Chemical Composition and Antimicrobial Activities of Artemisia argyi Lévl. et Vant Essential Oils Extracted by Simultaneous Distillation-Extraction, Subcritical Extraction and Hydrodistillation

Artemisia argyi Lévl. et Vant essential oil could be used as a good antimicrobial flavouring agent and applied in the food industry. In this study, three methods, including simultaneous distillation-extraction (SDE), subcritical extraction and hydrodistillation, were applied to extract A. argyi essential oil. Compared with subcritical extraction (1%) and hydrodistillation (0.5%), SDE gave a higher yield (1.2%). Components of the essential oils were analysed with gas chromatography-mass spectrometry (GC-MS), and the most abundant ingredients were caryophyllene oxide, neointermedeol, borneol, α-thujone and β-caryophyllene. These five components accounted for 82.93%, 40.90% and 40.33% for SDE, subcritical extraction, and hydrodistillation, respectively. Based on agar disc diffusion and minimum inhibitory concentration (MIC) assays, SDE oil showed a significant inhibitory effect towards Listeria monocytogenes, Escherichia coli, Proteus vulgaris, Salmonella enteritidis and Aspergillus niger. Furthermore, electron microscope observations (SEM) confirmed that SDE oil could obviously deform cell morphology and destroy the structure of cell walls. Performances showed that SDE was a promising process for extracting A. argyi essential oil with both high yield and antimicrobial activity.

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Chemical Composition and Antioxidant and Antimicrobial Activities of Wormwood (Artemisia absinthiumL.) Essential Oils and Phenolics

Journal of Chemistry ◽

10.1155/2015/804658 ◽

2015 ◽

Vol 2015 ◽

pp. 1-12 ◽

Cited By ~ 35

Author(s):

Kamel Msaada ◽

Nidhal Salem ◽

Olfa Bachrouch ◽

Slim Bousselmi ◽

Sonia Tammar ◽

...

Keyword(s):

Gas Chromatography ◽

Essential Oil ◽

Essential Oils ◽

Methanolic Extract ◽

Biological Activities ◽

Reducing Power ◽

Antimicrobial Activities ◽

Gas Chromatography Mass Spectrometry ◽

Oil Composition ◽

Antioxidant And Antimicrobial Activities

The aim of this study was to determine the chemical variability of wormwood extracts as affected by the growing region. Antioxidant and antimicrobial activities were also investigated. The essential oil composition variability ofA. absinthiumL. aerial parts collected from four different Tunisian regions was assessed by gas chromatography (GC/FID) and by gas chromatography mass spectrometry (GC/MS). In addition, total polyphenols, flavonoids, and condensed tannins as well as antioxidant, antibacterial, and antifungal activities of methanolic extract and essential oils were undertaken. Chromatographic analysis of wormwood essential oils showed the predominance of monoterpene hydrocarbons represented mainly by chamazulene. RP-HPLC analysis of wormwood methanolic extract revealed the predominance of phenolic acids. Antiradical activity was region-dependant and the methanolic extract of Bou Salem region has the strongest activity (CI50=9.38±0.82 µg/mL). Concerning the reducing power, the methanolic extract of Bou Salem, Jérissa, and Boukornine regions was more active than the positive control. Obtained results of antimicrobial activities showed that wormwood essential oil is endowed with important antibacterial activity which was strongly related to the organoleptic quality of oil which appeared strongly region-dependant.A. absinthiumL. EOs investigated are quite interesting from a pharmaceutical standpoint because of their biological activities.

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Volatile Compounds and Antioxidant and Antimicrobial Activities of Selected Citrus Essential Oils Originated from Nepal

Molecules ◽

10.3390/molecules26216683 ◽

2021 ◽

Vol 26 (21) ◽

pp. 6683

Author(s):

Devi Prasad Bhandari ◽

Darbin Kumar Poudel ◽

Prabodh Satyal ◽

Karan Khadayat ◽

Sital Dhami ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Citrus Sinensis ◽

Antioxidant Properties ◽

Antimicrobial Activities ◽

Gas Chromatography Mass Spectrometry ◽

Diffusion Method ◽

Citrus Species ◽

Citrus Peel ◽

Antioxidant And Antimicrobial Activities

Citrus species of plants are among the most commercially cultivated crops, mainly for their fruit. Besides, the generally consumed flesh inside the fruit, the peel is quite important too. Essential oils extracted from the peel have a history of being used by humankind for centuries. These essential oils are rich in antioxidants and antimicrobial agents. Comparative investigation of volatile constituents, and antioxidant and antimicrobial activities were undertaken. The essential oils were evaluated through gas chromatography-mass spectrometry (GC–MS), and enantiomeric composition by chiral GC–MS. Similarly, the antioxidant properties were evaluated by 2,2-diphenyl-1-picrylhydrazyl scavenging assay, and antimicrobial activities were assayed using the disk diffusion method. The highest extraction yield of 1.83% was observed in Citrus sinensis Osbeck. GC–MS analysis showed limonene (63.76–89.15%), γ-terpinene (0.24–6.43%), β-pinene (0.15–6.09%), linalool (0.35–3.5%), sabinene (0.77–2.17%), myrcene (0.74–1.75%), α-terpineol (0.28–1.15%), and α-pinene (0.2–0.58%) as the major constituents of the essential oil of the Citrus species studied. For the first time, through our study, chiral terpenoids have been observed from Citrus grandis Osbeck essential oil. The order of antioxidant activity is as follows: Citrus grandis Osbeck red flesh > Citrus reticulata Blanco > Citrus sinensis Osbeck > Citrus grandis Osbeck white flesh. Except for Citrus grandis Osbeck white flesh (52.34 µL/mL), all samples demonstrated stronger antioxidant activities than those of the positive control, quercetin (5.60 µL/mL). Therefore, these essential oils can be used as a safe natural antioxidant to prevent product oxidation. Likewise, citrus peel essential oil showed antimicrobial activity against tested bacterial strains, albeit marginal.

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Analysis of Volatile Flavor Compounds in Huizhou Preserved Vegetable by GC-MS

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.140.258 ◽

2011 ◽

Vol 140 ◽

pp. 258-262

Author(s):

Yu Ping Liu ◽

Zhi Wei Miao ◽

Jun Kai Yang ◽

Wei Guan ◽

Bao Guo Sun

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Dimethyl Disulfide ◽

Ethyl Benzoate ◽

Flavor Compounds ◽

Gas Chromatography Mass Spectrometry ◽

Volatile Flavor Compounds ◽

Volatile Flavor ◽

Simultaneous Distillation Extraction ◽

Aroma Characteristics

The volatile flavor compounds of Huizhou preserved vegetable were extracted by hydrodistillation (HD) and simultaneous distillation-extraction (SDE). The essential oils obtained were analyzed by gas chromatography-mass spectrometry, accompanying by comparison of the retention time of authentic samples and by calculation of retention index about the separated constituents. 50 volatile flavor compounds were identified in the essential oils of Huizhou preserved vegetable obtained by HD and SDE in all. 41 compounds were identified in the essential oil from HD and 36 compounds identified in the essential oil from SDE. The constituents which are very important to the odor of Huizhou preserved vegetable include 3-hydroxy-2-butanone, dimethyl disulfide, hexanal, furfural, 2-furanmethanol, 2-methylbutanoic acid, benzeneacetaldehyde, phenylethyl alcohol, 5-ethyldihydro-2(3H)-furanone, 5-pentyl-dihydro-2(3H)-furanone, etc. The exclusive compounds in the essential oil gained by HD consist of hexanol, heptanol, 1-(2-furanyl)-ethanone, 5-methyl-dihydro-2(3H)-furanone, benzaldehyde, phenol, benzyl alcohol, methyl benzoate, ethyl benzoate. The exclusive compounds obtained by SDE are 3-hydroxy-2-butanone, dimethyl trisulfide,trans-2-(2-pentenyl)-furan, camphor, vanillin, etc. The differences result in the two essential oils with a few distinctions on aroma characteristics.

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Volatilome Analyses and In Vitro Antimicrobial Activity of the Essential Oils from Five South African Helichrysum Species

Molecules ◽

10.3390/molecules25143196 ◽

2020 ◽

Vol 25 (14) ◽

pp. 3196 ◽

Cited By ~ 1

Author(s):

Basma Najar ◽

Valeria Nardi ◽

Claudio Cervelli ◽

Giulia Mecacci ◽

Francesca Mancianti ◽

...

Keyword(s):

Essential Oils ◽

South African ◽

Antibacterial Activities ◽

Antimicrobial Activities ◽

Gas Chromatography Mass Spectrometry ◽

Good Effect ◽

Similar Amount ◽

Microsporum Canis ◽

Agar Disc

Helichrysum genus was used in folk South African medicine to treat various human disorders. As a part of our on-going research addressing the exploitation of South African plants belonging to this genus, five species were investigated for their volatile and antimicrobial activities. The volatile organic compounds (VOCs) and the essential oils (EOs) were analysed by gas chromatography mass spectrometry (GC-MS). Microdilution was the method used for assessing both antimycotic and antibacterial activities, which was also tested by Kirby-Bauer agar disc diffusion. Total monoterpenes (TMs) dominated the VOCs of four species (H. trilineatum (70.6%), H. edwardsii (79.3%), H. cooperi (84.5%), and H. pandurifolium (57.0%)). H. cooperi and H. edwardsii EOs showed the predominance of TMs (68.2% and 84.5%, respectively), while H. pandurifolium and H. trilineatum EOs were characterized by the prevalence of TSs (86.5% and 43.6%, respectively). H. odoratissimum EO evidenced a similar amount of both TMs (49.5%) and TSs (46.4%). Microsporum canis was more sensitive to these EOs. The lowest minimum inhibitory concentration (MIC) was observed with H. pandurifolium and H. edwardsii EOs (0.25%). H. pandurifolium and H. trilineatum had a good effect on Staphylococcus aureus (MIC 5%). These findings open new perspectives for the exploitation of these natural compounds for application in cosmetics and pharmaceutics.

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Chemical Composition and Cytotoxic and Antibacterial Activities of the Essential Oil of Aloysia citriodora Palau Grown in Morocco

Advances in Pharmacological Sciences ◽

10.1155/2017/7801924 ◽

2017 ◽

Vol 2017 ◽

pp. 1-10 ◽

Cited By ~ 5

Author(s):

Moulay Ali Oukerrou ◽

Mounir Tilaoui ◽

Hassan Ait Mouse ◽

Inass Leouifoudi ◽

Abdeslam Jaafari ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Cytotoxic Activity ◽

Vero Cell ◽

Cell Lines ◽

Cytotoxic Effect ◽

Mass Spectrometry Analysis ◽

Gas Chromatography Mass Spectrometry ◽

Agar Disc ◽

Vero Cell Lines

The aim of this work is to investigate the in vitro cytotoxic and antibacterial effects of the essential oils of Aloysia citriodora Palau, harvested in different regions of Morocco. The chemical profile was established using gas chromatography-mass spectrometry analysis. The cytotoxic activity against P815, MCF7, and VERO cell lines as well as the normal human peripheral blood mononuclear cells (PBMCs) was evaluated using the MTT assay. Standard, ATCC, strains of bacteria (Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa) were cultivated in Muller Hinton media. Then, agar disc diffusion, minimum inhibitory concentrations (MICs), and minimal bactericidal concentrations (MBCs) were determined using microdilution method. The essential oils obtained were predominantly composed of β-spathulenol (15.61%), Ar-curcumene (14.15%), trans-caryophyllene oxide (14.14%), and neral (10.02%). The results of the assays showed that the cytotoxic effect of the essential oil of A. citriodora was high on P815 and moderate on MCF7 and on VERO cell lines. However, no cytotoxic effect was observed on PBMCs. On the other hand, essential oils showed a significant antimicrobial activity against both Gram-negative and Gram-positive bacteria. MICs ranged between 2.84 and 8.37 mg/ml. Essential oil of A. citriodora leaves possesses significant antibacterial effect and cytotoxic activity against tumor cell lines.

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COMPARATIVE STUDY OF METABOLITES AND ANTIMICROBIAL ACTIVITIES OF ESSENTIAL OILS EXTRACTED FROM THREE AMOMUM SUBULATUM CULTIVARS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i6.33215 ◽

2019 ◽

pp. 219-223

Author(s):

AFTAB ALAM ◽

RITA SINGH MAJUMDAR ◽

PRAVEJ ALAM

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Essential Oils ◽

Antimicrobial Activities ◽

Gas Chromatography Mass Spectrometry ◽

Dilution Method ◽

Minimal Bactericidal Concentration ◽

Agar Dilution Method ◽

Escherichia Coli Bacteria ◽

Amomum Subulatum

Objective: In Sikkim (India), Seremna is a highly growing cultivar of Amomum subulatum at lower altitudes. Other popular cultivated varieties are Varlangy and Sawney in the same state but at different altitude. In this study, we evaluate the variation in essential oils, metabolites and antimicrobial activities among A. subulatum selected cultivars. Methods: The composition of essential oil of Varlangy, Seremna, and Sawney was analyzed using gas chromatography–mass spectrometry and comparative antimicrobial activity of oils was explored using agar well diffusion and agar dilution method. Results: The Seremna cultivar oil was shown the high content of major constituents (1,8-cineole) in comparison to others. Comparative minimum inhibitory concentrations and minimal bactericidal concentration (MBC) or minimal fungicidal concentration against two Gram-positive (Staphylococcus aureus and Bacillus subtilis), two Gram-negative (Klebsiella pneumoniae and Escherichia coli) bacteria, and two (Candida albicans and Aspergillus niger) fungi were determined. The oil of Seremna showed distinct antibacterial and antifungal activity against all the microorganisms except B. subtilis which showed resistance. Conclusion: The present findings concluded that the high content of the principal compound accelerates the antimicrobial activity of essential oils. The essential oil of Seremna could be a good antimicrobial agent and recommended in the case of infections.

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Chemical Composition and Antimicrobial Activity of Essential Oil from Phytolacca dodecandra Collected in Ethiopia

Molecules ◽

10.3390/molecules24020342 ◽

2019 ◽

Vol 24 (2) ◽

pp. 342 ◽

Cited By ~ 3

Author(s):

Wondwosen Matebie ◽

Wanchang Zhang ◽

Guangbo Xie

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Solid Phase ◽

Antimicrobial Activities ◽

Gas Chromatography Mass Spectrometry ◽

Microdilution Method ◽

Phytolacca Dodecandra ◽

The Difference

The essential oil from Phytolacca dodecandra, a traditional herb of Ethiopia, has been studied, including the chemical composition and antimicrobial activity. The difference between four P. dodecandra samples (P-1–P-4), which differed in gender or location, has also been analyzed. The essential oils were obtained by steam distillation, while the aromas were extracted by head space solid-phase microextraction (HS-SPME) and both were analyzed by gas chromatography- mass spectrometry (GC-MS). The oils’ antimicrobial activities were evaluated by the microdilution method against Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Candida albicans. Ninety one components, representing 88.37 to 94.01% of the aromas, were identified. The compositions of the aromas of four samples are mainly dominated by aldehydes and ketones: 2-nonanone (1.80–30.80%), benzaldehyde (4.99–25.99%), and sulcatone (2.34–5.87%). Sixty components representing 64.61 to 69.64% of the oils were identified, and phytone (3.04–21.23%), phytol (4.11–26.29%) and palmitic acid (1.49–23.87%) are the major compounds. No obvious antimicrobial activity was observed for all the four essential oils.

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Chemical Analysis and Growth Inhibitory Effect Of Rosemary Plant on Aspergillus niger

Kurdistan Journal of Applied Research ◽

10.24017/science.2017.2.22 ◽

2017 ◽

Vol 2 (2) ◽

pp. 151-154

Author(s):

Tara Faeq M. Salih ◽

Lana MA. Mohammed ◽

Karzan Omer Qader

Keyword(s):

Antifungal Activity ◽

Aspergillus Niger ◽

Essential Oil ◽

Essential Oils ◽

Inhibitory Effect ◽

Gas Chromatography Mass Spectrometry ◽

Dilution Method ◽

Agar Dilution Method ◽

Main Compound ◽

Plant Extraction

The use of natural fungicides instead of synthetic ones covers is a present trend in mycological disease control. The quality and food safeties could be preserved by the addition of natural antifungal agents as essential oils. The aim of the present study was to assess the antifungal activity of certain essential oil against the genus of Aspergillus The oil of the aerial parts of Rosemary plant that collected from Sulaimani city was analyzed by using gas chromatography mass spectrometry. The antifungal activity of Rosemary plant extract against Aspergillus niger was assessed by using agar dilution method. The results showed identification of 17 compounds, with 80.62% of Benzene, 1-methoxy-4- (1-propenyl), 6.85% 1,3,6-Octatriene, 3,7-dimethyl-, (E), 5.16% 1,3,6-Octatriene, 3,7- dimethyl and 2.83% D-Limonene constitute the highest percentage of essential oil. The result of investigation the antifungal effect of Rosemary plant extraction dilutions showed that the essential oil has significant inhibitory effect on growth of Aspergillus niger in 10 %, 12%, 14%, 16%, 18%, 20%, 22%, 24%, 26%, and 28% dilutions with the average inhibitory growth percentage 25.91%, 25.60%, 28.96%, 28.96%, 30.18%, 32.31%, 28.04%, 28.65%, 31.40% and 31.70% respectively. Large percentage antifungal activities of Rosemary oil are related with Benzene, 1-methoxy-4-(1-propenyl) as the main compound. In conclusion, the essential oils exhibited a potential inhibition activity against toxic fungi and many invitro and invivo studies are requested to find more about the antifungal activity of these extracted oils.

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Phytochemical Study on the Essential Oils of Tarragon (Аrtemisia dracunculus L.) Growing in Tajikistan and Its Comparison With the Essential Oil of the Species in the Rest of the World

Natural Product Communications ◽

10.1177/1934578x20977394 ◽

2020 ◽

Vol 15 (12) ◽

pp. 1934578X2097739

Author(s):

Farukh S. Sharopov ◽

Aminjon Salimov ◽

Sodik Numonov ◽

Mahinur Bakri ◽

Zafar Sangov ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Hierarchical Cluster ◽

Antibacterial Activities ◽

Antimicrobial Activities ◽

Methyl Eugenol ◽

Gas Chromatography Mass Spectrometry ◽

Phytochemical Study ◽

Aerial Parts ◽

Total Oil

The aerial parts of the tarragon ( Artemisia dracunculus) were collected around Kukteppa village, Ziddi, Varzob region of Tajikistan. The essential oil of tarragon was obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry. Forty-five compounds representing 99.8% of total oil were identified. Sabinene (29.1%), estragole (24.6%), limonene (7.8%), ( Z)-artemidin (4.9%), myrcene (4.8%), and ( E)-β-ocimene (4.0%) were components with a representation higher than 4% of the essential oils of aerial parts of tarragon. Hierarchical cluster analysis of А. dracunculus essential oils on the global phytogeographic origin based on 30 essential oil components and 105 samples (globally) of this species, indicated the existence of its 7 major chemotypes: ocimene, α-terpinene, capillene, methyl eugenol, mixed chemotype, ( Z)-artemidin, and estragol chemotypes. The essential oils of А. dracunculus showed weak antioxidant and antibacterial activities. To our best knowledge, this is the first report concerning the chemical composition, chemotypic variation, antioxidant, and antimicrobial activities of the essential oils obtained from the aerial parts of А. dracunculus, growing wild in the Varzob region of Tajikistan.

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Antimicrobial Activity of Blended Essential Oil Preparation

Natural Product Communications ◽

10.1177/1934578x1200701041 ◽

2012 ◽

Vol 7 (10) ◽

pp. 1934578X1200701 ◽

Cited By ~ 7

Author(s):

Sarin Tadtong ◽

Supatcha Suppawat ◽

Anchalee Tintawee ◽

Phanida Saramas ◽

Suchada Jareonvong ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Essential Oils ◽

Volume Ratio ◽

Antimicrobial Effect ◽

Antimicrobial Activities ◽

Active Components ◽

Linalyl Acetate ◽

Lavender Oil ◽

Agar Disc

Antimicrobial activities of two blended essential oil preparations comprising lavender oil, petigrain oil, clary sage oil, ylang ylang oil and jasmine oil were evaluated against various pathogenic microorganisms. Both preparations showed antimicrobial activity in the agar disc diffusion assay against the Gram-positive bacteria, Staphylococcus aureus ATCC6538 and S. epidermidis isolated strain, the fungus, Candida albicans ATCC10231, and the Gram-negative bacterium, Escherichia coli ATCC25922, but showed no activity against Pseudomonas aeruginosa ATCC9027. The minimum inhibitory concentration (MIC) of these preparations was evaluated. By the broth microdilution assay, preparation 1, comprising lavender oil, clary sage oil, and ylang ylang oil (volume ratio 3:4:3), exhibited stronger antimicrobial activity than preparation 2, which was composed of petigrain oil, clary sage oil, and jasmine oil (volume ratio 3:4:3). Moreover, the sum of the fractional inhibitory concentrations (Σfic) of preparation 1 expressed a synergistic antimicrobial effect against the tested microorganisms (Σfic<1). The blended essential oil preparations, characterized for their components by GC/MS, contained linalyl acetate, and linalool as major components. Our experiments showed that the differential antimicrobial effect of either blended oil preparations or single/pure essential oils may be influenced by the amount of linalool and linalyl acetate, and the number of active components in either the blended preparations or single/pure essential oils. In addition, blended oil preparations expressed synergistic antimicrobial effect by the accumulation of active components such as linalool and linalyl acetate and combining active constituents of more than one oil.

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