scholarly journals Effect of Carbon Sources on Pyrite-Arsenopyrite Concentrate Bio-oxidation and Growth of Microbial Population in Stirred Tank Reactors

2021 ◽  
Vol 9 (11) ◽  
pp. 2350
Author(s):  
Aleksandr Bulaev ◽  
Aleksandra Nechaeva ◽  
Yuliya Elkina ◽  
Vitaliy Melamud
Keyword(s):  
Carbon Sources ◽  
Stirred Tank ◽  
Microbial Populations ◽  
Key Factors ◽  
Factors Affecting ◽  
Stirred Tank Reactors ◽  
Number Of Microorganisms ◽  
Different Temperatures ◽  
Sulfide Concentrates ◽  
Sulfide Concentrate

Tank bio-oxidation is a biohydrometallurgical technology widely used for metal recovery from sulfide concentrates. Since carbon availability is one of the key factors affecting microbial communities, it may also determine the rate of sulfide concentrate bio-oxidation. The goal of the present work was to evaluate the effect of carbon sources on the bio-oxidation of the concentrate containing 56% pyrite and 14% arsenopyrite at different temperatures (40 and 50 °C) in stirred tank reactors. CO2 was supplied into the pulp of the first reactor (about 0.01 L/min) and 0.02% (w/v) molasses was added to the pulp of the second one, and no additional carbon sources were used in the control tests. At 40 °C, 77% of pyrite and 98% of arsenopyrite were oxidized in the first reactor, in the second one, 73% of pyrite and 98% of arsenopyrite were oxidized, while in the control reactor, 27% pyrite and 93% arsenopyrite were oxidized. At 50 °C, in the first reactor, 94% of pyrite and 99% of arsenopyrite were oxidized, in the second one, 21% of pyrite and 94% of arsenopyrite were oxidized, while in the control reactor, 10% pyrite and 92% arsenopyrite were oxidized. The analysis of the microbial populations in the reactors revealed differences in the total number of microorganisms and their species composition. Thus, it was shown that the use of various carbon sources made it possible to increase the intensity of the concentrate bio-oxidation, since it affected microbial populations performing the process.

INFLUENCE OF TEMPERATURE AND CARBON SOURCE ON BIOXIDATION OF PYRITE-ARSENOPYRITE CONCENTRATE

2020 ◽  
pp. 361-363
Author(s):  
A. Nechaeva ◽  
Y. Elkina ◽  
V. Melamud
Keyword(s):  
Carbon Source ◽  
Microbial Communities ◽  
Carbon Sources ◽  
Microbial Populations ◽  
Effect Of Temperature ◽  
Influence Of Temperature ◽  
Carbon Nutrition ◽  
Sulfide Concentrate ◽  
Gold Bearing

The effect of temperature and sources of carbon nutrition on the composition of microbial communities carrying out the biooxidation of gold-bearing sulfide concentrate and on the efficiency of the biooxidation was investigated. It was shown that the use of additional carbon sources makes it possible to influence the activity of the biooxidation process and the composition of microbial populations.

scholarly journals Aerobic Denitrification Is Enhanced Using Biocathode of SMFC in Low-Organic Matter Wastewater

Water ◽  
2021 ◽  
Vol 13 (24) ◽  
pp. 3512
Author(s):  
Haochi Zhang ◽  
Dengfeng Hou ◽  
Shuai Zhang ◽  
Xian Cao ◽  
Hui Wang ◽  
...  
Keyword(s):  
Organic Matter ◽  
Microbial Fuel Cells ◽  
Carbon Sources ◽  
Aerobic Denitrification ◽  
Key Factors ◽  
Factors Affecting ◽  
Physicochemical Methods ◽  
Removal Technologies ◽  
Global Threat ◽  
Denitrification Process

Nitrate (NO3−) in wastewater is a rising global threat to ecological and health safety. A sufficient carbon source, as the electron donor, is essential in the conventional biological denitrification process. It is not appropriate to add extra carbon sources into specific water bodies in terms of material cost and secondary pollution. Thus, innovative NO3− removal technologies that are independent of carbon sources, are urgently needed. This study constructed sediment microbial fuel cells (SMFCs) for aerobic denitrification in low-organic matter wastewater and explored the key factors affecting denitrification efficiencies. The SMFC treatments removed 72–91% NO3− through two main denitrifying stages which were driven by carbon sources (COD) and generated electrons, respectively. After COD was fully consumed, denitrification efficiencies were enhanced in SMFC treatments by 24–47% using the generated electrons within 3 days. In this stage, the NO3− removal efficiencies were positively correlated with external current intensities (p < 0.05). The improved denitrification efficiencies were attributed to two enriched phyla in the SMFC cathode. The dominant genera also demonstrated the heterotrophic denitrifying capacity of the SMFC biocathode. Furthermore, electrical characteristics could be used to monitor or regulate the denitrification process in the SMFC system. In conclusion, this study presents an innovative treatment strategy that is economical and eco-friendly compared with conventional physicochemical methods.

Removal of lead by sulfate-reducing bacteria in anaerobic continuous stirred tank reactors

2016 ◽  
Vol 14 (3) ◽  
pp. 557-561
Author(s):  
Nguyễn Thị Yên ◽  
Kiều Thị Quỳnh Hoa
Keyword(s):  
Sulfate Reducing Bacteria ◽  
Stirred Tank ◽  
Synthetic Wastewater ◽  
Terminal Electron Acceptor ◽  
Stirred Tank Reactors ◽  
Sulfate Reducing ◽  
Sulfide Production ◽  
Continuous Stirred Tank ◽  
Continuous Stirred Tank Reactors ◽  
Reducing Bacteria

Lead contaminated wastewater negatively impacts to living organisms as well as humans. In recent years, a highly promising biological process using the anaerobic production of sulfide ions by sulfate-reducing bacteria has presented itself as an alternative option for the removal of lead. This process is based on microbial utilization of electron donors, such as organic compounds (carbon sources), and sulfate as the terminal electron acceptor for sulfide production. The biogenic hydrogen sulfide reacts with dissolved heavy metals to form insoluble metal sulfide precipitates Removal of lead by an enriched consortium of sulfate-reducing bacteria (DM10) was evaluated sulfate reduction, sulfide production and lead precipitation. Four parallel anaerobic continuous stirred tank reactors (CSTR, V = 2L) (referred as R1 - R4) were fed with synthetic wastewater containing Pb2+ in the concentrations of 0, 100, 150 and 200 mg L-1 of lead and operated with a hydraulic retention time of 5 days for 40 days. The loading rates of each metal in R1- R4 were 0, 20, 30 and 40 mg L-1 d-1, respectively. The results showed that there was no inhibition of SRB growth and that lead removal efficiencies of 99-100% for Pb2+ were achieved in R2 (100 mg L-1) and R3 (150 mg L-1) throughout the experiment. For the highest lead concentration of  200 mg L-1, a decrease in efficiency of removal (from 100 to 96%) was observed at the end of the experiment. The obtained result of this study might help for a better control operation and performance improvements of reactors.

EVALUATION OF FINANCIAL SECURITY OF THE REPUBLIC OF CRIMEA

2020 ◽  
pp. 56-67
Author(s):  
Elena Evgenevna Mashyanova ◽  
Elena Aleksandrovna Smirnova
Keyword(s):  
Financial Markets ◽  
Negative Impact ◽  
Gradual Increase ◽  
Financial System ◽  
Financial Security ◽  
Key Factors ◽  
Factors Affecting ◽  
External Threats ◽  
The Republic ◽  
Global Financial System

In modern conditions of development, financial security is an integral part of the overall security of the region and is formed on the basis of the functioning of the financial system. The complication of relationships between key segments of international financial markets, as well as the limited ability to accurately predict future trends in the development of the global financial system, lead to a gradual increase in the risks that accompany the activities of economic entities, and an increase in the number and scale of internal and external threats that have a negative impact on the financial security of the state. This formulation of the issue requires generalization of approaches to determining the financial security of the region in order to further formalize this issue and determine the key factors affecting it. The article considers the types of financial security, as well as certain areas of ensuring the financial security of the region and their priority. In work the assessment of the level of socio-economic development of the region with a view to ensuring financial security on the basis of which offers the main activities and priority areas of implementation of the investment policy that will ensure financial security of the Republic of Crimea.

scholarly journals Comparison of the sensitivity of Western blotting between PVDF and NC membranes

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yufang Xiang ◽  
Yuanyuan Zheng ◽  
Shaobo Liu ◽  
Gang Liu ◽  
Zhi Li ◽  
...  
Keyword(s):  
Western Blotting ◽  
Polyvinylidene Fluoride ◽  
Staining Intensity ◽  
Detection Sensitivity ◽  
Key Factors ◽  
Post Translational Modifications ◽  
Factors Affecting ◽  
Molecular Weights ◽  
Direct Blue ◽  
The Relationship

AbstractWestern blotting (WB) is one of the most widely used techniques to identify proteins as well as post translational modifications of proteins. The selection of electroblotted membrane is one of the key factors affecting the detection sensitivity of the protein which is transferred from gel to membrane in WB. The most common used membranes are polyvinylidene fluoride (PVDF) and nitrocellulose (NC) membranes. Which membrane of these two is more suitable for WB has not been reported so far. Here, by incubating proteins which were transferred to PVDF or NC membranes with a series of antibodies and different types of lectins, we investigated the relationship between the binding ability of these two membranes to proteins or glycoproteins and the molecular weight of the target protein. The antibody re-probed ability of the two membranes was also explored. Moreover, we verified the above results by directly incubating proteins having different molecular weights onto PVDF or NC membranes. Bound proteins were stained with direct blue-71, and the staining intensity was quantitated by scanning and densitometry.

scholarly journals The Effects of Mobility as a Service and Autonomous Vehicles on People’s Willingness to Own a Car in the Future

2021 ◽  
Vol 13 (4) ◽  
pp. 1962
Author(s):  
Timo Liljamo ◽  
Heikki Liimatainen ◽  
Markus Pöllänen ◽  
Riku Viri
Keyword(s):  
Public Transport ◽  
Autonomous Vehicles ◽  
Travel Behaviour ◽  
Car Ownership ◽  
Key Factors ◽  
Sustainable Mobility ◽  
Factors Affecting ◽  
Mobility Service ◽  
The Future ◽  
Mobility Behaviour

Car ownership is one of the key factors affecting travel behaviour and thus also essential in terms of sustainable mobility. This study examines car ownership and how people’s willingness to own a car may change in the future, when considering the effects of public transport, Mobility as a Service (MaaS) and automated vehicles (AVs). Results of two citizen surveys conducted with representative samples (NAV-survey = 2036; NMaaS-survey = 1176) of Finns aged 18–64 are presented. The results show that 39% of respondents would not want or need to own a car if public transport connections were good enough, 58% if the described mobility service was available and 65% if all vehicles in traffic were automated. Hence, car ownership can decrease as a result of the implementation of AVs and MaaS, and higher public transport quality of service. Current mobility behaviour has a strong correlation to car ownership, as respondents who use public transport frequently feel less of a will or need to own a car than others. Generally, women and younger people feel less of a will or need to own a car, but factors such as educational level and residential location seem to have a relatively low effect.

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