Protein Hydrolysis by Subcritical Water: A New Perspective on Obtaining Bioactive Peptides

The importance of bioactive peptides lies in their diverse applications in the pharmaceutical and food industries. In addition, they have been projected as allies in the control and prevention of certain diseases due to their associated antioxidant, antihypertensive, or hypoglycemic activities, just to mention a few. Obtaining these peptides has been performed traditionally by fermentation processes or enzymatic hydrolysis. In recent years, the use of supercritical fluid technology, specifically subcritical water (SW), has been positioned as an efficient and sustainable alternative to obtain peptides from various protein sources. This review presents and discusses updated research reports on the use of subcritical water to obtain bioactive peptides, its hydrolysis mechanism, and the experimental designs used for the study of effects from factors involved in the hydrolysis process. The aim was to promote obtaining peptides by green technology and to clarify perspectives that still need to be explored in the use of subcritical water in protein hydrolysis.

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Strategizing method optimization of microwave-assisted extraction of plant phenolics by developing standard working principles for universal robust optimization

Analytical Methods ◽

10.1039/c7ay00098g ◽

2017 ◽

Vol 9 (13) ◽

pp. 2089-2103 ◽

Cited By ~ 9

Author(s):

Harneet Kaur Kala ◽

Rajendra Mehta ◽

Kamal Kumar Sen ◽

Roshni Tandey ◽

Vivekananda Mandal

Keyword(s):

Global Optimization ◽

Robust Optimization ◽

Green Technology ◽

Microwave Assisted Extraction ◽

Plant Phenolics ◽

Food Industries ◽

Microwave Assisted ◽

Assisted Extraction ◽

Method Optimization

Empowering botanical extraction through microwave based green technology by proposing 8 golden principles for global optimization. A much needed boost for research in the pharma and food industries.

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Supercritical fluid technology for solubilization of poorly water soluble drugs via micro- and naonosized particle generation

ADMET & DMPK ◽

10.5599/admet.811 ◽

2020 ◽

Author(s):

Shashi Kiran Misra ◽

Kamla Pathak

Keyword(s):

Supercritical Fluid ◽

High Throughput Screening ◽

Green Technology ◽

Water Soluble ◽

Rapid Expansion ◽

Particle Generation ◽

Particulate Solids ◽

Supercritical Fluid Technology ◽

Water Soluble Drugs ◽

Poorly Water Soluble

<p class="ADMETabstracttext">Approximately two-third of the compounds in the pharmaceutical industry were developed through combinatorial chemistry and high throughput screening of particulate solids. Poor solubility and bioavailability of these pharmaceuticals are challenging attributes confronted by a formulator during product development. Hence, substantial efforts have been directed into the research on particle generation techniques. Although the conventional methods, such as crushing or milling and crystallization or precipitation, are still used; supercritical fluid technology introduced in the mid-1980s presents a new method of particle generation. Supercritical fluid processes not only produce micro- and nanoparticles with a narrow size distribution, they are also employed for the microencapsulation, cocrystallization, and surface coating with polymer. Recognized as a green technology, it has emerged as successful variants chiefly as Rapid Expansion of supercritical solutions (RESS), Supercritical anti-solvent (SAS) and Particles from Gas Saturated Solution (PGSS) depending upon type of solvent, solute, antisolvent and nebulization techniques. Being economical and eco-friendly, supercritical fluid technolgy has garnered considerable interest both in academia and industry for modification of physicochemical properties such as particle size, shape, density and ultimately solubility. The current manuscript is a comprehensive update on different supercritical fluid processes used for particle generation with the purpose of solubility enhancement of drugs and hence bioavailability.</p>

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Response Surface Approach for Optimization of Protein Hydrolysis from Reutealis trisperma Cake as Potential Animal Feedstock

REAKTOR ◽

10.14710/reaktor.20.1.26-31 ◽

2020 ◽

Vol 20 (1) ◽

pp. 26-31

Author(s):

Yuana Elly Agustin ◽

Lieke - Riadi ◽

Titie Prapti Utami

Keyword(s):

Protein Content ◽

Response Surface ◽

Maximum Yield ◽

Protein Yield ◽

Solvent Ratio ◽

Protein Hydrolysis ◽

Experimental Conditions ◽

Hydrolysis Process ◽

Mechanical Pressing ◽

Rsm Model

The conditions for protein hydrolysis were optimized to prepare Reutealis trisperma cake for potential animal feedstock. The cake’s content was 34.03 % protein, 6.32 % moisture, 18.56 % total sugar, 15.58 % lipid and 25.51% others. Other components in cake could be fibre and lignin. The cake is a byproduct of mechanical pressing process of the seeds and contains high protein content (34.03%). It was ground prior the hydrolysis process. A central composite design including concentration of NaOH, ratio of cake to NaOH, time and temperature were used to develop second order model to predict protein content under various experimental conditions. Protein yield was primarily affected by ratio pressed cake to NaOH and concentration of NaOH. Based on the Response Surface Methodology (RSM) model, maximum yield of protein was 11.33% which was obtained at cake/solvent ratio 1: 50; 1.5 % w/v NaOH; 15 minutes of hydrolysis at 40oC. The actual maximum protein yield from the experiment was obtained at cake/solvent ratio 1: 40; 1.5 % w/v NaOH; 20 minutes of hydrolysis at 45oC which was 21.28 %.Keywords: animal feedstock; protein hydrolysis; response surface; Reutealis trisperma

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Preparation, Identification, and Activity Evaluation of Eight Antioxidant Peptides from Protein Hydrolysate of Hairtail (Trichiurus japonicas) Muscle

Marine Drugs ◽

10.3390/md17010023 ◽

2019 ◽

Vol 17 (1) ◽

pp. 23 ◽

Cited By ~ 18

Author(s):

Xiu-Rong Yang ◽

Lun Zhang ◽

Dong-Ge Ding ◽

Chang-Feng Chi ◽

Bin Wang ◽

...

Keyword(s):

Superoxide Anion ◽

Protein Hydrolysate ◽

Reducing Power ◽

Model System ◽

Amino Acid Sequences ◽

Health Food ◽

Antioxidant Peptides ◽

Food Industries ◽

Molecular Weights ◽

Hydrolysis Process

In this report, protein of hairtail (Trichiurus japonicas) muscle was separately hydrolyzed using five kinds of proteases (alcalase, trypsin, neutrase, pepsin, and papain), and the papain- and alcalase-hydrolysates showed higher 2,2-diphenyl-1-picrylhydrazyl radicals (DPPH•) and hydroxyl radical (HO•) scavenging activity than other three protease hydrolysates. Therefore, the protein hydrolysate of hairtail muscle (HTP) was prepared using binary-enzymes hydrolysis process (papain + alcalase). Subsequently, eight antioxidant peptides were purified from HTP using membrane ultrafiltration and chromatography technology, and their amino acid sequences were identified as Gln-Asn-Asp-Glu-Arg (TJP1), Lys-Ser (TJP2), Lys-Ala (TJP3), Ala-Lys-Gly (TJP4), Thr-Lys-Ala (TJP5), Val-Lys (TJP6), Met-Lys (TJP7), and Ile-Tyr-Gly (TJP8) with molecular weights of 660.3, 233.0, 217.1, 274.1, 318.0, 245.1, 277.0, and 351.0 Da, respectively. TJP3, TJP4, and TJP8 exhibited strong scavenging activities on DPPH• (EC50 0.902, 0.626, and 0.663 mg/mL, respectively), HO• (EC50 1.740, 2.378, and 2.498 mg/mL, respectively), superoxide anion radical (EC50 2.082, 2.538, and 1.355 mg/mL, respectively), and 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical (EC50 1.652, 0.831, and 0.586 mg/mL, respectively). Moreover, TJP3, TJP4, and TJP8 showed higher reducing power and inhibiting ability on lipid peroxidation in a linoleic acid model system. These results suggested that eight isolated peptides (TJP1 to TJP8), especially TJP3, TJP4, and TJP8 might serve as potential antioxidants applied in the pharmaceutical and health food industries.

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Kinetic studies on rice bran protein hydrolysis in subcritical water

The Journal of Supercritical Fluids ◽

10.1016/j.supflu.2012.02.017 ◽

2012 ◽

Vol 65 ◽

pp. 54-60 ◽

Cited By ~ 22

Author(s):

Sasithorn Sunphorka ◽

Warinthorn Chavasiri ◽

Yoshito Oshima ◽

Somkiat Ngamprasertsith

Keyword(s):

Rice Bran ◽

Subcritical Water ◽

Kinetic Studies ◽

Protein Hydrolysis ◽

Rice Bran Protein

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Enhancing Enzymatic Digestibility of Coconut Husk using Nitrogen-assisted Subcritical Water for Sugar Production

BULLETIN OF CHEMICAL REACTION ENGINEERING AND CATALYSIS ◽

10.9767/bcrec.15.1.5337.84-95 ◽

2019 ◽

Vol 15 (1) ◽

pp. 84-95 ◽

Cited By ~ 1

Author(s):

Maktum Muharja ◽

Nur Fadhilah ◽

Tantular Nurtono ◽

Arief Widjaja

Keyword(s):

Enzymatic Hydrolysis ◽

Subcritical Water ◽

Agricultural Waste ◽

Biofuel Production ◽

Sugar Production ◽

Operation Condition ◽

Coconut Husk ◽

Commercial Scale ◽

Hydrolysis Process ◽

Hydrolysis Of

Coconut husk (CCH) as an abundant agricultural waste in Indonesia has the potential to be utilized for sugar production, which is the intermediate product of biofuel. In this study, subcritical water (SCW) assisted by nitrogen (N2) was developed to enhance the enzymatic hydrolysis of CCH. SCW process was optimized by varying the operation condition: the pressure of 60-100 bar, the temperature of 150-190 °C, and the time of 20-60 min. The SCW-treated solid was subsequently hydrolyzed by utilizing a mixture of commercial cellulase and xylanase enzymes. The result shows that the optimum total sugar yield was obtained under the mild condition of SCW treatment, resulting in the sugar of 15.67 % and 10.31 % gained after SCW and enzymatic hydrolysis process, respectively. SEM and FTIR analysis of SCW-treated solid exhibited the deformation of lignin and solubilization of cellulose and hemicellulose, while XRD and TGA revealed an increase of the amount of crystalline part in the solid residue. The use of N2 in SCW treatment combined with enzymatic hydrolysis in this study suggested that the method can be considered economically for biofuel production from CCH waste in commercial scale. Copyright © 2020 BCREC Group. All rights reserved

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Influence of the rapeseed protein hydrolysis process on CHO cell growth

Bioresource Technology ◽

10.1016/j.biortech.2007.12.070 ◽

2008 ◽

Vol 99 (15) ◽

pp. 7143-7151 ◽

Cited By ~ 18

Author(s):

G. Chabanon ◽

L. Alves da Costa ◽

B. Farges ◽

C. Harscoat ◽

S. Chenu ◽

...

Keyword(s):

Cell Growth ◽

Protein Hydrolysis ◽

Cho Cell ◽

Rapeseed Protein ◽

Hydrolysis Process

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Identification of antioxidant and antibacterial activities for the bioactive peptides generated from bitter beans (Parkia speciosa) via boiling and fermentation processes

LWT ◽

10.1016/j.lwt.2020.109776 ◽

2020 ◽

Vol 131 ◽

pp. 109776

Author(s):

Belal J. Muhialdin ◽

Nur Fatin Abdul Rani ◽

Anis Shobirin Meor Hussin

Keyword(s):

Bioactive Peptides ◽

Antibacterial Activities ◽

Fermentation Processes

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Study on Milk Protein Hydrolysis of Infant Formula

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.898.326 ◽

2014 ◽

Vol 898 ◽

pp. 326-329

Author(s):

Li Fu ◽

Xi Qing Yue ◽

Jian Xin Song

Keyword(s):

Infant Formula ◽

Milk Protein ◽

Whey Proteins ◽

Enzyme Linked Immunosorbent Assay ◽

Protein Hydrolysis ◽

Step Process ◽

Hydrolysis Process ◽

One Step ◽

Β Lactoglobulin ◽

Hydrolysis Of

First of all, whey protein concentrat was added to milk adjusting the proportion of casein and whey proteins to 40:60. And then milk was hydrolyzed by trypsin and flavourzyme (TF) single respectively in one-step process or staged in two-step process. The Antigen contents of α-lactalbumin (α-LA) and β-lactoglobulin (β-LG) during the hydrolysis process were determined by indirect competitive enzyme-linked immunosorbent assay (ELISA) methods. The result showed that the most significant antigen reduction was observed in two-step hydrolysis process compared with one-step hydrolysis process

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