Cetyltrimethylammonium Bromide (CTAB)-Loaded SiO2–Ag Mesoporous Nanocomposite as an Efficient Antibacterial Agent

To date, Ag-based nanomaterials have demonstrated a high potential to overcome antibiotic resistance issues. However, bare Ag nanomaterials are prone to agglomeration in the biological environment, which results in a loss of antibacterial activity over time. Furthermore, it is still challenging to collect small-sized Ag nanomaterials right after the synthesis process. In this study, spherical-shaped Ag nanoparticles (NPs) (~6–10 nm) were attached on the surface of cetyltrimethylammonium bromide (CTAB)-loaded mesoporous silica nanoparticles (MSNs) (~100–110 nm). Antibacterial activity tests suggested that the obtained nanocomposite can be used as a highly efficient antibacterial agent against both Gram-negative and Gram-positive bacterial strains. The minimum inhibitory concentration (MIC) recalculated to pure Ag weight in nanocomposite was found to be ~1.84 µg/mL (for Escherichia coli) and ~0.92 µg/mL (for Staphylococcus aureus)—significantly smaller compared to values reported to date. The improved antibacterial activity of the prepared nanocomposite can be attributed to the even distribution of non-aggregated Ag NPs per volume unit and the presence of CTAB in the nanocomposite pores.

Download Full-text

Antibacterial Activity Inhibition after the Degradation of Flumequine by UV/H2O2

Journal of Advanced Oxidation Technologies ◽

10.1515/jaots-2011-0113 ◽

2011 ◽

Vol 14 (1) ◽

Cited By ~ 1

Author(s):

Caio Rodrigues da Silva ◽

Milena Guedes Maniero ◽

Susanne Rath ◽

José Roberto Guimarães

Keyword(s):

Antibacterial Activity ◽

Broad Spectrum ◽

Antibacterial Agent ◽

Veterinary Drug ◽

Drug Resistant ◽

Bacterial Strains ◽

Activity Inhibition

AbstractFlumequine is a broad-spectrum antibacterial agent of the quinolone class widely used as veterinary drug in food-producing animals. It is considered as pseudo-persistent compound continuously introduced into the environment and its presence in the environment may contribute to the development of drug resistant bacterial strains. In this study antibacterial activity removal during flumequine degradation by UV/H

Download Full-text

Endophytic Actinobacteria from Rhododendron spp. as an Antibacterial Agent

Biosaintifika Journal of Biology & Biology Education ◽

10.15294/biosaintifika.v9i3.10323 ◽

2017 ◽

Vol 9 (3) ◽

pp. 600

Author(s):

Risa Fitriandini ◽

Sri Budiarti ◽

Yulin Lestari

Keyword(s):

Antibacterial Activity ◽

Antibacterial Agent ◽

Bacillus Pumilus ◽

Inhibitory Concentration ◽

Bacterial Pathogen ◽

Inhibition Zone ◽

Cell Number ◽

High Antibacterial Activity ◽

Mic Value ◽

Endophytic Actinobacteria

Rhododendron has long been known to treat various diseases including diarrhea, but diversity and potency of its endophytic actinobacteria has not been studied. The objectives of this research were to explore the existence of endophytic actinobacteria from Rododendron spp. and assesed their antibacterial activity, as an effort to control the growth of bacterial pathogen resistant to some antibiotics. The endophytes were isolated from Rhododendron spp. using HV medium, and purified in ISP2 medium. The antibacterial activity was assayed against Enteropathogenic Escerichia coli (EPEC) K1.1 resistant to ampicillin and Bacillus pumilus. The Minimum Inhibitory Concentration (MIC) value, macroscopic and microscopic were examined. Twenty three of endophytic actinobacteria were successfully isolated from 7 Rhododendron species. Two of them, i.e. RJkb1 and RJkb3 isolates, had high antibacterial activity, with 17.2 mm and 14.5 mm inhibition zone against EPEC K1-1, respectively; and 12.4 mm and 16.1 mm inhibition zone against B. pumilus, respectively. The highest antibacterial activity for both RJkb1 and RJkb3 isolates was achieved at day 15, at 28 oC. At 250 µg/mL to 1750 µg/mL either RJkb1 or RJkb3 supernatant showed no activity against EPEC K1-1. The MIC value against B. pumilus was at 1250 µg/mL for both tested isolates. Under an electron microscope observation, cell morphology of the treated B. pumilus showed elongated cells and viewer in cell number, compared with the untreated one. From this work, the existence of endophytic actinobacteria from Rhododendron spp. and their antibacterial activity contribute to the understanding of their diversity and potency as antibacterial agent.

Download Full-text

STUDY CYTOTOXICITY, ANTIBACTERIAL ACTIVITY, AND COMPOSITIONS BY FT-IR AND GC-MS FOR ORGANIC EXTRACT OF WHITE IRAQI DESERT TRUFFLE TIRMANIA NIVEA

Periódico Tchê Química ◽

10.52571/ptq.v17.n36.2020.34_periodico36_pgs_18_31.pdf ◽

2020 ◽

Vol 17 (36) ◽

pp. 18-31

Author(s):

Ahmad khadem HACHIM ◽

Rashid Rahim HATEET ◽

Tawfik Muhammad MUHSIN

Keyword(s):

Antibacterial Activity ◽

Minimum Inhibitory Concentration ◽

Cell Line ◽

Pathogenic Bacteria ◽

Inhibitory Concentration ◽

Biological Activities ◽

Gas Chromatography Mass Spectrometry ◽

Bacterial Strains ◽

Organic Extract

The purpose of the present work aimed at exploring the potential biochemical components and biological activities of an organic extract of the white truffle Tirmania nivea collected from the Iraqi desert, then test the organic extract against the Cytotoxicity on Human Larynx carcinoma cells and selected strains of pathogenic bacteria. Fourier transform infrared spectroscopy (FTIR) and gas chromatography-mass spectrometry GC/MSS were used to analyze mycochemical compositions. The antibacterial activity and Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) was investigated using a disk diffusion agar method. The truffle extract's cytotoxicity effect against the larynx cell line (Hep-2) was assessed by the MTT assay (in vitro). FTIR results provided the presence of phenol, carboxylic acid, and alkane's functional group, The GC-MS analysis of T. nivea disclose the existence of nineteen compounds that can contribute to the pharmaceutical properties of the truffle. As for antibacterial activity result, A growth inhibition activity of truffle extract at (18-40 mm inhibition zones) against the tested pathogenic bacterial strains was detected, which minimum inhibitory concentration values ranged from 3.12 to 6.25 mg/mL for Escherichia coli (ATCC 25922) and Staphylococcus aureus (ATCC 25923) Respectively. The results of cytotoxicity shown that the organic truffle extract exhibited a high inhibitory rate (52.685%) against cell line (Hep-2) at a concentration of 1.56 ?g/mL. In this work, the results showed that the organic extracts of T. nivea are very promising as cancer cytotoxicity and antibacterial agent for future medical applications.

Download Full-text

Antibacterial and antioxidant activities of Licania tomentosa (Benth.) fritsch (crhysobalanaceae)

Archives of Biological Sciences ◽

10.2298/abs1202459s ◽

2012 ◽

Vol 64 (2) ◽

pp. 459-464 ◽

Cited By ~ 3

Author(s):

J.B.N.F. Silva ◽

I.R.A. Menezes ◽

H.D.M. Coutinho ◽

F.F.G. Rodrigues ◽

J.G.M. Costa ◽

...

Keyword(s):

Antibacterial Activity ◽

Secondary Metabolites ◽

Inhibitory Concentration ◽

Antioxidant Activities ◽

Positive Control ◽

Antioxidative Capacity ◽

Bacterial Strains ◽

Hydroalcoholic Extract ◽

Phytochemical Composition ◽

Antibacterial Assay

This work describes the chemical composition, and evaluates the antimicrobial and antioxidant activities of a hydroalcoholic extract from the leaves of the Licania tomentosa. Gram positive and negative bacterial strains were used in this work. Examination of the phytochemical composition of L. tomentosa revealed the presence of secondary metabolites such as tannins, flavonoids, saponins, alkaloids, steroids and triterpenoids. An antibacterial assay pointed out that the extract had a lower minimal inhibitory concentration (MIC - 32 ?g/mL) towards Staphylococcus aureus (ATCC12692). The extract also presented antibacterial activity against other assayed bacteria, with the MIC varying between 64 and 512 ?g/ mL. Our findings reveal that the extract presented an antioxidative capacity lower than that of BHT at the same concentration, used as positive control. Our results suggest that the levels and combinations between the secondary metabolites of this plant should be investigated to explain the demonstrated antibacterial activity.

Download Full-text

Selenocompounds as Novel Antibacterial Agents and Bacterial Efflux Pump Inhibitors

Molecules ◽

10.3390/molecules24081487 ◽

2019 ◽

Vol 24 (8) ◽

pp. 1487 ◽

Cited By ~ 7

Author(s):

Tímea Mosolygó ◽

Annamária Kincses ◽

Andrea Csonka ◽

Ádám Szabó Tönki ◽

Karolina Witek ◽

...

Keyword(s):

Antibacterial Activity ◽

Multidrug Resistance ◽

Carbonyl Compounds ◽

Antibacterial Agents ◽

Inhibitory Concentration ◽

Efflux Pump ◽

Attractive Potential ◽

Bacterial Strains ◽

Gram Positive Bacteria ◽

Efflux Pump Inhibitors

Bacterial multidrug resistance is becoming a growing problem for public health, due to the development and spreading of bacterial strains resistant to antimicrobials. In this study, the antibacterial and multidrug resistance reversing activity of a series of seleno-carbonyl compounds has been evaluated. The effects of eleven selenocompounds on bacterial growth were evaluated in Staphylococcus aureus, methicillin resistant S. aureus (MRSA), Enterococcus faecalis, Escherichia coli, and Chlamydia trachomatis D. The combination effect of compounds with antibiotics was examined by the minimum inhibitory concentration reduction assay. Their efflux pump (EP) inhibitory properties were assessed using real-time fluorimetry. Relative expressions of EP and quorum-sensing genes were studied by quantitative PCR. Results showed that a methylketone selenoester had remarkable antibacterial activity against Gram-positive bacteria and potentiated the activity of oxacillin in MRSA. Most of the selenocompounds showed significant anti-chlamydial effects. The selenoanhydride and the diselenodiester were active inhibitors of the AcrAB-TolC system. Based on these results it can be concluded that this group of selenocompounds can be attractive potential antibacterials and EP inhibitors. The discovery of new derivatives with a significant antibacterial activity as novel selenocompounds, is of high impact in the fight against resistant pathogens.

Download Full-text

Novel 2,4,6-Trimethylbenzenesulfonyl Hydrazones with Antibacterial Activity: Synthesis and In Vitro Study

Materials ◽

10.3390/ma14112723 ◽

2021 ◽

Vol 14 (11) ◽

pp. 2723

Author(s):

Łukasz Popiołek ◽

Sylwia Szeremeta ◽

Anna Biernasiuk ◽

Monika Wujec

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Potential Application ◽

Antimicrobial Agents ◽

Inhibitory Concentration ◽

Condensation Reaction ◽

In Vitro Study ◽

In Vitro Bioactivity ◽

Bacterial Strains

This research describes the synthesis and in vitro antimicrobial activity study of a series of 2,4,6-trimethylbenzenesulfonyl hydrazones. Twenty-five hydrazones (2–26) were synthesized on the basis of condensation reaction. The in vitro bioactivity study confirmed the potential application of obtained derivatives as antimicrobial agents. Among the tested compounds, the highest activity was discovered for derivative 24, which possessed minimal inhibitory concentration (MIC) ranging from 7.81 to 15.62 µg/mL against Gram-positive reference bacterial strains. Synthesized benzenesulfonyl hydrazones can be applied as potential ligands for the synthesis of bioactive metal complexes.

Download Full-text

Synthesis of Some Quinoxaline Sulfonamides as a Potential Antibacterial Agent

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i51b33521 ◽

2021 ◽

pp. 116-132

Author(s):

Festus O. Taiwo ◽

Craig A. Obafemi ◽

David A. Akinpelu A. Akinpelu

Keyword(s):

Antibacterial Activity ◽

Broad Spectrum ◽

Antibacterial Agent ◽

Antibacterial Property ◽

Antibacterial Properties ◽

Antimicrobial Properties ◽

Pharmacological Properties ◽

Antimicrobial Compounds ◽

Bacterial Strains ◽

Bactericidal Effects

Aims: This studies aims at the synthesis of new heterocyclic systems and study its biological and pharmacological properties. Objective: This study was designed to synthesized some quinoxaline-2,3-dione with sulfonamide moiety, characterize the synthesized compounds, and study the antimicrobial properties of the synthesized compounds on some bacterial strains. Materials and Methods: Six quinoxaline-6-sulfonohydrazone derivatives were synthesized by reacting quinoxaline-6-sulfonohydrazine with some substituted benzaldehydes and ketones. The compounds were tested for their potential antibacterial properties. Results: All the test compounds possessed promising antibacterial property against a panel of bacterial strains used for this study. The MIC values exhibited by these compounds ranged between 0.0313 and 0.250 mg/mL. Among the compounds tested, compound 2 showed appreciable antibacterial activity. Discussion and Conclusion: The study concluded that all the compounds exhibited appreciable bactericidal effects towards all the bacterial strains, particularly, compound 2 This is an indication that such compounds possessing broad spectrum activities will be useful in formulating antimicrobial compounds which could be used to treat infections caused by pathogens that are now developing resistance against the available antibiotics.

Download Full-text

BIOPROSPECTING FOR BACTERIAL ENDOPHYTES ASSOCIATED WITH ZINGIBERACEAE FAMILY RHIZOMES IN SIBOLANGIT FOREST, NORTH SUMATERA

International Journal of Science, Technology & Management ◽

10.46729/ijstm.v1i1.7 ◽

2020 ◽

Vol 1 (1) ◽

pp. 27-36

Author(s):

Jendri Mamangkey

Keyword(s):

Antibacterial Activity ◽

Protease Activity ◽

Antibacterial Agent ◽

Phosphate Solubilization ◽

Antibacterial Activities ◽

Chemical Diversity ◽

Bacterial Endophytes ◽

Bacterial Isolates ◽

Bacterial Strains ◽

Paper Disc

The present study was conducted aiming to isolate and characterize endophytic bacterial isolates with antibacterial ability, phosphate solubilization, and proteolytic activity from rhizomes of the Zingiberaceae family (Etlingera sp., Globba patens, Globba pendula, and Zingiber multibracteata). Nineteen bacterial isolates were obtained from Zingiberaceae rhizomes with isolate codes of EZS27, EZS18, EZS19, EZS25, EZS16, EZS08, EZS09, EZS13, EZS20, EZS14, EZS10, EZS11, EZS03, EZS05, EZS06, EZS43, EZS45, EZS47, and EZS28. The screening of the endophytes for antibacterial activity was done through the paper disc method. Four bacterial isolates presented antibacterial activities. EZS06 isolate inhibited the growth of EPEC (11 mm), P. vulgaris ATCC 13315 (10 mm), and L. monocytogenes BTCC B693 (9 mm). Also, EZS20 isolate inhibited the growth of S. aureus ATCC 29213 (17 mm), EZS28 isolate inhibited MRSA ATCC 43300 (8.6 mm), and EZS45 isolate inhibited S. Epidermidis ATCC 12228 (9 mm). The EZS19, EZS03, and EZS16 isolates dissolved the phosphate most effectively. Eight isolates (EZS19, EZS47, EZS27, EZS25, EZS09, EZS20, EZS45, and EZS06) showed the best protease activity. In general, our results showed that the endophytic bacterial strains can be used as a new and useful antibacterial agent since it showed antibacterial activity and chemical diversity. Furthermore, it also has the potential for exploitation in a wide variety of medical, agricultural, and industrial areas.

Download Full-text

Experimental and theoretical evaluation of two indol-steroid-cyclobuta-imidazole derivatives as antibacterial drugs

Biointerface Research in Applied Chemistry ◽

10.33263/briac95.405415 ◽

2019 ◽

Vol 9 (5) ◽

pp. 4405-4415 ◽

Cited By ~ 1

Keyword(s):

Amino Acids ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Minimum Inhibitory Concentration ◽

Antibacterial Agent ◽

Inhibitory Concentration ◽

Theoretical Data ◽

Novel Therapy ◽

Theoretical Evaluation ◽

Steroid Derivative

There some for treatment of infectios deseasses; however, some drugs can induce several adverse effects. The aim of this study was synthesizing and to determinate the antibacterial activity of two indol-steroid-cyclobuta-imidazole complex (compound 11 and 12) against Staphylococcus aureus, Escherichia colli and Klebsiella pneumoniae in a minimum inhibitory concentration (MIC) model, using gentamicin, ciprofloxacin, cefotaxime as controls. The following stage involved the theoretical evaluation of the interaction of both compounds 11 and 12 with the β-lactamase enzyme (5f1g) using a docking software. The data found indicate that compound 12 decrease the growth bacterial of Staphylococcus aureus, Escherichia colli and Streptococcus pneumoniae in comparison with the compound 11 and this effect only was similar to cefotaxime Other theoretical data indicated that compound 12 could interact with different type of amino acids residues such as Ser61, Leu116, Gln117, Asp120, Tyr147, Asn149, Ser209, Tyr218, Thr318, Asn342 involved in the surface of 5f1g compared with 11. These data indicate that; i) the steroid derivative (12) show better affinity by the 5f1g protein in comparison with compound 11 which is translated as higher antibacterial activity; ii) this compound is particularly interesting because could constitute a novel therapy as antibacterial agent.

Download Full-text

Biosynthesis of silver nanoparticles using Bacillus subitilis and its antibacterial activity

10.51429/ejmm30210 ◽

2021 ◽

Vol 30 (2) ◽

pp. 67-71

Author(s):

Ghada S.M. Abd el wahab

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Antibacterial Agent ◽

Antibacterial Activities ◽

Bacterial Strains ◽

Standard Strain ◽

Physical Chemical ◽

Inhibition Zones ◽

Reference Strains ◽

And Staphylococcus Aureus

Background: Silver nanoparticles (AgNPs) have recently been extensively investigated because of their superior physical, chemical, and biological characteristics, and their superiority is primarily due to the size, shape, composition, crystallinity, and structure of AgNPs compared to their bulk forms. Objective: The current study aimed to investigate the antibacterial activity of silver nanoparticles (AgNPs) synthesized intracellular by using standard strain Bacillus subitilis ATCC 6633 against reference strains Escherichia coli ATCC 2592 and Staphylococcus aureus ATCC 29737. Results: The synthesized AgNPs showed potent antibacterial activities against the two tested bacterial strains with inhibition zones ranged from 42 -52mm and MIC 27.2 µg / ml. The silver nanoparticles were characterized with particle size ≃ 100 nm and zeta potential -19. There was deformation in both tested strains upon treatment with AgNPs which was observed by Scanning Electron Microscopy (SEM). Conclusion: The results indicated that AgNPs could be used as an effective antibacterial agent.

Download Full-text