scholarly journals Impact of Fermentable Protein, by Feeding High Protein Diets, on Microbial Composition, Microbial Catabolic Activity, Gut Health and beyond in Pigs

2020 ◽  
Vol 8 (11) ◽  
pp. 1735
Author(s):  
Hanlu Zhang ◽  
Nikkie van der Wielen ◽  
Bart van der Hee ◽  
Junjun Wang ◽  
Wouter Hendriks ◽  
...  
Keyword(s):  
Microbial Community ◽  
Dietary Protein ◽  
Average Daily Gain ◽  
High Protein ◽  
Intestinal Morphology ◽  
Microbial Composition ◽  
Protein Levels ◽  
Catabolic Activity ◽  
High Protein Diets ◽  
Protein Diets

In pigs, high protein diets have been related to post-weaning diarrhoea, which may be due to the production of protein fermentation metabolites that were shown to have harmful effects on the intestinal epithelium in vitro. In this review, we discussed in vivo effects of protein fermentation on the microbial composition and their protein catabolic activity as well as gut and overall health. The reviewed studies applied different dietary protein levels, which was assumed to result in contrasting fermentable protein levels. A general shift to N-utilisation microbial community including potential pathogens was observed, although microbial richness and diversity were not altered in the majority of the studies. Increasing dietary protein levels resulted in higher protein catabolic activity as evidenced by increased concentration of several protein fermentation metabolites like biogenic amines in the digesta of pigs. Moreover, changes in intestinal morphology, permeability and pro-inflammatory cytokine concentrations were observed and diarrhoea incidence was increased. Nevertheless, higher body weight and average daily gain were observed upon increasing dietary protein level. In conclusion, increasing dietary protein resulted in higher proteolytic fermentation, altered microbial community and intestinal physiology. Supplementing diets with fermentable carbohydrates could be a promising strategy to counteract these effects and should be further investigated.

scholarly journals Fermented Feed Supplement Relieves Caecal Microbiota Dysbiosis and Kidney Injury Caused by High-Protein Diet in the Development of Gosling Gout

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2139
Author(s):  
Yumeng Xi ◽  
Yuanpi Huang ◽  
Ying Li ◽  
Junshu Yan ◽  
Zhendan Shi
Keyword(s):  
Dietary Protein ◽  
Kidney Injury ◽  
High Protein Diet ◽  
High Protein ◽  
Protein Diet ◽  
Feed Supplement ◽  
Protein Levels ◽  
Fermented Feed ◽  
High Protein Diets ◽  
Protein Diets

Firstly, forty-eight 1-day-old goslings were randomly allocated to four groups and were fed diets containing crude protein (CP) at different concentrations: 160, 180, 200, and 220 g/kg in Experiment One. We found a dose-dependent relationship between the dietary protein levels and morbidity of gosling gout. The concentration of serum uric acid (UA), creatinine (Cr), and urea nitrogen (UN), and the activity of xanthine oxidase in the 220CP groups were significantly higher than those in the low-protein diet groups. Beneficial microbes, including Akkermansia, Lactococcus, and Butyricicoccus were enriched in the ceca of healthy goslings, while the microbes Enterococcus, Enterobacteriaceae, and Bacteroides were enriched in those with gout. Then, we explored the effects of fermented feed on gosling gout caused by high-protein diets in Experiment Two. A total of 720 1-day-old goslings were randomly allotted to four experimental groups: CN (162.9 g/kg CP), CNF (167.5 g/kg CP, replacing 50 g/kg of the basal diet with fermented feed), HP (229.7 g/kg CP, a high-protein diet), and HPF (230.7 g/kg CP, replacing 50 g/kg of the high-protein diet with fermented feed). We found that the cumulative incidence of gout increased in the HP group compared with that in the control, but decreased in the HPF group compared to that in the HP group. Similarly, the concentration of serum UA in the HP group was higher than that in the CN group, but decreased in the HPF group. Meanwhile, compared with the HP group, using fermented feed in diets decreased the abundance of Enterococcus in the ceca of goslings, while increasing the abundance of Lactobacillus. These results suggest that appropriate dietary protein levels and the fermented feed supplement might relieve the kidney injury and gut microbiota dysbiosis caused by high-protein diets in the development of gosling gout.

Dietary protein paradox: decrease of amino acid availability induced by high-protein diets

1993 ◽  
Vol 264 (6) ◽  
pp. G1057-G1065 ◽  
Author(s):  
C. Moundras ◽  
C. Remesy ◽  
C. Demigne
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Dietary Protein ◽  
High Protein ◽  
Glutamine Metabolism ◽  
Metabolic Adaptation ◽  
Casein Diet ◽  
Serine Dehydratase ◽  
High Protein Diets ◽  
Protein Diets

The aim of the present study was to evaluate the effect of changes in dietary protein level on overall availability of amino acids for tissues. For this purpose, rats were adapted to diets containing various concentrations of casein (7.5, 15, 30, and 60%) and were sampled either during the postprandial or postabsorptive period. In rats fed the protein-deficient diet, glucogenic amino acids (except threonine) tended to accumulate in plasma, liver, and muscles. In rats fed high-protein diets, the hepatic balance of glucogenic amino acids was markedly enhanced and their liver concentrations were consistently depressed. This response was the result of a marked induction of amino acid catabolism (a 45-fold increase of liver threonine-serine dehydratase activity was observed with the 60% casein diet). The muscle concentrations of threonine, serine, and glycine underwent changes parallel to plasma and liver concentrations, and a significant reduction of glutamine was observed. During the postabsorptive period, adaptation to high-protein diets resulted in a sustained catabolism of most glucogenic amino acids, which accentuated the drop in their concentrations (especially threonine) in all the compartments studied. The time course of metabolic adaptation from a 60 to a 15% casein diet has also been investigated. Adaptation of alanine and glutamine metabolism was rapid, whereas that of threonine, serine, and glycine was delayed and required 7-11 days. This was paralleled by a relatively slow decay of liver threonine-serine dehydratase (T-SDH) activity in contrast to the rapid adaptation of pyruvate kinase activity after refeeding a high-carbohydrate diet.(ABSTRACT TRUNCATED AT 250 WORDS)

INFLUENCE OF PRIOR DIETARY PROTEIN LEVELS ON RESISTANCE TO THE STRESS OF PROTEIN DEPLETION

PEDIATRICS ◽  
1962 ◽  
Vol 29 (1) ◽  
pp. 90-96
Author(s):  
Oliver W. Vaughan ◽  
L. J. Filer ◽  
Helen Churella
Keyword(s):  
Plasma Protein ◽  
Protein Turnover ◽  
High Protein Diet ◽  
High Protein ◽  
Intravenous Injections ◽  
Protein Levels ◽  
Low Protein ◽  
Protein Group ◽  
High Protein Diets ◽  
Protein Diets

One-day-old piglets were fed diets of either 50% or 14% protein for 8 weeks; they were given intravenous injections with S35-methionine-labeled plasma protein and were given a nonprotein diet. The plasma protein turnover and the nitrogen excreted were estimated by measuring the S35 activity and the nitrogen in aliquots of blood, urine and feces. During a 102-day period of protein privation, the animals that had received the high-protein diet lost little weight, while pigs previously fed a low level of protein lost 4.4 kg. However the high-protein group had a considerably faster rate of plasma protein turnover, catabolized a much large quantity of protein, and excreted more S35 and nitrogen than did the low-protein group. It is concluded that high-protein diets may make pigs less well able to cope with the stress of sudden protein deprivation.

scholarly journals Dietary protein intake is an independent and dose‐dependent regulator of hepatic anabolism in rats fed high protein diets

2008 ◽  
Vol 22 (S1) ◽  
Author(s):  
Laure Chevalier ◽  
Cécile Bos ◽  
Dalila Azzout‐Marniche ◽  
Gilles Fromentin ◽  
Daniel Tomé ◽  
...  
Keyword(s):  
Dietary Protein ◽  
Protein Intake ◽  
High Protein ◽  
Dietary Protein Intake ◽  
High Protein Diets ◽  
Protein Diets ◽  
Dose Dependent

scholarly journals The Effects of High-Protein Diets on Kidney Health and Longevity

2020 ◽  
Vol 31 (8) ◽  
pp. 1667-1679 ◽  
Author(s):  
Gang-Jee Ko ◽  
Connie M. Rhee ◽  
Kamyar Kalantar-Zadeh ◽  
Shivam Joshi
Keyword(s):  
Dietary Protein ◽  
Protein Intake ◽  
Observational Studies ◽  
Animal Protein ◽  
High Protein ◽  
High Protein Intake ◽  
High Protein Diets ◽  
Protein Diets ◽  
Kidney Health

Although high-protein diets continue to be popular for weight loss and type 2 diabetes, evidence suggests that worsening renal function may occur in individuals with—and perhaps without—impaired kidney function. High dietary protein intake can cause intraglomerular hypertension, which may result in kidney hyperfiltration, glomerular injury, and proteinuria. It is possible that long-term high protein intake may lead to de novo CKD. The quality of dietary protein may also play a role in kidney health. Compared with protein from plant sources, animal protein has been associated with an increased risk of ESKD in several observational studies, including the Singapore Chinese Health Study. Potential mediators of kidney damage from animal protein include dietary acid load, phosphate content, gut microbiome dysbiosis, and resultant inflammation. In light of such findings, adopting current dietary approaches that include a high proportion of protein for weight reduction or glycemic control should be considered with care in those at high risk for kidney disease. Given the possibility of residual confounding within some observational studies and the conflicting evidence from previous trials, long-term studies including those with large sample sizes are warranted to better ascertain the effects of high protein intake on kidney health.

scholarly journals POSTPARTUM CONCENTRATIONS OF ESTRADIOL AND PROGESTERONE IN THREE GENOTYPE OF SHEEP FED VARYING LEVELS OF PROTEIN

2021 ◽  
Vol 5 (1) ◽  
pp. 495-501
Author(s):  
A. M. Hassan ◽  
M. Nasir ◽  
A. Y. Abdullahi ◽  
M. H. Zango
Keyword(s):  
Protein Concentration ◽  
High Protein ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Ovarian Activity ◽  
Protein Levels ◽  
Low Protein ◽  
Ovarian Cyclicity ◽  
High Protein Diets ◽  
Protein Diets

The study aims to evaluate the effects of feeding different protein levels on the resumption of postpartum ovarian activity by measuring serum estradiol (E2) and progesterone (P4) concentrations bi-weekly starting one week postpartum in three genotypes of sheep (Yankasa, Uda and Balami). Eighteen (18) multiparous sheep with aged range of 2.5 – 3 years old consisting of six (6) each of Yankasa, Uda and Balami breeds were divided into three groups and reared on different protein concentrations. The protein concentrations were 15.2% (high), 9.5% (medium) and 6.5% (low). Results showed a gradual increase of estradiol in both Yankasa and Uda breeds fed low protein diet from 100 – 3000 concentration. However, Uda breed had the highest value of estradiol when both medium and high protein diets were fed to the three genotypes of sheep. Nevertheless, Yankasa sheep had more consistent values of progesterone when fed low protein diet compared to Balami and Uda. Also Uda breed recorded the highest progesterone values in both medium and high protein diets among the three genotypes followed by Yankasa sheep. All the three genotypes returned to estrous earlier on high protein concentration followed by those on medium protein level. It is concluded that increase protein concentration hastened resumption of ovarian cyclicity and thereby leading to increase fertility in sheep

Dietary protein-induced renal growth: correlation between renal IGF-I synthesis and hyperplasia

1994 ◽  
Vol 266 (4) ◽  
pp. C1037-C1045 ◽  
Author(s):  
E. Chin ◽  
C. A. Bondy
Keyword(s):  
Dna Synthesis ◽  
Dietary Protein ◽  
Growth Parameters ◽  
High Protein ◽  
Thick Ascending Limb ◽  
Mrna Levels ◽  
Renal Growth ◽  
Igf I ◽  
High Protein Diets ◽  
Protein Diets

Insulin-like growth factor I (IGF-I) and IGF binding protein 1 (IGFBP-1) mRNAs are colocalized in the medullary thick ascending limb (MTAL) of the rat nephron, a segment that undergoes selective growth in response to elevated dietary protein. In the present study, rats were fed isocaloric diets containing variable protein content (6-40%) for 1-7 days, and changes in fractional renal weight, MTAL length, and regional DNA synthesis were assayed and compared with local changes in IGF-I/IGFBP-1 mRNAs, as determined by quantitative in situ hybridization. Rats switched to high-protein diets demonstrated increased IGF-I and decreased IGFBP-1 mRNA levels in MTALs, whereas those switched to low protein showed inverse changes. The increase in renal IGF-I mRNA was maximal at 2 days and was closely paralleled by significant increases in fractional renal weight, DNA synthesis, and MTAL length. Similar changes were seen in vasopressin-deficient Brattleboro and growth hormone (GH)-deficient dwarf rats in response to high-protein diets, suggesting that the effects of dietary protein in this model are not mediated by vasopressin or GH. The close spatial and temporal correlation between changes in renal IGF-I expression and changes in regional growth parameters strongly supports a role for locally produced IGF-I in the induction of protein-induced renal growth.

Dairy proteins and the regulation of satiety and obesity

2007 ◽  
Vol 47 (9) ◽  
pp. 1051 ◽  
Author(s):  
Frank R. Dunshea ◽  
Ewa Ostrowska ◽  
Josie M. Ferrari ◽  
Harsharn S. Gill
Keyword(s):  
Insulin Sensitivity ◽  
Food Intake ◽  
Feed Intake ◽  
Dietary Protein ◽  
Protein Isolate ◽  
High Protein ◽  
Calcium Balance ◽  
Dairy Proteins ◽  
High Protein Diets ◽  
Protein Diets

Over the past decade there has been growing scientific evidence and public acceptance of the role that dietary protein plays in regulation of satiety, feed intake and obesity-related disorders. Dietary protein appears to suppress food intake and delay the return of hunger more than fats or carbohydrates in a manner not due to energy content alone. Also, high-protein diets support the maintenance of muscle mass when subjects reduce their energy intake, ensuring primarily adipose tissue loss. Some protein sources, particularly dairy, contain specific peptides or proteins that may elicit direct effects on satiety. The major proteins present in milk include β-lactalbumin, α-lactoglobulin, immunoglobulins, bovine serum albumin, and the various caseins. In addition, processed whey contains glycomacropeptide, which stimulates pancreatic and gastrointestinal secretion of hormones involved in satiety to a greater extent than whey alone. In the context of the literature, we show that a glycomacropeptide-rich whey protein isolate decreases feed intake and weight gain to a greater extent than a soy protein isolate in obese pigs. Also, insulin sensitivity is improved in pigs consuming high-protein diets, with these effects being independent of protein source. While, high-protein diets may decrease calcium balance and bone strength, it appears that these effects are attenuated by dairy proteins and dairy sources of calcium. These findings suggest that high-protein diets, and in particular those that contain whey proteins, may reduce hunger and food intake, thereby reducing fat deposition and improving insulin sensitivity.

303. NUTRIENT INTAKE OF BOS INDICUS HEIFERS DURING EARLY AND MID-GESTATION CAUSES CHANGES TO PLASMA CONCENTRATIONS OF TRIIODOTHYRONINE (T3) AND THYROXINE (T4) OF THEIR PROGENY

2010 ◽  
Vol 22 (9) ◽  
pp. 103
Author(s):  
G. C. Micke ◽  
T. M. Sullivan ◽  
V. E. A. Perry
Keyword(s):  
Birth Weight ◽  
Protein Intake ◽  
Nutrient Intake ◽  
Average Daily Gain ◽  
Plasma Concentrations ◽  
Postnatal Growth ◽  
High Protein ◽  
Economic Implications ◽  
High Protein Diets ◽  
Protein Diets

Fetal and postnatal growth are mediated by thyroid hormones (TH). Maternal nutrient intake during gestation can program postnatal TH concentrations. This may have significant economic implications for beef cattle production. We investigated the effect of feeding beef heifers high (H = 240%) and low (L = 70%) levels of recommended daily crude protein intake during the first and second trimesters of gestation in a two-by-two factorial design on progeny (n = 68) plasma concentrations of free and total triiodothyronine (FT3 and TT3) and free and total thyroxine (FT4 and TT4) from birth until weaning at 6 mo of age. Exposure to low compared to high protein diets during the second trimester resulted in increased plasma FT3 concentrations relative to TT3 (P = 0.04) at birth. For male progeny, exposure to low compared to high protein diets during the first trimester resulted in greater plasma FT4 concentrations from birth until weaning (P = 0.02). Also for males from birth until weaning, LH had greater plasma TT3 concentrations than HH (P < 0.01). For female progeny, HH had greater plasma TT3 concentrations relative to TT4 than HL from birth until weaning (P = 0.03). Plasma concentrations of FT3 were positively associated with average daily gain relative to birth weight at 1 (r = 0.41; P < 0.01) and 3 mo FT3 (r = 0.41; P < 0.01). Heifer protein intake during the first and second trimesters of gestation has a permanent effect to progeny plasma TH concentrations and these changes are associated with the postnatal growth pathway. (1) Micke GC, Sullivan TM, Magalhaes RJS, Rolls PJ, Norman ST, Perry VEA. Heifer nutrition during early- and mid-pregnancy alters fetal growth trajectory and birth weight. Anim Reprod Sci. 2010; 117: 1–10.

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