Current Knowledge of Silver and Gold Nanoparticles in Laboratory Research—Application, Toxicity, Cellular Uptake

Silver and gold nanoparticles can be found in a range of household products related to almost every area of life, including patches, bandages, paints, sportswear, personal care products, food storage equipment, cosmetics, disinfectants, etc. Their confirmed ability to enter the organism through respiratory and digestive systems, skin, and crossing the blood–brain barrier raises questions of their potential effect on cell function. Therefore, this manuscript aimed to summarize recent reports concerning the influence of variables such as size, shape, concentration, type of coating, or incubation time, on effects of gold and silver nanoparticles on cultured cell lines. Due to the increasingly common use of AgNP and AuNP in multiple branches of the industry, further studies on the effects of nanoparticles on different types of cells and the general natural environment are needed to enable their long-term use. However, some environmentally friendly solutions to chemically synthesized nanoparticles are also investigated, such as plant-based synthesis methods.

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Green ultrasonic synthesis, Characterization and Antibacterial activity of Silver and Gold Nanoparticles mediated by Ganoderma lucidum extract

Advances in Materials Science and Engineering ◽

10.33140/jamser.04.02.02 ◽

2020 ◽

Vol 4 (2) ◽

Keyword(s):

Gold Nanoparticles ◽

Silver Nanoparticles ◽

Antibacterial Activity ◽

Metal Nanoparticles ◽

Cytotoxic Activities ◽

Gold And Silver Nanoparticles ◽

Silver And Gold Nanoparticles ◽

E Coli ◽

Inhibition Zones

Metal nanoparticles possess an extensive scientific and technological significance due to their unique physiochemical properties and their potential applications in different fields like medicine. Silver and gold nanoparticles have shown to have antibacterial and cytotoxic activities. Conventional methods used in the synthesis of the metal nanoparticles involve use of toxic chemicals making them unsuitable for use in medical field. In our continued effort to explore for simple and eco-friendly methods to synthesize the metal nanoparticles, we here describe synthesis and characterization of gold and silver nanoparticles using Gonaderma lucidum, wild non-edible medicinal mushroom. G. lucidum mushroom contain bioactive compounds which can be involved in the reduction, capping and stabilization of the nanoparticles. Antibacterial activity analysis was done on E. coli and S. aureus. The synthesis was done on ultrasonic bath. Characterization of the metal nanoparticles was done by UV-VIS., High Resolution Transmission Electron Microscope (HRTEM) and FTIR. HRTEM analysis showed that both silver and gold nanoparticles were spherical in shape with an average size of 15.82±3.69 nm for silver and 24.73±5.124nm for gold nanoparticles (AuNPs). FTIR analysis showed OH and -C=C- stretching vibrations, an indication of presence of functional groups of biomolecules capping both gold and silver nanoparticles. AgNPs showed inhibition zones of 15.5±0.09mm and 13.3±0.14mm while AuNPs had inhibition zones of 14.510±0.35 and 13.3±0.50mm on E. coli and S. aureus respectively. The findings indicate the potential use of AgNPs and AuNPs in development of drugs in management of pathogenic bacteria.

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Biomedical Applications of Silver and Gold Nanoparticles: Effective and Safe Non-Viral Delivery Vehicles

Journal of Applied Biotechnology & Bioengineering ◽

10.15406/jabb.2017.03.00059 ◽

2017 ◽

Vol 3 (2) ◽

Cited By ~ 4

Author(s):

Kirti Rani

Keyword(s):

Gold Nanoparticles ◽

Biomedical Applications ◽

Silver And Gold Nanoparticles ◽

Delivery Vehicles

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Antibacterial and Anti-Biofilm Biosynthesised Silver and Gold Nanoparticles for Medical Applications: Mechanism of Action, Toxicity and Current Status

Current Drug Delivery ◽

10.2174/1567201817666191227094334 ◽

2020 ◽

Vol 17 (2) ◽

pp. 88-100 ◽

Cited By ~ 8

Author(s):

Sundos Suleman Ismail Abdalla ◽

Haliza Katas ◽

Fazren Azmi ◽

Mohd Fauzi Mh Busra

Keyword(s):

Gold Nanoparticles ◽

Synthesis Method ◽

Chemical Characterization ◽

Current Status ◽

Silver And Gold Nanoparticles ◽

Physical And Chemical Characterization ◽

Biosynthesized Agnps ◽

Physical And Chemical ◽

Insight Into ◽

Bio Synthesis

Fast progress in nanoscience and nanotechnology has contributed to the way in which people diagnose, combat, and overcome various diseases differently from the conventional methods. Metal nanoparticles, mainly silver and gold nanoparticles (AgNPs and AuNPs, respectively), are currently developed for many applications in the medical and pharmaceutical area including as antibacterial, antibiofilm as well as anti-leshmanial agents, drug delivery systems, diagnostics tools, as well as being included in personal care products and cosmetics. In this review, the preparation of AgNPs and AuNPs using different methods is discussed, particularly the green or bio- synthesis method as well as common methods used for their physical and chemical characterization. In addition, the mechanisms of the antimicrobial and anti-biofilm activity of AgNPs and AuNPs are discussed, along with the toxicity of both nanoparticles. The review will provide insight into the potential of biosynthesized AgNPs and AuNPs as antimicrobial nanomaterial agents for future use.

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Stabilization of Silver and Gold Nanoparticles: Preservation and Improvement of Plasmonic Functionalities

Chemical Reviews ◽

10.1021/acs.chemrev.8b00341 ◽

2018 ◽

Vol 119 (1) ◽

pp. 664-699 ◽

Cited By ~ 107

Author(s):

Hyunho Kang ◽

Joseph T. Buchman ◽

Rebeca S. Rodriguez ◽

Hattie L. Ring ◽

Jiayi He ◽

...

Keyword(s):

Gold Nanoparticles ◽

Silver And Gold Nanoparticles

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Isolation and Optimization of Culture Conditions of Thraustochytrium kinnei for Biomass Production, Nanoparticle Synthesis, Antioxidant and Antimicrobial Activities

Journal of Marine Science and Engineering ◽

10.3390/jmse9060678 ◽

2021 ◽

Vol 9 (6) ◽

pp. 678

Author(s):

Kaliyamoorthy Kalidasan ◽

Nabikhan Asmathunisha ◽

Venugopal Gomathi ◽

Laurent Dufossé ◽

Kandasamy Kathiresan

Keyword(s):

Antimicrobial Activity ◽

Gold Nanoparticles ◽

Silver Nanoparticles ◽

Biomass Production ◽

Petroleum Ether Extract ◽

Antimicrobial Activities ◽

Culture Conditions ◽

Gold And Silver Nanoparticles ◽

Optimization Of Culture Conditions ◽

Antioxidant And Antimicrobial Activities

This work deals with the identification of a predominant thraustochytrid strain, the optimization of culture conditions, the synthesis of nanoparticles, and the evaluation of antioxidant and antimicrobial activities in biomass extracts and nanoparticles. Thraustochytrium kinnei was identified as a predominant strain from decomposing mangrove leaves, and its culture conditions were optimized for maximum biomass production of 13.53 g·L−1, with total lipids of 41.33% and DHA of 39.16% of total fatty acids. Furthermore, the strain was shown to synthesize gold and silver nanoparticles in the size ranges of 10–85 nm and 5–90 nm, respectively. Silver nanoparticles exhibited higher total antioxidant and DPPH activities than gold nanoparticles and methanol extract of the strain. The silver nanoparticles showed higher antimicrobial activity than gold nanoparticles and petroleum ether extract of the strain. Thus, Thraustochytrium kinnei is proven to be promising for synthesis of silver nanoparticles with high antioxidant and antimicrobial activity.

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Interaction of Differently Sized, Shaped, and Functionalized Silver and Gold Nanoparticles with Glycosylated versus Nonglycosylated Transferrin

ACS Applied Materials & Interfaces ◽

10.1021/acsami.1c04063 ◽

2021 ◽

Author(s):

Rinea Barbir ◽

Rafael Ramírez Jiménez ◽

Rafael Martín-Rapún ◽

Vida Strasser ◽

Darija Domazet Jurašin ◽

...

Keyword(s):

Gold Nanoparticles ◽

Silver And Gold Nanoparticles

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Current Strategies for Noble Metal Nanoparticle Synthesis

Nanoscale Research Letters ◽

10.1186/s11671-021-03480-8 ◽

2021 ◽

Vol 16 (1) ◽

Author(s):

Giyaullah Habibullah ◽

Jitka Viktorova ◽

Tomas Ruml

Keyword(s):

Noble Metal ◽

Noble Metals ◽

Drug Carriers ◽

Industrial Applications ◽

Metal Nanoparticle ◽

Noble Metal Nanoparticles ◽

Synthesis Methods ◽

Gold And Silver Nanoparticles ◽

Diverse Range ◽

The Past

AbstractNoble metals have played an integral part in human history for centuries; however, their integration with recent advances in nanotechnology and material sciences have provided new research opportunities in both academia and industry, which has resulted in a new array of advanced applications, including medical ones. Noble metal nanoparticles (NMNPs) have been of great importance in the field of biomedicine over the past few decades due to their importance in personalized healthcare and diagnostics. In particular, platinum, gold and silver nanoparticles have achieved the most dominant spot in the list, thanks to a very diverse range of industrial applications, including biomedical ones such as antimicrobial and antiviral agents, diagnostics, drug carriers and imaging probes. In particular, their superior resistance to extreme conditions of corrosion and oxidation is highly appreciated. Notably, in the past two decades there has been a tremendous advancement in the development of new strategies of more cost-effective and robust NMNP synthesis methods that provide materials with highly tunable physicochemical, optical and thermal properties, and biochemical functionalities. As a result, new advanced hybrid NMNPs with polymer, graphene, carbon nanotubes, quantum dots and core–shell systems have been developed with even more enhanced physicochemical characteristics that has led to exceptional diagnostic and therapeutic applications. In this review, we aim to summarize current advances in the synthesis of NMNPs (Au, Ag and Pt).

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Green Silver and Gold Nanoparticles: Biological Synthesis Approaches and Potentials for Biomedical Applications

Molecules ◽

10.3390/molecules26040844 ◽

2021 ◽

Vol 26 (4) ◽

pp. 844 ◽

Cited By ~ 2

Author(s):

Andrea Rónavári ◽

Nóra Igaz ◽

Dóra I. Adamecz ◽

Bettina Szerencsés ◽

Csaba Molnar ◽

...

Keyword(s):

Gold Nanoparticles ◽

Biomedical Applications ◽

Biological Activities ◽

Large Body ◽

Chemical Properties ◽

Biological Synthesis ◽

Silver And Gold Nanoparticles ◽

Comprehensive Collection ◽

Synthetic Approaches ◽

Physical And Chemical

The nanomaterial industry generates gigantic quantities of metal-based nanomaterials for various technological and biomedical applications; however, concomitantly, it places a massive burden on the environment by utilizing toxic chemicals for the production process and leaving hazardous waste materials behind. Moreover, the employed, often unpleasant chemicals can affect the biocompatibility of the generated particles and severely restrict their application possibilities. On these grounds, green synthetic approaches have emerged, offering eco-friendly, sustainable, nature-derived alternative production methods, thus attenuating the ecological footprint of the nanomaterial industry. In the last decade, a plethora of biological materials has been tested to probe their suitability for nanomaterial synthesis. Although most of these approaches were successful, a large body of evidence indicates that the green material or entity used for the production would substantially define the physical and chemical properties and as a consequence, the biological activities of the obtained nanomaterials. The present review provides a comprehensive collection of the most recent green methodologies, surveys the major nanoparticle characterization techniques and screens the effects triggered by the obtained nanomaterials in various living systems to give an impression on the biomedical potential of green synthesized silver and gold nanoparticles.

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Size and Shape-Dependent Antimicrobial Activities of Silver and Gold Nanoparticles: A Model Study as Potential Fungicides

Molecules ◽

10.3390/molecules25112682 ◽

2020 ◽

Vol 25 (11) ◽

pp. 2682 ◽

Cited By ~ 2

Author(s):

Francis J. Osonga ◽

Ali Akgul ◽

Idris Yazgan ◽

Ayfer Akgul ◽

Gaddi B. Eshun ◽

...

Keyword(s):

Gold Nanoparticles ◽

Pathogenic Fungi ◽

Antimicrobial Activities ◽

Plant Diseases ◽

Production Plant ◽

Silver And Gold Nanoparticles ◽

Size And Shape ◽

Model Study ◽

Pathogenic Microbes ◽

Bacteria And Fungi

Plant-based pathogenic microbes hinder the yield and quality of food production. Plant diseases have caused an increase in food costs due to crop destruction. There is a need to develop novel methods that can target and mitigate pathogenic microbes. This study focuses on investigating the effects of luteolin tetraphosphate derived silver nanoparticles (LTP-AgNPs) and gold nanoparticles (LTP-AuNPs) as a therapeutic agent on the growth and expression of plant-based bacteria and fungi. In this study, the silver and gold nanoparticles were synthesized at room temperature using luteolin tetraphosphate (LTP) as the reducing and capping agents. The synthesis of LTP-AgNPs and LTP-AuNP was characterized by Transmission Electron Microscopy (TEM) and size distribution. The TEM images of both LTP-AgNPs and LTP-AuNPs showed different sizes and shapes (spherical, quasi-spherical, and cuboidal). The antimicrobial test was conducted using fungi: Aspergillus nidulans, Trichaptum biforme, Penicillium italicum, Fusarium oxysporum, and Colletotrichum gloeosporioides, while the class of bacteria employed include Pseudomonas aeruginosa, Aeromonas hydrophila, Escherichia coli, and Citrobacter freundii as Gram (−) bacteria, and Listeria monocytogenes and Staphylococcus epidermidis as Gram (+) bacterium. The antifungal study demonstrated the selective size and shape-dependent capabilities in which smaller sized spherical (9 nm) and quasi-spherical (21 nm) AgNPs exhibited 100% inhibition of the tested fungi and bacteria. The LTP-AgNPs exhibited a higher antimicrobial activity than LTP-AuNPs. We have demonstrated that smaller sized AgNPs showed excellent inhibition of A. nidulans growth compared to the larger size nanoparticles. These results suggest that LTP-AuNP and LTP-AgNPs could be used to address the detection and remediation of pathogenic fungi, respectively.

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