scholarly journals Gum Acacia Functionalized Colloidal Gold Nanoparticles of Letrozole as Biocompatible Drug Delivery Carrier for Treatment of Breast Cancer

Pharmaceutics ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1554
Author(s):  
Hibah M. Aldawsari ◽  
Sima Singh ◽  
Nabil A. Alhakamy ◽  
Rana B. Bakhaidar ◽  
Abdulrahman A. Halwani ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Drug Delivery ◽  
Gold Nanoparticles ◽  
Postmenopausal Women ◽  
Specific Binding ◽  
Cancer Cell Line ◽  
Good Alternative ◽  
Inorganic Nanoparticles ◽  
Gum Acacia ◽  
Drug Delivery Carrier

The most prevalent malignancy among postmenopausal women is breast cancer. It is one of the leading causes of cancer-related mortality among women. Letrozole (LTZ) is a clinically approved inhibitor for breast cancer in postmenopausal women. However, due to poor aqueous solubility, non-specific binding, unwanted toxicity, and poor blood circulation hampered its clinical applications. To maximize the pharmacological effects and minimize the side effects, inorganic nanoparticles are a good alternative. Due to excellent biocompatibility and minimum cytotoxicity, gold nanoparticles (AuNPs) offer distinct benefits over other metal nanoparticles. Emerging as attractive components, AuNPs and Gum acacia (GA) have been extensively studied as biologically safe nanomaterials for the treatment of cancers. This study reports the synthesis and characterization of GA stabilized gold nanoparticles (GA-AuNPs) of LTZ for breast cancer treatment. The observed particle size of optimized LTZ @ GA-AuNPs was 81.81 ± 4.24 nm in size, 0.286 ± 0.143 of polydispersity index (PDI) and −14.6 ± −0.73 mV zeta potential. The biologically synthesized LTZ @ GA-AuNPs also demonstrated dose-dependent cytotoxicity against the human breast cancer cell line MCF-7, with an inhibitory concentration (IC50) of 3.217 ± 0.247. We determined the hemolytic properties of the LTZ @ GA-AuNPs to evaluate the interaction between the nanoparticles and blood components. Results showed that there is no interaction between LTZ @ GA-AuNPs and blood. In conclusion, the findings indicate that LTZ @ GA-AuNPs has significant potential as a promising drug delivery carrier for treating breast cancer in postmenopausal women.

scholarly journals EVALUATION OF [11C]MPC-6827 AS A MICROTUBULE TARGETING PET RADIOTRACER IN CANCER CELL LINES

2019 ◽  
pp. 43-47
Author(s):  
J. S. DILEEP KUMAR ◽  
JAYA PRABHAKARAN ◽  
NARESH DAMUKA ◽  
JUSTIN W. HINES ◽  
STEVEN J. KRIDEL ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Prostate Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Specific Binding ◽  
Cancer Cell Line ◽  
Prostate Cancer Cell Line ◽  
Microtubule Targeting Agents ◽  
Pc3 Cells

Objective: The objective of this study was to evaluate the uptake and specificity of [11C]MPC-6827, a MT targeted PET ligand in prostate, glioblastoma and breast cancer cells. Methods: [11C]MPC-6827 was synthesized by reacting corresponding desmethyl precursors with [11C]CH3I in a GE-FX2MeI/FX2M radiochemistry module. In vitro binding of [11C]MPC-6827 was performed in breast cancer MDA-MB-231, glioblastoma (GBM) patient-derived tumor (GBM-PDX), GBM U251 and prostate cancer 3 (PC3) cell lines at 37 °C in quadruplicate at 5, 15, 30, 60, and 90 minute incubation time. The nonspecific bindings were determined by incubation with unlabeled microtubule targeting agents MPC-6827, HD-800, colchicine, paclitaxel and docetaxel (5.0 mM). Results: [11C]MPC-6827 provided the highest binding in the breast cancer cell, MDA-MB-231, among all the cells studied, with 90% specific binding. [11C]MPC-6827 binds to glioblastoma PDX and U251 cells with ~50% and 40% specific binding, whereas, prostate cancer cell line, PC3 cells showed 40% specific binding. [11C]MPC-6827 also exhibits binding to the taxane and colchicine binding sites of MTs, in MDA-MB-231 cells. Conclusion: These data indicate that [11C]MPC-6827 can be a promising PET radiotracer for preclinical imaging of the brain and peripheral cancers.

scholarly journals Programmable Ce6 Delivery via Cyclopamine Based Tumor Microenvironment Modulating Nano-System for Enhanced Photodynamic Therapy in Breast Cancer

2019 ◽  
Vol 7 ◽  
Author(s):  
Chan Feng ◽  
Lv Chen ◽  
Yonglin Lu ◽  
Jie Liu ◽  
Shujing Liang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Drug Delivery ◽  
Photodynamic Therapy ◽  
Tumor Microenvironment ◽  
Cancer Cell Line ◽  
Chlorin E6 ◽  
Great Promise ◽  
Tissue Destruction ◽  
Mice Model ◽  
Cell Internalization

Photodynamic therapy (PDT) has shown great promise in breast cancer treatment. However, simplex target ligand modification or stimuli release cannot meet the requirement of effective drug delivery to solid tumor tissue. To overcome continuous bio-barriers existing in the tumor microenvironment, multi-stage response drug delivery was desirable. Herein, we developed a unique tumor microenvironment tailored nanoplatform for chlorin e6 (Ce6) delivery. We chose bovine serum albumin (BSA) as “mother ships” material for effective tumor periphery resident, cyclopamine (CYC) as extracellular matrix (ECM) inhibitor and synergistic anti-tumor agent, and diselenide containing amphiphilic hyaluronic acid-chlorin e6 polymers (HA-SeSe-Ce6) synthesized as “small bombs” for internal tissue destruction. The above three distinct function compositions were integrated into an independent CYC and HA-SeSe-Ce6 co-delivery albumin nano-system (ABN@HA-SeSe-Ce6/CYC). The obtained nano-system presents good biocompatible, long circulation and effective tumor accumulation. After entering tumor microenvironment, CYC gradually releases to disrupt the ECM barrier to open the way for further penetration of HA-SeSe-Ce6. Subsequently, targeted tumor cell internalization and intracellular redox response release of Ce6 would achieve. Moreover, CYC could also make up the deficiency of Ce6 in hypoxia area, owing to its anti-tumor effect. Improved therapeutic efficacy was verified in a breast cancer cell line and tumor-bearing mice model.

Semiconductor Quantum Dots for Cell Imaging

2009 ◽  
Vol 1237 ◽  
Author(s):  
Zoraida Pascual Aguilar ◽  
Hengyi Xu ◽  
Ben Jones ◽  
John Dixon ◽  
Andrew Wang
Keyword(s):  
Breast Cancer ◽  
Quantum Dots ◽  
Cell Surface ◽  
Cell Imaging ◽  
Specific Binding ◽  
Life Sciences ◽  
Cancer Cell Line ◽  
Gold Nanocrystals

AbstractNanotechnology is currently undergoing unprecedented development in various fields. There has been a widespread interest in the application of nanomaterials in medicine with its promise of improving imaging, diagnostics, and therapy. The recent advances in engineering and technology have led to the development of new nanoscale platforms such as quantum dots, gold nanocrystals, superparamagnetic nanocrystals, and other semiconductor nanoparticles. Literature on the applications of quantum dots in life sciences has recently increased in number. This may have led to predictions that nanotechnology in life sciences research will contribute $3.4 billion by 2010 while institutions have predicted that the market for nanotechnology and corresponding products will reach $1 trillion in 2012 (1).Ocean NanoTech is at the height of developmental stages of nanoparticle production for biological applications. Ocean’s high quantum-yield quantum dots (QDs) is currently being tested and used for cell imaging, as wells as for the detection of proteins, DNA, whole cells, and whole organisms. Imaging of cells involves conjugation of QDs to highly sensitive and specific antibody to form QD˜Ab conjugates that attach to specific protein target on the cell surface. Attachment of the QD˜Ab on the cell surface allows imaging of the cell under a fluorescence microscope. QD based imaging can be used in a multiplex immunoassay detection of several types of cells (or microorganisms) in a single sample when several size tunable quantum dots are used as reporter probes.We report the QD imaging of breast cancer cells. Using the breast cancer cell line SK-BR3, which expresses high levels of her2 antigens on the cell surface, anti-her2 were conjugated to Ocean’s quantum dots, QSH620. To eliminate non-specific binding of the QD˜20Ab Ocean’s super blocking buffer BBB and BBG were used. Preliminary results of in vitro studies indicated that QD based systems can be used to image cells. We anticipate that this system can be transferred to in vivo detection.

The improvement of anti-proliferation activity against breast cancer cell line of thioguanine by gold nanoparticles

2012 ◽  
Vol 22 (1) ◽  
pp. 303-311 ◽  
Author(s):  
Sadaf Aghevlian ◽  
Reza Yousefi ◽  
Reza Faghihi ◽  
Abdolkarim Abbaspour ◽  
Ali Niazi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gold Nanoparticles ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
Proliferation Activity

scholarly journals Construction of an exosome-functionalized graphene oxide based composite bionic smart drug delivery system and its anticancer activity

2022 ◽  
Author(s):  
Qi Chen ◽  
Chengchuan Che ◽  
Jinfeng Liu ◽  
Zhijin Gong ◽  
Meiru Si ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Drug Delivery ◽  
Graphene Oxide ◽  
Drug Delivery System ◽  
Delivery System ◽  
Functionalized Graphene Oxide ◽  
Ph Response ◽  
Drug Delivery Carrier ◽  
Cancer Tumor

Abstract Graphene oxide has covalently modified by chito oligosaccharides and γ-polyglutamic acid to form GO-CO-γ-PGA, which exhibits excellent performance as a drug delivery carrier, but this carrier did not have the ability to actively target. In this study, the targeting property of breast cancer tumor cell exosomes was exploited to give GO-CO-γ-PGA the ability to target breast tumor cells (MDA-MB-231), and the drug mitoxantrone (MIT) was loaded to finally form EXO-GO-CO-γ-PGA-MIT with a loading capacity of 1.39 mg/mg. The pH response of EXO-GO-CO-γ-PGA showed a maximum cumulative release rate of 56.59% (pH 5.0) and 6.73% (pH 7.4) for MIT at different pH conditions. pH 7.4). In vitro cellular assays showed that EXO-GO-CO-γ-PGA-MIT was more potent in killing MDA-MB-231 cells due to its targeting ability and had a significantly higher pro-apoptotic capacity compared to GO-CO-γ-PGA-MIT. The results showed that this bionic nano-intelligent drug delivery system has good drug slow release function, can increase the local drug concentration of tumor and enhance the pro-apoptotic ability of MIT, so this newly synthesized bionic drug delivery carriers (EXO-GO-CO-γ-PGA-MIT) has potential application in breast cancer treatment.

scholarly journals Novel pH-sensitive and biodegradable micelles for the combined delivery of doxorubicin and conferone to induce apoptosis in MDA-MB-231 breast cancer cell line

RSC Advances ◽  
2020 ◽  
Vol 10 (49) ◽  
pp. 29228-29246 ◽  
Author(s):  
Akram Rahmani ◽  
Hassan Zavvar Mousavi ◽  
Roya Salehi ◽  
Ahmad Bagheri
Keyword(s):  
Breast Cancer ◽  
Drug Delivery ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Chemotherapy ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
Ph Sensitive ◽  
Line P

pH-sensitive micelles are desirable for co-drug delivery in cancer chemotherapy.

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