scholarly journals Physicochemical Properties and Effects of Honeys on Key Biomarkers of Oxidative Stress and Cholesterol Homeostasis in HepG2 Cells

Nutrients ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 151
Author(s):  
Huong Thi Lan Nguyen ◽  
Stefan Kasapis ◽  
Nitin Mantri
Keyword(s):  
Oxidative Stress ◽  
Hepg2 Cells ◽  
Antioxidant Activities ◽  
Biochemical Properties ◽  
Cholesterol Homeostasis ◽  
Protective Effects ◽  
Cholesterol Reduction ◽  
Phenolic Contents ◽  
Biomarkers Of Oxidative Stress ◽  
Newtonian Behavior

Manuka honey and newly developed honeys (arjuna, guggul, jiaogulan and olive) were examined for their physicochemical, biochemical properties and effects on oxidative stress and cholesterol homeostasis in fatty acid-induced HepG2 cells. The honeys exhibited standard moisture content (<20%), electrical conductivity (<0.8 mS/cm), acidic pH, and monosaccharides (>60%), except olive honey (<60% total monosaccharides). They all expressed non-Newtonian behavior and 05 typical regions of the FTIR spectra as those of natural ones. Guggul and arjuna, manuka honeys showed the highest phenolic contents, correlating with their significant antioxidant activities. Arjuna, guggul and manuka honeys demonstrated the agreement of total cholesterol reduction and the transcriptional levels of AMPK, SREBP2, HCMGR, LDLR, LXRα. Jiaogulan honey showed the least antioxidant content and activity, but it was the most cytotoxic. Both jiaogulan and olive honeys modulated the tested gene in the pattern that should lead to a lower TC content, but this reduction did not occur after 24 h. All 2% concentrations of tested honeys elicited a clearer effect on NQO1 gene expression. In conclusion, the new honeys complied with international norms for natural honeys and we provide partial evidence for the protective effects of manuka, arjuna and guggul honeys amongst the tested ones on key biomarkers of oxidative stress and cholesterol homeostasis, pending further studies to better understand their modes of action.

scholarly journals Activity Guided Isolation of Phenolic Compositions from Anneslea fragrans Wall. and Their Cytoprotective Effect against Hydrogen Peroxide Induced Oxidative Stress in HepG2 Cells

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3690
Author(s):  
Shuyue He ◽  
Xiaoyan Cui ◽  
Afsar Khan ◽  
Yaping Liu ◽  
Yudan Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Hepg2 Cells ◽  
Antioxidant Activities ◽  
Folk Medicine ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Protective Effects ◽  
Significant Difference ◽  
Nonpolar Organic

Anneslea fragrans Wall., commonly known as “Pangpo Tea”, is traditionally used as a folk medicine and healthy tea for the treatment of liver and intestine diseases. The aim of this study was to purify the antioxidative and cytoprotective polyphenols from A. fragrans leaves. After fractionation with polar and nonpolar organic solvents, the fractions of aqueous ethanol extract were evaluated for their total phenolic (TPC) and flavonoid contents (TFC) and antioxidant activities (DPPH, ABTS, and FRAP assays). The n-butanol fraction (BF) showed the highest TPC and TFC with the strongest antioxidant activity. The bio-guided chromatography of BF led to the purification of six flavonoids (1–6) and one benzoquinolethanoid (7). The structures of these compounds were determined by NMR and MS techniques. Compound 6 had the strongest antioxidant capacity, which was followed by 5 and 2. The protective effect of the isolated compounds on hydrogen peroxide (H2O2)-induced oxidative stress in HepG2 cells revealed that the compounds 5 and 6 exhibited better protective effects by inhibiting ROS productions, having no significant difference with vitamin C (p > 0.05), whereas 6 showed the best anti-apoptosis activity. The results suggest that A. fragrans could serve as a valuable antioxidant phytochemical source for developing functional food and health nutraceutical products.

The protective effects of carvacrol and thymol against paracetamol–induced toxicity on human hepatocellular carcinoma cell lines (HepG2)

2016 ◽  
Vol 35 (12) ◽  
pp. 1252-1263 ◽  
Author(s):  
SS Palabiyik ◽  
E Karakus ◽  
Z Halici ◽  
E Cadirci ◽  
Y Bayir ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Inflammatory Cytokines ◽  
Hepg2 Cells ◽  
Folk Medicine ◽  
Hepatoprotective Activity ◽  
High Dose ◽  
Protective Effects ◽  
And Oxidative Stress ◽  
Pro Inflammatory Cytokines

Acetaminophen (APAP) overdose could induce liver damage and lead to acute liver failure. The treatment of APAP overdoses could be improved by new therapeutic strategies. Thymus spp., which has many beneficial effects and has been used in folk medicine, is one such potential strategy. In the present study, the hepatoprotective activity of the main constituents of Thymus spp., carvacrol and thymol, were evaluated in light of APAP-induced hepatotoxicity. We hoped to understand the hepatoprotective mechanism of these agents on the antioxidant system and pro-inflammatory cytokines in vitro. Dose-dependent effects of thymol and carvacrol (25, 50, and 100 µM) were tested on cultured HepG2 cells. N-Acetylcysteine (NAC) was tested as positive control. We showed that APAP inhibited HepG2 cell growth by inducing inflammation and oxidative stress. Incubating APAP-exposed HepG2 cells with carvacrol and thymol for 24 h ameliorated this inflammation and oxidative stress. We also evaluated alanine transaminase and lactate dehydrogenase levels of HepG2 cells. We found that thymol and carvacrol protected against APAP-induced toxicity in HepG2 cells by increasing antioxidant activity and reducing pro-inflammatory cytokines, such as tumor necrosis factor α and interleukin 1β. Taking together high-dose thymol and carvacrol treatment has an effect close to NAC treatment in APAP toxicity, but thymol has better treatment effect than carvacrol.

scholarly journals A Polysaccharide Purified from Morchella conica Pers. Prevents Oxidative Stress Induced by H2O2 in Human Embryonic Kidney (HEK) 293T Cells

2018 ◽  
Vol 19 (12) ◽  
pp. 4027 ◽  
Author(s):  
Na Xu ◽  
Yi Lu ◽  
Jumin Hou ◽  
Chao Liu ◽  
Yonghai Sun
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activities ◽  
Average Molecular Weight ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ultrastructural Observation ◽  
Protective Effects ◽  
Ferrous Ions ◽  
Nuclear Condensation ◽  
Morchella Conica

Morchella conica Pers. (M. conica) has been used both as a medical and edible mushroom and possesses antimicrobial properties and antioxidant activities. However, the antioxidant properties of polysaccharides purified from M. conica have not been studied. The aim of this study was to investigate the in vitro antioxidant properties of a polysaccharide NMCP-2 (neutral M. conica polysaccharides-2) purified from M. conica, as determined by radical scavenging assay and H2O2-induced oxidative stress in HEK 293T cells. Results showed that NMCP-2 with an average molecular weight of 48.3 kDa possessed a much stronger chelating ability on ferrous ions and a higher ability to scavenge radical scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) than the other purified fraction of NMCP-1 from M. conica. Moreover, 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetra-zolium bromide (MTT) assay showed that NMCP-2 dose-dependently preserved cell viability of H2O2-induced cells. The NMCP-2 pretreated group reduced the generation of reactive oxygen species (ROS) content and increased the mitochondria membrane potential (MMP) levels. In addition, Hoechst 33342 staining revealed cells treated with NMCP-2 declined nuclear condensation. Ultrastructural observation revealed that NMCP-2 pretreatment alleviated the ruptured mitochondria when exposed to H2O2. Furthermore, western blot analysis showed that NMCP-2 prevented significant downregulation of the protein expression of Bax, cleaved caspases 3, and upregulated Bcl-2 levels. These results suggest the protective effects of NMCP-2 against H2O2-induced injury in HEK 293T cells. NMCP-2 could be used as a natural antioxidant of functional foods and natural drugs.

scholarly journals Protective effects of resveratrol on biomarkers of oxidative stress, biochemical and histopathological changes induced by sub-chronic oral glyphosate-based herbicide in rats

2019 ◽  
Vol 8 (2) ◽  
pp. 238-245 ◽  
Author(s):  
Ruhi Turkmen ◽  
Yavuz Osman Birdane ◽  
Hasan Huseyin Demirel ◽  
Mustafa Kabu ◽  
Sinan Ince
Keyword(s):  
Oxidative Stress ◽  
Chronic Toxicity ◽  
Toxicity Study ◽  
Protective Effects ◽  
Histopathological Changes ◽  
Blood Brain ◽  
Biomarkers Of Oxidative Stress

The aim of this sub-chronic toxicity study is to determine the protective effects of Resveratrol (Res) on oxidative stress, biochemical and histopathological changes induced by glyphosate-based herbicide (GBH) in the blood, brain, heart, liver and renal tissues.

scholarly journals 57 Carnosine prevents oxidative damage in myoblast cells derived from porcine skeletal muscle

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 59-59
Author(s):  
Marie-France Palin ◽  
Jérôme Lapointe ◽  
Claude Gariépy ◽  
Danièle Beaudry ◽  
Claudia Kalbe
Keyword(s):  
Oxidative Stress ◽  
Oxidative Damage ◽  
Metal Ion ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Biochemical Properties ◽  
Antioxidant Enzyme Activities ◽  
Antioxidant Genes ◽  
Mitochondrial Functions ◽  
Myoblast Cells

Abstract Carnosine (β-alanyl-L-histidine) is a molecule naturally and exclusively present in muscle food with the highest concentrations found in skeletal muscles and brain of the animal. Among its numerous biochemical properties, carnosine has antioxidant activity which include metal ion chelation and free radical scavenging. We have recently reported that high muscle carnosine content in pig is associated with better meat quality. Moreover, supplementing pigs with β-alanine reduced oxidative damage to Longissimus muscle (LM) lipids and proteins. Among previously reported antioxidant activities, carnosine was found to limit the production of reactive oxygen species (ROS) and increase antioxidant enzyme activities. However, these studies were mainly conducted in rodents and cell lines and mechanisms in play remain to be characterized. To determine the effect of carnosine in preventing oxidative damage and characterize the mechanisms in play, we have undertaken experiments using the progeny (myoblasts) of satellite cells isolated from the LM of newborn piglets. Cells were treated with carnosine (0, 10, 25 and 50 mM) for 48 h and were then either collected immediately or treated with H2O2 (0.3 mM, 1 h) to induce an oxidative stress. Our results showed that carnosine prevents oxidative stress through the reduction of total intracellular ROS and by modulating the antioxidant system in myoblasts.Carnosine increased the mRNA abundance of NEF2L2, a transcription factor activated by oxidative stress, and several of its downstream regulated antioxidant genes. Western blot analyses further suggest that the protective effect of carnosine on H2O2-induced oxidative stress is mediated through the p38 MAPK intracellular pathway. Finally, the addition of carnosine to H2O2-treated myoblasts increased the basal cellular oxygen consumption rate (OCR), the ATP-linked OCR and proton leaks, thus suggesting an effect of carnosine on mitochondrial functions. Taken together, these findings demonstrate the important role of carnosine in preventing oxidative damage in porcine muscle cells.

Evaluation of the Potential Cardioprotective Activity of Some Saudi Plants against Doxorubicin Toxicity

2012 ◽  
Vol 67 (5-6) ◽  
pp. 297-307 ◽  
Author(s):  
Osama M. Ashour ◽  
Ashraf B. Abdel-Naim ◽  
Hossam M. Abdallah ◽  
Ayman A. Nagy ◽  
Ahmed M. Mohamadin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activities ◽  
Histopathological Examination ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Serum Markers ◽  
Protective Effects ◽  
Medicinal Uses ◽  
Cordia Myxa

Doxorubicin (DOX) is an anthracycline antibiotic widely used as a chemotherapeutic agent in the treatment of several tumours. However, its cardiac toxicity limits its use at maximum therapeutic doses. Most studies implicated increased oxidative stress as the major determinant of DOX cardiotoxicity. The local Saudi flora is very rich in a variety of plants of quite known folkloric or traditional medicinal uses. Tribulus macropterus Boiss., Olea europaea L. subsp. africana (Mill.) P. S. Green, Tamarix aphylla (L.) H. Karst., Cynomorium coccineum L., Cordia myxa L., Calligonum comosum L’ Hér, and Withania somnifera (L.) Dunal are Saudi plants known to have antioxidant activities. The aim of the current study was to explore the potential protective effects of methanolic extracts of these seven Saudi plants against DOX-induced cardiotoxicity in rats. Two plants showed promising cardioprotective potential in the order Calligonum comosum > Cordia myxa. The two plant extracts showed potent in vitro radical scavenging and antioxidant properties. They significantly protected against DOX-induced alterations in cardiac oxidative stress markers (GSH and MDA) and cardiac serum markers (CK-MB and LDH activities). Additionally, histopathological examination indicated a protection against DOX-induced cardiotoxicity. In conclusion, C. comosum and C. myxa exerted protective activity against DOX-induced cardiotoxicity, which is, at least partly, due to their antioxidant effect

Protective effects of polysaccharide from Euphorbia kansui (Euphorbiaceae) on the swimming exercise-induced oxidative stress in mice

2006 ◽  
Vol 84 (10) ◽  
pp. 1071-1079 ◽  
Author(s):  
Farong Yu ◽  
Shunqing Lu ◽  
Fahong Yu ◽  
Shutao Feng ◽  
Peter M. McGuire ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Activities ◽  
Oral Treatment ◽  
Swimming Exercise ◽  
Strenuous Exercise ◽  
Antioxidant Enzyme Activities ◽  
Protective Effects ◽  
Euphorbia Kansui ◽  
Exercise Induced

The present study examined the effects of derivatives of galactosides and glucosides in a polysaccharide extract from Euphorbia kansui (Euphorbiaceae) on exercise-induced oxidative stress in mice. Exhaustive swimming exercise significantly increases the degree of lipid peroxidation in terms of malondialdehyde content and reduces the antioxidant activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx). Our findings revealed that chronic oral treatment with the extract elevates enzymatic activities of SOD and GPx accompanied by a corresponding decrease in malondialdehyde. The antioxidative activities of these compounds against exercise-induced oxidative stress are correlated with various activities such as reducing the production of superoxide and hydroxyl radicals, inhibiting lipid peroxidation, enhancing antioxidative defenses, and increasing the production of SOD and GPx activity and expression in different tissues. These compounds may be involved in glycogen metabolism to meet the requirement of working skeletal muscles and act as antioxidants by terminating the chain reaction of lipid peroxidation to maintain the morphological stability of mitochondria in spinal motor neurons. These observations suggest that E. kansui has antioxidative and antifatigue properties and can be given as prophylactic and (or) therapeutic supplements for increasing antioxidant enzyme activities and preventing lipid peroxidation during strenuous exercise.

scholarly journals Alkyl Hydroxytyrosyl Ethers Show Protective Effects against Oxidative Stress in HepG2 Cells

2011 ◽  
Vol 59 (11) ◽  
pp. 5964-5976 ◽  
Author(s):  
Gema Pereira-Caro ◽  
Beatriz Sarriá ◽  
Andrés Madrona ◽  
José Luis Espartero ◽  
Luis Goya ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hepg2 Cells ◽  
Protective Effects

Moringa oleifera Leaf Extract Extends Lifespan and Ameliorate HAART Drug-Induced Locomotor, Reproductive, and Antioxidant Deficits in Drosophila melanogaster

2020 ◽  
pp. 33-46
Author(s):  
Walter Mdekera Iorjiim ◽  
Simeon Omale ◽  
Great David Bagu ◽  
Steven Samuel Gyang ◽  
Emmanuel Taiwo Alemika
Keyword(s):  
Oxidative Stress ◽  
Drosophila Melanogaster ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
Antioxidant Activities ◽  
Statistical Significance ◽  
P Value ◽  
Drug Induced ◽  
Mda Content ◽  
Biomarkers Of Oxidative Stress

Aim: To evaluate the longevity and ameliorative activities of Moringa oleifera leaf (MOL) extract against some HAART drug-induced toxicities in Drosophila melanogaster. Materials and Methods: The research was conducted at the Drosophila laboratory, Africa Centre of Excellence in phytomedicine Research and Development (ACEPRD), University of Jos-Nigeria, between August 2019 - March 2020.  D. melanogaster (1-3 day) were first exposed for life to different concentrations of MOL (50 – 500 mg) or 25 mM Ascorbic acid or 1000 µL distilled water to determine longevity. Secondly, flies were fed on 46.56 mg of HAART drugs (Efavirenz-based or Dolutegravir-based) alone or supplemented with MOL 250 mg or 500 mg per 10 g fly food in five replicates for seven days. Afterward, longevity, fecundity, and negative geotaxis were evaluated. Also, activities of Superoxide dismutase, Catalase, as well as Malondialdehyde content as biomarkers of oxidative stress were evaluated using whole fly homogenate. Statistical significance was taken at P<0.05 or (P<0.006) (Bonferroni adjusted P-value for multiple curve comparisons.  Results: The MOL extract significantly (P<0.001) increased fly longevity compared to control groups. Similarly, supplementation with 500 mg MOL extracts significantly (P<0.05) ameliorate HAART drug-induced deficits in climbing ability, fecundity, SOD, and CAT activities as well as MDA content compared to groups exposed to HAART drugs alone respectively. Conclusion: The results suggest that   M. oleifera leaf extract extends lifespan and ameliorate HAART drug-induced toxicities via its antioxidant activities. This was supported by improved locomotor and reproductive decline, and restoration of the deficits in the biomarkers of oxidative stress (SOD, CAT, and MDA) in D. melanogaster.

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