scholarly journals Arabidopsis CCoAOMT1 Plays a Role in Drought Stress Response via ROS- and ABA-Dependent Manners

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 831
Author(s):  
Hyun-Jin Chun ◽  
Lack-Hyeon Lim ◽  
Mi-Sun Cheong ◽  
Dongwon Baek ◽  
Mi-Suk Park ◽  
...  
Keyword(s):  
Drought Stress ◽  
Stress Response ◽  
Methyl Viologen ◽  
Saline Stress ◽  
Loss Of Function ◽  
Wild Type ◽  
Morphological Adaptation ◽  
Positive Role ◽  
Aba Biosynthesis ◽  
Seed Germination Assay

Plants possess adaptive reprogramed modules to prolonged environmental stresses, including adjustment of metabolism and gene expression for physiological and morphological adaptation. CCoAOMT1 encodes a caffeoyl CoA O-methyltransferase and is known to play an important role in adaptation of Arabidopsis plants to prolonged saline stress. In this study, we showed that the CCoAOMT1 gene plays a role in drought stress response. Transcript of CCoAOMT1 was induced by salt, dehydration (drought), and methyl viologen (MV), and loss of function mutants of CCoAOMT1, ccoaomt1-1, and ccoaomt1-2 exhibit hypersensitive phenotypes to drought and MV stresses. The ccoaomt1 mutants accumulated higher level of H2O2 in the leaves and expressed lower levels of drought-responsive genes including RD29B, RD20, RD29A, and ERD1, as well as ABA3 3 and NCED3 encoding ABA biosynthesis enzymes during drought stress compared to wild-type plants. A seed germination assay of ccoaomt1 mutants in the presence of ABA also revealed that CCoAOMT1 functions in ABA response. Our data suggests that CCoAOMT1 plays a positive role in response to drought stress response by regulating H2O2 accumulation and ABA signaling.

scholarly journals Overexpression of the pitaya phosphoethanolamine N-methyltransferase gene (HpPEAMT) enhanced simulated drought stress in tobacco

2021 ◽  
Author(s):  
Ai-Hua Wang ◽  
Lan Yang ◽  
Xin-Zhuan Yao ◽  
Xiao-Peng Wen
Keyword(s):  
Drought Stress ◽  
Stress Response ◽  
Transgenic Plants ◽  
Drought Tolerance ◽  
Transgenic Tobacco ◽  
Sequence Similarity ◽  
Amino Acid Sequence Similarity ◽  
Transgenic Tobacco Plants ◽  
Wild Type ◽  
Tobacco Plants

AbstractPhosphoethanolamine N-methyltransferase (PEAMTase) catalyzes the methylation of phosphoethanolamine to produce phosphocholine and plays an important role in the abiotic stress response. Although the PEAMT genes has been isolated from many species other than pitaya, its role in the drought stress response has not yet been fully elucidated. In the present study, we isolated a 1485 bp cDNA fragment of HpPEAMT from pitaya (Hylocereus polyrhizus). Phylogenetic analysis showed that, during its evolution, HpPEAMT has shown a high degree of amino acid sequence similarity with the orthologous genes in Chenopodiaceae species. To further investigate the function of HpPEAMT, we generated transgenic tobacco plants overexpressing HpPEAMT, and the transgenic plants accumulated significantly more glycine betaine (GB) than did the wild type (WT). Drought tolerance trials indicated that, compared with those of the wild-type (WT) plants, the roots of the transgenic plants showed higher drought tolerance ability and exhibited improved drought tolerance. Further analysis revealed that overexpression of HpPEAM in Nicotiana tabacum resulted in upregulation of transcript levels of GB biosynthesis-related genes (NiBADH, NiCMO and NiSDC) in the leaves. Furthermore, compared with the wild-type plants, the transgenic tobacco plants displayed a significantly lower malondialdehyde (MDA) accumulation and higher activities of the superoxide dismutase (SOD) and peroxidase (POD) antioxidant enzymes under drought stress. Taken together, our results suggested that HpPEAMT enhanced the drought tolerance of transgenic tobacco.

The NADPH-oxidase AtRbohI plays a positive role in drought-stress response in Arabidopsis thaliana

2017 ◽  
Vol 491 (3) ◽  
pp. 834-839 ◽  
Author(s):  
Huan He ◽  
Jingwei Yan ◽  
Xiaoyun Yu ◽  
Yan Liang ◽  
Lin Fang ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Drought Stress ◽  
Stress Response ◽  
Nadph Oxidase ◽  
Positive Role

scholarly journals Brassica Rapa SR45a Regulates Drought Tolerance via the Alternative Splicing of Target Genes

Genes ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 182 ◽  
Author(s):  
Muthusamy ◽  
Yoon ◽  
Kim ◽  
Jeong ◽  
Lee
Keyword(s):  
Alternative Splicing ◽  
Drought Stress ◽  
Stress Response ◽  
Drought Tolerance ◽  
Brassica Rapa ◽  
Target Genes ◽  
Tolerance Index ◽  
Dependent Manner ◽  
Loss Of Function ◽  
Splicing Patterns

The emerging evidence has shown that plant serine/arginine-rich (SR) proteins play a crucial role in abiotic stress responses by regulating the alternative splicing (AS) of key genes. Recently, we have shown that drought stress enhances the expression of SR45a (also known as SR-like 3) in Brassica rapa. Herein, we unraveled the hitherto unknown functions of BrSR45a in drought stress response by comparing the phenotypes, chlorophyll a fluorescence and splicing patterns of the drought-responsive genes of Arabidopsis BrSR45a overexpressors (OEs), homozygous mutants (SALK_052345), and controls (Col-0). Overexpression and loss of function did not result in aberrant phenotypes; however, the overexpression of BrSR45a was positively correlated with drought tolerance and the stress recovery rate in an expression-dependent manner. Moreover, OEs showed a higher drought tolerance index during seed germination (38.16%) than the control lines. Additionally, the overexpression of BrSR45a induced the expression of the drought stress-inducible genes RD29A, NCED3, and DREB2A under normal conditions. To further illustrate the molecular linkages between BrSR45a and drought tolerance, we investigated the AS patterns of key drought-tolerance and BrSR45a interacting genes in OEs, mutants, and controls under both normal and drought conditions. The splicing patterns of DCP5, RD29A, GOLS1, AKR, U2AF, and SDR were different between overexpressors and mutants under normal conditions. Furthermore, drought stress altered the splicing patterns of NCED2, SQE, UPF1, U4/U6-U5 tri-snRNP-associated protein, and UPF1 between OEs and mutants, indicating that both overexpression and loss of function differently influenced the splicing patterns of target genes. This study revealed that BrSR45a regulates the drought stress response via the alternative splicing of target genes in a concentration-dependent manner.

scholarly journals The Cyclophilin ROC3 Regulates ABA-Induced Stomatal Closure and the Drought Stress Response of Arabidopsis thaliana

2021 ◽  
Vol 12 ◽  
Author(s):  
Huiping Liu ◽  
Jianlin Shen ◽  
Chao Yuan ◽  
Dongxue Lu ◽  
Biswa R. Acharya ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Drought Stress ◽  
Stress Response ◽  
Stomatal Closure ◽  
Anion Channel ◽  
Crop Productivity ◽  
Drought Response ◽  
Wild Type ◽  
Ros Homeostasis ◽  
Ros Accumulation

Drought causes a major constraint on plant growth, development, and crop productivity. Drought stress enhances the synthesis and mobilization of the phytohormone abscisic acid (ABA). Enhanced cellular levels of ABA promote the production of reactive oxygen species (ROS), which in turn induce anion channel activity in guard cells that consequently leads to stomatal closure. Although Cyclophilins (CYPs) are known to participate in the biotic stress response, their involvement in guard cell ABA signaling and the drought response remains to be established. The Arabidopsis thaliana gene ROC3 encodes a CYP. Arabidopsis roc3 T-DNA mutants showed a reduced level of ABA-activated S-type anion currents, and stomatal closure than wild type (WT). Also, roc3 mutants exhibited rapid loss of water in leaf than wild type. Two complementation lines of roc3 mutants showed similar stomatal response to ABA as observed for WT. Both complementation lines also showed similar water loss as WT by leaf detached assay. Biochemical assay suggested that ROC3 positively regulates ROS accumulation by inhibiting catalase activity. In response to ABA treatment or drought stress, roc3 mutant show down regulation of a number of stress responsive genes. All findings indicate that ROC3 positively regulates ABA-induced stomatal closure and the drought response by regulating ROS homeostasis and the expression of various stress-activated genes.

scholarly journals A Novel DUF569 Gene Is a Positive Regulator of the Drought Stress Response in Arabidopsis

2021 ◽  
Vol 22 (10) ◽  
pp. 5316
Author(s):  
Rizwana Begum Syed Nabi ◽  
Rupesh Tayade ◽  
Adil Hussain ◽  
Arjun Adhikari ◽  
In-Jung Lee ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Drought Stress ◽  
Global Environmental Change ◽  
Loss Of Function ◽  
Wild Type ◽  
Positive Regulator ◽  
Global Environmental ◽  
Acid Accumulation ◽  
Domain Of Unknown Function ◽  
Rosette Leaf

In the last two decades, global environmental change has increased abiotic stress on plants and severely affected crops. For example, drought stress is a serious abiotic stress that rapidly and substantially alters the morphological, physiological, and molecular responses of plants. In Arabidopsis, several drought-responsive genes have been identified; however, the underlying molecular mechanism of drought tolerance in plants remains largely unclear. Here, we report that the “domain of unknown function” novel gene DUF569 (AT1G69890) positively regulates drought stress in Arabidopsis. The Arabidopsis loss-of-function mutant atduf569 showed significant sensitivity to drought stress, i.e., severe wilting at the rosette-leaf stage after water was withheld for 3 days. Importantly, the mutant plant did not recover after rewatering, unlike wild-type (WT) plants. In addition, atduf569 plants showed significantly lower abscisic acid accumulation under optimal and drought-stress conditions, as well as significantly higher electrolyte leakage when compared with WT Col-0 plants. Spectrophotometric analyses also indicated a significantly lower accumulation of polyphenols, flavonoids, carotenoids, and chlorophylls in atduf569 mutant plants. Overall, our results suggest that novel DUF569 is a positive regulator of the response to drought in Arabidopsis.

scholarly journals Increased Abscisic Acid Sensitivity And Drought Stress By Overexpression of Abscisic Acid Receptors In Arabidopsis Thaliana

2021 ◽  
Author(s):  
Li Qing ◽  
Tian Qianqian ◽  
Zhang Yue ◽  
Niu Mengxue ◽  
Yu Xiaoqian ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Drought Stress ◽  
Stress Responses ◽  
Populus Euphratica ◽  
Wild Type ◽  
Positive Role ◽  
Acid Sensitivity ◽  
Cis Acting ◽  
Aba Sensitivity ◽  
Use Efficiency

Abstract Abscisic acid (ABA) is a key plant hormone that regulates plant growth development and stress response. ABA is recognized and bound by ABA Receptor PYR/PYL/RCAR (referred to as PYLs). However, little is known about the PYLs gene family in Populus euphratica. Here, we identified 12 PYLs in P. euphratica and named PePYL1-12. Phylogenetic analysis divided the 12 PePYLs into three subfamilies. Subcellular localization showed that PePYL2, PePYL4, PePYL5, PePYL6, and PePYL9 were located in the cytoplasm and nucleus, PePYL10 localized in the nucleus. The promoter of 12 PePYLs contains hormones- and abiotic stress-related cis-acting elements. Moreover, ABA and drought significantly up-regulation the expression of PePYL6 and PePYL9. To study the performance of PePYLs under ABA and drought stress, we generated transgenic Arabidopsis plants overexpressing PePYL6 and PePYL9. Compared with wild type, transgenic Arabidopsis enhanced ABA sensitivity during seed germination and root growth, improved water use efficiency and drought resistance. Taken together, our results confirmed that PePYL6 and PePYL9 play a positive role in ABA-mediated stress responses in P. euphratica.

scholarly journals DNA methyltransferase CHROMOMETHYLASE3 prevents ONSEN transposon silencing under heat stress

PLoS Genetics ◽  
2021 ◽  
Vol 17 (8) ◽  
pp. e1009710
Author(s):  
Kosuke Nozawa ◽  
Jiani Chen ◽  
Jianjun Jiang ◽  
Sarah M. Leichter ◽  
Masataka Yamada ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Heat Stress ◽  
Dna Methyltransferase ◽  
Dna Methyltransferases ◽  
Genome Integrity ◽  
Loss Of Function ◽  
Wild Type ◽  
Positive Role ◽  
Transposon Silencing

DNA methylation plays crucial roles in transposon silencing and genome integrity. CHROMOMETHYLASE3 (CMT3) is a plant-specific DNA methyltransferase responsible for catalyzing DNA methylation at the CHG (H = A, T, C) context. Here, we identified a positive role of CMT3 in heat-induced activation of retrotransposon ONSEN. We found that the full transcription of ONSEN under heat stress requires CMT3. Interestingly, loss-of-function CMT3 mutation led to increased CHH methylation at ONSEN. The CHH methylation is mediated by CMT2, as evidenced by greatly reduced CHH methylation in cmt2 and cmt2 cmt3 mutants coupled with increased ONSEN transcription. Furthermore, we found more CMT2 binding at ONSEN chromatin in cmt3 compared to wild-type accompanied with an ectopic accumulation of H3K9me2 under heat stress, suggesting a collaborative role of H3K9me2 and CHH methylation in preventing heat-induced ONSEN activation. In summary, this study identifies a non-canonical role of CMT3 in preventing transposon silencing and provides new insights into how DNA methyltransferases regulate transcription under stress conditions.

scholarly journals The LRR-RLK Protein HSL3 Regulates Stomatal Closure and the Drought Stress Response by Modulating Hydrogen Peroxide Homeostasis

2020 ◽  
Vol 11 ◽  
Author(s):  
Xuan-shan Liu ◽  
Chao-chao Liang ◽  
Shu-guo Hou ◽  
Xin Wang ◽  
Dong-hua Chen ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Drought Stress ◽  
Opposite Effect ◽  
Stomatal Closure ◽  
Guard Cells ◽  
Loss Of Function ◽  
Wild Type ◽  
Oxidase Gene ◽  
Moisture Deficit ◽  
Receptor Like Kinase

Guard cells shrink in response to drought stress and abscisic acid (ABA) signaling, thereby reducing stomatal aperture. Hydrogen peroxide (H2O2) is an important signaling molecule acting to induce stomatal closure. As yet, the molecular basis of control over the level of H2O2 in the guard cells remains largely unknown. Here, the leucine-rich repeat (LRR)—receptor-like kinase (RLK) protein HSL3 has been shown to have the ability to negatively regulate stomatal closure by modulating the level of H2O2 in the guard cells. HSL3 was markedly up-regulated by treating plants with either ABA or H2O2, as well as by dehydration. In the loss-of-function hsl3 mutant, both stomatal closure and the activation of anion currents proved to be hypersensitive to ABA treatment, and the mutant was more tolerant than the wild type to moisture deficit; the overexpression of HSL3 had the opposite effect. In the hsl3 mutant, the transcription of NADPH oxidase gene RbohF involved in H2O2 production showed marked up-regulation, as well as the level of catalase activity was weakly inducible by ABA, allowing H2O2 to accumulate in the guard cells. HSL3 was concluded to participate in the regulation of the response to moisture deficit through ABA-induced stomatal closure triggered by the accumulation of H2O2 in the guard cells.

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