scholarly journals Taking Advantage of Bacterial Adaptation in Order to Optimize Industrial Production of Dry Propionibacterium freudenreichii

2019 ◽  
Vol 7 (10) ◽  
pp. 477 ◽  
Author(s):  
Floriane Gaucher ◽  
Valérie Gagnaire ◽  
Houem Rabah ◽  
Marie-Bernadette Maillard ◽  
Sylvie Bonnassie ◽  
...  
Keyword(s):  
Spray Drying ◽  
Industrial Production ◽  
Compatible Solutes ◽  
Protective Mechanism ◽  
Scale Production ◽  
Propionibacterium Freudenreichii ◽  
Bacterial Adaptation ◽  
Heat Adaptation ◽  
Technological Stresses ◽  
And Storage

Propionibacterium freudenreichii is a beneficial bacterium, used both as a probiotic and as a cheese starter. Large-scale production of P. freudenreichii is required to meet growing consumers’ demand. Production, drying and storage must be optimized, in order to guarantee high P. freudenreichii viability within powders. Compared to freeze-drying, spray drying constitutes the most productive and efficient, yet the most stressful process, imposing severe oxidative and thermal constraints. The aim of our study was to provide the tools in order to optimize the industrial production of dry P. freudenreichii. Bacterial adaptation is a well-known protective mechanism and may be used to improve bacterial tolerance towards technological stresses. However, the choice of bacterial adaptation type must consider industrial constraints. In this study, we combined (i) modulation of the growth medium composition, (ii) heat-adaptation, and (iii) osmoadaptation, in order to increase P. freudenreichii tolerance towards technological stresses, including thermal and oxidative constraints, using an experimental design. We further investigated optimal growth and adaptation conditions, by monitoring intracellular compatible solutes accumulation. Glucose addition, coupled to heat-adaptation, triggered accumulation of trehalose and of glycine betaine, which further provided high tolerance towards spray drying and storage. This work opens new perspectives for high quality and fast production of live propionibacteria at the industrial scale.

scholarly journals Hyperconcentrated Sweet Whey, a New Culture Medium That Enhances Propionibacterium freudenreichii Stress Tolerance

2016 ◽  
Vol 82 (15) ◽  
pp. 4641-4651 ◽  
Author(s):  
Song Huang ◽  
Houem Rabah ◽  
Julien Jardin ◽  
Valérie Briard-Bion ◽  
Sandrine Parayre ◽  
...  
Keyword(s):  
Spray Drying ◽  
Energy Efficient ◽  
Culture Medium ◽  
Intestinal Inflammation ◽  
Compatible Solutes ◽  
Growth Media ◽  
Efficient Production ◽  
Propionibacterium Freudenreichii ◽  
Content Type ◽  
Sweet Whey

ABSTRACTPropionibacterium freudenreichiiis used as a cheese-ripening starter and as a probiotic. Its reported physiological effects at the gut level, including modulation of bifidobacteria, colon epithelial cell proliferation and apoptosis, and intestinal inflammation, rely on active metabolismin situ. Survival and activity are thus key factors determining its efficacy, creating stress adaptation and tolerance bottlenecks for probiotic applications. Growth media and growth conditions determine tolerance acquisition. We investigated the possibility of using sweet whey, a dairy by-product, to sustainP. freudenreichiigrowth. It was used at different concentrations (dry matter) as a culture medium. Using hyperconcentrated sweet whey led to enhanced multistress tolerance acquisition, overexpression of key stress proteins, and accumulation of intracellular storage molecules and compatible solutes, as well as enhanced survival upon spray drying. A simplified process from growth to spray drying of propionibacteria was developed using sweet whey as a 2-in-1 medium to both cultureP. freudenreichiiand protect it from heat and osmotic injury without harvesting and washing steps. As spray drying is far cheaper and more energy efficient than freeze-drying, this work opens new perspectives for the sustainable development of new starter and probiotic preparations with enhanced robustness.IMPORTANCEIn this study, we demonstrate that sweet whey, a dairy industry by-product, not only allows the growth of probiotic dairy propionibacteria, but also triggers a multitolerance response through osmoadaptation and general stress response. We also show that propionibacteria accumulate compatible solutes under these culture conditions, which might account for the limited loss of viability after spray drying. This work opens new perspectives for more energy-efficient production of dairy starters and probiotics.

Increased Production of Recombinant O-Phospho-L-Serine Sulfhydrylase from the Hyperthermophilic Archaeon Aeropyrum pernix K1 Using Escherichia coli

2019 ◽  
Vol 8 (1) ◽  
pp. 15-23
Author(s):  
Takashi Nakamura ◽  
Emi Takeda ◽  
Tomoko Kiryu ◽  
Kentaro Mori ◽  
Miyu Ohori ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Industrial Production ◽  
Codon Optimization ◽  
Scale Production ◽  
Enzymatic Production ◽  
Hyperthermophilic Archaeon ◽  
E Coli ◽  
Aeropyrum Pernix ◽  
Natural Amino Acids

Background: O-phospho-L-serine sulfhydrylase from the hyperthermophilic archaeon Aeropyrum pernix K1 (ApOPSS) is thermostable and tolerant to organic solvents. It can produce nonnatural amino acids in addition to L-cysteine. Objective: We aimed to obtain higher amounts of ApOPSS compared to those reported with previous methods for the convenience of research and for industrial production of L-cysteine and non-natural amino acids. Method: We performed codon optimization of cysO that encodes ApOPSS, for optimal expression in Escherichia coli. We then examined combinations of conditions such as the host strain, plasmid, culture medium, and isopropyl β-D-1-thiogalactopyranoside (IPTG) concentration to improve ApOPSS yield. Results and Discussion: E. coli strain Rosetta (DE3) harboring the expression plasmid pQE-80L with the codon-optimized cysO was cultured in Terrific broth with 0.01 mM IPTG at 37°C for 48 h to yield a 10-times higher amount of purified ApOPSS (650 mg·L-1) compared to that obtained by the conventional method (64 mg·L-1). We found that the optimal culture conditions along with codon optimization were essential for the increased ApOPSS production. The expressed ApOPSS had a 6-histidine tag at the N-terminal, which did not affect its activity. This method may facilitate the industrial production of cysteine and non-natural amino acids using ApOPSS. Conclusion: We conclude that these results could be used in applied research on enzymatic production of L-cysteine in E. coli, large scale production of non-natural amino acids, an enzymatic reaction in organic solvent, and environmental remediation by sulfur removal.

scholarly journals Growth and nitrogen removal characteristics of Halomonas sp. B01 under high salinity

2019 ◽  
Vol 69 (13) ◽  
pp. 1425-1433 ◽  
Author(s):  
Te Wang ◽  
Zhengzhong Jiang ◽  
Wenbo Dong ◽  
Xiaoya Liang ◽  
Linghua Zhang ◽  
...  
Keyword(s):  
Mrna Expression ◽  
High Salinity ◽  
Halophilic Bacteria ◽  
Compatible Solutes ◽  
Aerobic Denitrification ◽  
Protective Mechanism ◽  
Sod Activity ◽  
N Removal ◽  
Nacl Concentration ◽  
Expression Abundance

Abstract Purpose At present, the nitrogen (N) removal efficiency of the microbial treatment in the high-salinity nitrogenous wastewaters is relatively low. Study on the N removal behavior and properties of moderately halophilic bacteria Halomonas under high salinity is of great significance for the microbial treatment of high-salinity nitrogenous wastewater. Methods The response mechanism of Halomonas sp. B01 to high osmotic pressure stress was investigated by measuring the compatible solute ectoine concentration and superoxide dismutase (SOD) activity. The salt tolerance during growth and N removal of the strain was evaluated by measuring the activities of growth-related and N removal–related enzymes and the mRNA expression abundance of ammonia monooxygenase-encoding gene (amoA). The process of simultaneous heterotrophic nitrification and aerobic denitrification (SND) under high salinity was described by measuring the concentration of inorganic N. Result Halomonas sp. B01 synthesized ectoine under NaCl stress, and the intracellular ectoine concentration increased with increased NaCl concentration in the growth medium. When the NaCl concentration of the medium reached 120 g L−1, the malondialdehyde concentration and SOD activity were significantly increased to 576.1 μg mg−1 and 1.7 U mg−1, respectively. The growth-related and N removal–related enzymes of the strain were active or most active in medium with 30–60 g L−1 NaCl. The amoA of the strain cultured in medium with 60 g L−1 NaCl had the highest mRNA expression abundance. In the N removal medium containing 60 g L−1 NaCl and 2121 mg L−1 NH4+-N, SND by Halomonas sp. B01 was performed over 96 h and the N removal rate reached 98.8%. Conclusion In addition to the protective mechanism of synthetic compatible solutes, Halomonas sp. B01 had the repair mechanism of SOD for lipid peroxidation. The growth-related and N removal–related enzymes of the strain were most active at a certain salt concentration; amoA also had the highest mRNA expression abundance under high salinity. Halomonas sp. B01 could efficiently perform N removal by SND under high salinity.

scholarly journals Peculiarities of solving the problems of modern logistics in high-rise construction and industrial production

2018 ◽  
Vol 33 ◽  
pp. 03076
Author(s):  
Anatoliy E. Rubtsov ◽  
Elena V. Ushakova ◽  
Tamara V. Chirkova
Keyword(s):  
Structural Optimization ◽  
Industrial Production ◽  
Distribution Network ◽  
Organization Structure ◽  
Modern Logistics ◽  
Logistics System ◽  
Modes Of Operation ◽  
High Rise ◽  
Construction Organization ◽  
And Storage

Basing on the analysis of the enterprise (construction organization) structure and infrastructure of the entire logistics system in which this enterprise (construction organization) operates, this article proposes an approach to solve the problems of structural optimization and a set of calculation tasks, based on customer orders as well as on the required levels of insurance stocks, transit stocks and other types of stocks in the distribution network, modes of operation of the in-company transport and storage complex and a number of other factors.

scholarly journals From optimization of synbiotic microparticles prepared by spray-drying to development of new functional carrot juice

2014 ◽  
Vol 20 (4) ◽  
pp. 549-564 ◽  
Author(s):  
Tanja Petreska-Ivanovska ◽  
Lidija Petrushevska-Tozi ◽  
Anita Grozdanov ◽  
Rumenka Petkovska ◽  
Jasmina Hadjieva ◽  
...  
Keyword(s):  
Spray Drying ◽  
Cold Storage ◽  
Carrot Juice ◽  
Encapsulated Cells ◽  
Gastrointestinal Conditions ◽  
High Viability ◽  
Formulation Factors ◽  
Full Factorial ◽  
And Storage ◽  
Design Experiments

Lactobacillus casei loaded chitosan-Ca-alginate microparticles enriched with the prebiotic fructooligosaccharide were prepared using spray-drying method associated with the polymers complexation and cross-linking with calcium. The concentrations of the formulation factors of alginate, chitosan and CaCl2 were optimized using 23 full factorial design. Experiments showed that microparticles with favorable physicochemical properties and high probiotic viability during preparation and storage could be obtained when 40 mg/g sodium alginate, 5 mg/g chitosan and 50 mg/g CaCl2 is used. Stability of L. casei during microencapsulation was identified by FTIR spectroscopy. The viability of the probiotic in the optimal formulation of synbiotic microparticles remained above the therapeutic minimum during incubation of 24 hours in simulated gastrointestinal conditions (7.67?0.4 log cfu/g) as well as after 3 months of cold storage (8.1?0.6 log cfu/g). High viability of L. casei was maintained during 6 weeks of cold storage when carrot juice was enriched with encapsulated cells. The effective preservation of L. casei into synbiotic microparticles provided production of new non-dairy functional food as an alternative of the population who is at risk of lactose intolerance.

scholarly journals Spray drying as a strategy for biosurfactant recovery, concentration and storage

SpringerPlus ◽  
2014 ◽  
Vol 3 (1) ◽  
pp. 49 ◽  
Author(s):  
Gisely S Barcelos ◽  
Lívia C Dias ◽  
Péricles L Fernandes ◽  
Rita Fernandes ◽  
Arnaldo C Borges ◽  
...  
Keyword(s):  
Spray Drying ◽  
And Storage

Changes in Concentration of Aflatoxin M1 during Manufacture and Storage of Skim Milk Powder

2006 ◽  
Vol 69 (3) ◽  
pp. 682-685 ◽  
Author(s):  
ORGUN DEVECİ ◽  
EMEL SEZGİN
Keyword(s):  
Spray Drying ◽  
Milk Powder ◽  
Skim Milk ◽  
Skim Milk Powder ◽  
Aflatoxin M1 ◽  
Weight Changes ◽  
Sample Weight ◽  
Powder Samples ◽  
And Storage ◽  
Different Levels

In this study, skim milk powder was produced from cow's milk contaminated artificially with aflatoxin M1 (AFM1) at two different levels, 1.5 and 3.5 μg/liter (ppb), and the effects of process stages on the AFM1 contents were investigated. Pasteurization, concentration, and spray drying caused losses of about 16, 40, and 68%, respectively, in AFM1 content of the milk contaminated with 1.5 μg/liter AFM1, and losses of 12, 35, and 59%, respectively, in the milk contaminated with 3.5 μg/liter AFM1. These losses were found to be statisticially significant at the level of P < 0.01. After 3- and 6-month storage periods, AFM1 content of the skim milk powder produced from milk with 1.5 μg/liter AFM1 decreased by 2 and 5%, respectively, whereas these rates were 2 and 4%, respectively, for the skim milk powders made from milk with 3.5 μg/liter AFM1 (after adjustment for sample weight). Changes in AFM1 content of milk powder samples were found statistically insignificant (P > 0.05 and P > 0.01) for 3- and 6-month storage periods.

Spray Drying of Spinach Juice: Characterization, Chemical Composition, and Storage

2017 ◽  
Vol 82 (12) ◽  
pp. 2873-2884 ◽  
Author(s):  
Gülşah Çalışkan Koç ◽  
Safiye Nur Dirim
Keyword(s):  
Chemical Composition ◽  
Spray Drying ◽  
And Storage
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