scholarly journals Nitrogen Removal Performance and Metabolic Pathways Analysis of a Novel Aerobic Denitrifying Halotolerant Pseudomonas balearica Strain RAD-17

2020 ◽  
Vol 8 (1) ◽  
pp. 72 ◽  
Author(s):  
Yunjie Ruan ◽  
Mohammad J. Taherzadeh ◽  
Dedong Kong ◽  
Huifeng Lu ◽  
Heping Zhao ◽  
...  
Keyword(s):  
Nitrogen Removal ◽  
Removal Rate ◽  
Inorganic Nitrogen ◽  
Ammonium Nitrogen ◽  
Effluent Treatment ◽  
Aerobic Denitrification ◽  
Recirculating Aquaculture ◽  
Pathways Analysis ◽  
Total Ammonium ◽  
Pseudomonas Balearica

An aerobic denitrification strain, Pseudomonas balearica RAD-17, was identified and showed efficient inorganic nitrogen removal ability. The average NO3−-N, NO2−-N, and total ammonium nitrogen (TAN) removal rate (>95% removal efficiency) in a batch test was 6.22 mg/(L∙h), 6.30 mg/(L∙h), and 1.56 mg/(L∙h), respectively. Meanwhile, optimal incubate conditions were obtained through single factor experiments. For nitrogen removal pathways, the transcriptional results proved that respiratory nitrate reductases encoded by napA, which was primarily performed in aerobic denitrification and cell assimilation, were conducted by gluS and gluD genes for ammonium metabolism. In addition, adding the strain RAD-17 into actual wastewater showed obvious higher denitrification performance than in the no inoculum group (84.22% vs. 22.54%), and the maximum cell abundance achieved 28.5 ± 4.5% in a ratio of total cell numbers. Overall, the efficient nitrogen removal performance plus strong environmental fitness makes the strain RAD-17 a potential alternative for RAS (recirculating aquaculture system) effluent treatment.

scholarly journals Ammonium removal by a novel heterotrophic nitrifying and aerobic denitrifying bacterium Pseudomonas stutzeri KTB from wastewater

2015 ◽  
Vol 50 (3) ◽  
pp. 219-227 ◽  
Author(s):  
Maohong Zhou ◽  
Hairen Ye ◽  
Xiaowei Zhao
Keyword(s):  
Nitrogen Removal ◽  
Removal Rate ◽  
Pseudomonas Stutzeri ◽  
Culture Conditions ◽  
Aerobic Denitrification ◽  
Ammonium Concentration ◽  
Logarithmic Phase ◽  
Ammonium Removal ◽  
Removal Ratio ◽  
Initial Ph

The effects of culture conditions on a newly isolated Pseudomonas stutzeri KTB's ability to simultaneously perform heterotrophic nitrification and aerobic denitrification were investigated to determine its potential of application in nitrogen removal from wastewater. The results from experiments in the presence of 10 mmol/L of ammonium were as follows: succinate was the preferred carbon source, and the optimum C/N ratio, temperature, and initial pH were 10, 30 °C, and 7–8, respectively. Nitrogen removal took place not only in the logarithmic phase but also in the stationary phase. Under the optimum conditions, the nitrogen removal rate increased as the ammonium concentration elevated, until it was as high as 60 mmol/L. Meanwhile, the maximum specific growth rate decreased. The highest nitrogen removal rate of 0.977 mmol/L/h was observed at 60 mmol/L of ammonium and the maximum removal ratio of 85.6% at 40 mmol/L when the bacterial treatment for 48 h was completed. The strain was vulnerable to even higher ammonium loads. When incubated in anaerobically digested hennery wastewater containing 43.85 mmol/L of ammonium and 2.32 mmol/L of nitrate, the removal ratio and rate reached 82.4% and 0.397 mmol/L/h, respectively. The strain might be a great candidate for ammonium removal from wastewater.

scholarly journals In Situ Nutrient Removal from Rural Runoff by A New Type Aerobic/Anaerobic/Aerobic Water Spinach Wetlands

Water ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 1100 ◽  
Author(s):  
Ya-Wen Wang ◽  
Hua Li ◽  
You Wu ◽  
Yun Cai ◽  
Hai-Liang Song ◽  
...  
Keyword(s):  
Nitrogen Removal ◽  
Constructed Wetland ◽  
Removal Rate ◽  
Oxygen Demand ◽  
Ammonium Nitrogen ◽  
Water Spinach ◽  
Nitrogen And Phosphorus ◽  
The Matrix ◽  
New Type ◽  
Nitrification And Denitrification

Rural runoff with abundant nutrients has become a great threat to aquatic environment. Hence, more and more attention has been focused on nutrients removal. In this study, an improved aerobic/anaerobic/aerobic three-stage water spinach constructed wetland (O-A-O-CW) was used to improve the removal of nitrogen and phosphorus of rural runoff. The removal rate of the target pollutants in O-A-O-CW was compared with the common matrix flow wetland as well as the no-plant wetland. The results showed that the O-A-O-CW significantly increased the chemical oxygen demand, total phosphorus, ammonium-nitrogen, nitrate, and total nitrogen removal rate, and the corresponding removal rate was 55.85%, 81.70%, 76.64%, 89.78%, and 67.68%, respectively. Moreover, the best hydraulic condition of the wetland, including hydraulic retention time and hydraulic loading, was determined, which were 2 days and 0.45 m3·m−2·day−1, respectively. Furthermore, the removal mechanism of the constructed wetland was thoroughly studied, which included the adsorption of nitrogen and phosphorus by the matrix and water spinach, and the nitrification and denitrification by the bacteria. The results demonstrated that the mechanisms of nitrogen removal in the new type wetland were principally by the nitrification and denitrification process. Additionally, adsorption and precipitation by the matrix are mainly responsible for phosphorus removal. These results suggested that the new O-A-O-CW can efficiently removal nutrients and enhance the water quality of the rural runoff.

scholarly journals Identification and Characterization of Janthinobacterium svalbardensis F19, a Novel Low-C/N-Tolerant Denitrifying Bacterium

2019 ◽  
Vol 9 (9) ◽  
pp. 1937 ◽  
Author(s):  
Yinyan Chen ◽  
Peng Jin ◽  
Zhiwen Cui ◽  
Tao Xu ◽  
Ruojin Zhao ◽  
...  
Keyword(s):  
Nitrogen Removal ◽  
Removal Rate ◽  
Pcr Amplification ◽  
Domestic Wastewater ◽  
Heterotrophic Nitrification ◽  
Aerobic Denitrification ◽  
Nitrite Accumulation ◽  
Specific Pcr ◽  
Domestic Wastewater Treatment ◽  
Initial Ph

Herein, we isolated Janthinobacterium svalbardensis F19 from sludge sediment. Strain F19 can simultaneously execute heterotrophic nitrification and aerobic denitrification under aerobic conditions. The organism exhibited efficient nitrogen removal at a C/N ratio of 2:1, with an average removal rate of 0.88 mg/L/h, without nitrite accumulation. At a C/N ratio of 2, an initial pH of 10.0, a culturing temperature of 25 °C, and sodium acetate as the carbon source, the removal efficiencies of ammonium, nitrate, nitrite, and hydroxylamine were 96.44%, 92.32%, 97.46%, and 96.69%, respectively. The maximum removal rates for domestic wastewater treatment for ammonia and total nitrogen were 98.22% and 92.49%, respectively. Gene-specific PCR amplification further confirmed the presence of napA, hao, and nirS genes, which may contribute to the heterotrophic nitrification and aerobic denitrification capacity of strain F19. These results indicate that this bacterium has potential for efficient nitrogen removal at low C/N ratios from domestic wastewater.

An Experimental Study on Compatible Solute Ectoine Dosing of High Salinity Wastewater

2014 ◽  
Vol 955-959 ◽  
pp. 1907-1910
Author(s):  
Su Chen ◽  
Lei Chao ◽  
Ning Chen ◽  
Lin Shan Wang ◽  
Xue Shao ◽  
...  
Keyword(s):  
Nitrogen Removal ◽  
Waste Treatment ◽  
High Salinity ◽  
Removal Rate ◽  
Ammonia Nitrogen ◽  
Ammonium Nitrogen ◽  
Treatment Efficiency ◽  
Removal Rates ◽  
Cod Removal Rate ◽  
Salinity Wastewater

When the reactor is added with ectoine of concentrations of 0, 0.1, 1 and 10 mmol/L, the impacts on brine waste treatment efficiency are investigated. The results show that the outflow COD and ammonia nitrogen removal rates are the highest, when the ectoine concentration is 0.1 mmol/L. The brine waste treatment efficiency under addition of ectoine of 1 and 10 mmol/L is even worse than that without ectoine addition. It can be preliminarily determined that the best ectoine dosage is in between 0.1-1.0 mmol/L. When ectoine concentrations added in reactors are 0.2, 0.5, 0.8 and 1.0 mmol/L, the results show that the average reactor outflow COD and ammonia nitrogen removal rates are increased compared with those of reactor without adding ectoine. But when ectoine of 1.0 mmol/L is added, the outflow COD and ammonia nitrogen removal rates decrease. When ectoine dosage is 0.5 mmol/L, the reactor outflow COD and ammonia nitrogen values are the lowest, the removal rates are the highest, the average COD removal rate is 74.46%, and the average ammonium nitrogen removal rate is 54.97%. Compared with reactor without adding ectoine, COD and ammonium nitrogen removal rates are increased by 13.16% and 26.81%. Therefore, the best dosage of ectoine is 0.5 mmol/L.

scholarly journals Kinetic Modelling and Determination of Octyl Phenol Ethoxylate (OPE) and Bisphenol A (BPA) (used as plastic additives) Inhibition Constants for Nitrogen Conversion

2021 ◽  
Keyword(s):  
Kinetic Model ◽  
Bisphenol A ◽  
Nitrogen Removal ◽  
Removal Rate ◽  
Kinetic Modelling ◽  
Ammonium Nitrogen ◽  
Operating Conditions ◽  
Optimum Operating ◽  
Octyl Phenol ◽  
Inhibition Constants

<p>Conversion of ammonia to nitrate is sensitive to a number of inhibitors. There is limited information on the nitrification inhibition coefficient and kinetic model in the current literature. Octyl Phenol Ethoxylate (OPE) and Bisphenol A (BPA) inhibition constants were found in nitrogen removal using an activated sludge system. Firstly, OPE and BPA free wastewater was used to determine the optimum operating conditions. The effect of OPE and BPA concentration on system performance was investigated. The ammonium removal rate was less affected by lower OPE and BPA concentrations. When the BPA and OPE concentrations were increased from 0 mg/L to 30 mg/L, the outlet ammonium nitrogen concentrations were increased respectively from 2.8 mg/L to 49.8 mg/L and from 2.6 mg/L to 20.40 mg/L. Due to the inhibition created by these compounds on Nitrobacter, nitrite nitrogen increased in the medium. As the OPE and BPA concentrations increased, the conversion rate of the ammonium nitrogen into nitrate decreased. Based on the experimental results, a kinetic model was developed, and the OPE and BPA inhibition constants (KOPE and KBPA) were found to be 40.7 mg/L and 11.76 mg/L, respectively. In nitrogen removal, BPA created a higher inhibition effect in comparison to OPE.</p>

scholarly journals Newly established process combining partial hydrogenotrophic denitrification and anammox for nitrogen removal

2020 ◽  
Vol 82 (7) ◽  
pp. 1272-1284
Author(s):  
Kenta Shinoda ◽  
Rawintra Eamrat ◽  
Yuya Tsutsumi ◽  
Suphatchai Rujakom ◽  
Tippawan Singhopon ◽  
...  
Keyword(s):  
Nitrogen Removal ◽  
Production Efficiency ◽  
Inorganic Nitrogen ◽  
Amplicon Sequencing ◽  
Ammonium Nitrogen ◽  
Great Promise ◽  
Rrna Gene ◽  
Ammonium Oxidation ◽  
Initial Ph ◽  
Anammox Process

Abstract The anaerobic ammonium oxidation (anammox) process holds great promise for treating nitrogen-contaminated water; stable nitrite-nitrogen (-N) production is significant to anammox performance. In this study, partial hydrogenotrophic denitrification (PHD) was used to stably and efficiently produce -N from nitrate-nitrogen (-N). An investigation of the effects of initial pH on the PHD process revealed that a high -N production efficiency (77.9%) could be ensured by setting an initial pH of 10.5. A combined PHD-anammox process was run for more than three months with maximal ammonium-nitrogen (-N), -N, and total dissolved inorganic nitrogen removal efficiencies of 93.4, 98.0, and 86.9%, respectively. The -N to -N and -N to -N ratios indicated that various bioprocesses were involved in nitrogen removal during the anammox stage, and a 16S rRNA gene amplicon sequencing was performed to further clarify the composition of microbial communities and mechanisms involved in the nitrogen removal process.

scholarly journals The Nitrogen-Removal Efficiency of a Novel High-Efficiency Salt-Tolerant Aerobic Denitrifier, Halomonas Alkaliphile HRL-9, Isolated from a Seawater Biofilter

2019 ◽  
Vol 16 (22) ◽  
pp. 4451 ◽  
Author(s):  
Jilong Ren ◽  
Chenzheng Wei ◽  
Hongjing Ma ◽  
Mingyun Dai ◽  
Jize Fan ◽  
...  
Keyword(s):  
Removal Efficiency ◽  
Nitrogen Removal ◽  
Nitrogen Balance ◽  
High Efficiency ◽  
Aerobic Denitrification ◽  
Salt Tolerant ◽  
N Accumulation ◽  
Recirculating Aquaculture ◽  
N Removal ◽  
Recirculating Aquaculture Systems

Aerobic denitrification microbes have great potential to solve the problem of NO3−-N accumulation in industrialized recirculating aquaculture systems (RASs). A novel salt-tolerant aerobic denitrifier was isolated from a marine recirculating aquaculture system (RAS) and identified as Halomonas alkaliphile HRL-9. Its aerobic denitrification performance in different dissolved oxygen concentrations, temperatures, and C/N ratios was studied. Investigations into nitrogen balance and nitrate reductase genes (napA and narG) were also carried out. The results showed that the optimal conditions for nitrate removal were temperature of 30 °C, a shaking speed of 150 rpm, and a C/N ratio of 10. For nitrate nitrogen (NO3−-N) (initial concentration 101.8 mg·L−1), the sole nitrogen source of the growth of HRL-9, the maximum NO3−-N removal efficiency reached 98.0% after 24 h and the maximum total nitrogen removal efficiency was 77.3% after 48 h. Nitrogen balance analysis showed that 21.7% of NO3−-N was converted into intracellular nitrogen, 3.3% of NO3−-N was converted into other nitrification products (i.e., nitrous nitrogen, ammonium nitrogen, and organic nitrogen), and 74.5% of NO3−-N might be converted to gaseous products. The identification of functional genes confirmed the existence of the napA gene in strain HRL-9, but no narG gene was found. These results confirm that the aerobic denitrification strain, Halomonas alkaliphile HRL-9, which has excellent aerobic denitrification abilities, can also help us understand the microbiological mechanism and transformation pathway of aerobic denitrification in RASs.

Kinetic study on nitrification of ammonium nitrogen-enriched synthetic wastewater using activated sludge

2020 ◽  
Vol 81 (1) ◽  
pp. 62-70
Author(s):  
Roumi Bhattacharya ◽  
Debabrata Mazumder
Keyword(s):  
Activated Sludge ◽  
Nitrogen Removal ◽  
Suspended Solids ◽  
Removal Rate ◽  
Nitrogen Concentration ◽  
Ammonium Nitrogen ◽  
Synthetic Wastewater ◽  
Yield Coefficient ◽  
Ammonium Oxidation ◽  
Consecutive Reaction

Abstract Nitrification of ammonium nitrogen (NH4+-N)-bearing synthetic wastewater was performed in a batch-activated sludge reactor by varying the initial ammonium nitrogen concentration up to 400 mg/L at a pH of 8.1 ± 0.2 and temperature of 36 ± 2 °C for developing the process kinetics using acclimatised biomass. Maximum ammonium nitrogen removal efficiency of 98.3% was achieved with initial ammonium nitrogen and mixed liquor suspended solids concentration of 235 mg/L and 2,180 mg/L, respectively, at 48 h batch period. Based on the experimental results, kinetic constants for ammonia nitrogen removal following Monod's approach were obtained as maximum substrate removal rate coefficient = 0.057 per day, yield coefficient = 0.336 mg volatile suspended solids/mg ammonium nitrogen, half velocity constant = 12.95 mg NH4+-N/L and endogenous decay constant = 0.02 per day. Nitrification is a consecutive reaction with ammonium oxidation as the first step followed by nitrite oxidation. The overall rate of nitrite and nitrate formation was observed to be 1.44 per day and 0.34 per day, respectively.

Isolation and Characteristics of Heterotrophic Nitrification-Aerobic Denitrification Bacterium, Bacillus cereus X7 at High Salinity

2013 ◽  
Vol 864-867 ◽  
pp. 111-114 ◽  
Author(s):  
Ru Lei Yao ◽  
Li Na Qiu ◽  
Wei Wei Zhang ◽  
Ai Jun Gong ◽  
Zi Yu Wang ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Nitrogen Removal ◽  
High Salinity ◽  
Removal Rate ◽  
Aerobic Denitrification ◽  
16S Rdna Sequence ◽  
16S Rdna Sequence Analysis ◽  
N Removal ◽  
Nacl Concentration ◽  
Biological Nitrogen

Biological nitrogen removal has been focused on in wastewater treatment field recently. A strain X7 was isolated from the sediment of pickle foodstuff wastewater. Based on its 16S rDNA sequence analysis, X7 was identified as Bacillus cereus. At NaCl concentration of 20 g/L, NH4+-N removal rate achieved 99.18%, when NO2--N and NO3--N removal rates were 77.24% and 68.6%, respectively. When NaCl concentration ranged from 0 to 40 g/L, the removal rate of NH4+-N was more than 97.59%. Therefore, due to the high nitrogen removal rate and excellent salt tolerance, Bacillus cereus X7 had a broad application prospect in the biodenitrification of brine wastewater.

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