scholarly journals Effect of Colonization of Trichoderma harzianum on Growth Development and CBD Content of Hemp (Cannabis sativa L.)

2021 ◽  
Vol 9 (3) ◽  
pp. 518
Author(s):  
Ioanna Kakabouki ◽  
Alexandros Tataridas ◽  
Antonios Mavroeidis ◽  
Angeliki Kousta ◽  
Stella Karydogianni ◽  
...  
Keyword(s):  
Trichoderma Harzianum ◽  
Mycorrhizal Fungi ◽  
Cannabis Sativa ◽  
Arbuscular Mycorrhizal ◽  
Dry Weight ◽  
High Dose ◽  
Biotic And Abiotic Stresses ◽  
Significance Level ◽  
Randomized Design ◽  
Completely Randomized Design

Trichoderma harzianum, as a natural endophytic biocontrol agent, can ameliorate plant development, nutrient uptake, and resistance to biotic and abiotic stresses. This study aimed to investigate the effect of Trichoderma harzianum inoculation on agronomical and quality characteristics of two monoecious hemp (Cannabis sativa L.) varieties, Fedora 17 and Felina. A greenhouse pot experiment was conducted in a completely randomized design of two treatments of Trichoderma harzianum with a low and high dose of the fungus (T1 and T2). The significance of differences between treatments was estimated by using a Fisher’s test with a significance level p = 0.05. The root density of both varieties was significantly affected by treatments, and higher values were recorded in Fedora 17 (2.32 mm cm−3). The Arbuscular Mycorrhizal Fungi (AMF) colonization of the root system and the soil emission of CO2 were higher after the inoculation of Trichoderma harzianum. The highest values of plant height and dry weight were noticed for T2, especially in variety Felina. Trichoderma harzianum positively influenced characteristics of inflorescences such as their number, fresh weight moisture, and compactness in both varieties, while the dry weight, length, and dry yield of inflorescences were not improved. Finally, the fertigation of Trichoderma harzianum in hemp plants was beneficial by increasing the cannabidiol (CBD) content, especially in T2 treatment (4 × 1012 CFU kg−1).

scholarly journals Pengaruh Jenis Pupuk dan Tingkat Arbuskular Mikoriza terhadap Pertumbuhan dan Hasil Jahe (Zingiber officinale)

2014 ◽  
Vol 16 (2) ◽  
pp. 44
Author(s):  
Dhimas Taufika Putra ◽  
Samanhudi Samanhudi ◽  
Purwanto Purwanto
Keyword(s):  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Dry Weight ◽  
Zingiber Officinale ◽  
Chicken Manure ◽  
Growth And Yield ◽  
Fresh Weight ◽  
Randomized Design ◽  
Medical Plant ◽  
Completely Randomized Design

<p>Ginger (<em>Zingiber officinale</em>) is one kind of plants to be processed as traditional medicine, drinks, and spices. the smallest type of ginger that has many benefits as medical plant. Demand for ginger plants growing in the community is increasing. Additions of manure and arbuscular mychorrhizal fungi (AMF) can increase the quality and quantity of ginger. This was conducted to obtain the kinds of manure and levels of AMF to ginger to produce optimum growth and yield. This research used a completely randomized design (CRD) consisting of two treatment factors, namely planting medium and arbuscular mycorrhizal fungi (AMF). Results were analyzed by analysis of variance and if there is a real difference continued DMRT 5% level. The results showed that the differences in the kinds of manure and the levels of AMF increase growth and yield of ginger. Chicken manure application showed the highest results in the fresh weight of stover plant, dry weight of plant stover, fresh weight of plant rhizomes, and dry weight of the rhizomes. Whereas the additions of AMF 15 grams per plant in zeolite showed the highest results in the number of plant tillers, dry weight of plant stover, fresh weight of plant rhizomes, and dry weight of the rhizomes.</p>

scholarly journals Pemanfaatan Fungi Mikoriza Arbuskula dan Pot Organik untuk Meningkatkan Pertumbuhan Suren (Toona sinensis Roem.) di Persemaian Permanen IPB Dramaga

2020 ◽  
Vol 11 (2) ◽  
pp. 102-108
Author(s):  
Sri Wilarso Budi ◽  
Dwi Puspasari Arum Ramadhani
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Rice Husk ◽  
Mycorrhizal Fungi ◽  
Environmentally Friendly ◽  
Arbuscular Mycorrhizal ◽  
Dry Weight ◽  
Toona Sinensis ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Total Dry Weight

The cultivation of seedling which uses polybag caused the increase of polybag demand. Due to the rising demand of polybag, the rate of pollution caused by polybag waste is increasing as well, so that it requires an alternative to solve the problem by using organic based pot which is environmentally friendly and easily degraded. This study aimed to analyze the effect of Arbuscular Mycorrhizal Fungi (AMF) adding and the utilization of organic based pot to the growth of Suren (T. sinensis) seedling. This study was conducted using completely randomized design with two factor method, as well as observation for 16 weeks. The results showed that the interaction of organic pot composition treatment and AMF addition were significantly affected to the both parameters of height and diameter, while the treatment of organic pot composition was significantly affected to the parameter of total dry weight, moreover the treatment of AMF addition was significantly affected to the parameter of colonization of AMF. The results of this study indicated that the treatment of newspaper: rice husk gave better response of growth than other treatments. Keywords: Arbuscular Mycorrhizal Fungi, suren (T. sinensis), organic pot

scholarly journals RESPON PERTUMBUHAN Tithonia diversifolia TERHADAP INOKULASI CENDAWAN MIKORIZA ARBUSKULA (CMA)

Jurnal Solum ◽  
2011 ◽  
Vol 8 (2) ◽  
pp. 70
Author(s):  
Agustian Agustian ◽  
Mutia Faiza ◽  
Lusi Maira
Keyword(s):  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Dry Weight ◽  
Tithonia Diversifolia ◽  
Fresh Weight ◽  
Beneficial Effects ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Level Of Significance ◽  
Leaf Dry Weight

The success of symbiotic arbuscular mycorrhizal fungi (AMF) in plants was determined by three main factors namely by edaphic factors (environment), genetic and physiological plants and fungi. In this study AMF inoculations were attempted in Tithonia (Tithonia diversifolia) which is known as Mexican sunflower that is often used as a source of green manure. The purpose of this study was to find out AMF species that has the highest physiological compatibility with Tithonia and to study the effects of AMF species on Tithonia growth.  Experimental design used in this study was Completely Randomized Design (CRD) with 5 treatments and 3 replications. The data were statistically analyzed the variance, if the value of F-calculated was larger than that of F-table, then the analyses were continued using DNMRT at 5% level of significance. The treatment given were: without AMF, giving inoculant Glomus manihottis, Glomus etunicatum, Gigaspora margarita and a mixture of AMF. The number of spores that are given at each treatment was 100 spores / pot.  From this study it could be concluded that Tithonia species could be infected by the inoculated AMF. AMF infection had not yet demonstrated beneficial effects for Tithonia although the frequency of infection and the number of spores on rhizosphere were also high. Inoculation of G., Glomus manihottis, G. etunicatum, and a mixture of AMF did not have a significant influence on plant height, stem and leaf dry weight, fresh and dry weight of roots. Response of Tithonia growth were shown only on the fresh weight of stems and leaves that are found with the inoculation of G. margarita.Keywords: arbuscular mycorrhiza, growth, innoculation, Tithonia diversifolia,

scholarly journals Enhancement of clover growth by inoculation of P-solubilizing fungi and arbuscular mycorrhizal fungi

2010 ◽  
Vol 82 (3) ◽  
pp. 771-777 ◽  
Author(s):  
Edson L. Souchie ◽  
Rosario Azcón ◽  
Jose M. Barea ◽  
Eliane M.R. Silva ◽  
Orivaldo J. Saggin-Júnior
Keyword(s):  
Growth Rate ◽  
Aspergillus Niger ◽  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Trifolium Pratense ◽  
Arbuscular Mycorrhizal ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Environment Chamber ◽  
And Control

This study evaluated the synergism between several P-solubilizing fungi isolates and arbuscular mycorrhizal fungi to improve clover ( Trifolium pratense) growth in the presence of Araxá apatite. Clover was sown directly in plastic pots with 300g of sterilized washed sand, vermiculite and sepiolite 1:1:1 (v:v:v) as substrate, and grown in a controlled environment chamber. The substrate was fertilized with 3 g L-1 of Araxá apatite. A completely randomized design, in 8×2 factorial scheme (eight P-solubilizing fungi treatments with or without arbuscular mycorrhizal fungi)and four replicates were used. The P-solubilizing fungi treatments consisted of five Brazilian P-solubilizing fungi isolates (PSF 7, 9, 20, 21 and 22), two Spanish isolates ( Aspergillus niger and the yeast Yarowia lipolytica) and control (non-inoculated treatment). The greatest clover growth rate was recorded when Aspergillus niger and PSF 21 were co-inoculated with arbuscular mycorrhizal fungi. Aspergillus niger, PSF 7 and PSF 21 were the most effective isolates on increasing clover growth in the presence of arbuscular mycorrhizal fungi. Greater mycorrhizal colonization resulted in greater clover growth rate in most PSF treatments. PSF 7 was the best isolate to improve the establishment of mycorrhizal and rhizobia symbiosis.

scholarly journals Effectivity of Trichoderma harzianum as Bio-fungicide Against Moler Disease and Bio-stimulator of Shallot Growth

2020 ◽  
Vol 1 (1) ◽  
pp. 12
Author(s):  
Icha Rusita ◽  
Hadi Sasongko
Keyword(s):  
Trichoderma Harzianum ◽  
Dry Weight ◽  
Positive Control ◽  
Negative Control ◽  
Correlation Pattern ◽  
Randomized Design ◽  
Significant Difference ◽  
Wet Weight ◽  
Number Of Leaves ◽  
Completely Randomized Design

Moler disease is the primary disease that disturbs the shallot’ cultivation. Hence, it is beneficial to have preventive measures to mitigate the risk and to improve growth. The research objective is to know the effectivity of Trichoderma harzianum suspension as a bio-fungicide and bio-stimulator. The study used seven treatments, i.e., immersion of shallot seeds on negative control (distilled water), positive control (synthetic fungicide+ZPT 1g/liter), T.harzianum suspension with different concentrations of 2 ppm, 4 ppm, 6 ppm, 8 ppm, and 10 ppm, assigned in a Completely Randomized Design (CRD). Treatment that results in a significant level will be continued to the Least Significance Different (LSD) 5% test. The observed variables are the intensity of moler disease and the growth of shallot seeds. Experiment results show the significant difference between treatments. The optimal treatment to reduce the percentage of moler disease intensity is the immersion of shallot seeds in 10 ppm-concentrated T.harzianum suspension, while to improve the shallot growth is in 8 ppm-concentrated T.harzianum suspension. The intensity of moler disease has a negative correlation to the plant’s height and leaf’s diameter but shows no correlation pattern to the number of leaves, wet weight, dry weight, and length of roots.

scholarly journals Arbuscular mycorrhizal fungi in soil aggregates from fields of "murundus" converted to agriculture

2015 ◽  
Vol 50 (4) ◽  
pp. 313-321 ◽  
Author(s):  
Marco Aurélio Carbone Carneiro ◽  
Dorotéia Alves Ferreira ◽  
Edicarlos Damacena de Souza ◽  
Helder Barbosa Paulino ◽  
Orivaldo José Saggin Junior ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Soil Aggregates ◽  
Geometric Mean ◽  
Arbuscular Mycorrhizal ◽  
Spore Density ◽  
Extraradical Mycelium ◽  
Randomized Design ◽  
Conversion Time ◽  
Completely Randomized Design

The objective of this work was to evaluate the spore density and diversity of arbuscular mycorrhizal fungi (AMF) in soil aggregates from fields of "murundus" (large mounds of soil) in areas converted and not converted to agriculture. The experiment was conducted in a completely randomized design with five replicates, in a 5x3 factorial arrangement: five areas and three aggregate classes (macro-, meso-, and microaggregates). The evaluated variables were: spore density and diversity of AMF, total glomalin, total organic carbon (TOC), total extraradical mycelium (TEM), and geometric mean diameter (GMD) of soil aggregates. A total of 21 AMF species was identified. Spore density varied from 29 to 606 spores per 50 mL of soil and was higher in microaggregates and in the area with 6 years of conversion to agriculture. Total glomalin was higher between murundus in all studied aggregate classes. The area with 6 years showed lower concentration of TOC in macroaggregates (8.6 g kg-1) and in microaggregates (10.1 g kg-1). TEM was greater at the top of the murundus in all aggregate classes. GMD increased with the conversion time to agriculture. The density and diversity of arbuscular mycorrhizal spores change with the conversion of fields of murundus into agriculture.

scholarly journals ARBUSCULAR MYCORRHIZAL FUNGI AND DARK SEPTATE ENDOPHYTIC FUNGI ON THE BIOMASS DEVELOPMENT OF VETIVER GRASS

2018 ◽  
Vol 31 (3) ◽  
pp. 602-611 ◽  
Author(s):  
JESSICA SILVA SANTOS ◽  
JACILENE FRANCISCA SOUZA SANTOS ◽  
LÁZARA JOSSIKARLA DE OLIVEIRA LOPES ◽  
JOHNY DE JESUS MENDONÇA ◽  
FRANCISCO SANDRO RODRIGUES HOLANDA ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Endophytic Fungi ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Northeastern Brazil ◽  
Vetiver Grass ◽  
Grass Family ◽  
Randomized Design ◽  
Fast Development ◽  
Completely Randomized Design

ABSTRACT Vetiver grass is a member of the grass family Poaceae. Its fast development is probably due to the interaction with native microbiota, whose influence has not been studied yet. The objective of this work was to evaluate the colonization and development of the vetiver grass (Chrysopogon zizanioides (L.) Roberty) inoculated with arbuscular mycorrhizal fungi and dark septate endophytic fungi. The experimental design was a completely randomized design with six treatments (control, without mycorrhizal fungi, native inoculants, UFLA05 - Gigaspora albida, UFLA351 - Rhizoglomus clarum, UFLA372 - Claroideoglomus etunicatum, and UFLA401 - Acaulospora morrowiae), with three replicates each. Vetiver grass tillers as well as the native microbial inoculum were obtained from the Lower São Francisco river experimental area, located in Sergipe state, Northeastern Brazil. There was a negative interaction between all tested UFLAs mycorrhizal isolates and the native microbiota (mycorrhizal and endophytic fungi) in the treatments, especially when taking into consideration plant height and volume of roots. The effects of inoculation with UFLA isolates may have been influenced by the presence of the native mycorrhizal fungi and the dark septate endophytic fungi. Vetiver grass was responsive to the native inoculant. The mycorrhizal colonization of the vetiver grass was vesicular, but the formation of the arbuscules can be influenced by the interaction between the fungus, plant, and the environment.

scholarly journals Phosphate solubilization and synergism between P-solubilizing and arbuscular mycorrhizal fungi

2006 ◽  
Vol 41 (9) ◽  
pp. 1405-1411 ◽  
Author(s):  
Edson Luiz Souchie ◽  
Rosario Azcón ◽  
Jose Miguel Barea ◽  
Orivaldo José Saggin-Júnior ◽  
Eliane Maria Ribeiro da Silva
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Plant Nutrition ◽  
Phosphate Solubilization ◽  
Arbuscular Mycorrhizal ◽  
Aluminum Phosphate ◽  
Randomized Design ◽  
P Content ◽  
Completely Randomized Design ◽  
Presence And Absence

The objective of this work was to evaluate the ability of several P-solubilizing fungi to solubilize aluminum phosphate and Araxá apatite as well as the synergism between the P-solubilizing fungus, PSF 7, and arbuscular mycorrhizal fungi to promote clover growth amended with aluminum phosphate. Two experiments were carried out, the first under laboratory conditions and the second in a controlled environmental chamber. In the first experiment, PSF 7, PSF 9, PSF 21 and PSF 22 isolates plus control were incubated in liquid medium at 28ºC for eight days. On the 2nd, 4th and 8th day of incubation, pH and soluble P were determined. In the second experiment, clover was sowed in plastic pots containing 300 g of sterilized substrate amended with aluminum phosphate, 3 g L-1, in presence and absence of PSF 7 isolate and arbuscular mycorrhizal fungi. A completely randomized design, in factorial outline 2x2 (presence and absence of PSF 7 and arbuscular mycorrhizal fungi) and five replicates were used. In the first experiment, higher P content was detected in the medium containing aluminum phosphate. PSF 7 is the best fungi isolate which increases aluminum solubilization with major tolerance to Al3+. Clover growth was stimulated by presence of PSF 7 and arbuscular mycorrhizal fungi. There is synergism between microorganisms utilized to improve plant nutrition.

scholarly journals Revegetation of Critical Land with Gaharu (Aquilaria malaccensis) under Various Ameliorants Application

2021 ◽  
Vol 26 (1) ◽  
pp. 19
Author(s):  
Slamet Budi Yuwono ◽  
Alawiyah Alawiyah ◽  
Melya Riniarti ◽  
Dermiyati Dermiyati
Keyword(s):  
Mycorrhizal Fungi ◽  
Chemical Properties ◽  
Arbuscular Mycorrhizal ◽  
Dry Weight ◽  
P Uptake ◽  
Randomized Design ◽  
Aquilaria Malaccensis ◽  
Vesicular Arbuscular ◽  
Limestone Soil ◽  
Amf Inoculation

Rehabilitation of post-mining limestone soils is often a challenge due to a lack of nutrients and poor soil humus. The research aims to study the effect of various ameliorants on soil chemical properties, growth, and P uptake of gaharu plant (Aquilaria malaccensis) in post-mining limestone soil for revegetation of critical land. The research was conducted in a Completely Randomized Design with three replications. The treatments were P0 (Control, without ameliorant); P1 (Humic Acid, HA, 4 kg ha-1 ); P2 (Phosphate Rocks, PR, 350 kg ha-1 ); P3 (Vesicular Arbuscular Mycorrhizal Fungi, AMF, 500 spores plant-1 ); P4 (HA+AMF); P5 (PR+AMF); and P6 (HA+PR+AMF). Data were analyzed using ANOVA at 95% confidence level and continued with the LSD test. The use of various types of ameliorants (HA, RP, and AMF) significantly increased root length, root volume, wet and dry weight of roots, shoot wet and dry weight, and P uptake of gaharu tree. The best ameliorant in increasing gaharu tree growth was AMF (P3) treatment, and AMF combined with HA (P4) treatment. Thus, for revegetation of critical land, especially post-mining limestone land, using gaharu tree requires HA and AMF inoculation.

scholarly journals Growth Response of Four Conilon Coffee Varieties (Coffea canephora Pierre ex A. Froehner) to Different Shading Levels

2019 ◽  
Vol 11 (7) ◽  
pp. 29
Author(s):  
Bruno dos Passos Assis ◽  
Eduardo Gross ◽  
Norma Eliane Pereira ◽  
Marcelo Schramm Mielke ◽  
Gedeon Almeida Gomes Júnior
Keyword(s):  
Leaf Anatomy ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Coffea Canephora ◽  
Agroforestry Systems ◽  
Phytopathogenic Fungi ◽  
Randomized Design ◽  
Cow Urine ◽  
Collar Diameter ◽  
Completely Randomized Design

In order to select a conilon coffee (Coffea canephora Pierre ex A. Froehner) adapted to shade, four varieties (C153, JM2, LB1 and GG) were submitted to four shade levels (0, 30, 50 and 70) with evaluation of plant height, stem diameter, collar diameter, number of plagiotropic branches, number of fruits, root colonization by arbuscular mycorrhizal fungi and leaf anatomy. The experiment was carried out on a completely randomized design, in a 4 &times; 4 factorial scheme with ten replicates. We used organic compost based on cocoa shells for plant nutrition and cow urine for phytopathogenic fungi control. There was interaction between variety and shade factors for most of coffee characteristics analyzed. In general all coffee characteristics evaluated mainly fruit number and length of fruiting branches, significantly increased with increasing shade. Coffee varieties tested respond differently to the increasing of shade levels and leaf anatomy demonstrated the reduction of mesophyll thickness as the shading increased. The effect of shade levels on fruit yield fit to a positive linear regression for all four coffee varieties tested but the mycorrhizal colonization no presented differences among coffee varieties and shade levels. The varieties C153 and GG presented highlighted anatomical, growth and productive characteristics and can be indicated for shading cultivation, for example on agroforestry systems.

Sign in / Sign up

Export Citation Format

Share Document