Synthesis, Complexation Properties, and Evaluation of New Aminodiphosphonic Acids as Vector Molecules for 68Ga Radiopharmaceuticals

Two new aminodiphosphonic acids derived from salicylic acid and its phosphonic analogue were prepared through a simple and efficient synthesis. 2-[(2-Amino-2,2-diphosphono)ethyloxy]-benzoic acid 8 and 2-[(2-amino-2,2-diphosphono)ethyloxy]-5-ethyl-phenylphosphonic acid 9 were evaluated for their applicability as 68Ga binding bone-seeking agents. Protonation constants of 8 and 9 and stability constants of the Ga3+ complexes with 8 and 9 in water were determined. The stability constant of Ga3+ complex with fully phosphorylated acid 9 (logKGaL = 31.92 ± 0.32) significantly exceeds stability constant of Ga3+ complex with 8 (logKGaL = 26.63 ± 0.24). Ligands 8 and 9 are as effective for Ga3+ cation binding as ethylenediamine-N,N’-diacetic-N,N’-bis(methy1enephosphonic) acid and ethylenediamine-N,N,N’,N’-tetrakis(methylenephosphonic) acid, respectively. The labelling process and stability of [68Ga]Ga-8 and [68Ga]Ga-9 were studied. Both 8 and 9 readily form 68Ga-complexes stable to ten-fold dilution with saline. However, in fetal bovine serum, only [68Ga]Ga-9 was stable enough to be subject to biological evaluation. It was injected into rats with bone pathology and aseptic inflammation of soft tissues. For [68Ga]Ga-9 in animals with a bone pathology model in 60 and 120 min after injection, a slight accumulation in the pathology site, stable blood percentage level, and moderate accumulation in the liver were observed. For animals with an aseptic inflammation, the accumulation of [68Ga]Ga-9 in the pathology site was higher than that in animals with bone pathology. Moreover, the accumulation of [68Ga]Ga-9 in inflammation sites was more stable than that for [68Ga]Ga-citrate. The percentage of [68Ga]Ga-9 in the blood decreased from 3.1% ID/g (60 min) to 1.5% ID/g (120 min). Accumulation in the liver was comparable to that obtained for [68Ga]Ga-citrate.

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Improving the Stability of Short Hairpin RNA against Fetal Bovine Serum Using the Third Double-Stranded RNA-Binding Domain from Staufen Protein

Biological and Pharmaceutical Bulletin ◽

10.1248/bpb.32.283 ◽

2009 ◽

Vol 32 (2) ◽

pp. 283-288 ◽

Cited By ~ 2

Author(s):

Yasuhisa Kawaguchi ◽

Kazumi Danjo ◽

Tomoyuki Okuda ◽

Hirokazu Okamoto

Keyword(s):

Bovine Serum ◽

Rna Binding ◽

Fetal Bovine Serum ◽

Binding Domain ◽

Hairpin Rna ◽

Double Stranded Rna ◽

The Third ◽

Fetal Bovine ◽

Short Hairpin ◽

The Stability

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Resveratrol Can Be Stable in a Medium Containing Fetal Bovine Serum with Pyruvate but Shortens the Lifespan of Human Fibroblastic Hs68 Cells

Oxidative Medicine and Cellular Longevity ◽

10.1155/2018/2371734 ◽

2018 ◽

Vol 2018 ◽

pp. 1-15 ◽

Cited By ~ 2

Author(s):

Yuan-Jhe Chang ◽

Ya-Chun Chang ◽

Rosa Huang Liu ◽

Chia-Wen Chen ◽

Inn Lee ◽

...

Keyword(s):

Bovine Serum ◽

Fetal Bovine Serum ◽

Oxidative Degradation ◽

Sod Activity ◽

Replicative Lifespan ◽

Fetal Bovine ◽

The Stability

This study is aimed at developing a method that can inhibit resveratrol (Res) degradation in Dulbecco’s modified Eagle medium (DMEM) and at evaluating the effects of Res on the replicative lifespan of Hs68 cells. We hypothesized that Res can extend the lifespan of Hs68 cells if we can inhibit the oxidative degradation of Res in the medium. We found that the addition of ≥5 U/mL SOD to the medium could completely inhibit Res degradation in DMEM. Fetal bovine serum (FBS) contained 29.3 ± 1.1 U/mL of SOD activity. FBS could prevent Res degradation in the medium through SOD activity and Res–FBS interaction, but the regular FBS concentration (i.e., 10% FBS) exhibited an insufficient ability to completely inhibit Res degradation. We found that pyruvate (1 mM) could potentiate SOD to scavenge superoxide at approximately 2.2-fold. Thus, 10% FBS combined with pyruvate (1 mM) could completely inhibit Res degradation. When Res was not degraded, it still shortened the lifespan of Hs68 cells. Overall, the proposed method involving 10% FBS combined with pyruvate (1 mM) could completely prevent Res degradation. However, in contrast to our hypothesis, Res could induce the shortening of the lifespan of Hs68 cells. The stability of Res analogs (i.e., oxy-Res and acetyl-Res) in the medium and their effects on the lifespan of Hs68 cells were also investigated.

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Fetal bovine serum influences the stability and bioactivity of resveratrol analogues: A polyphenol-protein interaction approach

Food Chemistry ◽

10.1016/j.foodchem.2016.09.154 ◽

2017 ◽

Vol 219 ◽

pp. 321-328 ◽

Cited By ~ 34

Author(s):

Fen Tang ◽

Yixi Xie ◽

Hui Cao ◽

Hua Yang ◽

Xiaoqing Chen ◽

...

Keyword(s):

Protein Interaction ◽

Bovine Serum ◽

Fetal Bovine Serum ◽

Fetal Bovine ◽

Resveratrol Analogues ◽

The Stability ◽

Interaction Approach

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Involvement of nucleus in the maturation of dengue-2 virus in C6/36 cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100162120 ◽

1990 ◽

Vol 48 (3) ◽

pp. 912-913

Author(s):

Jaang J. Wang ◽

Cheng C. Chen ◽

Men F. Shaio ◽

Chia T. Liu ◽

Chung S. Lee ◽

...

Keyword(s):

Culture Medium ◽

Cytopathic Effect ◽

Fetal Bovine Serum ◽

Virus Particles ◽

Electron Microscopies ◽

Embedding Technique ◽

Fetal Bovine ◽

Flat Embedding ◽

20 Nm ◽

Labeling Method

The involvement of nucleus in the maturation processes of Dengue-2 virus in a mosquito cell line, C6/36 cells, has been identified by the electron microscopy and immunocytochemistry. The C6/36 cells were obtained from ATCC and maintained in MEM culture medium containing 10% fetal bovine serum at 28°C. The cell suspensions or cells grown on teflon-coated coverslips were infected with Dengue-2 virus (107/ml) for various time periods of 2 hours, 3, 6, 8, and 10 days. The cells were then fixed in buffered 1.5% glutaraldehyde, and washed in acetone before immunolabeled with monoclonal antibody. An indirect immunocytochemical labeling method of avidin-biotin complex (ABC) conjugated with peroxidase or gold particles (20 nm in diameter) and a flat embedding technique were used to localize the virus particles.At early stages of infections (before 3 days), there were no virion particles detected. After 6 days and on of infections, cytopathic effect (CPE) was observed and showed positive immuno-peroxidase reactions under the light and electron microscopies.

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Treatment of fetal bovine serum improves early development of porcine embryos by alleviating oxidative stress

Reproduction Abstracts ◽

10.1530/repabs.1.p070 ◽

2014 ◽

Author(s):

Seon-A Choi ◽

Seong-Eun Mun ◽

Pil-Soo Jeong ◽

Hae-Jun Yang ◽

Seung-Bin Yoon ◽

...

Keyword(s):

Oxidative Stress ◽

Early Development ◽

Bovine Serum ◽

Fetal Bovine Serum ◽

Fetal Bovine

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МОДЕЛИРОВАНИЕ СИСТЕМ ЭКСТРАКОРПОРАЛЬНОГО СОЗРЕВАНИЯ ООЦИТОВ КРУПНОГО РОГАТОГО СКОТА С ИСПОЛЬЗОВАНИЕМ СТРУКТУРНЫХ КОМПОНЕНТОВ ОВАРИАЛЬНЫХ ФОЛЛИКУЛОВ *

Molochnoe i miasnoe skotovodstvo ◽

10.33943/mms.2019.6.39671 ◽

2019 ◽

pp. 20-22

Author(s):

T.I. KUZMINA ◽

I.V. CHISTYAKOVA

Keyword(s):

Granulosa Cells ◽

Follicular Fluid ◽

Bovine Serum ◽

Egg Quality ◽

Fetal Bovine Serum ◽

Reproductive Technologies ◽

Blastocyst Stage ◽

Preimplantation Embryos ◽

Cultural Systems ◽

Fetal Bovine

Создание эффективной унифицированной системы дозревания донорских ооцитов обеспечит повышение результативности инновационных клеточных репродуктивных технологий. В исследовании проведен сравнительный мониторинг показателеймейотического созревания ооцитов коров, созревших в различных системах, дополненных структурными компонентами фолликулов (СКФ стенки фолликулов, клетки гранулезы, белки) и фолликулярной жидкостью,а также потенций к развитию из них доимплантационных эмбрионов. Анализу подверглись ооциты, прокультивированные в следующих системах:среда ТС199 с добавлением 10 фетальной бычьей сыворотки (ФБС), 50 мкг/мл эстрадиола, 10 мкг/мл лютеинизирующего гормона (ЛГ), 10 мкг/мл фолликулостимулирующего гормона (ФСГ) среда ТС199 с 10 эстральной сывороткой коров среда ТС199 с 50 жидкости из фолликулов диаметром 9 мм среда ТС199 с добавлением белков фолликулярной жидкости молекулярной массой 65 кДасреда ТС199 с 10 ФБС и 1106 клеток гранулезы среда ТС199 с 10 ФБС и тканью фолликула. В культуральные среды ко всем исследованным группам ооцитов добавляли антибиотики. Использование CКФ обеспечило значительное снижение доли ооцитов с дегенерированным хроматином, что способствовало увеличению уровня доимпланационных эмбрионов на стадии бластоцисты. Так, доля бластоцист, развившихся из ооцитов, созревших в среде со стенками фолликулов,составила43,5. В этой же группе выявлен минимальный уровень дегенерированных зародышей (6,45). Полученные данные предлагается использовать при моделировании систем дозревания ооцитов коров с целью повышения качества яйцеклеток.The creation of an effective unified maturation system of donor oocytes provides an increase in the efficiency of innovative cellular reproductive technologies. The comparative analysis of the meiotic maturation indicators of bovine oocytes, which were matured in different cultural systems modified by follicular structural components (FSC follicular walls, granulosa cells, proteins) and follicular fluid, as well as the potential for preimplantation embryonic development were evaluated in this study. Oocytes matured in following cultural systems: medium TC199 supplemented with 10 fetal bovine serum and 50 g/ml of estradiol, 10 g/ml of luteinizing hormone (LH), 10 g/ml of folliclestimulating hormone (FSH) medium TC199 with 10 estrous cow serum medium TC199 with 50 liquid from follicles with a diameter of 9 mm medium TC199 supplemented with the follicular fluid proteins with molecular weight 65 kDa medium TC199 with 10 fetal bovine serum and 1106 granulosa cells medium TC199 with the addition of 10 fetal bovine serum and follicle tissues were analyzed. Antibiotics were added to cultural media of all experimental groups of oocytes. The usage of FSC ensured the decrease in the proportion of oocytes with degenerated chromatin, which contribute the rise of the level of preimplantation embryos at the blastocyst stage. Thus, the proportion of blastocysts developed from oocytes matured in medium supplemented with follicular walls was 43.5. In the same experimental group, the number of degenerated embryos was 6.45. The obtained data are supposed to be used for modeling the cultural systems of cow oocytes in order to improve the egg quality.

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Synthesis and solvatochromic studies of 2- amino-3-phenolazo1-(4-sulfophenyl)-3-methyi-5-pyrozolone and use it for the determination of trace amount of Nickel (II) in blood samples

International Journal of Bioassays ◽

10.21746/ijbio.2016.10.001 ◽

2016 ◽

Vol 5 (10) ◽

pp. 4920

Author(s):

Amar M. Ali ◽

Hussain. J. Mohammed*

Keyword(s):

Stability Constant ◽

Trace Amount ◽

Benzenesulfonic Acid ◽

Determination Method ◽

Blood Samples ◽

Determination Of Nickel ◽

Normal Human ◽

The Stability ◽

Normal Human Blood

A new, simple, sensitive and rapid spectrophotometric method is proposed for the determination of trace amount of Nickel (II). The method is based on the formation of a 1:2 complex with 4-(4-((2-hydroxy-6-nitrophenyl) diazenyl) -3-methyl-5-oxo-2, 5-dihydro-1H-pyrazol-1-yl) benzenesulfonic acid (2-ANASP) as a new reagent is developed. The complex has a maximum absorption at 516 nm and εmax of 1. 84 X 105 L. mol-1. cm-1. A linear correlation (0. 25 – 4. 0μg. ml-1) was found between absorbance at λmax and concentration. The accuracy and reproducibility of the determination method for various known amounts of Nickel (II) were tested. The results obtained are both precise (RSD was 1. 2 %) and accurate (relative error was 0. 787 %). The effect of diverse ions on the determination of Nickel (II) to investigate the selectivity of the method were also studied. The stability constant of the product was 0. 399 X 106 L. mol-1. The proposed method was successfully applied to the analysis of diabetes blood and normal human blood.

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