scholarly journals Evaluation of Antioxidative Mechanisms In Vitro and Triterpenes Composition of Extracts from Silver Birch (Betula pendula Roth) and Black Birch (Betula obscura Kotula) Barks by FT-IR and HPLC-PDA

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4633
Author(s):  
Aleksandra Ostapiuk ◽  
Łukasz Kurach ◽  
Maciej Strzemski ◽  
Jacek Kurzepa ◽  
Anna Hordyjewska

Silver birch, Betula pendula Roth, is one of the most common trees in Europe. Due to its content of many biologically active substances, it has long been used in medicine and cosmetics, unlike the rare black birch, Betula obscura Kotula. The aim of the study was therefore to compare the antioxidant properties of extracts from the inner and outer bark layers of both birch trees towards the L929 line treated with acetaldehyde. Based on the lactate dehydrogenase test and the MTT test, 10 and 25% concentrations of extracts were selected for the antioxidant evaluation. All extracts at tested concentrations reduced the production of hydrogen peroxide, superoxide anion radical, and 25% extract decreased malonic aldehyde formation in acetaldehyde-treated cells. The chemical composition of bark extracts was accessed by IR and HPLC-PDA methods and surprisingly, revealed a high content of betulin and lupeol in the inner bark extract of B. obscura. Furthermore, IR analysis revealed differences in the chemical composition of the outer bark between black and silver birch extracts, indicating that black birch may be a valuable source of numerous biologically active substances. Further experiments are required to evaluate their potential against neuroinflammation, cancer, viral infections, as well as their usefulness in cosmetology.

Author(s):  
A. K. Kamysbaevа ◽  
◽  
G. E. Azimbaeva ◽  

Currently, 40% of drugs used in modern medicine are obtained from plant raw materials. Among the wild flora there are medicinal plant species containing a variety of chemical composition, including biologically active substances with antimicrobial activity and phytoncidal properties. This article presents biologically active substances of some species of the genus Asteraceae . The chemical composition of plants belonging to the Asteraceae family is represented by anthocyanins, inulins, pectins, flavonoids, proteins, cloves, fatty oils, coumarins, carotenes and polyphenols. The plant of the Asteraceae family is used in folk medicine as an anti-inflammatory, antitumor and cardiotonic agent.


2021 ◽  
Vol 51 (2) ◽  
pp. 301-311
Author(s):  
Bayana Bazhenova ◽  
Anastasia Burkhanova ◽  
Yuliya Zabalueva ◽  
Roman Dobretsky

Introduction. Antioxidant system is one of the main cellular mechanisms of adaptation. It is able to neutralize the destructive effect of free radicals, both external and internal. Functional products, especially of meat origin, can provide necessary antioxidant properties. Some types of plant raw materials possess a high content of polyphenol complexes with antioxidant properties. Therefore, introduction of phytonutrients into the composition of meat products can help to expand the range of foods with an antioxidant effect. Daurian rosehip is rich in biologically active substances and can increase their safety during processing. The research objective was to study the possibility of immobilization of Daurian rosehip antioxidants by protein-lipid inclusion. Study objects and methods. The research featured fruits of Daurian rosehip, its aqueous alcoholic extract, and protein-lipid complex. Results and discussion. The article presents experimental data on the chemical composition of Daurian rosehip. The optimal method with the best antioxidant results was a microwave phytoextraction by an aqueous alcoholic solution at a power of 800 W for 6–7 min. The antioxidants were immobilized by including the protein-lipid complex into the gel. The experiment demonstrated excellent functional and technological characteristics of the resulting system. Conclusion. The research revealed the optimal modes of microwave extraction of biologically active substances of Daurian rosehip. The rosehip extract was included into the protein-lipid complex and immobilized antioxidants, thus increasing their efficiency.


Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1330
Author(s):  
Stanislav Sukhikh ◽  
Svetlana Noskova ◽  
Svetlana Ivanova ◽  
Liubov Skrypnik ◽  
Artem Pungin ◽  
...  

The medicinal plant Dactylorhiza maculata (L.) Soó (family Orchidaceae) is used to treat gastritis, colic, gastrointestinal tract, and bladder diseases. This study aimed to investigate the properties and characteristics of the in vitro Dactylorhiza maculata extract. The recommended parameters for producing Dactylorhiza maculata extract were determined: temperature 60 °C, process duration 60 min, hydro module 1:10. It is recommended to carry out the extraction using an aqueous ethyl alcohol solution with a mass fraction of the parent substance of 70%. It was found that such biologically active substances as rutin, quercetin, 3,3’,4’,5,5’,7-hexahydroxyflavonone, 3,3’,4’,5,5’,7-hexahydroxyflavonone-3-O-glycoside, gallic acid, and ferulic acid were dominant in Dactylorhiza maculata. A high phosphorus content was noted (2410.8 mg/kg dry matter). The studied Dactylorhiza maculata samples contained a large number of organic acids and water-soluble vitamins. The tested extracts were safe in terms of the content of heavy metals, pesticides, aflatoxin B1, and radionuclides, as well as pathogenic and opportunistic microorganisms; the content was significantly lower than the threshold limit values. The studied complex of biologically active substances from Dactylorhiza maculata extract samples had antimicrobial properties. It was found that the antioxidant activity of the samples was 217.89 ± 10.89 mg AA/g (AA—ascorbic acid). The high content of bioactive substances and the antimicrobial and antioxidant properties of Dactylorhiza maculata extract samples determine the application potential of this plant as a substitute for growth stimulants and feed antibiotics in the production of feed additives, aiming to increase the physiological and immune status of livestock and poultry.


Author(s):  
Gennady Ivanovich Kasyanov ◽  
Arthur Magomedovich Magomedov ◽  
Svetlana Vasilievna Zolotokopova

The article presents the results of technological and merchandising research on the development of formulations of frozen semi-finished products from sweet pepper stuffed with minced fish, bulgur, quinoa and couscous, enriched by CO2-extracts with spices and smoking preparation. Feasibility of making frozen semi-finished products from fish is confirmed by an increase in consumer demand for these products, besides, shock freezing of semi-finished products with liquid nitrogen helps to preserve the most valuable components of the original animal and plant raw materials. The technology of cooking stuffed pepper from zoned raw materials is presented: sweet pepper sorts “Pride of Russia”, “Yellow Bull”, “Topolyn” grown in the open ground of the environmentally friendly Novopokrovsky district of the Krasnodar region by experts of the company “Vegan Line”, and muscle tissue of grass carp migrated from the Far East, acclimatized in the water bodies of Kuban and the Astrakhan region. For the first time in technological practice there the wheat grain modified products - bulgur and couscous with biologically active substances have been added to minced meat. A detailed analysis of chemical composition of pepper sorts mixture and grass carp caught in the Anapa district of the Krasnodar region was performed. In the course of studying the chemical composition of bulgur, quinoa and couscous there was stated a high content of alible proteins, vitamins, macro- and microelements. There have been formulated three recipes of stuffed sweet pepper of yellow, green and red colors: with bulgur, couscous and quinoa. A hardware-technological scheme has been developed for producing semi-finished sweet pepper stuffed with minced fish, cereals and CO2-extracts of spices and smoke preparation. There has been analyzed the chemical composition of the finished semi-finished products, provides information about the nutritional and biological value of the product, indicating a higher content of basic food and biologically active substances.


Author(s):  
Roksolana Konechna ◽  
Оksana Khropot ◽  
Romana Petrina ◽  
Maria Kurka ◽  
Zoriana Gubriy ◽  
...  

Objective: This study was undertaken to research of antioxidant properties of extracts of the plants and the callus biomass.Methods: The implementation of the method of cultivating the cells, tissues, and organisms in vitro for the reception of biological material of Carlina аcaulis L. (Asteraceae) is a timely issue for the medical and pharmaceutic industry, as the domestication in vitro creates the opportunity for the year-round production of the plant material as the source of biologically active substances. The seeds and plant materials (roots) of C. acaulis L. collected from natural habitat - meadows mountains Klyva Tarnychkovain Ukraine in the Carpathians. The influence of phytohormones on growth processes of plant cells is researched; the optimal conditions for the cultivation of C. acaulis L. are defined and chosen. Biomass extract is obtained and researched for the presence of biologically active substances and conducted definition of oxygen radical absorbance capacity (ORAC) of extracts of medical herbs and callus biomass of C. acaulis L.Results: C. аcaulis L. was cultivated in vitro. There was set up a scheme of sterilization of seeds with the biggest outcome of aseptic explants. There took place the investigation of the qualitative content of the extracts of callus biomass of C. acaulis L. and the detection of flavonoids and tannins. Conducted definition of ORAC of extracts of medical herbs and callus biomass of C. acaulis L. Conclusion: The obtained data showed that the investigated extracts of callus biomass and biomass of the medical herb C. acaulis L. having antioxidant activity, which makes it possible to continue research in this direction.


2021 ◽  
pp. 23-27
Author(s):  
Сергей Валерьевич Штерман ◽  
Сергей Александрович Иванов ◽  
Михаил Юрьевич Сидоренко ◽  
Валерий Соломонович Штерман ◽  
Юрий Ильич Сидоренко

Основная задача, возлагаемая на спортивные энергетические напитки - это снабжение атлетов дополнительным импульсом внутренней энергии. В составе разработанного спортивного напитка «Inka Cola» в качестве источника биологически активных веществ использовали экстракт семян южноамериканского кустарника - гуараны. Специфическая черта этого растения заключается в высокой концентрации кофеина в его семенах, содержание которого примерно в 4-5 раз выше, чем в зернах кофе. Наличие в составе напитка «Inka Cola» компонентов, содержащихся в экстракте семян гуараны, позволяет снизить усталость атлетов и обеспечить длительное поддержание высокого уровня их физической и когнитивной активности. В качестве другого источника биологически активных веществ был включен экстракт травы готу-колы, которая произрастает в странах Юго-Восточной Азии. Компоненты, входящие в состав этого продукта, позволяют бороться с эмоциональными расстройствами, способствуют улучшению памяти и восстановлению нервных клеток в мозге человека, а также обладают широким спектром противовоспалительных, иммуностимулирующих, антибактериальных и антиоксидантных свойств. С целью повышения энергетической ценности и улучшения вкусового восприятия напитка «Inka сola» в его состав была включена изомальтулоза - изомер сахарозы с пониженным значением гликемического индекса; для повышения физиологического воздействия на организм спортсменов биогенные минеральные компоненты - калий и магний; для создания гармоничного вкусового восприятия напитка - регулятор кислотности (лимонная кислота) и привлекательных реологических свойств напитка - загуститель (ксантановая камедь). С целью формирования цвето-ароматического образа напитка «Inka cola» в его состав был включен ароматизатор «мохито» и конгруэнтный, то есть соответствующий ему по своему сенсорному восприятию, пищевой краситель. По отзывам спортсменов, использовавших напиток «Inka cola» в течение нескольких недель, у них снижалась утомляемость, им легче становилось воспринимать и усваивать информацию, а также отмечалось усиление физической и умственной выносливости. The main goal of sports energy drinks is to provide athletes with an additional impulse of internal energy. In the composition of the developed sports drink «Inka Cola» as a source of biologically active substances was used an extract of the seeds of the South American shrub - guarana. A specific feature of this plant is the high concentration of caffeine in its seeds, the content of which is about 4-5 times higher than in coffee beans. The inclusion of guarana seed extract in the «Inka Cola» drink composition helps to reduce fatigue of athletes and ensure long-term maintenance of a high level of their physical and cognitive activity. An extract of the gotu-kola herb, which grows in the countries of Southeast Asia, was used as another source of biologically active substances. The constituents of this extract allow to fight emotional disorders, improve memory and restore nerve cells in the human brain. They also have a wide range of anti-inflammatory, immunostimulating, antibacterial and antioxidant properties. In order to increase the energy value and improve the taste perception of the «Inka Cola» drink, isomaltulose, an isomer of sucrose with a reduced glycemic index, was included in its composition; to increase the physiological impact on the athletes were used important biogenic mineral components - potassium and magnesium; to create a harmonious taste perception of the drink - acidity regulator (citric acid) and attractive rheological properties of the drink - thickener (xanthan gum) were included. The formation of the color-aromatic image of the drink «Inka cola» was carried out by using in its composition of the flavoring product «mojito» and congruent food coloring. According to the reviews of athletes who used the «Inka Cola» drink for several weeks, their fatigue decreased, it became easier for them to perceive and assimilate information, as well as an increase in physical and mental endurance was noted.


Cellulose ◽  
2019 ◽  
Vol 26 (5) ◽  
pp. 3047-3067 ◽  
Author(s):  
Hubert Lachowicz ◽  
Hanna Wróblewska ◽  
Magdalena Sajdak ◽  
Magdalena Komorowicz ◽  
Rafał Wojtan

2020 ◽  
Vol 50 (4) ◽  
pp. 707-716
Author(s):  
Valery Bogdanov ◽  
Andrei Simdiankin ◽  
Anna Pankina ◽  
Vadim Mostovoi

Introduction. Nutrition has a significant impact on human health, performance, well-being, and lifespan. The research objective was to develop formulations for functional compositions of seafood cryoconcentrates. Study objects and methods. The study featured concentrates obtained by cryotechnology from carcasses of Pacific squid (Todarodes pacificus), muscular shell of cucumaria (Cucumaria japonica), scallop mantle (Mizuhopecten yessoensis), octopus skin (Octopus dofleini), milt of Pacific herring (Pacific herring), and Japanese kelp (Laminaria japonica), as well as their multicomponent mixes. The content of biologically active substances was determined by the spectrophotometric method. The safety and biological value were biotested on Tetrahymena pyriformis. Results and discussion. The dry seafood concentrates had a high content of protein, minerals, and biologically active components, i.e. hexosamines, triterpene glycosides, carotenoids, nucleic acids, etc. The seafood cryoconcentrate mixes were divided into fortifying, restoring, immunomodulating, and mineral. Their chemical composition varied greatly, depending on the functional aim of the mix. For instance, the protein content varied from 28.8 to 57.6%, lipids – from 3.1 to 6.9%, carbohydrates – from 15.0 to 35.9%, minerals – from 10.9 to 22.9%, and hexosamines – from 2.2 to 5.6%. The experiments made it possible to substantiate the composition of the dry mixes. The compilation principle was based on the chemical profile of the raw material, as well as on the choice of biologically active substances. All the dry seafood concentrates showed a high relative biological value. However, the values differed, depending on the type of raw material: from 65.5% for the scallop mantle sample to 86.3% for the octopus skin sample. The difference could be explained by the chemical composition of the cryopowders and their availability to ciliate enzymes. Conclusion. The research resulted in formulations and functional mixes of dry seafood concentrates obtained by cryotechnology. The dry seafood concentrates can be used both independently and as functional compositions. The developed mixes demonstrated rather high content of animal proteins, minerals, and biologically active substance, e.g. hexosamines, triterpene glycosides, carotenoids, nucleic acids, etc. Their functional compositions proved to have excellent sensory properties; they are biologically safe for human consumption and have a high relative biological value.


Author(s):  
V. V. Makukha ◽  
Т. I. Bokova

 The main groups of biologically active substances contained in the bark of Betula pendula were determined by the spectrophotometric method: triterpene saponins, coumarins, hydroxycinnamic acids, tannins. It was found that the place of growth affects the content of triterpene saponins. There is an increase in their content from the anthropogenic load. A comparative analysis of the quantitative content of coumarins in samples of birch bark showed the similarity of indicators, so we can conclude that the place of growth does not significantly affect the content of coumarins. Studies have shown that all the samples under study accumulate approximately the same amount (about 1.5 %) of hydroxycinnamic acids. The difference between the content of the studied substances is small, but it exists. Studies of the content of tannins found that the place of growth of the hanging birch does not significantly affect this indicator. But with the increase in anthropogenic impact, we can note an increase in the content of this group of BAS. The study of the content of amino acids showed that the area of growth of the hanging birch affects their content in the bark. Anthropogenic factors also affect the content of amino acids, with an increase in the anthropogenic load on the growing area, the content of amino acids becomes higher.


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