scholarly journals Cyclodextrin Cationic Polymer-Based Nanoassemblies to Manage Inflammation by Intra-Articular Delivery Strategies

Nanomaterials ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1712
Author(s):  
Annalaura Cordaro ◽  
Roberto Zagami ◽  
Milo Malanga ◽  
Jagadeesh Kumar Venkatesan ◽  
Carmen Alvarez-Lorenzo ◽  
...  
Keyword(s):  
Release Kinetics ◽  
Supramolecular Interactions ◽  
Spectroscopic Techniques ◽  
Cationic Polymers ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Potential Measurement ◽  
Diagnostic Agents ◽  
Delivery Strategies ◽  
Anti Inflammatory

Injectable nanobioplatforms capable of locally fighting the inflammation in osteoarticular diseases, by reducing the number of administrations and prolonging the therapeutic effect is highly challenging. β-Cyclodextrin cationic polymers are promising cartilage-penetrating candidates by intra-articular injection due to the high biocompatibility and ability to entrap multiple therapeutic and diagnostic agents, thus monitoring and mitigating inflammation. In this study, nanoassemblies based on poly-β-amino-cyclodextrin (PolyCD) loaded with the non-steroidal anti-inflammatory drug diclofenac (DCF) and linked by supramolecular interactions with a fluorescent probe (adamantanyl-Rhodamine conjugate, Ada-Rhod) were developed to manage inflammation in osteoarticular diseases. PolyCD@Ada-Rhod/DCF supramolecular nanoassemblies were characterized by complementary spectroscopic techniques including UV-Vis, steady-state and time-resolved fluorescence, DLS and ζ-potential measurement. Stability and DCF release kinetics were investigated in medium mimicking the physiological conditions to ensure control over time and efficacy. Biological experiments evidenced the efficient cellular internalization of PolyCD@Ada-Rhod/DCF (within two hours) without significant cytotoxicity in primary human bone marrow-derived mesenchymal stromal cells (hMSCs). Finally, polyCD@Ada-Rhod/DCF significantly suppressed IL-1β production in hMSCs, revealing the anti-inflammatory properties of these nanoassemblies. With these premises, this study might open novel routes to exploit original CD-based nanobiomaterials for the treatment of osteoarticular diseases.

Interaction of human serum albumin with liposomes of saturated and unsaturated lipids with different phase transition temperatures: a spectroscopic investigation by membrane probe PRODAN

RSC Advances ◽  
2014 ◽  
Vol 4 (28) ◽  
pp. 14335-14347 ◽  
Author(s):  
Raina Thakur ◽  
Anupam Das ◽  
Anjan Chakraborty
Keyword(s):  
Phase Transition ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Spectroscopic Techniques ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Unsaturated Lipids ◽  
Membrane Probe ◽  
Phase Transition Temperatures

The interaction of human serum albumin (HSA) with liposomes made of saturated and unsaturated phosphocholines has been studied using circular dichroism (CD), steady state and time resolved fluorescence spectroscopic techniques.

scholarly journals Time-resolved fluorescence anisotropy as a tool to study guest–cucurbit[n]uril–protein ternary supramolecular interactions

2015 ◽  
Vol 14 (4) ◽  
pp. 842-852 ◽  
Author(s):  
Karina Scholtbach ◽  
Ítalo Venegas ◽  
Cornelia Bohne ◽  
Denis Fuentealba
Keyword(s):  
Fluorescence Anisotropy ◽  
Model System ◽  
Supramolecular Interactions ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
System P

A combined time-resolved fluorescence and anisotropy analysis was used to study guest–cucurbit[n]uril–protein ternary supramolecular interactions in a model system.

Excited state proton transfer dynamics of an eminent anticancer drug, ellipticine, in octyl glucoside micelle

2014 ◽  
Vol 16 (28) ◽  
pp. 14953-14960 ◽  
Author(s):  
Krishna Gavvala ◽  
Raj Kumar Koninti ◽  
Abhigyan Sengupta ◽  
Partha Hazra
Keyword(s):  
Excited State ◽  
Steady State ◽  
Proton Transfer ◽  
Anticancer Drug ◽  
Photophysical Properties ◽  
Spectroscopic Techniques ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Octyl Glucoside ◽  
Excited State Proton Transfer

Prototropical and photophysical properties of an anticancer drug, ellipticine, are explored inside the octyl-β-d-glucoside micelles using steady state and time-resolved fluorescence spectroscopic techniques.

Spectroscopic and theoretical insights on determination of binding strength and molecular structure for the supramolecular complexes of a designed bisporphyrin with C60 and C70

2012 ◽  
Vol 16 (01) ◽  
pp. 14-24 ◽  
Author(s):  
Partha Mukherjee ◽  
Subarata Chattopadhyay ◽  
Sumanta Bhattacharya
Keyword(s):  
Molecular Electrostatic Potential ◽  
Binding Constants ◽  
Spectroscopic Techniques ◽  
Time Resolved Fluorescence ◽  
Nmr Studies ◽  
Time Resolved ◽  
Average Value ◽  
Molecular Tweezer ◽  
Molecular Electrostatic Potential Maps

The present article examines the binding affinity of the newly designed Zn2-bisporphyrin molecule, syn-1, towards C60 and C70 in toluene medium. The investigation is carried out by UV-vis spectrophotometric, steady state and time-resolved fluorescence spectroscopic techniques. The bisporphyrin, syn-1, serves as an effective and selective molecular tweezer for C70 as average value of binding constants (K) for the non-covalent complexes of syn-1 with C60 and C70 are estimated to be 1.65 × 104 and 1.05 × 105 dm3 · mol-1, respectively. Binding of C70 in the cleft of syn-1 is clearly demonstrated by the quantum chemical calculations at ab initio level of theory. Molecular electrostatic potential maps demonstrate significant redistribution of charges in these supramolecules. Proton NMR studies suggest that the C70 moiety remains at the shallow part of the cleft of syn-1.

Diterpenes from Euphorbia myrsinites and Their Anti-inflammatory Property

Planta Medica ◽  
2021 ◽  
Author(s):  
Laura Grauso ◽  
Bruna de Falco ◽  
Giuseppe Lucariello ◽  
Raffaele Capasso ◽  
Virginia Lanzotti
Keyword(s):  
Folk Medicine ◽  
Phytochemical Analysis ◽  
Spectroscopic Techniques ◽  
Significant Activity ◽  
Relative Configuration ◽  
Drug Candidates ◽  
The Usa ◽  
Anti Inflammatory ◽  
Dose Dependent ◽  
Diterpene Alcohol

Abstract Euphorbia myrsinites is one of the oldest spurges described and used in folk medicine. It is characterized by blue-grey stems similar to myrtle, and it is spread in the Mediterranean region, Asia, and the USA. Chemical analysis of E. myrsinites collected in Turkey afforded the isolation of 4 diterpenes based on the so-called myrsinane skeleton being tetraesters of the tetracyclic diterpene alcohol myrsinol. In this study, the phytochemical analysis of this species collected in Italy has been undertaken to afford the isolation of a new atisane diterpene, named myrsatisane, 3 ingenol derivatives, along with the 4 tetraester derivatives previously found. A triterpene compound based on the euphane skeleton has also been isolated. Structural elucidation of the new myrsatisane was based on spectroscopic techniques, including HR-MS and 1- and 2-dimensional NMR experiments. Its relative configuration was determined by NOE correlations, while absolute stereochemistry was obtained by quantum-mechanical DFT studies. While diterpenes with the atisane skeleton are relatively common in Euphorbia species, this is the first report of an atisane diterpene from E. myrsinites. All the isolated terpenes were tested for anti-inflammatory activity on J774A.1 macrophages stimulated with lipopolysaccharide by evaluation of nitrite and pro-inflammatory cytokine Il-1β levels. Among tested compounds, the 3 ingenol diterpenes exhibited a dose-dependent (0.001 – 3 µM) significant activity, thus showing their potential as anti-inflammatory drug candidates.

scholarly journals The influence of sugar–protein complexes on the thermostability of C-reactive protein (CRP)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Andreea Lorena Mateescu ◽  
Nicolae-Bogdan Mincu ◽  
Silvana Vasilca ◽  
Roxana Apetrei ◽  
Diana Stan ◽  
...  
Keyword(s):  
Diagnostic Tests ◽  
Protein Complexes ◽  
C Reactive Protein ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Reactive Protein ◽  
In Vitro Diagnostic ◽  
The Stability ◽  
Positive Controls

AbstractThe purpose of the present study was to evaluate de influence of protein–sugar complexation on the stability and functionality of C-reactive protein, after exposure to constant high temperatures, in order to develop highly stable positive controls for in-vitro diagnostic tests. C-reactive protein is a plasmatic protein used as a biomarker for the diagnosis of a series of health problems such as ulcerative colitis, cardiovascular diseases, metabolic syndrome, due to its essential role in the evolution of chronic inflammation. The sugar–protein interaction was investigated using steady state and time resolved fluorescence. The results revealed that there are more than two classes of tryptophan, with different degree of accessibility for the quencher molecule. Our study also revealed that sugar–protein complexes have superior thermostability, especially after gamma irradiation at 2 kGy, the protein being stable and functional even after 22 days exposure to 40 °C.

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