scholarly journals Comparative Assessment of Phytochemical Profiles of Comfrey (Symphytum officinale L.) Root Extracts Obtained by Different Extraction Techniques

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 837 ◽  
Author(s):  
Nataša Nastić ◽  
Isabel Borrás-Linares ◽  
Jesús Lozano-Sánchez ◽  
Jaroslava Švarc-Gajić ◽  
Antonio Segura-Carretero
Keyword(s):  
Fatty Acids ◽  
High Performance ◽  
Polar Compounds ◽  
Extraction Techniques ◽  
Root Extracts ◽  
Flight Mass Spectrometry ◽  
Solvent Type ◽  
Chemical Profiles ◽  
Solid Liquid ◽  
Phytochemical Profiles

In this work a comparative study on phytochemical profiles of comfrey root extracts obtained by different extraction approaches has been carried out. Chemical profiles of extracts obtained by supercritical fluid (SFE), pressurized liquid (PLE), and conventional solid/liquid extraction were compared and discussed. Phytochemical composition was assessed by high-performance liquid chromatography coupled with electrospray time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS/MS) identifying 39 compounds reported for the first time in comfrey root, mainly phenolic acids and fatty acids. The influence of different extraction parameters on phytochemical profiles of S. officinale root was investigated for all applied techniques. PLE and maceration, using alcohol-based solvents (aqueous methanol or ethanol), were shown to be more efficient in the recovery of more polar compounds. Greater numbers of phenolics were best extracted by PLE using 85% EtOH at 63 °C. The use of SFE and 100% acetone for 30 min enabled good recoveries of nonpolar compounds. SFE using 15% EtOH as a cosolvent at 150 bar produced the best recoveries of a significant number of fatty acids. The main compositional differences between extracts obtained by different extraction techniques were assigned to the solvent type. Hence, these results provided comprehensive approaches for treating comfrey root enriched in different phytochemicals, thereby enhancing its bioaccessibility.

scholarly journals Sweet Cherry Byproducts Processed by Green Extraction Techniques as a Source of Bioactive Compounds with Antiaging Properties

Antioxidants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 418 ◽  
Author(s):  
Luz Agulló-Chazarra ◽  
Isabel Borrás-Linares ◽  
Jesús Lozano-Sánchez ◽  
Antonio Segura-Carretero ◽  
Vicente Micol ◽  
...  
Keyword(s):  
Supercritical Co2 ◽  
High Performance ◽  
Food Industry ◽  
Sweet Cherry ◽  
Subcritical Water ◽  
Extraction Techniques ◽  
Green Extraction ◽  
Flight Mass Spectrometry ◽  
Cosmetic Industry ◽  
Continuous Demand

In the cosmetic industry, there is a continuous demand for new and innovative ingredients for product development. In the context of continual renovation, both cosmetic companies and customers are particularly interested in compounds derived from natural sources due to their multiple benefits. In this study, novel and green-extractive techniques (pressurized solvent, supercritical CO2, and subcritical water extractions) were used to obtain three new extracts from sweet cherry stems, a byproduct generated by the food industry. The extracts were characterized by high-performance liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS), and 57 compounds, mainly flavonoids but also organic and phenolic acids, fatty acids, and terpenes, were identified. After analytical characterization, a multistep screening approach, including antioxidant, enzymatic, and photoprotective cellular studies, was used to select the best extract according to its benefits of interest to the cosmetics industry. The extract obtained with supercritical CO2 presented the best characteristics, including a wide antioxidant capacity, especially against lipid peroxyl and •OH free radicals, as well as relevant photoprotective action and antiaging properties, making it a potential new ingredient for consideration in the development of new cosmetics.

scholarly journals Lipid Compositions and Geographical Discrimination of 94 Geographically Authentic Wheat Samples Based on UPLC-MS with Non-Targeted Lipidomic Approach

Foods ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 10
Author(s):  
Mengchu Jin ◽  
Wenhao Zheng ◽  
Yaqiong Zhang ◽  
Boyan Gao ◽  
Liangli (Lucy) Yu
Keyword(s):  
Fatty Acids ◽  
High Performance ◽  
Unsaturated Fatty Acids ◽  
Principal Component ◽  
Geographical Differences ◽  
Safety Control ◽  
Flight Mass Spectrometry ◽  
Geographical Discrimination ◽  
Lipid Compounds ◽  
Chemical Characters

Wheat is the staple food for the world’s major populations. However, chemical characters of geographically authentic wheat samples, especially for the lipids, have not been deeply studied. The present research aimed to investigate lipid compositions of Chinese wheat samples and clarify the major markers that contribute to the geographical differences. A total of 94 wheat samples from eight main wheat-producing provinces in China were evaluated to differentiate their lipid compositions. Based on the data collected from ultra-high-performance-liquid-chromatography tandem time-of-flight mass spectrometry (UPLC-Q/TOF MS), an optimized non-targeted lipidomic method was utilized for analyses. As the results, 62 lipid compounds, including fatty acids, phospholipids, galactolipids, triglycerides, diglycerides, alkylresorcinol, and ceramide were tentatively identified. Partial least squares discriminant analysis (PLS-DA) demonstrated a more satisfying performance in distinguishing wheat samples from different origins compared with principal component analysis (PCA). Further, the abundances of triglycerides and glycerophospholipids with more unsaturated fatty acids were found greater in wheat samples from northern origins of China, while more glycolipids and unsaturated fatty acids arose in southern original wheat samples. These findings describe the lipid profiles of wheat samples in China and could contribute to the quality and safety control for the wheat flour products.

scholarly journals Monitoring the Bioactive Compounds Status in Olea europaea According to Collecting Period and Drying Conditions

Energies ◽  
2019 ◽  
Vol 12 (5) ◽  
pp. 947 ◽  
Author(s):  
Amani Taamalli ◽  
Jesus Lozano Sánchez ◽  
Haifa Jebabli ◽  
Najla Trabelsi ◽  
Leila Abaza ◽  
...  
Keyword(s):  
High Performance ◽  
Reversed Phase ◽  
Olive Leaf ◽  
Leaf Extracts ◽  
Ionization Time ◽  
Drying Temperature ◽  
Flight Mass Spectrometry ◽  
Health Promoting ◽  
Drying Conditions ◽  
Phytochemical Profiles

Polyphenols and triterpenoids in olive have relevant importance both in the physiology of the plant and the nutritional and biological value of its products. Olive leaf extracts are of special interest for their numerous health-promoting properties. The present research is investigating the occurrence of phytochemicals in supercritical fluid extracts from leaves with regard to collection time and drying temperature. The phytochemical profiles of the olive leaf extracts were determined by reversed-phase high-performance liquid chromatography (HPLC) coupled to electrospray ionization time-of-flight mass spectrometry (ESI-TOF-MS) detection. The main extracted phytochemicals were phenols and terpenoids. A significant variation in the amounts of the different components was observed as a function of the different drying temperature and collecting time (p < 0.05). Among samples, the maximal contents of polyphenols and secoiridoid derivatives were found in the extracts from olive leaves collected in November and dried at 120 °C, whereas triterpenoids showed the highest content in fresh leaves collected in August.

scholarly journals Lamiophlomis rotata Identification via ITS2 Barcode and Quality Evaluation by UPLC-QTOF-MS Couple with Multivariate Analyses

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3289 ◽  
Author(s):  
Wang Jian ◽  
Gao YunLing ◽  
Chen YiLong ◽  
Chen YiWen ◽  
Zhang Yi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance ◽  
Multivariate Analyses ◽  
Dna Barcodes ◽  
Multivariate Statistical ◽  
Flight Mass Spectrometry ◽  
Chemical Profiles ◽  
Nj Tree ◽  
First Time

Lamiophlomis rotata (L. rotata), is known as “Daba” in the Tibetan region, Ajuga ovalifolia and Oreosolen wartii have also been utilized as substitutes for “Daba”, however, only L. rotata has been officially listed in the Chinese Pharmacopoeia for hemostasis preparations. To safely apply the traditional uses of the herb, internal transcribed spacer 2 (ITS2) DNA barcodes were employed to discriminate L. rotata from its adulterants. For further evaluation of the quality of different originating habitats, the chemical profiles of 25 samples were determined by ultra-high-performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC-QTOF-MS) coupled with multivariate analyses. ITS2 DNA barcodes differentiated L. rotata from O. wartii and A. ovalifolia accurately. A neighbor-joining (NJ) tree showed that three origins clustered into three clades. Forty-nine compounds were identified in the total ion current (TIC) profile of L. rotata. Additionally, two pairs of isomers were identified for the first time by using mass spectrometry fragmentation. The differences between the variable habitats were determined by multivariate statistical analysis of the UPLC-QTOF-MS data from 25 specimens. Ten compounds were identified as the characteristic markers distinguishing the sample from four geographical origins. The results also suggest that samples from Qinghai and Sichuan province would be the most suitable choice for traditional prescriptions and preparations.

UHPLC-ESI-QTOF-MS Analysis on Canthium Coromandelicum Stem

2021 ◽  
Vol 25 (11) ◽  
pp. 41-46
Author(s):  
Arockia Raj A. Anto ◽  
J. Vinnarasi
Keyword(s):  
Mass Spectrometry ◽  
Amino Acids ◽  
Fatty Acids ◽  
Quality Control ◽  
High Performance ◽  
Triple Quadrupole ◽  
Flight Mass Spectrometry ◽  
Anthraquinone Glycosides ◽  
Proof Verification ◽  
Fragmentation Patterns

A rapid ultra high-performance liquid chromatography coupled with crossover triple quadrupole time of flight mass spectrometry (UHPLC- ESI -QTOF-MS/MS) method has been developed for the identification of debasement products. According to the distinctive fragmentation patterns, the presence of 79 compounds with retention time between 1.05 to 26.81 minutes was found. In Canthium coromandelicum stem, 22 amino acids, 10 fatty acids, 6 alkaloids, 6 steroids, 2 flavonoids, 2 terpenoids, 2 phenolic, 4 lipids, 3 anthraquinone glycosides, sugars, vitamins were distinguished. These outcomes demonstrated that the contemporary technique has been utilized for quality control of Canthium coromandelicum, exceptionally for recognizable proof, verification and portrayal in medication arrangements.

scholarly journals Metabolic Profile of Scytalidium parasiticum-Ganoderma boninense Co-Cultures Revealed the Alkaloids, Flavonoids and Fatty Acids that Contribute to Anti-Ganoderma Activity

Molecules ◽  
2020 ◽  
Vol 25 (24) ◽  
pp. 5965
Author(s):  
Rafidah Ahmad ◽  
Choon Kiat Lim ◽  
Nurul Fadhilah Marzuki ◽  
Yit-Kheng Goh ◽  
Kamalrul Azlan Azizan ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
High Performance ◽  
Culture Media ◽  
Biological Control Agent ◽  
Control Agent ◽  
Ganoderma Boninense ◽  
Flight Mass Spectrometry ◽  
Metabolomics Study ◽  
Antimicrobial Metabolites

In solving the issue of basal stem rot diseases caused by Ganoderma, an investigation of Scytalidium parasiticum as a biological control agent that suppresses Ganoderma infection has gained our interest, as it is more environmentally friendly. Recently, the fungal co-cultivation has emerged as a promising method to discover novel antimicrobial metabolites. In this study, an established technique of co-culturing Scytalidium parasiticum and Ganoderma boninense was applied to produce and induce metabolites that have antifungal activity against G. boninense. The crude extract from the co-culture media was applied to a High Performance Liquid Chromatography (HPLC) preparative column to isolate the bioactive compounds, which were tested against G. boninense. The fractions that showed inhibition against G. boninense were sent for a Liquid Chromatography-Time of Flight-Mass Spectrometry (LC-TOF-MS) analysis to further identify the compounds that were responsible for the microbicidal activity. Interestingly, we found that eudistomin I, naringenin 7-O-beta-D-glucoside and penipanoid A, which were present in different abundances in all the active fractions, except in the control, could be the antimicrobial metabolites. In addition, the abundance of fatty acids, such as oleic acid and stearamide in the active fraction, also enhanced the antimicrobial activity. This comprehensive metabolomics study could be used as the basis for isolating biocontrol compounds to be applied in oil palm fields to combat a Ganoderma infection.

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