Cerium Oxide Nanoparticles and Their Efficient Antibacterial Application In Vitro against Gram-Positive and Gram-Negative Pathogens

In this study, the antibacterial activity of cerium oxide nanoparticles on two Gram-negative and three Gram-positive foodborne pathogens was investigated. CeO2 nanoparticles (CeO2 nps) were synthesized by a Wet Chemical Synthesis route, using the precipitation method and the Simultaneous Addition of reactants (WCS–SimAdd). The as-obtained precursor powders were investigated by thermal analysis (TG–DTA), to study their decomposition process and to understand the CeO2 nps formation. The composition, structure, and morphology of the thermally treated sample were investigated by FTIR, Raman spectroscopy, X-ray diffraction, TEM, and DLS. The cubic structure and average particle size ranging between 5 and 15 nm were evidenced. Optical absorption measurements (UV–Vis) reveal that the band gap of CeO2 is 2.61 eV, which is smaller than the band gap of bulk ceria. The antioxidant effect of CeO2 nps was determined, and the antibacterial test was carried out both in liquid and on solid growth media against five pathogenic microorganisms, namely Escherichia coli, Salmonella typhimurium, Listeria monocytogenes, Staphylococcus aureus, and Bacillus cereus. Cerium oxide nanoparticles showed growth inhibition toward all five pathogens tested with notable results. This paper highlights the perspectives for the synthesis of CeO2 nps with controlled structural and morphological characteristics and enhanced antibacterial properties, using a versatile and low-cost chemical solution method.

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Precise engineering of ultrasound-mediated cerium oxide nanoparticles: Investigations on antioxidant, antibacterial activity and human osteomyelitis

10.21203/rs.3.rs-238469/v1 ◽

2021 ◽

Author(s):

Xiusong Dai ◽

Xiaopan Wang ◽

Xiaotian Chen ◽

Longfei Ye ◽

Min Wu

Keyword(s):

Antibacterial Activity ◽

Band Gap ◽

Cerium Oxide ◽

Foodborne Pathogens ◽

Average Particle Size ◽

Precipitation Method ◽

Cerium Oxide Nanoparticles ◽

Growth Media ◽

Oxide Nanoparticles ◽

Average Particle

Abstract In this study, the antibacterial activity of cerium oxide nanoparticles on two Gram-negative and three Gram-positive foodborne pathogens was investigated. CeO2 nanoparticles (CeO2 NPs) were synthesized by a Wet Chemical Synthesis route, using the precipitation method and the Simultaneous Addition of reactants (WCS–SimAdd). The as-obtained precursor powders were investigated by thermal analysis (TG–DTA), to study their decomposition process and to understand the CeO2 NPs formation. The composition, structure, and morphology of the thermally treated sample were investigated by FTIR, Raman spectroscopy, X-ray diffraction, TEM, and DLS. The cubic structure and average particle size ranging between 5 and 15 nm were evidenced. Optical absorption measurements (UV–Vis) reveal that the band gap of CeO2 NPs is 2.61 eV, which is smaller than the band gap of bulk ceria. The antioxidant effect of CeO2 NPs was determined, and the antibacterial test was carried out both in liquid and on solid growth media against five pathogenic microorganisms, namely Escherichia coli, Salmonella typhimurium, Listeria monocytogenes, Staphylococcus aureus, and Bacillus cereus. Cerium oxide nanoparticles showed growth inhibition toward all five pathogens tested with notable results. This paper highlights the CeO2 NPs showed antibacterial activity with significant variations due to the differences in the membrane structure and cell wall composition among the two groups tested. Consequently, synthesized CeO2 NPs can be potential candidates for the treatment of osteomyelitis.

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Cerium Oxide Nanoparticles Improve Lifespan of Stored Blood

Military Medicine ◽

10.1093/milmed/usz210 ◽

2020 ◽

Vol 185 (Supplement_1) ◽

pp. 103-109

Author(s):

Beverly A Rzigalinski ◽

Holly M Giovinco ◽

Byron J Cheatham

Keyword(s):

Oxidative Stress ◽

Cerium Oxide ◽

Whole Blood ◽

Average Particle Size ◽

Oxygen Storage ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Average Particle ◽

Stored Blood

ABSTRACT Introduction Blood is a precious commodity, with storage limited to 42 days under refrigeration. Degradative changes in red blood cells (RBCs) begin as early as 11–21 days after collection, and compromise their function. Materials that extend the life of RBCs will improve blood utilization in the field, as well as in hospital settings. Cerium oxide nanoparticles (CeONPs) are widely used in the materials industry to counteract oxidative stress and improve oxygen storage. We have previously shown that CeONPs extended the lifespan of cells in culture and counteract oxidative stress in vitro and in vivo. Here, we test the hypothesis that CeONPs extend the lifespan of RBCs in whole stored blood. Materials and Methods Rat whole blood was collected with sodium citrate and stored at 4°C. Groups consisted of control (no CeONPs), and 10 and 100 nM CeONPs (average particle size 10 nm) added. Aliquots of stored blood were removed weekly and analyzed for different blood parameters. Results Results demonstrate that CeONPs improve storage and functional lifespan of RBCs in stored whole blood. Conclusions This work suggests that CeONPs may be a promising additive for extending storage and function of blood and blood products.

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Emulsification and application of a thymol loaded antibacterial fatliquor for leather industry

Journal of Industrial Textiles ◽

10.1177/1528083720941117 ◽

2020 ◽

pp. 152808372094111

Author(s):

Ali Yorgancioglu

Keyword(s):

Particle Size ◽

Average Particle Size ◽

Antibacterial Properties ◽

Test Method ◽

Average Particle ◽

Gram Negative Bacteria ◽

Bacterial Reduction ◽

Leather Industry ◽

Gram Positive ◽

Gram Negative

In this study, the antibacterial fatliquor emulsion was prepared from castor oil bearing thymol (1, 2, 4 and 8% w/w) to be used in the leather production. The average particle size and zeta potential of the fatliquors were determined by zeta sizer. The thermogravimetric behaviors of fatliquor emulsions under dry air were analyzed in order to determine the weight losses when they are exposed to constant heating rate. After the emulsification process, fatliquor emulsions were applied into chrome tanned cattle leathers. Antibacterial activities of the fatliquored leather were tested according to standard test method for determining the antimicrobial activity under dynamic contact conditions against Gram-positive and Gram-negative bacteria. Minimum inhibitory concentration (MIC) analysis of emulsion was also determined. The bacterial reduction was measured as 99.9% against S. aureus and 98.6% against E. coli bacteria. MIC values obtained from emulsions against bacteria were determined as 6.25 μL/mL and 12.5 μL/mL respectively. The results of antibacterial tests showed that thymol was very efficient in the fatliquor emulsion against both Gram-positive bacteria and Gram-negative bacteria. Physical characterization of leather was also carried out with optimum fatliquor emulsion (4% thymol w/w) and the results showed satisfactory physical properties on the fatliquored leathers compared to similar leather made with commercially available fatliquors. The results of the study indicated that thymol loaded fatliquor emulsions with small particle size could be a promising solo fatliquor to provide functional properties such as physical, strength, morphological and antibacterial properties to leather as an efficient fatliquor compared to conventional fatliquors.

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Dissolution and transformation of cerium oxide nanoparticles in plant growth media

Journal of Nanoparticle Research ◽

10.1007/s11051-014-2668-8 ◽

2014 ◽

Vol 16 (10) ◽

Cited By ~ 49

Author(s):

Franziska Schwabe ◽

Rainer Schulin ◽

Patrick Rupper ◽

Aline Rotzetter ◽

Wendelin Stark ◽

...

Keyword(s):

Plant Growth ◽

Cerium Oxide ◽

Cerium Oxide Nanoparticles ◽

Growth Media ◽

Oxide Nanoparticles

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Functional Properties of Sonochemically Synthesized Zinc Oxide Nanoparticles and Cotton Composites

Nanomaterials ◽

10.3390/nano10091661 ◽

2020 ◽

Vol 10 (9) ◽

pp. 1661 ◽

Cited By ~ 3

Author(s):

Muhammad Tayyab Noman ◽

Michal Petrů

Keyword(s):

Zinc Oxide ◽

Zinc Oxide Nanoparticles ◽

Average Particle Size ◽

Antimicrobial Properties ◽

Acoustic Method ◽

Oxide Nanoparticles ◽

Average Particle ◽

Gram Positive ◽

X Ray ◽

Antimicrobial Performance

In this study, zinc oxide nanoparticles (nZnO) were synthesized, deposited, and successfully used for surface modification of cotton to enhance antimicrobial properties. An in situ ultrasonic acoustic method was applied to anchor nZnO on cotton. The results of scanning electron microscopy, energy dispersive X-ray spectroscopy, and X-ray diffraction confirmed the presence of nZnO on cotton. A homogenous distribution of nZnO with an average particle size 27.4 nm was found during the analysis of results. Antimicrobial performance of cotton-nZnO (C-nZnO) composites was evaluated against Gram-negative and Gram-positive microbes. The deposited amount of nZnO on C-nZnO composites was determined by volumetric titration through inductive couple plasma atomic emission spectroscopy. C-nZnO composites showed excellent antimicrobial performance especially against both Staphylococcus aureus (Gram-positive) and Escherichia coli. The durability and stability of C-nZnO composites were tested against leaching and washing. No significant fluctuation was found on deposited amount of nZnO before and after washing test for optimized sample. The results demonstrate that synthesized C-nZnO composite samples can be used as an alternative for antimicrobial bandages.

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Revealing the Effect of Synthesis Conditions on the Structural, Optical, and Antibacterial Properties of Cerium Oxide Nanoparticles

Nanomaterials ◽

10.3390/nano11102596 ◽

2021 ◽

Vol 11 (10) ◽

pp. 2596

Author(s):

Nicusor Fifere ◽

Anton Airinei ◽

Marius Dobromir ◽

Liviu Sacarescu ◽

Simona I. Dunca

Keyword(s):

Cerium Oxide ◽

Diffuse Reflectance ◽

Diffuse Reflectance Spectroscopy ◽

Antibacterial Properties ◽

Morphological Characteristics ◽

Precipitation Method ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

X Ray ◽

Gap Energy

Cerium oxide nanoparticles were prepared by a precipitation method using Ce(IV) sulphate as precursor dispersed in glycerol with varying synthesis parameters such as temperature or precipitating agent. The structural and morphological characteristics of the obtained nanoparticles were investigated by X-ray diffraction, transmission electron microscopy, and diffuse reflectance spectroscopy. The crystallite size of the nanoparticles varied between 13 and 17 nm. The presence of Ce3+ and Ce4+ was proved by XPS data in the CeO2 samples and the conservation of the fluorite structure was evinced by X-ray diffractograms with a contraction of the lattice parameter, regardless of the size of the nanoparticle. From diffuse reflectance spectra, two band gap energy values for the direct transition were observed. Depending on the synthesis condition, the red shift of gap energy and the blue shift of Urbach energy with increasing content of Ce3+ were ascertained. The antibacterial tests revealed that the cerium oxide nanoparticles show good antimicrobial activity towards the common pathogens Escherichia coli and Staphylococcus aureus.

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Effects of Engineered Cerium Oxide Nanoparticles on Bacterial Growth and Viability

Applied and Environmental Microbiology ◽

10.1128/aem.00650-10 ◽

2010 ◽

Vol 76 (24) ◽

pp. 7981-7989 ◽

Cited By ~ 213

Author(s):

Dale A. Pelletier ◽

Anil K. Suresh ◽

Gregory A. Holton ◽

Catherine K. McKeown ◽

Wei Wang ◽

...

Keyword(s):

Cerium Oxide ◽

Transcriptional Profiling ◽

Shewanella Oneidensis ◽

Oxide Particle ◽

Conservation Strategies ◽

Cerium Oxide Nanoparticles ◽

Growth Media ◽

Oxide Nanoparticles ◽

Analytical Approaches ◽

Bacterial Systems

ABSTRACT Interest in engineered nanostructures has risen in recent years due to their use in energy conservation strategies and biomedicine. To ensure prudent development and use of nanomaterials, the fate and effects of such engineered structures on the environment should be understood. Interactions of nanomaterials with environmental microorganisms are inevitable, but the general consequences of such interactions remain unclear, due to a lack of standard methods for assessing such interactions. Therefore, we have initiated a multianalytical approach to understand the interactions of synthesized nanoparticles with bacterial systems. These efforts are focused initially on cerium oxide nanoparticles and model bacteria in order to evaluate characterization procedures and the possible fate of such materials in the environment. The growth and viability of the Gram-negative species Escherichia coli and Shewanella oneidensis, a metal-reducing bacterium, and the Gram-positive species Bacillus subtilis were examined relative to cerium oxide particle size, growth media, pH, and dosage. A hydrothermal synthesis approach was used to prepare cerium oxide nanoparticles of defined sizes in order to eliminate complications originating from the use of organic solvents and surfactants. Bactericidal effects were determined from MIC and CFU measurements, disk diffusion tests, and live/dead assays. For E. coli and B. subtilis, clear strain- and size-dependent inhibition was observed, whereas S. oneidensis appeared to be unaffected by the particles. Transmission electron microscopy along with microarray-based transcriptional profiling was used to understand the response mechanism of the bacteria. Use of multiple analytical approaches adds confidence to toxicity assessments, while the use of different bacterial systems highlights the potential wide-ranging effects of nanomaterial interactions in the environment.

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Spinel-Type Cobalt Oxide Nanoparticles: Synthesis, Characterization and Antibacterial Properties

Advanced Science Engineering and Medicine ◽

10.1166/asem.2020.2623 ◽

2020 ◽

Vol 12 (7) ◽

pp. 859-863

Author(s):

S. Ambika ◽

S. Gopinath ◽

K. Sivakumar ◽

K. Saravanan

Keyword(s):

Electron Microscopy ◽

Cobalt Oxide ◽

Average Particle Size ◽

Antibacterial Properties ◽

Synthesis Temperature ◽

Oxide Nanoparticles ◽

Average Particle ◽

Spinel Type ◽

Cobalt Oxide Nanoparticles ◽

The Impact

A series of cobalt oxide nanoparticles (sample A (300 °C), sample B (400 °C) and sample C (500 °C)) are synthesized in a starch medium by the hydrothermal method followed by annealing. The structural, morphological, and magnetic, of these cobalt oxide nanoparticles, become characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), vibrating sample magnetometer (VSM) correspondingly. Furthermore, it can be seen that the increase in the synthesis temperature results in a substantial increase in the average particle size. The impact of synthesis temperature in relation to morphology (size and shape) and magnetic properties of cobalt oxide nanoparticles has been investigated. Besides, the antibacterial and catalytic activity of these nanoparticles are examined. A systematic analysis is presented on the influence of parameters of synthesis on the formation and structural properties of cobalt oxide nanoparticles. We expect in which this topic will give many valuable ideas for the metal oxide nanomaterials development, synthesis, as well as applications.

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