scholarly journals Strategies to Build Hybrid Protein–DNA Nanostructures

Nanomaterials ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1332
Author(s):  
Armando Hernandez-Garcia
Keyword(s):  
Dynamic Systems ◽  
Dna Nanotechnology ◽  
Chemical Properties ◽  
Hybrid Nanostructures ◽  
Advanced Materials ◽  
Dna Nanostructures ◽  
Hybrid Protein ◽  
Structural Protein ◽  
The Individual ◽  
Structural Dna Nanotechnology

Proteins and DNA exhibit key physical chemical properties that make them advantageous for building nanostructures with outstanding features. Both DNA and protein nanotechnology have growth notably and proved to be fertile disciplines. The combination of both types of nanotechnologies is helpful to overcome the individual weaknesses and limitations of each one, paving the way for the continuing diversification of structural nanotechnologies. Recent studies have implemented a synergistic combination of both biomolecules to assemble unique and sophisticate protein–DNA nanostructures. These hybrid nanostructures are highly programmable and display remarkable features that create new opportunities to build on the nanoscale. This review focuses on the strategies deployed to create hybrid protein–DNA nanostructures. Here, we discuss strategies such as polymerization, spatial directing and organizing, coating, and rigidizing or folding DNA into particular shapes or moving parts. The enrichment of structural DNA nanotechnology by incorporating protein nanotechnology has been clearly demonstrated and still shows a large potential to create useful and advanced materials with cell-like properties or dynamic systems. It can be expected that structural protein–DNA nanotechnology will open new avenues in the fabrication of nanoassemblies with unique functional applications and enrich the toolbox of bionanotechnology.

scholarly journals Strategies to Build Hybrid Protein-DNA Nanostructures

2021 ◽  
Author(s):  
Armando Hernandez-Garcia
Keyword(s):  
Dynamic Systems ◽  
Dna Nanotechnology ◽  
Chemical Properties ◽  
Hybrid Nanostructures ◽  
Advanced Materials ◽  
Dna Nanostructures ◽  
Hybrid Protein ◽  
Structural Protein ◽  
The Individual ◽  
Structural Dna Nanotechnology

Proteins and DNA exhibit key physical chemical properties that make them advantageous for building nanostructures with outstanding features. Both DNA and protein nanotechnology have growth notably and proved to be fertile disciplines. The combination of both types of nanotechnologies is helpful to overcome the individual weaknesses and limitations of each one, paving the way for the continuing diversification of the structural nanotechnologies. Recent studies have implemented a synergistic combination of both biomolecules to assemble unique and sophisticate protein-DNA nanostructures. These hybrid nanostructures are highly programmable and display remarkable features that create new opportunities to build in the nanoscale. This review focuses on the strategies deployed to create hybrid protein-DNA nanostructures. Here, we will discuss strategies such as polymerization, spatial directing and organizing, coating, rigidizing or folding DNA into particular shapes or moving parts. The enrichment of structural DNA nanotechnology by incorporating protein nanotechnology has been clearly demonstrated and still shows a large potential to create useful and advanced materials with cell-like properties or dynamic systems. It can be expected that structural protein-DNA nanotechnology will open new avenues in the fabrication of nano-assemblies with unique functional applications and enrich the toolbox of bionanotechnology.

scholarly journals Hybrid Nanoassemblies from Viruses and DNA Nanostructures

Nanomaterials ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1413
Author(s):  
Sofia Ojasalo ◽  
Petteri Piskunen ◽  
Boxuan Shen ◽  
Mauri A. Kostiainen ◽  
Veikko Linko
Keyword(s):  
Cell Activation ◽  
Immune Cell ◽  
Dna Nanotechnology ◽  
Biological Properties ◽  
Dna Nanostructures ◽  
Dna Structures ◽  
Nanoscale Structures ◽  
Immune Cell Activation ◽  
Wide Range ◽  
Structural Dna Nanotechnology

Viruses are among the most intriguing nanostructures found in nature. Their atomically precise shapes and unique biological properties, especially in protecting and transferring genetic information, have enabled a plethora of biomedical applications. On the other hand, structural DNA nanotechnology has recently emerged as a highly useful tool to create programmable nanoscale structures. They can be extended to user defined devices to exhibit a wide range of static, as well as dynamic functions. In this review, we feature the recent development of virus-DNA hybrid materials. Such structures exhibit the best features of both worlds by combining the biological properties of viruses with the highly controlled assembly properties of DNA. We present how the DNA shapes can act as “structured” genomic material and direct the formation of virus capsid proteins or be encapsulated inside symmetrical capsids. Tobacco mosaic virus-DNA hybrids are discussed as the examples of dynamic systems and directed formation of conjugates. Finally, we highlight virus-mimicking approaches based on lipid- and protein-coated DNA structures that may elicit enhanced stability, immunocompatibility and delivery properties. This development also paves the way for DNA-based vaccines as the programmable nano-objects can be used for controlling immune cell activation.

scholarly journals 3D DNA Nanostructures: The Nanoscale Architect

2021 ◽  
Vol 11 (6) ◽  
pp. 2624
Author(s):  
Daniel Fu ◽  
John Reif
Keyword(s):  
Single Molecule ◽  
Mechanical Design ◽  
Dna Nanotechnology ◽  
Dna Nanostructures ◽  
Biophysical Studies ◽  
Significant Research ◽  
Core Concepts ◽  
Potential Applications ◽  
The Stability ◽  
Structural Dna Nanotechnology

Structural DNA nanotechnology is a pioneering biotechnology that presents the opportunity to engineer DNA-based hardware that will mediate a profound interface to the nanoscale. To date, an enormous library of shaped 3D DNA nanostructures have been designed and assembled. Moreover, recent research has demonstrated DNA nanostructures that are not only static but can exhibit specific dynamic motion. DNA nanostructures have thus garnered significant research interest as a template for pursuing shape and motion-dependent nanoscale phenomena. Potential applications have been explored in many interdisciplinary areas spanning medicine, biosensing, nanofabrication, plasmonics, single-molecule chemistry, and facilitating biophysical studies. In this review, we begin with a brief overview of general and versatile design techniques for 3D DNA nanostructures as well as some techniques and studies that have focused on improving the stability of DNA nanostructures in diverse environments, which is pivotal for its reliable utilization in downstream applications. Our main focus will be to compile a wide body of existing research on applications of 3D DNA nanostructures that demonstrably rely on the versatility of their mechanical design. Furthermore, we frame reviewed applications into three primary categories, namely encapsulation, surface templating, and nanomechanics, that we propose to be archetypal shape- or motion-related functions of DNA nanostructures found in nanoscience applications. Our intent is to identify core concepts that may define and motivate specific directions of progress in this field as we conclude the review with some perspectives on the future.

scholarly journals Fabrication of Graphene/Molybdenum Disulfide Composites and Their Usage as Actuators for Electrochemical Sensors and Biosensors

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3374 ◽  
Author(s):  
Jiri Kudr ◽  
Vojtech Adam ◽  
Ondrej Zitka
Keyword(s):  
Electrochemical Sensors ◽  
Transition Metal Dichalcogenides ◽  
Chemical Properties ◽  
Hybrid Nanostructures ◽  
Advanced Materials ◽  
Materials Synthesis ◽  
Comprehensive Overview ◽  
Electroanalytical Chemistry ◽  
Functional Composites ◽  
Metal Dichalcogenides

From the rediscovery of graphene in 2004, the interest in layered graphene analogs has been exponentially growing through various fields of science. Due to their unique properties, novel two-dimensional family of materials and especially transition metal dichalcogenides are promising for development of advanced materials of unprecedented functions. Progress in 2D materials synthesis paved the way for the studies on their hybridization with other materials to create functional composites, whose electronic, physical or chemical properties can be engineered for special applications. In this review we focused on recent progress in graphene-based and MoS2 hybrid nanostructures. We summarized and discussed various fabrication approaches and mentioned different 2D and 3D structures of composite materials with emphasis on their advances for electroanalytical chemistry. The major part of this review provides a comprehensive overview of the application of graphene-based materials and MoS2 composites in the fields of electrochemical sensors and biosensors.

ARTIFICIALLY DESIGNED DNA NANOSTRUCTURES

NANO ◽  
2009 ◽  
Vol 04 (03) ◽  
pp. 119-139 ◽  
Author(s):  
RASHID AMIN ◽  
SOYEON KIM ◽  
SUNG HA PARK ◽  
THOMAS HENRY LABEAN
Keyword(s):  
Dna Sequences ◽  
Self Assembly ◽  
Dna Nanotechnology ◽  
Medical Applications ◽  
Dna Nanostructures ◽  
Base Pairing ◽  
Assembly Instructions ◽  
Artificial Dna ◽  
Structural Dna Nanotechnology ◽  
Complex Nanostructures

In the field of structural DNA nanotechnology, researchers create artificial DNA sequences to self-assemble into target molecular superstructures and nanostructures. The well-understood Watson–Crick base-pairing rules are used to encode assembly instructions directly into the DNA molecules. A wide variety of complex nanostructures has been created using this method. DNA directed self-assembly is now being adapted for use in the nanofabrication of functional structures for use in electronics, photonics, and medical applications.

Single-molecule methods in structural DNA nanotechnology

2020 ◽  
Vol 49 (13) ◽  
pp. 4220-4233 ◽  
Author(s):  
Casey M. Platnich ◽  
Felix J. Rizzuto ◽  
Gonzalo Cosa ◽  
Hanadi F. Sleiman
Keyword(s):  
Single Molecule ◽  
Method Development ◽  
Dna Nanotechnology ◽  
Dna Nanostructures ◽  
Structural Dna Nanotechnology ◽  
The Relationship

In this tutorial review, we explore the suite of single-molecule techniques currently available to probe DNA nanostructures and highlight the relationship between single-molecule method development and DNA nanotechology.

Structural DNA nanotechnology: Immobile Holliday junctions to artificial robots

2022 ◽  
Vol 22 ◽  
Author(s):  
Raghu Pradeep Narayanan ◽  
Leeza Abraham
Keyword(s):  
Dna Nanotechnology ◽  
Building Blocks ◽  
Holliday Junctions ◽  
Dna Origami ◽  
Building Structures ◽  
Dna Nanostructures ◽  
3 Dimensional ◽  
Scientific World ◽  
2D And 3D ◽  
Structural Dna Nanotechnology

Abstreact: DNA nanotechnology marvels the scientific world with its capabilities to design, engineer, and demonstrate nanoscale shapes. This review is a condensed version walking the reader through the structural developments in the field over the past 40 years starting from the basic design rules of the double-stranded building block to the most recent advancements in self-assembled hierarchically achieved structures to date. It builds off from the fundamental motivation of building 3-dimensional (3D) lattice structures of tunable cavities going all the way up to artificial nanorobots fighting cancer. The review starts by covering the most important developments from the fundamental bottom-up approach of building structures, which is the ‘tile’ based approach covering 1D, 2D, and 3D building blocks, after which, the top-down approach using DNA origami and DNA bricks is also covered. Thereafter, DNA nanostructures assembled using not so commonly used (yet promising) techniques like i-motifs, quadruplexes, and kissing loops are covered. Highlights from the field of dynamic DNA nanostructures have been covered as well, walking the reader through the various approaches used within the field to achieve movement. The article finally concludes by giving the authors a view of what the future of the field might look like while suggesting in parallel new directions that fellow/future DNA nanotechnologists could think about.

Structural DNA Nanotechnology: Artificial Nanostructures for Biomedical Research

2018 ◽  
Vol 20 (1) ◽  
pp. 375-401 ◽  
Author(s):  
Yonggang Ke ◽  
Carlos Castro ◽  
Jong Hyun Choi
Keyword(s):  
Biomedical Research ◽  
Self Assembly ◽  
Biomedical Applications ◽  
Dna Nanotechnology ◽  
Dna Nanostructures ◽  
Considerable Effort ◽  
Artificial Nanostructures ◽  
Active Pursuit ◽  
External Stimuli ◽  
Structural Dna Nanotechnology

Structural DNA nanotechnology utilizes synthetic or biologic DNA as designer molecules for the self-assembly of artificial nanostructures. The field is founded upon the specific interactions between DNA molecules, known as Watson–Crick base pairing. After decades of active pursuit, DNA has demonstrated unprecedented versatility in constructing artificial nanostructures with significant complexity and programmability. The nanostructures could be either static, with well-controlled physicochemical properties, or dynamic, with the ability to reconfigure upon external stimuli. Researchers have devoted considerable effort to exploring the usability of DNA nanostructures in biomedical research. We review the basic design methods for fabricating both static and dynamic DNA nanostructures, along with their biomedical applications in fields such as biosensing, bioimaging, and drug delivery.

scholarly journals Generating DNA Origami Nanostructures through Shape Annealing

2021 ◽  
Vol 11 (7) ◽  
pp. 2950
Author(s):  
Bolutito Babatunde ◽  
D. Sebastian Arias ◽  
Jonathan Cagan ◽  
Rebecca E. Taylor
Keyword(s):  
Self Assembly ◽  
Design Space ◽  
Simulated Annealing Algorithm ◽  
Dna Nanotechnology ◽  
Dna Origami ◽  
Design Tools ◽  
Dna Nanostructures ◽  
Formal Approach ◽  
Annealing Algorithm ◽  
Structural Dna Nanotechnology

Structural DNA nanotechnology involves the design and self-assembly of DNA-based nanostructures. As a field, it has progressed at an exponential rate over recent years. The demand for unique DNA origami nanostructures has driven the development of design tools, but current CAD tools for structural DNA nanotechnology are limited by requiring users to fully conceptualize a design for implementation. This article introduces a novel formal approach for routing the single-stranded scaffold DNA that defines the shape of DNA origami nanostructures. This approach for automated scaffold routing broadens the design space and generates complex multilayer DNA origami designs in an optimally driven way, based on a set of constraints and desired features. This technique computes unique designs of DNA origami assemblies by utilizing shape annealing, which is an integration of shape grammars and the simulated annealing algorithm. The results presented in this article illustrate the potential of the technique to code desired features into DNA nanostructures.

Chlorinated Organics in Nearshore Waters and Tributaries of the St. Clair River

1986 ◽  
Vol 21 (3) ◽  
pp. 344-350 ◽  
Author(s):  
Barry G. Oliver ◽  
Klaus L.E. Kaiser
Keyword(s):  
United States ◽  
Suspended Sediment ◽  
Waste Disposal ◽  
Large Volume ◽  
Water Samples ◽  
Chemical Properties ◽  
Low Level ◽  
Chemical Company ◽  
Physical Chemical ◽  
The Individual

Abstract The concent rat ions of hexachloroethane (HCE), hexachlorobutadiene (HCBD), pentachlorobenzene (QCB), hexachlorobenzene (HCB) and octachlorostyrene (OCS) in large volume water samples show that the major sources of these chemicals to the St. Clair River are Dow Chemical Company effluents and, to a lesser degree, Sarnia’s Township ditch which drains one of Dow’s waste disposal sites. Tributaries entering the river on both sides of the Canada/United States border contain measurable concentrations of these chemicals indicating low level contamination throughout the area. The degree of water/suspended sediment partitioning of the chemicals (Kp) was studied. Kp values for the individual chemicals changed in a manner consistent with changes in their physical-chemical properties.

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