scholarly journals Application of Three Ecological Assessment Tools in Examining Chromatographic Methods for the Green Analysis of a Mixture of Dopamine, Serotonin, Glutamate and GABA: A Comparative Study

Molecules ◽  
2021 ◽  
Vol 26 (18) ◽  
pp. 5436
Author(s):  
Atiah H. Almalki ◽  
Ibrahim A. Naguib ◽  
Fahad S. Alshehri ◽  
Badrah S. Alghamdi ◽  
Hashem O. Alsaab ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Assessment Tools ◽  
Gamma Aminobutyric Acid ◽  
Analysis Method ◽  
Fast Analysis ◽  
Gaba A ◽  
Qualitative And Quantitative ◽  
Chromatographic Methods ◽  
Scope Of Application ◽  
National Environmental

The assessment of greenness of analytical protocols is of great importance now to preserve the environment. Some studies have analyzed either only the neurotransmitters, dopamine, serotonin, glutamate, and gamma-aminobutyric acid (GABA), together or with other neurotransmitters and biomarkers. However, these methods have not been investigated for their greenness and were not compared with each other to find the optimum one. Therefore, this study aims to compare seven published chromatographic methods that analyzed the four neurotransmitters and their mixtures using the National Environmental Method Index, Analytical Eco-Scale Assessment (ESA), and Green Analytical Procedure Index (GAPI). As these methods cover both qualitative and quantitative aspects, they offer better transparency. Overall, GAPI showed maximum greenness throughout the analysis. Method 6 was proven to be the method of choice for analyzing the mixture, owing to its greenness, according to NEMI, ESA, and GAPI. Additionally, method 6 has a wide scope of application (13 components can be analyzed), high sensitivity (low LOQ values), and fast analysis (low retention times, especially for glutamate and GABA).

Facile and Fast Detection of Genistein in Derris scandens by Square Wave Voltammetry using a Cobalt(II) Phthalocyanine-Modified Screen-Printed Electrochemical Sensor

2020 ◽  
Vol 16 (3) ◽  
pp. 341-348
Author(s):  
Surinya Traipop ◽  
Suchada Chuanuwatanakul ◽  
Orawon Chailapakul ◽  
Eakkasit Punrat
Keyword(s):  
Electrochemical Sensor ◽  
Square Wave Voltammetry ◽  
Silver Chloride ◽  
Reference Electrode ◽  
High Sensitivity ◽  
Plastic Substrate ◽  
Fast Analysis ◽  
Square Wave ◽  
Wave Voltammetry ◽  
Screen Printed

Background: Recently, Derris scandens, a Thai herbal medicine with anti-inflammatory activity, is widely used as beverage and supplementary food. When the traditional medicine is a choice for health therapy, the simple and reliable equipment is required to control the suitable consuming amount of the active component. Objective: To develop the electrochemical sensor for genistein determination in Derris scandens with high sensitivity and rapid operation. Methods: An in-house screen-printed electrochemical sensor consisting of a three-electrode system was developed for genistein determination. A silver/silver chloride (Ag/AgCl) reference electrode, a carbon counter electrode and a carbon working electrode were prepared on a 0.3-mm-thick plastic substrate by the screen-printing technique using conductive ink. The dimensions of each sensor were 2.5×1.0 cm. Only 50 µL of sample solution was required on this device for the determination of genistein concentration by rapid response square wave voltammetry. Results: The oxidation peak of genistein appeared with good response in acidic media at a peak potential of 0.6 V. Moreover, the signal was enhanced by modifying the conductive carbon ink with cobalt( II) phthalocyanine. Under the optimized conditions, the linear range was found to be 2.5-150 µM and the detection limit was 1.5 µM. Moreover, the small volume extraction was successfully developed without any further pre-concentration. This proposed method was applied to determine genistein in Derris scandens with satisfying results. Conclusion: The proposed method is promising as an alternative method for genistein determination with facile and fast analysis.

Gamma-aminobutyric acid (GABA): a para- and/or autocrine hormone in the pituitary

2001 ◽  
Vol 15 (6) ◽  
pp. 1089-1091 ◽  
Author(s):  
ARTUR MAYERHOFER ◽  
BARBARA HÖHNE-ZELL ◽  
KATIA GAMEL-DIDELON ◽  
HEIKE JUNG ◽  
PETER REDECKER ◽  
...  
Keyword(s):  
Aminobutyric Acid ◽  
Gamma Aminobutyric Acid ◽  
Gaba A

scholarly journals Alternative fast analysis method for cellulose sponge surface sampling wipes with low concentrations of Bacillus Spores

2019 ◽  
Vol 156 ◽  
pp. 5-8 ◽  
Author(s):  
Ahmed Abdel-Hady ◽  
M. Worth Calfee ◽  
Denise Aslett ◽  
Sang Don Lee ◽  
Barbara Wyrzykowska-Ceradini ◽  
...  
Keyword(s):  
Analysis Method ◽  
Surface Sampling ◽  
Fast Analysis ◽  
Cellulose Sponge ◽  
Low Concentrations ◽  
Bacillus Spores

scholarly journals The Russian Aphasia Test: The first comprehensive, quantitative, standardized, and computerized aphasia language battery in Russian

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0258946
Author(s):  
Maria V. Ivanova ◽  
Yulia S. Akinina ◽  
Olga A. Soloukhina ◽  
Ekaterina V. Iskra ◽  
Olga V. Buivolova ◽  
...  
Keyword(s):  
Psychometric Properties ◽  
Characteristic Curve ◽  
High Sensitivity ◽  
Clinical Work ◽  
Assessment Tools ◽  
Assessment Instrument ◽  
Auditory Comprehension ◽  
Specific Level ◽  
Cutoff Values ◽  
Wide Range

The lack of standardized language assessment tools in Russian impedes clinical work, evidence-based practice, and research in Russian-speaking clinical populations. To address this gap in assessment of neurogenic language disorders, we developed and standardized a new comprehensive assessment instrument–the Russian Aphasia Test (RAT). The principal novelty of the RAT is that each subtest corresponds to a specific level of linguistic processing (phonological, lexical-semantic, syntactic, and discourse) in different domains: auditory comprehension, repetition, and oral production. In designing the test, we took into consideration various (psycho)linguistic factors known to influence language performance, as well as specific properties of Russian. The current paper describes the development of the RAT and reports its psychometric properties. A tablet-based version of the RAT was administered to 85 patients with different types and severity of aphasia and to 106 age-matched neurologically healthy controls. We established cutoff values for each subtest indicating deficit in a given task and cutoff values for aphasia based on the Receiver Operating Characteristic curve analysis of the composite score. The RAT showed very high sensitivity (> .93) and specificity (> .96), substantiating its validity for determining presence of aphasia. The test’s high construct validity was evidenced by strong correlations between subtests measuring similar linguistic processes. The concurrent validity of the test was also strong as demonstrated by a high correlation with an existing aphasia battery. Overall high internal, inter-rater, and test-retest reliability were obtained. The RAT is the first comprehensive aphasia language battery in Russian with properly established psychometric properties. It is sensitive to a wide range of language deficits in aphasia and can reliably characterize individual profiles of language impairments. Notably, the RAT is the first comprehensive aphasia test in any language to be fully automatized for administration on a tablet, maximizing further standardization of presentation and scoring procedures.

scholarly journals The Prehospital Ambulance Stroke Test vs. the Cincinnati Prehospital Stroke Scale: a diagnostic accuracy study

2020 ◽  
Vol 17 ◽  
Author(s):  
Somayeh Karimi ◽  
Hassan Motamed ◽  
Ehsan Aliniagerdroudbari ◽  
Sepideh Babaniamansour ◽  
Arman Jami ◽  
...  
Keyword(s):  
Diagnostic Accuracy ◽  
Facial Palsy ◽  
High Sensitivity ◽  
Assessment Tools ◽  
Screening Tools ◽  
Eye Position ◽  
Diagnostic Accuracy Study ◽  
Specificity And Sensitivity ◽  
Accuracy Study ◽  
Chain Of Care

IntroductionImmediate diagnosis of stroke is crucial in reducing its morbidity and mortality rate. There are various pre-hospital assessment tools, such as the Prehospital Ambulance Stroke Test (PreHAST) and the Cincinnati Prehospital Stroke Scale (CPSS) used to identify stroke early in the chain of care. The aim of this study is to compare the accuracy of PreHAST with CPSS in diagnosing stroke.MethodsIn this diagnostic accuracy study patients with suspicion of stroke were included in this study. In CPSS, the criterion used to indicate stroke are facial droop, speech and arm drift. In PreHAST it is eye position, visual field, facial palsy, right and left arms paresis, right and left legs paresis, sensory and speech. After data collection, sensitivity and specificity were calculated using standard formulae. Different cut-off points for the best diagnostic accuracy were examined in both CPSS and PreHAST.ResultsIn this study, 883 patients were investigated. The results demonstrated that in CPSS, the highest specificity and sensitivity was for facial droop (84.9%) and arm drift (82.7%); and in PreHAST it was eye position (99.6%) and facial palsy (49.2%). The best predictor of stroke in CPSS with highest sensitivity (78.5%) and specificity (66%) was a cut-off point of 1.5 (AUC: 0.744) (p<0.01(. In PreHAST, the highest sensitivity (68.4%) was a cut-off point of 2.5 and the highest specificity (90.2%) was a cut-off point of 5.5 (AUC: 0.775) (p<0.01).ConclusionBoth PreHAST and CPSS are useful screening tools in the pre-hospital diagnosis of stroke. In addition to high sensitivity, these tests provide a grading system in which higher cut-off points lead to higher specificity.

Sensor system using polarization analysis method to monitor oil-on-water in water purification plants and rivers

2002 ◽  
Vol 45 (4-5) ◽  
pp. 167-174
Author(s):  
Y. Sanuki ◽  
M. Hiraoka ◽  
T. Ohto ◽  
H. Kanai ◽  
H. Tada
Keyword(s):  
Water Purification ◽  
Oil Spills ◽  
High Sensitivity ◽  
Visual Observation ◽  
Sensor System ◽  
Polarization Analysis ◽  
Analysis Method ◽  
Foreign Matter ◽  
Influence Of Water ◽  
Continuous Sensor

Some 80% of accidental pollution in river water is caused by oil spills. Oil spills can cause serious damage such as suspension of water intake at water purification plants and major harm to ecosystems in the lower reaches of rivers. This is because oil-on-water tends to spread easily, quickly exacerbating the damage. To address this problem, an automated, continuous sensor system with high sensitivity can be used for early detection of spill accidents. We have developed a sensor system for detecting oil-on-water based on a polarization analysis method. Its advantages include: a) no direct contact with sample water; b) minimal maintenance; c) largely unaffected by foreign matter and waves on the water surface; and d) much higher sensitivity than simple visual observation. This paper describes the measurement principle and configuration of the sensor system, and discusses the results of sensitivity tests and tests on the influence of water turbidity, foreign matter and waves. We will also consider some of the limitations of the new system.

Mixed methods for telehealth research

2016 ◽  
Vol 23 (9) ◽  
pp. 764-769 ◽  
Author(s):  
Liam J Caffery ◽  
Melinda Martin-Khan ◽  
Victoria Wade
Keyword(s):  
Mixed Methods ◽  
Mixed Methods Research ◽  
Complex Interventions ◽  
Evaluation Study ◽  
Assessment Tools ◽  
Quantitative Investigation ◽  
Services Research ◽  
Qualitative And Quantitative ◽  
Single Method ◽  
Study Designs

Mixed methods research is important to health services research because the integrated qualitative and quantitative investigation can give a more comprehensive understanding of complex interventions such as telehealth than can a single-method study. Further, mixed methods research is applicable to translational research and program evaluation. Study designs relevant to telehealth research are described and supported by examples. Quality assessment tools, frameworks to assist in the reporting and review of mixed methods research, and related methodologies are also discussed.

Effects of Sevoflurane on Excitatory Neurotransmission to Medullary Expiratory Neurons and on Phrenic Nerve Activity in a Decerebrate Dog Model

2001 ◽  
Vol 95 (2) ◽  
pp. 485-491 ◽  
Author(s):  
Astrid G. Stucke ◽  
Eckehard A. E. Stuth ◽  
Viseslav Tonkovic-Capin ◽  
Mislav Tonkovic-Capin ◽  
Francis A. Hopp ◽  
...  
Keyword(s):  
Neuronal Activity ◽  
Phrenic Nerve ◽  
Minimum Alveolar Concentration ◽  
Neuronal Response ◽  
Gamma Aminobutyric Acid ◽  
Nerve Activity ◽  
Gaba A ◽  
Phrenic Nerve Activity ◽  
Synaptic Neurotransmission ◽  
Expiratory Neurons

Background Sevoflurane is a new volatile anesthetic with a pronounced respiratory depressant effect. Synaptic neurotransmission in canine expiratory bulbospinal neurons is mainly mediated by excitatory N-methyl-D-aspartatic acid (NMDA) receptor input and modulated by inhibitory gamma-aminobutyric acid type A (GABA(A)) receptors. The authors investigated the effect of sevoflurane on these mechanisms in decerebrate dogs. Methods Studies were performed in decerebrate, vagotomized, paralyzed and mechanically ventilated dogs during hypercapnic hyperoxia. The effect of 1 minimum alveolar concentration (MAC; 2.4%) sevoflurane on extracellularly recorded neuronal activity was measured during localized picoejection of the glutamate agonist NMDA and the GABA(A) receptor blocker bicuculline in a two-part protocol. First, complete blockade of the GABA(A)ergic mechanism by bicuculline allowed differentiation between the effects of sevoflurane on overall GABA(A)ergic inhibition and on overall glutamatergic excitation. In a second step, the neuronal response to exogenous NMDA was used to estimate sevoflurane's effect on postsynaptic glutamatergic neurotransmission. Results One minimum alveolar concentration sevoflurane depressed the spontaneous activity of 16 expiratory neurons by 36.7+/-22.4% (mean +/- SD). Overall glutamatergic excitation was depressed 19.5+/-16.2%, and GABA(A)ergic inhibition was enhanced 18.7+/-20.6%. However, the postsynaptic response to exogenous NMDA was not significantly altered. In addition, 1 MAC sevoflurane depressed peak phrenic nerve activity by 61.8+/-17.7%. Conclusions In the authors' in vivo expiratory neuronal model, the depressive effect of sevoflurane on synaptic neurotransmission was caused by a reduction of presynaptic glutamatergic excitation and an enhancement of GABA(A)ergic inhibition. The effects on expiratory neuronal activity were similar to halothane, but sevoflurane caused a stronger depression of phrenic nerve activity than halothane.

scholarly journals Simultaneous Qualitative and Quantitative Evaluation of the Coptidis Rhizoma and Euodiae Fructus Herbal Pair by Using UHPLC-ESI-QTOF-MS and UHPLC-DAD

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4782
Author(s):  
Yan-Ying Li ◽  
Min-Qun Guo ◽  
Xue-Mei Li ◽  
Xiu-Wei Yang
Keyword(s):  
High Performance ◽  
Analysis Method ◽  
Chinese Medicines ◽  
Chemical Profiling ◽  
Qualitative And Quantitative ◽  
Flight Mass Spectrometry ◽  
Chinese Medicine Formula ◽  
Quantification Analysis ◽  
Coptidis Rhizoma ◽  
And Control

The herbal pair of Coptidis Rhizoma (CR) and Euodiae Fructus (EF) is a classical traditional Chinese medicine formula used for treating gastro-intestinal disorders. In this study, we established a systematic method for chemical profiling and quantification analysis of the major constituents in the CR-EF herbal pair. A method of ultra high performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) for qualitative analysis was developed. Sixty-five compounds, including alkaloids, phenolics, and limonoids, were identified or tentatively assigned by comparison with reference standards or literature data. The UHPLC fingerprints of 19 batches of the CR-EF herbal pair samples were obtained and the reference fingerprint chromatograms were established. Furthermore, nine compounds among 24 common peaks of fingerprints were considered as marker components, which either had high contents or significant bioactivities, were applied to quality control of the CR-EF herbal pair by quantitative analysis. This UHPLC-DAD analysis method was validated by precision, linearity, repeatability, stability, recovery, and so on. The method was simple and sensitive, and thus reliable for quantitative and chemical fingerprint analysis for the quality evaluation and control of the CR-EF herbal pair and related traditional Chinese medicines.

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