scholarly journals Sustainable Surfactin Production by Bacillus subtilis Using Crude Glycerol from Different Wastes

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3488
Author(s):  
Tomasz Janek ◽  
Eduardo J. Gudiña ◽  
Xymena Połomska ◽  
Piotr Biniarz ◽  
Dominika Jama ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Crude Glycerol ◽  
Culture Media ◽  
Low Cost ◽  
Carbon Sources ◽  
Chemical Characterization ◽  
Raw Material ◽  
Waste Glycerol ◽  
Wide Range ◽  
Surfactin Production

Most biosurfactants are obtained using costly culture media and purification processes, which limits their wider industrial use. Sustainability of their production processes can be achieved, in part, by using cheap substrates found among agricultural and food wastes or byproducts. In the present study, crude glycerol, a raw material obtained from several industrial processes, was evaluated as a potential low-cost carbon source to reduce the costs of surfactin production by Bacillus subtilis #309. The culture medium containing soap-derived waste glycerol led to the best surfactin production, reaching about 2.8 g/L. To the best of our knowledge, this is the first report describing surfactin production by B. subtilis using stearin and soap wastes as carbon sources. A complete chemical characterization of surfactin analogs produced from the different waste glycerol samples was performed by liquid chromatography–mass spectrometry (LC-MS) and Fourier transform infrared spectroscopy (FTIR). Furthermore, the surfactin produced in the study exhibited good stability in a wide range of pH, salinity and temperatures, suggesting its potential for several applications in biotechnology.

scholarly journals Burkholderia thailandensis E264 as a promising safe rhamnolipids’ producer towards a sustainable valorization of grape marcs and olive mill pomace

2021 ◽  
Author(s):  
Alif Chebbi ◽  
Massimiliano Tazzari ◽  
Cristiana Rizzi ◽  
Franco Hernan Gomez Tovar ◽  
Sara Villa ◽  
...  
Keyword(s):  
Energy Source ◽  
Olive Oil ◽  
Low Cost ◽  
Carbon Sources ◽  
Burkholderia Thailandensis ◽  
Rhamnolipid Production ◽  
Key Points ◽  
Wide Range ◽  
Long Chain ◽  
Olive Mill

Abstract Within the circular economy framework, our study aims to assess the rhamnolipid production from winery and olive oil residues as low-cost carbon sources by nonpathogenic strains. After evaluating various agricultural residues from those two sectors, Burkholderia thailandensis E264 was found to use the raw soluble fraction of nonfermented (white) grape marcs (NF), as the sole carbon and energy source, and simultaneously, reducing the surface tension to around 35 mN/m. Interestingly, this strain showed a rhamnolipid production up to 1070 mg/L (13.37 mg/g of NF), with a higher purity, on those grape marcs, predominately Rha-Rha C14-C14, in MSM medium. On olive oil residues, the rhamnolipid yield of using olive mill pomace (OMP) at 2% (w/v) was around 300 mg/L (15 mg/g of OMP) with a similar CMC of 500 mg/L. To the best of our knowledge, our study indicated for the first time that a nonpathogenic bacterium is able to produce long-chain rhamnolipids in MSM medium supplemented with winery residues, as sole carbon and energy source. Key points • Winery and olive oil residues are used for producing long-chain rhamnolipids (RLs). • Both higher RL yields and purity were obtained on nonfermented grape marcs as substrates. • Long-chain RLs revealed stabilities over a wide range of pH, temperatures, and salinities

scholarly journals Utilization of Food Processing By-products in Extrusion Processing: A Review

2021 ◽  
Vol 4 ◽  
Author(s):  
Debomitra Dey ◽  
Jana K. Richter ◽  
Pichmony Ek ◽  
Bon-Jae Gu ◽  
Girish M. Ganjyal
Keyword(s):  
Functional Properties ◽  
Low Cost ◽  
Value Added ◽  
Raw Material ◽  
Practical Applications ◽  
Extrusion Processing ◽  
High Production Rate ◽  
Wide Range ◽  
Extruded Products ◽  
By Products

The processing of agricultural products into value-added food products yields numerous by-products or waste streams such as pomace (fruit and vegetable processing), hull/bran (grain milling), meal/cake (oil extraction), bagasse (sugar processing), brewer's spent grain (brewing), cottonseed meal (cotton processing), among others. In the past, significant work in exploring the possibility of the utilization of these by-products has been performed. Most by-products are highly nutritious and can be excellent low-cost sources of dietary fiber, proteins, and bioactive compounds such as polyphenols, antioxidants, and vitamins. The amount of energy utilized for the disposal of these materials is far less than the energy required for the purification of these materials for valorization. Thus, in many cases, these materials go to waste or landfill. Studies have been conducted to incorporate the by-products into different foods in order to promote their utilization and tackle their environmental impacts. Extrusion processing can be an excellent avenue for the utilization of these by-products in foods. Extrusion is a widely used thermo-mechanical process due to its versatility, flexibility, high production rate, low cost, and energy efficiency. Extruded products such as direct-expanded products, breakfast cereals, and pasta have been developed by researchers using agricultural by-products. The different by-products have a wide range of characteristics in terms of chemical composition and functional properties, affecting the final products in extrusion processing. For the practical applications of these by-products in extrusion, it is crucial to understand their impacts on the qualities of raw material blends and extruded products. This review summarizes the general differences in the properties of food by-products from different sources (proximate compositions, physicochemical properties, and functional properties) and how these properties and the extrusion processing conditions influence the product characteristics. The discussion of the by-product properties and their impacts on the extrudates and their nutritional profile can be useful for food manufacturers and researchers to expand their applications. The gaps in the literature have been highlighted for further research and better utilization of by-products with extrusion processing.

scholarly journals Exploiting the Sequence of Naturally Occurring Elastin: Construction, Production and Characterization of a Recombinant Thermoplastic Protein-Based Polymer

2009 ◽  
Vol 6 ◽  
pp. 133-145 ◽  
Author(s):  
Raul Machado ◽  
A.J. Ribeiro ◽  
J. Padrão ◽  
D. Silva ◽  
A. Nobre ◽  
...  
Keyword(s):  
Culture Media ◽  
Low Cost ◽  
Carbon Sources ◽  
Molecular Weight Polymer ◽  
Precise Control ◽  
Self Assembling ◽  
Thermoplastic Protein ◽  
Optimization Of Fermentation ◽  
Absolute Control

Genetic engineering was used to produce an elastin-like polymer (ELP) with precise amino acid composition, sequence and length, resulting in the absolute control of MW and stereochemistry. A synthetic monomer DNA sequence encoding for (VPAVG)20, was used to build a library of concatemer genes with precise control on sequence and size. The higher molecular weight polymer with 220 repeats of VPAVG was biologically produced in Escherichia coli and purified by hot and cold centrifugation cycles, based on the reversible inverse temperature transition property of ELPs. The use of low cost carbon sources like lactose and glycerol for bacteria cells culture media was explored using Central Composite Design approach allowing optimization of fermentation conditions. Due to its self-assembling behaviour near 33 °C stable spherical microparticles with a size ~ 1µm were obtained, redissolving when a strong undercooling is achieved. The polymer produced showed hysteresis behaviour with thermal absorbing/releasing components depending on the salt concentration of the polymer solution.

scholarly journals Production of Poly(3-hydroxybutyrate) (P(3HB)) from Different Agroindustry Byproducts by Bacillus megaterium

2021 ◽  
Vol 11 (6) ◽  
pp. 14278-14289
Keyword(s):  
Organic Carbon ◽  
Bacillus Megaterium ◽  
Culture Media ◽  
Carbon Sources ◽  
Modern Society ◽  
Cell Biomass ◽  
Wide Range ◽  
Pha Production ◽  
Factorial Design Of Experiments ◽  
Dry Cell

The use of plastics, replacing materials such as paper and glass, has become a staple of modern society. A possible alternative is the production of biopolymers, in which case polyhydroxyalkanoates (PHAs) tend to be the most studied class. Among the PHAs, poly(3-hydroxybutyrate) (P(3HB)) is one of the most studied. Bacillus megaterium can utilize a wide range of carbon sources to produce P(3HB) and display high resistance to osmotic pressure and temperature. There are few studies regarding the viability of confectionery wastewater (CWW) and rice parboiling water (RPW) in PHA production; however, data suggests they might be successfully used as culture media for microorganisms. Cultures of B. megaterium were conducted under a 22-factorial design of experiments with different agitation and airflow conditions using mineral medium added with CWW and RPW. Under maximized conditions, it was possible to obtain 7.55 g/L of dry cell biomass, with 50.1% P(3HB) content (3.78 g/L). Kinetic parameters of the maximized culture were also assessed, with the highest productivity (0.146 g/L·h) being found after 20 h of culture. Results found in the tests indicate that CWW and RPW can be used as culture media in P(3HB) production; however further studies are necessary to fully optimize the process, as well as to find alternatives to reduce the amount of residual total organic carbon (TOC) still present in the cultures.

scholarly journals A Biorefinery Approach to the Biomass of the Seaweed Undaria pinnatifida (Harvey Suringar, 1873): Obtaining Phlorotannins-Enriched Extracts for Wound Healing

Biomolecules ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 461
Author(s):  
Carolina A. M. Ferreira ◽  
Rafael Félix ◽  
Carina Félix ◽  
Adriana P. Januário ◽  
Nuno Alves ◽  
...  
Keyword(s):  
Wound Healing ◽  
Undaria Pinnatifida ◽  
Low Cost ◽  
Chemical Characterization ◽  
Liquid Extraction ◽  
Antioxidant Power ◽  
Wide Range ◽  
Ferric Reducing Antioxidant Power ◽  
Anti Inflammatory ◽  
Antioxidant And Antimicrobial Activity

Brown seaweeds are recognized sources of compounds with a wide range of properties and applications. Within these compounds, phlorotannins are known to possess several bioactivities (e.g., antioxidant, anti-inflammatory, and antimicrobial) with potential to improve wound healing. To obtain phlorotannins enriched extracts from Undaria pinnatifida, a biorefinery was set using low-cost industry-friendly methodologies, such as sequential solid–liquid extraction and liquid–liquid extraction. The obtained extracts were screened for their antioxidant and antimicrobial activity against five common wound pathogens and for their anti-inflammatory potential. The ethanolic wash fraction (wE100) had the highest antioxidant activity (114.61 ± 10.04 mmol·mg−1 extract by Diphenyl-1-picrylhydrazyl (DPPH) and 6.56 ± 1.13 mM eq. Fe II·mg−1 extract by and Ferric Reducing Antioxidant Power (FRAP)), acting efficiently against Gram-negative (Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus) bacteria, and showing a nitric oxide production inhibition over 47% when used at 0.01 µg·mL−1. NMR and FTIR chemical characterization suggested that phlorotannins are present. Obtained fraction wE100 proved to be a promising candidate for further inclusion as wound healing agents, while the remaining fractions analyzed are potential sources for other biotechnological applications, giving emphasis to a biorefinery and circular economy framework to add value to this seaweed and the industry.

scholarly journals Evaluation of adsorptive desulfurization performance and economic applicability comparison of activated carbons prepared from various carbon sources

RSC Advances ◽  
2020 ◽  
Vol 10 (66) ◽  
pp. 40329-40340
Author(s):  
Kun Chen ◽  
Weining Li ◽  
Bernard Wiafe Biney ◽  
Zhuo Li ◽  
Jiahua Shen ◽  
...  
Keyword(s):  
Preparation Method ◽  
Activated Carbons ◽  
Low Cost ◽  
Carbon Sources ◽  
Environmentally Friendly ◽  
Raw Material ◽  
Adsorptive Desulfurization

Selection and preparation method of a low-cost, environmentally-friendly desulfurization adsorbent raw material.

scholarly journals Fermented rice bran: an alternative ingredient in baking

2020 ◽  
Vol 9 (11) ◽  
pp. e45491110225 ◽  
Author(s):  
Anelise Christ-Ribeiro ◽  
Janaína Barreto Alves ◽  
Leonor Almeida de Souza-Soares ◽  
Eliana Badiale-Furlong
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Rice Bran ◽  
Scientific Community ◽  
Animal Feed ◽  
Culture Media ◽  
Low Cost ◽  
Raw Material ◽  
By Products

The idea that associated agro-industrial by-products only with the production of animal feed has been gradually being replaced, as the scientific community is interested in these products, as low-cost nutrients for the composition of food and/or culture media in research biotechnological to increase or improve nutrients or characteristics inherent in the raw material. Thus, this work aims to characterize input for baking with claim of natural fortification from the fermentation by Saccharomyces cerevisiae. For this, biomass was characterized in terms of its composition (ashes, fibers, lipids, proteins, and carbohydrates) and antioxidant activity of phenolic compounds. The results show an increase in nutrients such as ash, proteins and fibers, in addition the free and bound phenolic compounds present in the biomass showed antioxidant potential. Therefore, fermented rice bran is a potential input to be used in baking.

scholarly journals Glycerol or crude glycerol as substrates make Pseudomonas aeruginosa achieve anaerobic production of rhamnolipids

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Feng Zhao ◽  
Yuting Wu ◽  
Qingzhi Wang ◽  
Mengyao Zheng ◽  
Qingfeng Cui
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Crude Glycerol ◽  
Low Cost ◽  
Carbon Sources ◽  
Anaerobic Growth ◽  
Strain Specificity ◽  
Spectra Analysis ◽  
Anoxic Environments ◽  
Anaerobic Production

Abstract Background The anaerobic production of rhamnolipids is significant in research and application, such as foamless fermentation and in situ production of rhamnolipids in the anoxic environments. Although a few studies reported that some rare Pseudomonas aeruginosa strains can produce rhamnolipids anaerobically, the decisive factors for anaerobic production of rhamnolipids were unknown. Results Two possible hypotheses on the decisive factors for anaerobic production of rhamnolipids by P. aeruginosa were proposed, the strains specificity of rare P. aeruginosa (hypothesis 1) and the effect of specific substrates (hypothesis 2). This study assessed the anaerobic growth and rhamnolipids synthesis of three P. aeruginosa strains using different substrates. P. aeruginosa strains anaerobically grew well using all the tested substrates, but glycerol was the only carbon source that supported anaerobic production of rhamnolipids. Other carbon sources with different concentrations still failed for anaerobic production of rhamnolipids by P. aeruginosa. Nitrate was the excellent nitrogen source for anaerobic production of rhamnolipids. FTIR spectra analysis confirmed the anaerobically produced rhamnolipids by P. aeruginosa using glycerol. The anaerobically produced rhamnolipids decreased air-water surface tension to below 29.0 mN/m and emulsified crude oil with EI24 above 65%. Crude glycerol and 1, 2-propylene glycol also supported the anaerobic production of rhamnolipids by all P. aeruginosa strains. Prospects and bottlenecks to anaerobic production of rhamnolipids were also discussed. Conclusions Glycerol substrate was the decisive factor for anaerobic production of rhamnolipids by P. aeruginosa. Strain specificity resulted in the different anaerobic yield of rhamnolipids. Crude glycerol was one low cost substrate for anaerobic biosynthesis of rhamnolipids by P. aeruginosa. Results help advance the research on anaerobic production of rhamnolipids, deepen the biosynthesis theory of rhamnolipids and optimize the anaerobic production of rhamnolipids.

scholarly journals High value-added products derived from crude glycerol via microbial fermentation using Yarrowia clade yeast

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Magdalena Rakicka-Pustułka ◽  
Joanna Miedzianka ◽  
Dominika Jama ◽  
Sylwia Kawalec ◽  
Kamila Liman ◽  
...  
Keyword(s):  
Lipid Content ◽  
Food Production ◽  
Crude Glycerol ◽  
Animal Feed ◽  
Carbon Sources ◽  
Value Added ◽  
Microbial Fermentation ◽  
Waste Glycerol ◽  
High Consumption ◽  
Value Added Products

Abstract Background Contemporary biotechnology focuses on many problems related to the functioning of developed societies. Many of these problems are related to health, especially with the rapidly rising numbers of people suffering from civilization diseases, such as obesity or diabetes. One factor contributing to the development of these diseases is the high consumption of sucrose. A very promising substitute for this sugar has emerged: the polyhydroxy alcohols, characterized by low caloric value and sufficient sweetness to replace table sugar in food production. Results In the current study, yeast belonging to the Yarrowia clade were tested for erythritol, mannitol and arabitol production using crude glycerol from the biodiesel and soap industries as carbon sources. Out of the 13 tested species, Yarrowia divulgata and Candida oslonensis turned out to be particularly efficient polyol producers. Both species produced large amounts of these compounds from both soap-derived glycerol (59.8–62.7 g dm−3) and biodiesel-derived glycerol (76.8–79.5 g dm−3). However, it is equally important that the protein and lipid content of the biomass (around 30% protein and 12% lipid) obtained after the processes is high enough to use this yeast in the production of animal feed. Conclusions The use of waste glycerol for the production of polyols as well as utilization of the biomass obtained after the process for the production of feed are part of the development of modern waste-free technologies.

scholarly journals The Role of Heterotrophic Microalgae in Waste Conversion to Biofuels and Bioproducts

Processes ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 1090
Author(s):  
Teresa Lopes da Silva ◽  
Patrícia Moniz ◽  
Carla Silva ◽  
Alberto Reis
Keyword(s):  
Unsaturated Fatty Acids ◽  
Waste Treatment ◽  
Culture Media ◽  
Low Cost ◽  
Algal Growth ◽  
Value Added ◽  
Production Costs ◽  
Microalgal Biomass ◽  
Heterotrophic Microalgae ◽  
Wide Range

In the last few decades, microalgae have attracted attention from the scientific community worldwide, being considered a promising feedstock for renewable energy production, as well as for a wide range of high value-added products such as pigments and poly-unsaturated fatty acids for pharmaceutical, nutraceutical, food, and cosmetic markets. Despite the investments in microalgae biotechnology to date, the major obstacle to its wide commercialization is the high cost of microalgal biomass production and expensive product extraction steps. One way to reduce the microalgae production costs is the use of low-cost feedstock for microalgae production. Some wastes contain organic and inorganic components that may serve as nutrients for algal growth, decreasing the culture media cost and, thus, the overall process costs. Most of the research studies on microalgae waste treatment use autotrophic and mixotrophic microalgae growth. Research on heterotrophic microalgae to treat wastes is still scarce, although this cultivation mode shows several benefits over the others, such as higher organic carbon load tolerance, intracellular products production, and stability in production all year round, regardless of the location and climate. In this review article, the use of heterotrophic microalgae to simultaneously treat wastes and produce high value-added bioproducts and biofuels will be discussed, critically analyzing the most recent research done in this area so far and envisioning the use of this approach to a commercial scale in the near future.

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