Increasing Yield and Antioxidative Performance of Litchi Pericarp Procyanidins in Baked Food by Ultrasound-Assisted Extraction Coupled with Enzymatic Treatment

Extraction with organic solvents is a traditional method to isolate bioactive compounds, which is energy-wasting and time-consuming. Therefore, enzyme and ultrasound treatments were combined to assist the extraction of oligomeric procyanidins from litchi pericarp (LPOPC), as an innovative approach to replace conventional extraction methods. Under optimum conditions (enzyme concentration 0.12 mg/mL, ultrasonic power 300 W, ultrasonic time 80 min, and liquid/solid ratio 10 mL/g), the yield of LPOPC could be improved up to 13.5%. HPLC analysis indicated that the oligomeric procyanidins (OPC) content of LPOPC from proposed extraction was up to 89.6%, mainly including (−)-epicatechin, procyanidin A1, A2, and A-type procyanidin trimer. Moreover, LPOPC powder was added in baked food to inhibit the lipid peroxidation. It was found that 0.2% (w/w) of LPOPC could maintain the quality of cookies in the first 7 days, by decreasing the peroxide values. The procyanidin dimers and trimers in LPOPC played more important roles as antioxidants compared to monomers during storage. The results also showed that the combined extraction process can be considered as a useful and efficient method for the extraction of functional components from other plant sources.

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Antioxidant Capacity and Antigenotoxic Effect of Hibiscus sabdariffa L. Extracts Obtained with Ultrasound-Assisted Extraction Process

Applied Sciences ◽

10.3390/app10020560 ◽

2020 ◽

Vol 10 (2) ◽

pp. 560

Author(s):

Gregorio Iván Peredo Pozos ◽

Mario Alberto Ruiz-López ◽

Juan Francisco Zamora Nátera ◽

Carlos Álvarez Moya ◽

Lucia Barrientos Ramírez ◽

...

Keyword(s):

Antioxidant Activity ◽

Human Lymphocytes ◽

Dry Weight ◽

Extraction Process ◽

Hibiscus Sabdariffa ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasound Assisted ◽

Antigenotoxic Effect

Hibiscus sabdariffa (Roselle) is in high demand worldwide due to its beneficial health properties owing to the polyphenols content, mainly in the flower calyx. The objective of this study was to find the best conditions (time and liquid: solid ratio) to extract polyphenols from Roselle using Ultrasound-Assisted Extraction (UAE) (40 kHz, 180 W), with ethanol how solvent; as well as determine the yield of phenols, anthocyanin, flavonoids, tannins, antioxidant activity (DPPH) and antigenotoxic effect (comet assay). A traditional solid-liquid extraction was applied as a reference. Extraction times of 40 and 60 min resulted in the highest polyphenols (13.019 mg GAE/g dry weight (dw)), flavonoids (4.981 CE/g dw), anthocyanins (1.855 mg Cya3GE/g dw), and tannins (0.745 CE/g dw) recoveries and an antioxidant activity (DPPH) of 74.58%. Extracts from white calyces contained similar amounts of phenols and flavonoids, but very little condensed tannins (0.049 CE/g dw) and practically no anthocyanins. Extracts from red and white calyces, showed antigenotoxic activity and repaired capacity of damage caused by mutagens in human lymphocytes.

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Optimization of Ultrasound-Assisted Extraction of Oil from Camellia(Camellia oleifera abel) Seed

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.236-238.1854 ◽

2011 ◽

Vol 236-238 ◽

pp. 1854-1858 ◽

Cited By ~ 2

Author(s):

Xue Hui Wu ◽

Li Li

Keyword(s):

Ultrasonic Treatment ◽

Treatment Time ◽

Treatment Temperature ◽

Ultrasonic Power ◽

Ultrasound Assisted Extraction ◽

Camellia Oil ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasound Assisted ◽

Main Component

Camellia oil is the main component of the camellia seed and has many beneficial functions to human health. An investigation into ultrasound- assisted extraction was conducted for the oil extraction from camellia seed. The optimized condition of extraction parameters obtained with the response surface methodology (RSM) at a four-variable, three-level experiment Box–Behnken design (BBD), was as follows: liquid-solid ratio, 5mL/g;ultrasonic power,155W;ultrasonic treatment time,31 min; and ultrasonic treatment temperature, 55°C, under these conditions, the oil yeild was 44.55%. GC–MS analysis showed that the content of unsaturated fatty acid in camellia oil was up to 84.47% and the content of oleic acid was over 76.15%.

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Ultrasound-Assisted Enzymatic Extraction of Adenosine from Vietnamese Cordyceps militaris and Bioactivity Analysis of the Extract

Journal of Chemistry ◽

10.1155/2020/1487654 ◽

2020 ◽

Vol 2020 ◽

pp. 1-10

Author(s):

Thanh Vuong Hoai ◽

Phuc Nguyen Cao ◽

My Phan Le Thao ◽

Tran Dat Do ◽

Nam Hoang Minh ◽

...

Keyword(s):

Cordyceps Militaris ◽

Ultrasonic Power ◽

Enzymatic Extraction ◽

Ultrasound Assisted Extraction ◽

Chromatography Method ◽

Isolation And Purification ◽

Assisted Extraction ◽

Ultrasonic Time ◽

Power Ultrasonic ◽

Ultrasound Assisted

Vietnamese Cordyceps militaris (C. militaris) has long been recognized as one of the most valuable traditional Chinese medicines. In this study, adenosine was extracted from Vietnamese C. militaris by ultrasound-assisted enzymatic extraction method (UAEE) using water as a solvent. Then, the effects of five single factors on adenosine content including pH, enzyme-to-material ratio, ultrasonic power, ultrasonic time, and ultrasonic temperature were determined. After that, three factors consisting of ultrasonic power, ultrasonic time, and ultrasonic temperature were chosen based on their effects on adenosine content. The simultaneous influence of these factors on the adenosine content was investigated by response surface method using central composite design. The adenosine content was evaluated by high-performance liquid chromatography method. Under the optimal conditions, the extract was evaluated for antioxidant and anticancer bioactivities. In addition, different extraction methods including aqueous extraction (AE), ultrasound-assisted extraction (UAE), and enzyme-assisted extraction (EAE) methods were carried out to compare with UAEE. As a result, it can be concluded that UAEE is a promising method for adenosine extraction and further studies regarding isolation and purification need to be conducted.

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Optimization of ultrasound-assisted extraction of polyphenolic compounds from coriander seeds using response surface methodology

Acta periodica technologica ◽

10.2298/apt1647249z ◽

2016 ◽

pp. 249-263 ◽

Cited By ~ 1

Author(s):

Zoran Zekovic ◽

Sasa Djurovic ◽

Branimir Pavlic

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Three Dimensional ◽

Extraction Process ◽

Biologically Active ◽

Ultrasonic Power ◽

Ultrasound Assisted Extraction ◽

Assisted Extraction ◽

Order Polynomial ◽

Predicted Values

Coriandrum sativum L. (coriander) seeds (CS) were used for preparation of extracts with high content of biologically active compounds. In order to optimize ultrasoundassisted extraction process, three levels and three variables of Box-Behnken experimental design (BBD) in combination with response surface methodology (RSM) were applied, yielding maximized total phenolics (TP) and flavonoids (TF) content and antioxidant activity (IC50 and EC50 values). Independent variables were temperature (40-80oC), extraction time (40-80 min) and ultrasonic power (96-216 W). Experimental results were fitted to a second-order polynomial model with multiple regression, while the analysis of variance (ANOVA) was employed to assess the model fitness and determine optimal conditions for TP (79.60oC, 49.20 min, 96.69 W), TF (79.40oC, 43.60 min, 216.00 W), IC50 (80.00oC, 60.40 min, 216.00 W) and EC50 (78.40oC, 68.60 min, 214.80 W). On the basis of the obtained mathematical models, three-dimensional surface plots were generated. The predicted values for TP, TF, IC50 and EC50 were: 382.68 mg GAE/100 g CS, 216 mg CE/100 g CS, 0.03764 mg/mL and 0.1425 mg/mL, respectively.

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A ULTRASOUND-ASSISTED EXTRACTION (UAE) OF PHYTOCHEMICALS WITH RESPONSE SURFACE METHODOLOGY(RSM) IN Curcuma xanthorrhiza

Science Proceedings Series ◽

10.31580/sps.v2i2.1431 ◽

2020 ◽

Vol 2 (2) ◽

pp. 139-146

Author(s):

Najiba Azemi ◽

Norazah Basar ◽

Susilawati Toemen

Keyword(s):

Response Surface Methodology ◽

Optimum Condition ◽

Response Surface ◽

Extraction Process ◽

Extraction Time ◽

Array Detector ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasound Assisted

The Curcuma genus belongs to the Zingiberaceae family is commonly known for its usage as spice and medicine.it is widely used in pharmaceutical, food supplement, cosmetic industries lead to economic benefits. Recently, noticing to an eco-friendly extraction approach which provides the extraction efficiency is considered that in the extraction process, exploring the optimum condition of extraction parameters. This study is performed to improve the bioactive compounds extraction from C. xanthorrhiza by ultrasound-assisted extraction (UAE) through Response Surface Methodology (RSM). Three independent variables: extraction time (5-20 min), temperature (30-50 ° C) and liquid-solid ratio (6-10 mL / g) are applied using Box-Behnken (BBD) design. Quantification of curcumin and xanthorrhizol are carried out using reversed-phase high-performance liquid chromatography (RP HPLC) equiped by a photodiode array detector. Extraction time 20 minutes, temperature of 50°C, and LS ratio 8 mL/g are found as the optimum condition of extraction yield and quantification of xanthorrhizol. Curcumin 's optimum state, however, is observed at extraction time 12.50 minutes, temperature 30 ° C, and liquid-solid ratio of 10 mL / g. This study suggests that there are favorable potential using method of ultrasound-assisted extraction under specific parameters in the extraction process which is advantageous for advanced research.

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Ultrasonic-Assisted Extraction of Procyanidins Using Ionic Liquid Solution fromLarix gmeliniiBark

Journal of Chemistry ◽

10.1155/2013/541037 ◽

2013 ◽

Vol 2013 ◽

pp. 1-9 ◽

Cited By ~ 8

Author(s):

Xiaowei Sun ◽

Zhimin Jin ◽

Lei Yang ◽

Jingwei Hao ◽

Yuangang Zu ◽

...

Keyword(s):

Ionic Liquid ◽

Ionic Liquids ◽

Extraction Efficiency ◽

Liquid Solution ◽

Extraction Process ◽

Ultrasonic Power ◽

Assisted Extraction ◽

Ultrasonic Time ◽

Ultrasonic Assisted ◽

Ultrasonic Assisted Extraction

An ionic liquid-based ultrasonic-assisted extraction method has been developed for the effective extraction of procyanidins fromLarix gmeliniibark. So as to evaluate the performance of ionic liquids in ultrasonic-assisted extraction process, the effects caused by changes in the anion and the alkyl chain length of the cation on the extraction efficiency were investigated in this paper. The results indicated that the characteristics of anions had remarkable effects on the extraction efficiency of procyanidins, and 1-butyl-3-methylimidazolium bromide ([Bmim]Br) aqueous solution was the best among the investigated ionic liquids. The optimum conditions for the extraction were as follows: [Bmim]Br concentration 1.25 M, soak time 3 h, solid-liquid ratio 1 : 10, ultrasonic power 150 W, and ultrasonic time 30 min. This work not only introduces a simple, green, and highly efficient sample preparation method for extraction of procyanidins fromL. gmeliniibark, but also reveals the tremendous application potential of ionic liquids.

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Optimizing the Conditions for Polysaccharides Ultrasonic-Assisted Extraction in Mycelium of Paecilomyces Tenuipes Pt196 Using Statistical Approach

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.756-759.180 ◽

2013 ◽

Vol 756-759 ◽

pp. 180-185

Author(s):

Lin Na Du ◽

Wen Ting Bu ◽

Wen Lin Pan ◽

Chen Shao ◽

Jia Hui Lu ◽

...

Keyword(s):

Neural Network ◽

Genetic Algorithm ◽

Artificial Neural Network ◽

Ultrasonic Power ◽

Box Behnken Design ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasonic Time ◽

Ultrasonic Assisted ◽

Artificial Neural

In order to enhance the extraction rate of polysaccharides, a series of statistical approach was used to optimize the ultrasonic-assisted extraction conditions from the mycelium of Paecilomyces tenuipes Pt196. [Metho The optimization of conditions was carried out in two stages. Firstly, the effects of various experimental parameters considered for the investigation (ultrasonic power, ultrasonic time and liquid-solid ratio) were studied using the method of single factor test design experiments. Secondly, a 15-run Box-Behnken design was performed to optimize the extraction conditions of polysaccharides. The experimental results of Box-Behnken design were analyzed by response surface methodology and artificial neural network together with genetic algorithm. [Result The optimum conditions for polysaccharides extraction obtained by the application of artificial neural network-genetic algorithm were ultrasonic time 345s, ultrasonic power 320 W and liquid-solid ratio 95 mL·g-1. [Conclusion Artificial neural network-genetic algorithm can effectively select the best extraction conditions of polysaccharides in this study.

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Study on Extraction Process of Technics of Litsea Cubeba Oil

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.602-605.81 ◽

2014 ◽

Vol 602-605 ◽

pp. 81-85 ◽

Cited By ~ 1

Author(s):

Zuo Ren Liu ◽

Yan Fen Zhang ◽

Yong Guang Bi ◽

Hai Ling Li

Keyword(s):

Visual Analysis ◽

Extraction Process ◽

Oil Extraction ◽

Ultrasonic Power ◽

Extraction Temperature ◽

Litsea Cubeba ◽

Assisted Extraction ◽

Ultrasonic Time ◽

Ultrasonic Assisted ◽

Ultrasonic Assisted Extraction

Litsea cubeba fruit as raw material in this experiment, the use of ultrasonic-assisted method of litsea cubeba oil extraction process are discussed. The effects of several ultrasonic processing parameters, such as ratio of liquid to solid, extraction temperature, ultrasonic power and extraction time on the extraction of litsea cubeba oil were investigated assisted by ultrasonic wave. And the L9 (34) orthogonal test was used to research this four factors, the result from the visual analysis indicated that the optimal extraction process was gotten when ratio of liquid to solid was 14:1 , ultrasonic time was 50min, ultrasonic power is 320W, ultrasonic temperature is 60 °C.Those experimental results showed that ultrasonic-assisted extraction of the Litsea cubeba oil is practicable, and this method is simple and efficient. With the preliminary investigation of the ultrasonic-assisted extraction of Litsea Cubeba oil, the oil yield reached up to 41.35%, this value is the highest of all the oil yields from the current oil extraction methods, and it is worthy of further exploration .

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Applied Orthogonal Experiment Design for Optimization of Ultrasonic-Assisted Extraction of Polysaccharides from Agaricus bisporus Mycelia

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.1010-1012.1779 ◽

2014 ◽

Vol 1010-1012 ◽

pp. 1779-1782 ◽

Cited By ~ 4

Author(s):

Quan Sheng Chen ◽

Tao Ying Zhou ◽

Xing Yuan Li ◽

Yu Bi ◽

Hui De Yang

Keyword(s):

Agaricus Bisporus ◽

Orthogonal Experiment ◽

Experiment Design ◽

Ultrasonic Power ◽

Extraction Temperature ◽

Orthogonal Experiment Design ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasonic Time ◽

Extraction Parameters

The purpose of this study to establish the optimum ultrasound-assisted extraction (UAE) parameters of Polysaccharides from Agaricus bisporus mycelia (PABM). Single factor experiment was employed to determine the preliminary range of the extraction parameters including liquid-solid ratio, ultrasonic time, extraction temperature, and ultrasonic power. Then, an orthogonal experiment design was employed to determine the best extraction parameters of PABM. the results showed that the optimal UAE parameters of PABM was liquid-solid ratio of 40 ml/g, ultrasonic time of 70 min, extraction temperature of 55°C, and ultrasonic power of 660 W.

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Ultrasound-Assisted Extraction Optimization of α-Glucosidase Inhibitors from Ceratophyllum demersum L. and Identification of Phytochemical Profiling by HPLC-QTOF-MS/MS

Molecules ◽

10.3390/molecules25194507 ◽

2020 ◽

Vol 25 (19) ◽

pp. 4507

Author(s):

Zhen Li ◽

Zongcai Tu ◽

Hui Wang ◽

Lu Zhang

Keyword(s):

Chinese Herb ◽

Ceratophyllum Demersum ◽

Ultrasonic Power ◽

Extraction Temperature ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Glucosidase Inhibitors ◽

Assisted Extraction ◽

Ic50 Value ◽

Ultrasound Assisted

Ceratophyllum demersum L. (CDL) is a traditional Chinese herb to treat many diseases, but research on its anti-diabetic activity is not available. In this research, the α-glucosidase inhibitory ability and phytochemical constituents of CDL extract were firstly studied. Optimal ultrasound-assisted extraction conditions for α-glucosidase inhibitors (AGIs) were optimized by single factor experiment and response surface methodology (RSM), which was confirmed as 70% methanol, liquid-to-solid ratio of 43 (mL/g), extraction time of 54 min, ultrasonic power of 350 W, and extraction temperature of 40 °C. The lowest IC50 value for α-glucosidase inhibition was 0.15 mg dried material/mL (mg DM/mL), which was much lower than that of acarbose (IC50 value of 0.64 mg DM/mL). In total, 80 compounds including 8 organic acids, 11 phenolic acids, 25 flavonoids, 21 fatty acids, and 15 others were identified or tentatively identified from CDL extract by HPLC-QTOF-MS/MS analysis. The results suggested that CDL could be a potential source of α-glucosidase inhibitors. It can also provide useful phytochemical information for research into other bioactivities.

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