Evaluation of Antioxidant Capacity, Protective Effect on Human Erythrocytes and Phenolic Compound Identification in Two Varieties of Plum Fruit (Spondias spp.) by UPLC-MS

Plum edible part was used to obtained extracts by during a 4 h maceration process using three different solvents (ethanol, methanol and water) for the determination of total phenols and flavonoids, antioxidant capacity by (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hemolysis inhibition in human blood assays. Subsequently, phenolic compounds were identified using ultra-performance liquid chromatography (UPLC-MS). The results indicated that the ethanolic extract of plum fruit being a good source of phenolic (12–18 mg GAE/g FW) and flavonoids (2.3–2.5 mg QE/g FW) content in both varieties of plum. Also, the fruits proved a good source of antioxidants as measured by DPPH and ABTS; likewise, plum aqueous extracts showed the highest protective effect on human erythrocytes with 74.34 and 64.62% for yellow and red plum, respectively. A total of 23 bioactive compounds were identified by UPLC-MS, including gallic acid, rutin, resorcinol, chlorogenic acid, catechin, and ellagic acid, and the antioxidant capacity can be attributed to these species. The edible part of plum contains compounds of biological interest, suggesting that this fruit has antioxidant potential that can be exploited for various technologies.

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Canjiqueira Fruit: Are We Losing the Best of It?

Foods ◽

10.3390/foods9040521 ◽

2020 ◽

Vol 9 (4) ◽

pp. 521

Author(s):

Daniela G. Arakaki ◽

Vanessa Samúdio dos Santos ◽

Elaine Pádua de Melo ◽

Hugo Pereira ◽

Priscila Silva Figueiredo ◽

...

Keyword(s):

Thermal Stability ◽

Seed Oil ◽

Food Resource ◽

Bioactive Compound ◽

Icp Oes ◽

Compound Identification ◽

Good Source ◽

Elemental Characterization ◽

Thermal Stability Of

Fruits and byproducts are valuable sources of nutrients and bioactive compounds, which are associated with a decreased risk of developing several diseases, such as cancer, inflammation, cardiovascular diseases, and Alzheimer’s. The fruits of canjiqueira (Byrsonima cydoniifolia) are already exploited as a food resource, while the seeds are discarded. This study aimed at showing the potential of the whole fruit of canjiqueira. Elemental characterization was performed on ICP OES, while thermal stability was assessed on thermogravimetry. The determination of the fatty acid profile was carried out on gas chromatography and bioactive compound identification using liquid chromatography and mass spectrometry. Results show that both parts of canjiqueira fruit are a source of various minerals, such as Ca, Cu, Fe, K, Mg, and Mn while the seed only is a good source for Zn. Oleic and linoleic acids are the main compounds in pulp and seed. The thermal stability of seed oil is superior to pulp oil, while piceatannol concentration is higher in seed than pulp. All parts of canjiqueira fruit may be used as a strategy to address nutrition issues and are valuable ingredients to prospective food products.

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Determination of Polyphenolic Compounds by Ultra-Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry and Antioxidant Capacity of Spanish Subtropical Fruits

Agricultural Sciences ◽

10.4236/as.2018.92014 ◽

2018 ◽

Vol 09 (02) ◽

pp. 180-199 ◽

Cited By ~ 4

Author(s):

Adelaida Esteban Muñoz ◽

Montserrat Barea Álvarez ◽

María-Jesús Oliveras-López ◽

Rafael Giménez Martínez ◽

José Ángel Rufián Henares ◽

...

Keyword(s):

Mass Spectrometry ◽

Liquid Chromatography ◽

Tandem Mass Spectrometry ◽

Antioxidant Capacity ◽

Ultra Performance Liquid Chromatography ◽

Polyphenolic Compounds ◽

Tandem Mass

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Antiviral, Antioxidant, and Antihemolytic Effect of Annona muricata L. Leaves Extracts

Plants ◽

10.3390/plants9121650 ◽

2020 ◽

Vol 9 (12) ◽

pp. 1650

Author(s):

Ana Paola Balderrama-Carmona ◽

Norma Patricia Silva-Beltrán ◽

Juan-Carlos Gálvez-Ruiz ◽

Saúl Ruíz-Cruz ◽

Cristóbal Chaidez-Quiroz ◽

...

Keyword(s):

Viral Infections ◽

Ethanolic Extract ◽

Ultra Performance Liquid Chromatography ◽

Human Erythrocytes ◽

Tropical Tree ◽

Protective Effects ◽

Annona Muricata ◽

Leaf Extracts ◽

Trolox Equivalent

Annona muricata L. is a tropical tree that is used in traditional medicine around the world. The high content of flavonoid, alkaloid, acetogenin, phenolic and lipophilic compounds of this tropical tree forms the basis of its traditional medical uses. Our objective was to study soursop leaf extracts to support their use as antiviral therapies and investigate their protective effects against oxidative damage. The aqueous extract (AE) and acidified ethanolic extract (AEE) of soursop leaves were characterized by ultra performance liquid chromatography (UPLC), and their effects on human erythrocytes and in vitro antioxidant capacity, as evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assays, were investigated. The antiviral effects were evaluated using a bacteriophage surrogate. AEE showed the highest phenolic content, with rutin as the predominant compound. This extract showed higher values in the DPPH and ABTS assays, with 23.61 ± 0.42 and 24.91 ± 0.16 mmol of Trolox equivalent per gram, respectively. Inhibition of hemolysis was 34% and 51% for AE and AEE, respectively. AEE was selected for the antiviral study because of its higher antioxidant activity. The viral reduction ranged from 5–6 log10 plaque-forming units/volume (PFU) at contact times of 15–360 min. Soursop leaves have a positive effect on reducing oxidative stress in human erythrocytes and viral infections.

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