Stability Analysis of Anthocyanins Using Alcoholic Extracts from Black Carrot (Daucus Carota ssp. Sativus Var. Atrorubens Alef.)

Anthocyanins are used for food coloring due their low toxicity and health benefits. They are extracted from different sources, but black carrot has higher anthocyanin content compared with common fruits and vegetables. Here, we study alcoholic anthocyanin extracts from black carrot to enhance their stability. The objective of our research is to determine if microencapsulation with tetraethyl orthosilicate (TEOS) is a feasible option for preventing black carrot anthocyanin degradation. Extraction solvents were solutions of (1) ethanol/acetic acid and (2) ethanol/citric acid. Samples were purified through a resin column and microencapsulated using TEOS. Fourier Transformed Infrared Spectroscopy (FTIR) spectra of samples were obtained, and degradation studies were performed under different conditions of UV radiation, pH and temperature. Antioxidant activity was evaluated with radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging and electrochemical cupric reducing antioxidant capacity (CUPRAC). Color evaluation on food models were performed with CIE Lab at the beginning of experiments and after 25 days of storage. Results indicate that the more stable extracts against pH media changes are samples obtained with ethanol/acetic acid solution as extraction solvent. Extract purification through resin and TEOS microencapsulation had no significant effect on extract stability. In conclusion, although TEOS microencapsulation has proven to be effective for some dried materials from natural extracts in our previous research, we do not recommend its use for black carrot extracts considering our results in this particular case.

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Preparation of Non-Acylated and Acylated Anthocyanins from Black Carrot (Daucus carota L.) for Use as Food Ingredient

10.20944/preprints201806.0288.v1 ◽

2018 ◽

Author(s):

Guillermo Espinoza-Acosta ◽

Ana L. Ramos-Jacques ◽

Jose Maya-Cornejo ◽

Gustavo A. Molina ◽

Rodrigo Esparza ◽

...

Keyword(s):

Citric Acid ◽

Fruits And Vegetables ◽

Packed Column ◽

Anthocyanin Content ◽

Food Ingredient ◽

Black Carrot ◽

Daucus Carota L ◽

Ethanol Acetic Acid ◽

Dpph Scavenging ◽

Cie Lab

Anthocyanins are used for food coloring due to their low toxicity and health benefits. They are extracted from different sources, but black carrot has higher anthocyanin content compared with common fruits and vegetables. Here, we study anthocyanin extracts from black carrot to enhance their performance as food ingredient. Extracts were obtained by ethanol/citric acid and ethanol/acetic acid. Samples were purified through to a packed column and microencapsulated using tetraethylorthosilicate (TEOS). Degradation studies were performed by UV radiation, and pH and temperature media changes. Antioxidant activity was evaluated with radical DPPH scavenging and cupric reducing antioxidant capacity (CUPRAC). Color evaluations were performed with CIE Lab at the beginning and after 25 days of storage. Samples extracted with ethanol/citric acid had higher anthocyanin content. After purification, there was evidence that 3-glucoside cyanidins were trapped into resin. Results indicate that the purest acetylated 3-glucoside derivatives are more stable than the citric acid non-purified extraction. The antiradical activity of black carrots extracts was higher than other reported values, when anthocyanin content is higher, antioxidant effect increases. In conclusion, acylation of anthocyanins provides them more stability against pH media changes, purification with resin and TEOS microencapsulation not necessary benefit stability of the black carrot extracts.

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Preparation of Coated Corrugated Box for Controlled-Release of Chlorine Dioxide and Its Application in Strawberry Preservation

Coatings ◽

10.3390/coatings10030242 ◽

2020 ◽

Vol 10 (3) ◽

pp. 242 ◽

Cited By ~ 1

Author(s):

Yuanyuan Li ◽

Dan Ren ◽

Dan Xu

Keyword(s):

Weight Loss ◽

Acetic Acid ◽

Water Vapor ◽

Chlorine Dioxide ◽

Room Temperature ◽

Fruits And Vegetables ◽

Acetic Acid Solution ◽

High Humidity ◽

Surface Color ◽

Inner Surface

Chlorine dioxide (ClO2) has received great attention as a nontoxic and efficient antimicrobial agent for the preservation of fresh fruits and vegetables. A novel two-layer coated corrugated box was developed to release gaseous ClO2 under the trigger of moisture in this study. The inner surface of the box was firstly coated with a mixture of polyvinyl alcohol-NaClO2-diatomite and then with chitosan acetic acid solution. Results showed that ClO2 was successfully released under high humidity due to the reaction of NaClO2, water vapor and acid. The concentration of released ClO2 increased with the increasing NaClO2 content in the coating, while the addition of diatomite stabilized and extended the release. To evaluate the preservation effect, strawberries were packed in the coated box and stored at room temperature. Compared with the control, the decay rate and weight loss of the strawberries packed in the coated box (9 g/L NaClO2) were reduced up to 21.88% and 6.84%, respectively. The surface color, firmness and nutrients content were also better maintained. Therefore, this coated corrugated box with the capability to release ClO2 under the trigger of moisture has great potential to be applied as an antimicrobial packaging for fresh fruits and vegetables.

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Facile Synthesis of Ultrafine Hematite Nanowire Arrays in Mixed Water–Ethanol–Acetic Acid Solution for Enhanced Charge Transport and Separation

ACS Applied Materials & Interfaces ◽

10.1021/acsami.7b18534 ◽

2018 ◽

Vol 10 (15) ◽

pp. 12594-12602 ◽

Cited By ~ 8

Author(s):

Jian Wang ◽

Menglong Wang ◽

Tao Zhang ◽

Zhiqiang Wang ◽

Penghui Guo ◽

...

Keyword(s):

Acetic Acid ◽

Acid Solution ◽

Charge Transport ◽

Acetic Acid Solution ◽

Nanowire Arrays ◽

Facile Synthesis ◽

Ethanol Acetic Acid ◽

Mixed Water

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Bioactive Compounds and Antioxidant Capacity in Anthocyanin-Rich Carrots: A Comparison between the Black Carrot and the Apulian Landrace “Polignano” Carrot

Plants ◽

10.3390/plants10030564 ◽

2021 ◽

Vol 10 (3) ◽

pp. 564

Author(s):

Federica Blando ◽

Stefano Marchello ◽

Gabriele Maiorano ◽

Miriana Durante ◽

Angelo Signore ◽

...

Keyword(s):

Antioxidant Capacity ◽

Bioactive Compounds ◽

Fruits And Vegetables ◽

Anthocyanin Content ◽

Total Anthocyanin ◽

Black Carrot ◽

Total Anthocyanin Content ◽

Acylated Anthocyanins ◽

High Level ◽

Β Carotene

The carrot is one of the most cultivated vegetables in the world. Black or purple carrots contain acylated anthocyanins which are of special interest to the food industry for their stability and nutraceutical characteristics. Anthocyanin-rich fruits and vegetables have gained popularity in the last ten years, due to the health benefits they provide. In this paper, the characterizations of the bioactive compounds and antioxidant capacities of different anthocyanin-containing carrots (a black carrot—BC, and a local purple carrot, the “Polignano” carrot—PC), compared to the commercial orange carrot (OC) (lacking of anthocyanins), are reported. The anthocyanin profiles of the polyphenolic extracts of BC and PC were similar, but differences were observed at quantitative levels. The total anthocyanin content in BC was more than twice that in PC (13.84 ± 0.61 vs. 5.64 ± 0.48 mg K Eq. g−1 DW). Phenolic acids (mostly chlorogenic acid) were also present at high level in anthocyanin-rich carrots compared to OC. High polyphenol content accounted also for a high reducing capacity (evaluated by Folin–Ciocalteu reagent, FCR), and antioxidant capacity (evaluated by TEAC and ORAC assays) which were the highest for BC (FCR value: 16.6 ± 1.1 mg GAE. g−1 DW; TEAC: 76.6 ± 10.6 µmol TE. g−1 DW; ORAC: 159.9 ± 3.3 µmol TE. g−1 DW). All carrot genotypes (mostly OC) were rich in carotenoids (BC 0.14 ± 0.024; PC 0.33 ± 0.038; OC 1.29 ± 0.09 mg. g−1 DW), with predominance of α and β-carotene, in OC, and lutein in BC. PC showed the highest malic acid and sugar (glucose plus fructose) content. In conclusion, while BC is remarkable for nutraceutical features, the local genotype (“Polignano” carrot) is worth considering in genetic biodiversity conservation programme.

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Some Approaches to Viscometric Study of Chitosan in Acetic Acid Solution

Chemistry & Chemical Technology ◽

10.23939/chcht10.02.135 ◽

2016 ◽

Vol 10 (2) ◽

pp. 135-139 ◽

Cited By ~ 3

Author(s):

Valentina Chernova ◽

◽

Angela Shurshina ◽

Elena Kulish ◽

Gennady Zaikov ◽

...

Keyword(s):

Acetic Acid ◽

Acid Solution ◽

Intrinsic Viscosity ◽

Acetic Acid Solution ◽

Conformational State ◽

Macromolecular Coil ◽

Degree Of Swelling ◽

Current Value ◽

Viscometric Study

Some ways of estimating the values of the intrinsic viscosity of chitosan were analyzed. It was shown that the method of Irzhak and Baranov for estimating the current value of the intrinsic viscosity allows to adequately estimates the conformational state of the macromolecular coil and its degree of swelling.

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HPLC-UV Method for Determination of Famotidine from Pharmaceutical Products

Revista de Chimie ◽

10.37358/rc.18.2.6093 ◽

2018 ◽

Vol 69 (2) ◽

pp. 297-299

Author(s):

Adriana Nita ◽

Delia Mirela Tit ◽

Lucian Copolovici ◽

Carmen Elena Melinte (Frunzulica) ◽

Dana Maria Copolovici ◽

...

Keyword(s):

Acetic Acid ◽

Quantitative Analysis ◽

Linear Response ◽

Mobile Phase ◽

Acetic Acid Solution ◽

Pharmaceutical Products ◽

Quantification Limit ◽

Validation Parameters ◽

Hplc Analyses

The aim of this study was to develop and validate a rapid, accurate, and exact method for the quantitative determination of famotidine in pharmaceutical products. The HPLC analyses were performed by using a mobile phase containing methanol:1% acetic acid solution=30:7 (v/v), at a flow rate of 0.4 mL/min.The total time of the method was 10 min, and the retention time of famotidine was 4.16 min. The detection was evaluated at l=267 nm. The method has been validated by using different validation parameters. The linear response of the detector for famotidine peak area was observed at concentrations ranging from 0.1 to 0.0001 mg mL-1 , resulting in a correlation coefficient of 0.99998. The values of the detection limit and of the quantification limit are 0.00048 mg mL-1 and 0.00148 mg mL-1, respectively. The method proposed allowed accurate (with a relative error of less than 2%) and precise (RSD values less than 2.0%) determination of famotidine content in pharmaceutical products and can be used for its rapid quantitative analysis.

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Study on the initiation mechanism upon oxidation of methylaromatic hydrocarbons in presence of cobalt acetate and sodium bromide in acetic acid solution

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19752084 ◽

1975 ◽

Vol 40 (7) ◽

pp. 2084-2092 ◽

Cited By ~ 2

Author(s):

S. Ivanov ◽

M. Hinkova

Keyword(s):

Acetic Acid ◽

Acid Solution ◽

Acetic Acid Solution ◽

Cobalt Acetate ◽

Sodium Bromide ◽

Initiation Mechanism

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Durability of Red Mud Based Geopolymer Paste in Acid Solutions

Materials Science Forum ◽

10.4028/www.scientific.net/msf.866.99 ◽

2016 ◽

Vol 866 ◽

pp. 99-105 ◽

Cited By ~ 3

Author(s):

Smita Singh ◽

M.U. Aswath ◽

R.V. Ranganath

Keyword(s):

Mechanical Properties ◽

Acetic Acid ◽

Fly Ash ◽

Red Mud ◽

Acetic Acid Solution ◽

Strength Loss ◽

Acid Solutions ◽

Test Results ◽

Acid Attack ◽

Geopolymer Paste

The present investigation is on the effect of red mud on the mechanical properties and durability of the geopolymer paste in sulphuric and acetic acid solution. Red mud and fly ash were used to form the geopolymer paste along with the alkalies. The variation of red mud in the paste composition was from 0% to 90%. Cylindrical shaped specimens of 1 inch diameter and 1 inch height were prepared. The specimens were immersed in 5% sulphuric acid and 5% acetic acid for 1, 7, 14, 28, 56 and 84 days and tested for weight loss, visual deformation, strength loss and colour of the solvent, based on the procedure specified by ASTM C 267 – 01. SEM/EDX Tests were performed on the geopolymer specimens. Test results show that initially, the strength of the geopolymer increased upon the addition of red mud. The strength was maximum when the percentage of red mud was 30%. The maximum strength obtained was 38 MPa for the paste containing 30% red mud using 10M alkali solution as against 31.69 MPa, when only fly ash was used. Geopolymer paste containing 30% and 50% red mud showed better resistance to acid attack. The strength loss was minimum for the samples containing 30% red mud in both inorganic and organic acid i.e. sulphuric and acetic acid.

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