting) was analyzed using a DSC. The results attested high levels of total carotenoids with β-carotene as the major one; total tocopherols contained α- and β-tocopherols which accounted for 91% of the total; and monounsaturated fatty acids were mainly represented by oleic acid. The results showed close agreement between density and viscosity of buriti and olive oils. The crystallization and melting peaks occurred at -43.06 °C and -2.73 °C, respectively. These properties enable Buriti oil to be recommended as an excellent alternative for enriching foods with bioactive compounds.

scholarly journals Polyunsaturated fatty acids and risk of Alzheimer’s disease: a Mendelian randomization study

2019 ◽  
Vol 59 (4) ◽  
pp. 1763-1766 ◽  
Author(s):  
Yasutake Tomata ◽  
Susanna C. Larsson ◽  
Sara Hägg
Keyword(s):  
Fatty Acids ◽  
Arachidonic Acid ◽  
Linoleic Acid ◽  
Docosahexaenoic Acid ◽  
Polyunsaturated Fatty Acids ◽  
Eicosapentaenoic Acid ◽  
Linolenic Acid ◽  
Mendelian Randomization ◽  
Docosapentaenoic Acid ◽  
Alpha Linolenic Acid

Abstract Purpose Observational studies have suggested that polyunsaturated fatty acids (PUFAs) may decrease Alzheimer’s disease (AD) risk. In the present study, we examined this hypothesis using a Mendelian randomization analysis. Methods We used summary statistics data for single-nucleotide polymorphisms associated with plasma levels of n-6 PUFAs (linoleic acid, arachidonic acid) and n-3 PUFAs (alpha-linolenic acid, eicosapentaenoic acid, docosapentaenoic acid, docosahexaenoic acid), and the corresponding data for AD from a genome-wide association meta-analysis of 63,926 individuals (21,982 diagnosed AD cases, 41,944 controls). Results None of the genetically predicted PUFAs was significantly associated with AD risk; odds ratios (95% confidence interval) per 1 SD increase in PUFA levels were 0.98 (0.93, 1.03) for linoleic acid, 1.01 (0.98, 1.05) for arachidonic acid, 0.96 (0.88, 1.06) for alpha-linolenic acid, 1.03 (0.93, 1.13) for eicosapentaenoic acid, 1.03 (0.97, 1.09) for docosapentaenoic acid, and 1.01 (0.81, 1.25) for docosahexaenoic acid. Conclusions This study did not support the hypothesis that PUFAs decrease AD risk.

Effect of carrot supplementation on nutritional value of insects: a case study with Jamaican field cricket (Gryllus assimilis)

2021 ◽  
pp. 1-10
Author(s):  
M. Kulma ◽  
D. Petříčková ◽  
M. Kurečka ◽  
Z. Kotíková ◽  
J. Táborský ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fatty Acid Profile ◽  
Nutritional Value ◽  
Field Cricket ◽  
Feed Conversion ◽  
Beneficial Effects ◽  
Total Fatty Acids ◽  
Β Carotene

The fortification of feed is known to be a useful tool to manipulate the nutritional value of insects. This study aimed to reveal the effect of carrot supplement duration on the basic nutrients, fatty acid profile, carotenes, and tocopherols. Jamaican crickets (Gryllus assimilis) were provided with chicken feed supplemented with carrots ad libitum for 0, 3, 7, 14 and 60 days prior to harvest. The supplementation increased the levels of α-carotene and β-carotene in the insects. The content of carotenoids did not significantly differ between groups fed carrot for 14 (α-carotene = 13.7 μg/g DM; β-carotene = 34.6 μg/g DM) and 60 days (α-carotene = 15.2 μg/g DM; β-carotene = 37.2 μg/g DM), suggesting that the capacity of the insects to store carotenes is limited. Some carotenoids (α-carotene = 5.4 μg/g DM; β-carotene = 12.8 μg/g DM) were found in crickets given carrot supplementation for only 3 days prior to harvest. Carrot supplementation affected the fatty acid profile of the insects. Crickets fed carrot for 60 and 14 days contained lower levels of SFA (32.00 and 32.74% of total fatty acids respectively) and higher levels of PUFA (33.06 and 32.49% of total fatty acids respectively) in comparison with insects fed for 0, 3 or 7 days. No influence of carrot supplementation was observed in feed conversion, tocopherols and basic nutrients (proteins, lipids, ash). Conclusively, the paper showed some beneficial effects of carrot supplementation, when the highest levels of carotenoids were reached by 14 and 60 days of carrot provision. Therefore, the whole life supplementation seems to be not necessary to reach the increased content of carotenoids in crickets.

scholarly journals Maternal docosahexaenoic acid supplementation and fetal accretion

2003 ◽  
Vol 90 (1) ◽  
pp. 135-145 ◽  
Author(s):  
Colette Montgomery ◽  
Brian K. Speake ◽  
Alan Cameron ◽  
Naveed Sattar ◽  
Lawrence T. Weaver
Keyword(s):  
Fatty Acids ◽  
Nervous System ◽  
Docosahexaenoic Acid ◽  
Venous Blood ◽  
Double Blind Study ◽  
Double Blind ◽  
Blood Samples ◽  
Total Fatty Acids ◽  
Maternal Supplementation ◽  
Maternal Status

Docosahexaenoic acid (DHA) (22:6n−3) is a polyunsaturated fatty acid that is an essential constituent of membranes, particularly of the nervous system. Infants acquire DHA from their mothers, either prenatally via the placenta or postnatally in milk. The present study aimed to test the hypothesis that maternal supplementation during the second and third trimesters of pregnancy enriches maternal and/or fetal DHA status. In a randomised, prospective, double-blind study 100 mothers received either fish-oil capsules containing 400mg DHA/g (200mg/d) (n 50), or placebo containing 810mg oleic acid/g (400mg/d) (n 50) from 15 weeks gestation until term. Venous blood samples were obtained from mothers at 15, 28 and 40 weeks, and from the umbilical cord at birth. Total fatty acids in plasma and erythrocytes were analysed by GC–MS. There were no significant differences between maternal groups in baseline DHA, as a proportion of total fatty acids (g/100g total fatty acids) or concentration (nmol/ml), in plasma and erythrocytes. DHA concentrations in plasma at 28 weeks (P=0·02) and erythrocytes at both 28 weeks (P=0·03) and term (P=0·02) were 20% higher in supplemented mothers than the placebo group. DHA accounted for a higher proportion of total fatty acids in erythrocytes of supplemented mothers at 28 weeks (P=0·003) and term (P=0·01). There were no significant differences between groups in DHA (g/100g total fatty acids or nmol/l) in cord blood. Maternal DHA status was maximal in mid-trimester and declined to term, at a lower rate in supplemented compared with unsupplemented mothers. Maternal DHA supplementation significantly increases maternal DHA status and limits the last trimester decline in maternal status, aiding preferential transfer of DHA from mother to fetus.

Oils rich in docosahexaenoic acid in livers of sharks from temperate Australian waters

1998 ◽  
Vol 49 (7) ◽  
pp. 763 ◽  
Author(s):  
Peter D. Nichols ◽  
Michael J. Bakes ◽  
Nicholas G. Elliott
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Docosahexaenoic Acid ◽  
Wax Ester ◽  
Shark Species ◽  
Flame Ionization ◽  
Total Fatty Acids ◽  
Wet Weight ◽  
Galeorhinus Galeus ◽  
Direct Use

The livers from the two main commercially-targeted shark species in southern Australia (Mustelus antarcticus, gummy shark; Galeorhinus galeus, school shark), together with Squalus acanthias (white-spotted spurdog), were analysed for oil content and composition, and fatty acid composition. The yield of oil from the liver was 30-64% (wet weight) for M. antarcticus and 50-53% (wet weight) for G. galeus. Lipid classes were determined by thin-layer chromatography with flame ionization detection, with the major lipid being triacylglycerol (≥95%); other minor lipids were polar lipid, wax ester, sterol (mainly cholesterol) and free fatty acid. Long-chain ω-3 polyunsaturated fatty acids accounted for between 33% and 39% of the total fatty acids in all three species, and docosahexaenoic acid (DHA) concentrations were between 13% and 18%. The liver oils of M. antarcticus and G. galeus and other shark species may be an attractive source of w-3 fatty acids, specifically DHA, for direct use and/or for adding further value.

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