Changes in Lutein Status Markers (Serum and Faecal Concentrations, Macular Pigment) in Response to a Lutein-Rich Fruit or Vegetable (Three Pieces/Day) Dietary Intervention in Normolipemic Subjects

Lutein is mainly supplied by dietary fruit and vegetables, and they are commonly jointly assessed in observational and interventional studies. Lutein bioavailability and health benefits depend on the food matrix. This study aimed to assess the effect of dietary intervention with lutein-rich fruit or vegetables on lutein status markers, including serum and faecal concentrations (by high pressure liquid chromatography), dietary intake (24 h recalls ×3), and macular pigment optical density (MPOD) and contrast threshold (CT) as visual outcomes. Twenty-nine healthy normolipemic subjects, aged 45–65 y, consumed 1.8 mg lutein/day supplied from fruits (14 subjects, 500 g/day of oranges, kiwi and avocados) or vegetables (15 subjects, 180 g/day of green beans, pumpkin, and sweet corn) for four weeks. Serum lutein concentration increased by 37%. The effect of the food group intervention was statistically significant for serum lutein+zeaxanthin concentration (p = 0.049). Serum α- and β-carotene were influenced by food type (p = 0.008 and p = 0.005, respectively), but not by time. Serum lutein/HDL-cholesterol level increased by 29% (total sample, p = 0.008). Lutein+zeaxanthin/HDL-cholesterol increased, and the intervention time and food group eaten had an effect (p = 0.024 and p = 0.010, respectively) which was higher in the vegetable group. The MPOD did not show variations, nor did it correlate with CT. According to correlation matrixes, serum lutein was mainly related to lutein+zeaxanthin expressed in relation to lipids, and MPOD with the vegetable group. In faecal samples, only lutein levels increased (p = 0.012). This study shows that a relatively low amount of lutein, supplied by fruit or vegetables, can have different responses in correlated status markers, and that a longer intervention period is needed to increase the MPOD. Therefore, further study with larger sample sizes is needed on the different responses in the lutein status markers and on food types and consumption patterns in the diet, and when lutein in a “pharmacological dose” is not taken to reduce a specific risk.

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Predictors of macular pigment and contrast threshold in normolipemic subjects aged 45-65

10.21203/rs.3.rs-22739/v1 ◽

2020 ◽

Cited By ~ 1

Author(s):

Begoña Olmedilla-Alonso ◽

Elena Rodríguez-Rodríguez ◽

Beatriz Beltrán-de-Miguel ◽

Rocío Estévez-Santiago ◽

Milagros Sánchez-Prieto

Keyword(s):

Dietary Intake ◽

High Performance ◽

Vegetable Consumption ◽

Hdl Cholesterol ◽

Visual Outcome ◽

Macular Pigment ◽

Contrast Threshold ◽

Serum Concentrations ◽

Heterochromatic Flicker Photometry ◽

Relevant Role

Abstract Objective: The dietary carotenoids lutein and zeaxanthin are transported in the bloodstream by lipoproteins and selectively captured in the retina where they constitute macular pigment. There are no lutein and zeaxanthin dietary intake recommendations nor desired blood/tissue concentrations for the general population. The aim of this study was to determine the lutein and zeaxanthin dietary intake, their serum concentrations, lipid profile, macular pigment optical density (MPOD) and the contrast sensitivity (CT), as visual outcome in normolipemic subjects age 45-65 (n=101). Methods: MPOD, L and Z in serum and dietary intake were determined using heterochromatic flicker photometry, high-performance liquid chromatography and 3-day food records. CT was measured with the CGT-1000 Contrast Glaretester at six stimulus sizes, with and without glare. Results: Lutein and zeaxanthin serum concentrations (median): 0.361 and 0.078 µmol/L. Lutein+zeaxanthin intake: 1.1 mg/d (median). MPOD: 0.34 du. Lutein+zeaxanthin intake correlates with their serum concentrations (ρ=0.333, p =0.001), which in turn correlates with MPOD (ρ=0.229, p =0.000) and with the fruit and vegetable consumption (ρ=0.202, p =0.001), but not with the lutein+zeaxanthin dietary intake. HDL-cholesterol correlated with lutein+zeaxanthin serum (ρ=0.253, p =0.000) and with CT under glare conditions (ρ range: 0.016–0.160). MPOD predictors: serum lutein+zeaxanthin, lutein+zeaxanthin/HDL-cholesterol and HDL-cholesterol (R 2 =15.9%). CT predictors: MPOD and sex (β coefficients ranges: -0.950,-0.392; -0.134,-0.393, respectively). Conclusion: There were correlations at all points in time in this sequence between lutein+zeaxanthin intake and the visual outcome and, HDL-cholesterol played a relevant role.

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Predictors of macular pigment and contrast threshold in Spanish healthy normolipemic subjects (45–65 years) with habitual food intake

PLoS ONE ◽

10.1371/journal.pone.0251324 ◽

2021 ◽

Vol 16 (5) ◽

pp. e0251324

Author(s):

Begoña Olmedilla-Alonso ◽

Elena Rodríguez-Rodríguez ◽

Beatriz Beltrán-de-Miguel ◽

Rocío Estévez-Santiago ◽

Milagros Sánchez-Prieto

Keyword(s):

Dietary Intake ◽

Contrast Sensitivity ◽

Vegetable Consumption ◽

Hdl Cholesterol ◽

Visual Outcome ◽

Macular Pigment ◽

Contrast Threshold ◽

Serum Concentrations ◽

Spatial Frequencies ◽

Lower Ratio

Introduction The dietary carotenoids lutein (L) and zeaxanthin (Z) are transported in the bloodstream by lipoproteins, sequestered by adipose tissue, and eventually captured in the retina where they constitute macular pigment. There are no L&Z dietary intake recommendations nor desired blood/tissue concentrations for the Spanish general population. Our aim was to assess the correlation of L&Z habitual dietary intake (excluding food supplements), resulting serum concentrations and lipid profile with macular pigment optical density (MPOD) as well as the contrast sensitivity (CT), as visual outcome in normolipemic subjects (n = 101) aged 45–65. Methods MPOD was measured by heterochromatic flicker photometry, serum L&Z by HPLC, the dietary intake by a 3-day food records and CT using the CGT-1000-Contrast-Glaretester at six stimulus sizes, with and without glare. Results Lutein and zeaxanthin concentrations (median) in serum: 0.361 and 0.078 μmol/L, in dietary intake: 1.1 mg L+Z/day. MPOD: 0.34du. L+Z intake correlates with their serum concentrations (rho = 0.333, p = 0.001), which in turn correlates with MPOD (rho = 0.229, p = 0.000) and with fruit and vegetable consumption (rho = 0.202, p = 0.001), but not with lutein+zeaxanthin dietary intake. MPOD correlated with CT, with and without glare (rho ranges: -0.135, 0.160 and -0.121, –0.205, respectively). MPOD predictors: serum L+Z, L+Z/HDL-cholesterol (β-coeficient: -0.91±0.2, 95%CI: -1.3,-0.5) and HDL-cholesterol (R2 = 15.9%). CT predictors: MPOD, mainly at medium and smaller visual angles (corresponding to spatial frequencies for which sensitivity declines with age) and gender (β-coefficients ranges: -0.95,-0.39 and -0.13,-0.39, respectively). Conclusion A higher MPOD is associated with a lower ratio of L+Z/HDL-cholesterol and with a lower CT (higher contrast sensitivity). The HDL-cholesterol would also act indirectly on the CT improving the visual function.

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Effects of Indigenous Diet Iron Content and Location on Hemoglobin Levels of Ghanaians

Nutrients ◽

10.3390/nu12092710 ◽

2020 ◽

Vol 12 (9) ◽

pp. 2710 ◽

Cited By ~ 1

Author(s):

April Callister ◽

Joanna Gautney ◽

Christina Aguilar ◽

Julian Chan ◽

David Aguilar

Keyword(s):

Food Group ◽

Food Type ◽

Plant Proteins ◽

Food Groups ◽

Sex Distribution ◽

Dietary Interventions ◽

Food Frequency ◽

Blood Samples ◽

Ease Of Access ◽

The Impact

Multiple studies have demonstrated strong links between diet and anemia, but few have explored the impact of food groups on hemoglobin (Hb). We analyzed the correlation between Ghanaian diet and Hb levels to explore reduction of anemia prevalence through dietary interventions. Demographics, food frequency questionnaires (FFQ), and blood samples were obtained from 140 volunteers (ages 18–65) in five locations across Ghana. Hb was measured; estimated iron consumption was calculated. FFQ items were grouped by food type, and a regression analysis was performed to determine the most important dietary predictors of Hb. Moreover, 47% of total participants were anemic; 64% of females and 28% of males. Hb levels were highest in Mole (13.9 g/dL, SD = ±1.9), independent of sex distribution. The regression model revealed a 62.7% adjusted correlation between food groups and Hb levels. Animal foods (β = 0.016, t = 5.08, p < 0.01) and plant protein (β = 0.013, t = 2.86, p < 0.01) were the most influential groups to Hb levels. It is of vital importance to emphasize the benefits of consuming animal foods and plant proteins within the Ghanaian population. The ease of access to plant proteins makes it likely that this food group will be most influential and have the greatest impact in reduction of anemia in the Ghanaian population.

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Consumption of either one egg or lutein‐enriched egg per day increases HDL cholesterol, reduces apolipoprotein B while increasing plasma carotenoids and macular pigment density in adult subjects

The FASEB Journal ◽

10.1096/fasebj.24.1_supplement.92.4 ◽

2010 ◽

Vol 24 (S1) ◽

Author(s):

Shymaa Ata ◽

Jacqueline Barona ◽

Rachel Kopec ◽

Jenifer Jones ◽

Mariana Calle ◽

...

Keyword(s):

Apolipoprotein B ◽

Hdl Cholesterol ◽

Macular Pigment

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Assurance Polyclonal Enzyme Immunoassay for Detection of Listeria monocytogenes and Related Listeria Species in Selected Foods: Collaborative Study

Journal of AOAC International ◽

10.1093/jaoac/80.4.775 ◽

1997 ◽

Vol 80 (4) ◽

pp. 775-790 ◽

Cited By ~ 15

Author(s):

Philip T Feldsine ◽

Andrew H Lienau ◽

Robin L Forgey ◽

Roger D Calhoon ◽

S Al-Hasani ◽

...

Keyword(s):

Listeria Monocytogenes ◽

Enzyme Immunoassay ◽

Collaborative Study ◽

Culture Method ◽

The United States ◽

Food Type ◽

Green Beans ◽

Private Industry ◽

Listeria Species ◽

Two Samples

Abstract Six foods representing a variety of food products were analyzed by the Assurance Listeria polyclonal enzyme immunoassay (EIA) and by either the Bacteriological Analytical Manual or the U.S. Department of Agriculture culture method for detecting Listeria monocytogenes and related Listeria species. Samples of each food type, at each inoculation level, were analyzed simultaneously by both methods. A total of 19 laboratories representing federal government agencies and private industry in the United States and Canada participated. Food types were inoculated with Listeria species including L. monocytogenes, with the exception of 3 lots of green beans, which were naturally contaminated. During this study, 1764 samples and controls were analyzed and confirmed, of which 492 were positive and 947 were negative by both methods. There were 159 samples that were positive by culture method but negative by the EIA and 188 that were negative by culture method but positive by EIA. Twenty-two samples were negative by EIA and by culture method but confirmed positive when Assurance selective enrichment broths were subcultured to selective agar. The Assurance polyclonal EIA for detecting L. monocytogenes and related Listeria species in foods has been adopted first action by AOAC INTERNATIONAL.

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Development of a Genetic Score to Predict an Increase in HDL Cholesterol Concentration After a Dietary Intervention in Adults with Metabolic Syndrome

Journal of Nutrition ◽

10.1093/jn/nxz060 ◽

2019 ◽

Vol 149 (7) ◽

pp. 1116-1121 ◽

Cited By ~ 1

Author(s):

Martha Guevara-Cruz ◽

Isabel Medina-Vera ◽

Adriana Flores-López ◽

Miriam Aguilar-López ◽

Caren E Smith ◽

...

Keyword(s):

Metabolic Syndrome ◽

Dietary Intervention ◽

Saturated Fat ◽

Hdl Cholesterol ◽

Cholesterol Concentration ◽

Genetic Score ◽

Before And After ◽

Low Hdl ◽

Genetic Predisposition Score ◽

Mexican Adults

ABSTRACT Background Dietary intervention (DI) is a primary strategy to attenuate some of the metabolic abnormalities associated with metabolic syndrome (MetS), including low HDL cholesterol. There is no biomarker that can identify individuals who respond to DI by increasing HDL cholesterol. Objective The aim of this study was to assess the predictive power of a genetic predisposition score (GPS) in Mexican adults with MetS to identify HDL cholesterol responders to DI. Methods This study followed a prospective cohort design. Sixty-seven Mexican adults aged 20–60 y (21% men) with BMI ≥25 and ≤39.9 kg/m², who had at least 3 of 5 positive criteria for MetS, were included. Participants consumed a low saturated fat diet for 2.5 mo (<7% energy as saturated fat, <200 mg of cholesterol/d) and reduced their usual diet by ∼440 kcal/d, a reduction in total energy intake of about 25%. Anthropometry and serum biochemical markers, including HDL cholesterol, were measured before and after DI. A multilocus GPS was constructed using previously reported genetic variants associated with response to diet in subjects with MetS. GPS values, designed to predict the response of HDL cholesterol to the DI, were computed for each individual as the sum of the number of effect alleles across 14 SNPs. Results Individuals were dichotomized as high and low GPS according to median GPS (−2.12) and we observed a difference in HDL cholesterol changes on DI of +3 mg/dL (6.3%) in subjects with low GPS, whereas those with high GPS had HDL cholesterol decreases of −3 mg/dL (−7.9%) (P = 0.04). Conclusions Individuals with low GPS showed greater increases in their HDL cholesterol than those with high GPS. Therefore, the GPS can be useful for predicting the HDL cholesterol response to diet.

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Influence of SNPs in nutrient-sensitive candidate genes and gene–diet interactions on blood lipids: the DiOGenes study

British Journal Of Nutrition ◽

10.1017/s0007114512006058 ◽

2013 ◽

Vol 110 (5) ◽

pp. 790-796 ◽

Cited By ~ 9

Author(s):

Lena K. Brahe ◽

Lars Ängquist ◽

Lesli H. Larsen ◽

Karani S. Vimaleswaran ◽

Jörg Hager ◽

...

Keyword(s):

Lipid Metabolism ◽

Candidate Genes ◽

Dietary Protein ◽

Multiple Testing ◽

Dietary Intervention ◽

Weight Maintenance ◽

Hdl Cholesterol ◽

Blood Lipid ◽

Glycaemic Index ◽

Lipid Response

Blood lipid response to a given dietary intervention could be determined by the effect of diet, gene variants or gene–diet interactions. The objective of the present study was to investigate whether variants in presumed nutrient-sensitive genes involved in lipid metabolism modified lipid profile after weight loss and in response to a given diet, among overweight European adults participating in the Diet Obesity and Genes study. By multiple linear regressions, 240 SNPs in twenty-four candidate genes were investigated for SNP main and SNP–diet interaction effects on total cholesterol, LDL-cholesterol, HDL-cholesterol and TAG after an 8-week low-energy diet (only main effect), and a 6-monthad libitumweight maintenance diet, with different contents of dietary protein or glycaemic index. After adjusting for multiple testing, a SNP–dietary protein interaction effect on TAG was identified for lipin 1 (LPIN1) rs4315495, with a decrease in TAG of − 0·26 mmol/l per A-allele/protein unit (95 % CI − 0·38, − 0·14,P= 0·000043). In conclusion, we investigated SNP–diet interactions for blood lipid profiles for 240 SNPs in twenty-four candidate genes, selected for their involvement in lipid metabolism pathways, and identified one significant interaction betweenLPIN1rs4315495 and dietary protein for TAG concentration.

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Peas, Sweet Corn, and Green Beans

Handbook of Vegetables and Vegetable Processing ◽

10.1002/9781119098935.ch33 ◽

2018 ◽

pp. 761-783

Author(s):

Muhammad Siddiq ◽

Melvin A. Pascall

Keyword(s):

Sweet Corn ◽

Green Beans

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SARI KACANG HIJAU SEBAGAI ALTERNATIF MENINGKATKAN PRODUKSI AIR SUSU IBU (ASI) PADA IBU MENYUSUI

JURNAL KEPERAWATAN DAN FISIOTERAPI (JKF) ◽

10.35451/jkf.v2i1.272 ◽

2019 ◽

Vol 2 (1) ◽

pp. 89-94

Author(s):

Nikmah Jalilah Ritonga ◽

Eka Defi Mulyani ◽

Diah Evawanna Anuhgera ◽

Damayanti Damayanti ◽

Riris Sitorus ◽

...

Keyword(s):

Breast Milk ◽

Milk Production ◽

Food Source ◽

Total Sample ◽

Wilcoxon Test ◽

Vulnerable Groups ◽

Green Beans ◽

Breast Milk Production ◽

Nursing Mothers ◽

Equality Test

Abstract Nursing mothers belong to one of the vulnerable groups of nutrition, because breast milk which is the main food for babies is obtained from the mother. Therefore, mothers who are breastfeeding must be controled to the intake of nutrients consumed. green beans as a food source of vegetable protein which has been very popular in everyday life. Based on the amount every 100 grams, protein is the second main constituent after calories, which contains 22.2% protein. The purpose of this study was to determine the effect of consumption of mungbean juice on breast milk production in nursing mothers. This research is a quantitative study with a pre-experimental design with one group pretest posttest, with a population of 20 breastfeeding mothers (0-2 months) and a total sample of 11 nursing mothers who delivered at Klinik Pratama Tutun Sehati. Data collection uses an observation sheet using indicators of Smooth Milk Production. The characteristic equality test showed that there was an effect of consuming mung bean extract on breast milk production in nursing mothers (p> α, α≤0.05). The results of the analysis test with the Wilcoxon test showed that there was a smooth effect of breast milk production (p = 0.046). It was recommended for nursing mothers whose milk was not smooth to pay attention to nutritional intake in order to improve the quality and amount of milk production, in packaging because the price is also affordable.

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