scholarly journals A qPCR Assay for the Fast Detection and Quantification of Colletotrichum lupini

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1548
Author(s):  
Tim Kamber ◽  
Nachelli Malpica-López ◽  
Monika M. Messmer ◽  
Thomas Oberhänsli ◽  
Christine Arncken ◽  
...  
Keyword(s):  
Lupinus Albus ◽  
Qpcr Assay ◽  
White Lupin ◽  
Infected Seed ◽  
Legume Crop ◽  
Mother Plants ◽  
Lupin Seeds ◽  
Infection Source ◽  
Colletotrichum Lupini ◽  
Detection And Quantification

White lupin (Lupinus albus) represents an important legume crop in Europe and other parts of the world due to its high protein content and potential for low-input agriculture. However, most cultivars are susceptible to anthracnose caused by Colletotrichum lupini, a seed- and air-borne fungal pathogen that causes severe yield losses. The aim of this work was to develop a C. lupini-specific quantitative real-time TaqMan PCR assay that allows for quick and reliable detection and quantification of the pathogen in infected seed and plant material. Quantification of C. lupini DNA in dry seeds allowed us to distinguish infected and certified (non-infected) seed batches with DNA loads corresponding to the disease score index and yield of the mother plants. Additionally, C. lupini DNA could be detected in infected lupin shoots and close to the infection site, thereby allowing us to study the disease cycle of this hemibiotrophic pathogen. This qPCR assay provides a useful diagnostic tool to determine anthracnose infection levels of white lupin seeds and will facilitate the use of seed health assessments as a strategy to reduce the primary infection source and spread of this disease.

scholarly journals White lupin (Lupinus albus L.) – nutritional and health values in human nutrition – a review

2017 ◽  
Vol 35 (No. 2) ◽  
pp. 95-105 ◽  
Author(s):  
Prusinski Janusz
Keyword(s):  
Lupinus Albus ◽  
Human Nutrition ◽  
White Lupin ◽  
Small Scale ◽  
Gluten Free ◽  
Omega 3 ◽  
Health Values ◽  
Healthy Diets ◽  
Omega 6 ◽  
Lupin Seeds

White lupin seeds have been used in human nutrition and treatment for several thousand years. Nowadays the use of white lupin seeds is limited by a small scale of their production. However, in the last 20 years quite new properties of white lupin have been discovered for the application in the production of different kinds of functional food. Unique traits of protein, fatty acids with a desirable ratio of omega-6 to omega-3 acids, and fibre as well as other specific components, for example oligosaccharides and antioxidants or non-starch carbohydrates, make white lupin an excellent component in many healthy diets. The effects of white lupin components concern the physiological condition of the human body, including diabetes, hypertension, obesity, cardiovascular diseases, lipid concentration, glycaemia, appetite, insulin resistance, and colorectal cancer. Seeds are used among others for the production of gluten-free flour, bacterial and fungal fermented products, noodle and pasta products, as substitutes of meat, egg protein and sausages, also are cooked, roasted and ground and mixed with cereal flour in the production of bread, crisps and pasta, crisps and dietary dishes.

scholarly journals Quantification of Biologically Fixed Nitrogen by White Lupin (Lupins albus L.) and Its Subsequent Uptake by Winter Wheat Using the 15N Isotope Dilution Method

Agronomy ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1392
Author(s):  
Stanisław Kalembasa ◽  
Jerzy Szukała ◽  
Agnieszka Faligowska ◽  
Dorota Kalembasa ◽  
Barbara Symanowicz ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Isotope Dilution ◽  
Crop Residues ◽  
Lupinus Albus ◽  
N Fertilization ◽  
White Lupin ◽  
Isotope Dilution Method ◽  
Dilution Method ◽  
First Year ◽  
Lupin Seeds

A field experiment was carried out in 2016–2018 in a white lupin (Lupinus albus L.)-winter wheat (Triticum aestivum cv. ‘Bogatka’) crop rotation. The aim of this study was to determine the amount of nitrogen (N) that was biologically fixed by the white lupin crop in the first year of the rotation and to estimate how much of this N was then taken up from the lupin residues by winter wheat in the second and third years of the rotation. Biologically fixed N was determined by the isotope-dilution method (ID15N) by applying 30 kg N ha−1 of 15N-labeled fertilizer (15NH4)2SO4 (containing 20.1 at.% 15N) to the white lupin and the reference plant spring wheat. The yields of white lupin seeds and crop residues were 3.92 t ha−1 and 4.30 t ha−1, respectively. The total amount of N in the white lupin biomass was 243.2 kg ha−1, which included 209.3 kg ha−1 in the seeds and 33.9 kg ha−1 in the residues. The 15N-labeled residue of white lupin was cut and ploughed into soil. Our results indicate that 111.2 kg N ha−1 was fixed from the atmosphere by the lupin plants, with 93.7 kg ha−1 found in the seeds and 17.5 kg ha−1 in the residues. In the second and third years of the rotation when winter wheat was cultivated, the plots were divided into two groups of subplots (1) without N-fertilization (control) and (2) with an application of 100 kg N ha−1. In the first year of winter wheat cultivation, 20.0% and 21.0% of N from the crop residues was taken up by the control and N-fertilization plots, respectively, while in the second year, uptake was lower at 7.12% and 9.27% in the control and N-fertilized plots, respectively.

scholarly journals Effect of extruding on ruminal disappearance and lower gastrointestinal tract digestion of white lupin seeds

1993 ◽  
Vol 73 (3) ◽  
pp. 571-579 ◽  
Author(s):  
A. R. Kibelolaud ◽  
M. Vernay ◽  
C. Bayourthe ◽  
R. Moncoulon
Keyword(s):  
Gastrointestinal Tract ◽  
Crude Protein ◽  
Lupinus Albus ◽  
Neutral Detergent Fiber ◽  
White Lupin ◽  
Lower Gastrointestinal Tract ◽  
Rumen Degradation ◽  
Outflow Rate ◽  
Lupin Seeds

The effect of extruding white lupin (Lupinus albus 'Lublanc') seeds (WLS) at 110, 130, 150 or 180 °C on the in vitro solubility of crude protein (CP) and in sacco rumen degradation and intestinal digestion of rumen escape CP and fiber was determined. Rumen degradation was estimated by incubating nylon bags in the rumen of cows for 2, 4, 8, 16, 24 and 48 h. Extruding WLS at 110, 130, 150 and 180 °C reduced the CP-solubility by 32.8, 47.7, 58.4 and 67.5%, respectively. The effective ruminal degradabilities of CP, acid detergent fiber (ADF) and neutral detergent fiber (NDF) were evaluated assuming a ruminal outflow rate of 0.06 h−1. Heating WLS at 110, 130, 150 and 180 °C decreased the ruminal degradability of CP value: 89.8, 79.9, 65.1, 61.8 vs. 93.4% (raw), respectively; the corresponding values for ADF and NDF were: 38.4, 35.3, 34.2, 27.6 vs. 43.6% (raw) and 37.7, 33.1, 32.2, 26.5 vs. 39.4% (raw). Postruminal digestion was estimated using a sequence of ruminal in situ incubation for 4, 8 and 16 h, in vitro incubation in an acid-pepsin bath for 3 h and a mobile nylon bag technique distal to the abomasum. Extrusion of WLS decreased the degradability of CP, ADF and NDF in the rumen with a corresponding increase in the amounts digested in the postruminal sections; the whole-tract digestibility was generally unchanged. Therefore, the processing shifted the digestion of these components from rumen to the lower gastrointestinal tract. Key words: White lupin seed, in vitro solubility, rumen degradability, postruminal digestion, crude protein, detergent fiber

Overcoming the barriers of combining early flowering and anthracnose resistance in white lupin (Lupinus albus L.) for the Northern Agricultural Region of Western Australia

2013 ◽  
Vol 64 (9) ◽  
pp. 914 ◽  
Author(s):  
Kedar Nath Adhikari ◽  
Geoff Thomas ◽  
Dean Diepeveen ◽  
Richard Trethowan
Keyword(s):  
Western Australia ◽  
Lupinus Albus ◽  
Early Flowering ◽  
White Lupin ◽  
Grain Legume ◽  
New Variety ◽  
Agricultural Region ◽  
Anthracnose Resistance ◽  
Legume Crop ◽  
Lupinus Albus L

White lupin (Lupinus albus L.) is an important grain legume crop in Australia. The anthracnose incursion in the mid-1990s wiped out the white lupin industry in Western Australia (WA). Since then, incorporation of anthracnose resistance has been a major focus in white lupin breeding. After a series of experiments and targeted breeding in WA, high-yielding anthracnose-resistant genotypes were developed. One of these lines, Amira, was released in 2012 as a replacement for the then-benchmark variety Andromeda. Amira is high-yielding and early-maturing and it has substantially improved resistance to anthracnose compared with Andromeda. Its yield and grain quality are similar to Kiev Mutant and it will be suitable for growing in parts of the Northern Agricultural Region of WA where anthracnose risk is moderate to low. With the adoption of this new variety, reliable production of white lupin can recommence in WA. The growing season in WA is characterised by terminal drought, and early flowering is as important as anthracnose resistance. However, combining these traits was difficult and their combination was not achieved at a desired level in earlier work. The incorporation of the early-flowering trait from a different genetic source from France demonstrated that it is possible to combine these traits at an appropriate level. There was no genetic linkage between the two traits, and consequently, new genotypes with earlier phenology and higher levels of resistance than Amira were developed. The combination of early flowering and anthracnose resistance represents a breakthrough that will significantly improve the adaptation and profitability of white lupin production in WA.

Some chemical properties of white lupin seeds (Lupinus albus L.)

2005 ◽  
Vol 89 (3) ◽  
pp. 341-345 ◽  
Author(s):  
M. Erbaş ◽  
M. Certel ◽  
M.K. Uslu
Keyword(s):  
Lupinus Albus ◽  
Chemical Properties ◽  
White Lupin ◽  
Lupin Seeds ◽  
Lupinus Albus L

scholarly journals WHITE LUPIN SEEDS YIELD (LUPINUS ALBUS L.) DEPENDING ON HERBICIDAL EFFECT

2019 ◽  
pp. 48-53
Author(s):  
Оksana Veresenko ◽  
◽  
Serhii Poltoretskyi ◽  
Nataliya Poltoretska ◽  
Lidiia Kononenko ◽  
...  
Keyword(s):  
Lupinus Albus ◽  
White Lupin ◽  
Lupin Seeds ◽  
Lupinus Albus L

scholarly journals (41) Value-added Products from Agriculture: Sprouts from Canola and Lupin Seeds

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1029D-1029
Author(s):  
Harbans Bhardwaj ◽  
Anwar Hamama
Keyword(s):  
Lupinus Albus ◽  
Value Added ◽  
Dry Weight ◽  
The United States ◽  
Weight Basis ◽  
White Lupin ◽  
Human Consumption ◽  
Atlantic Region ◽  
Lupin Seeds ◽  
Value Added Products

Sprouts from various seeds are considered healthy for human consumption. However, no information is available about sprouts made from canola (Brassica napus L.) and white lupin (Lupinus albus L.), two new potential alternate crops in the mid-Atlantic region of the United States. Canola sprouts, on an average, contained (g/100 g, dry weight basis) 38.9 oil, and 61.5, 18.6, 9.2, 7.4, 92.6, 64.8, and 27.8 percent of 18:1, 18:2, 18:3, total unsaturated, total saturated, MUFA, and PUFA fatty acids, respectively, in the oil. Corresponding values for white lupin sprouts were: 6.5, 43.0, 24.9, 9.3, 17.9, 82.1, 47.9, and 34.2. Canola sprouts contained 26.9% protein, whereas white lupin sprouts contained 26.3% protein. Details of these experiments and further results would be presented.

scholarly journals Complete Genome Sequence of the plant pathogenic fungus Colletotrichum lupini.

2021 ◽  
Author(s):  
Riccardo Baroncelli ◽  
Flora Pensec ◽  
Daniele Da Lio ◽  
Thaís Regina Boufleur ◽  
Isabel Vicente ◽  
...  
Keyword(s):  
Plant Pathogens ◽  
Pathogenic Fungus ◽  
Lupinus Albus ◽  
Genome Structure ◽  
White Lupin ◽  
Sequencing Technologies ◽  
Wide Range ◽  
Protein Encoding ◽  
Colletotrichum Lupini ◽  
Encoding Genes

Colletotrichum is a fungal genus (Ascomycota, Sordariomycetes, Glomerellaceae) that includes many economically important plant pathogens that cause devastating diseases of a wide range of plants. In this work, using a combination of long and short-read sequencing technologies, we sequenced the genome of Colletotrichum lupini RB221, isolated from white lupin (Lupinus albus) in France during a survey in 2014. The genome was assembled into eleven nuclear chromosomes and a mitochondrial genome with a total assembly size of 63.41Mb and 36.55 Kb, respectively. A total of 18324 protein encoding genes have been predicted, of which only 39 are specific to C. lupini. To the best of our knowledge this is the first genome of this species to be fully sequenced and to get publicly released. This resource will provide insight into pathogenicity factors and will help to get a better understanding of the evolution and genome structure of this important plant pathogen.

scholarly journals Development of PCR-based markers and whole-genome selection model for anthracnose resistance in white lupin (Lupinus albus L.)

2020 ◽  
Vol 61 (4) ◽  
pp. 531-545
Author(s):  
Sandra Rychel-Bielska ◽  
Nelson Nazzicari ◽  
Piotr Plewiński ◽  
Wojciech Bielski ◽  
Paolo Annicchiarico ◽  
...  
Keyword(s):  
Genomic Selection ◽  
Lupinus Albus ◽  
Predictive Ability ◽  
Genotyping By Sequencing ◽  
High Specificity ◽  
White Lupin ◽  
Grain Legume ◽  
Anthracnose Resistance ◽  
Legume Crop ◽  
Molecular Tracking

Abstract White lupin (Lupinus albus L.) is a high-protein grain legume crop, grown since ancient Greece and Rome. Despite long domestication history, its cultivation remains limited, partly because of susceptibility to anthracnose. Only some late-flowering, bitter, low-yielding landraces from Ethiopian mountains displayed resistance to this devastating disease. The resistance is controlled by various genes, thereby complicating the breeding efforts. The objective of this study was developing tools for molecular tracking of Ethiopian resistance genes based on genotyping-by-sequencing (GBS) data, envisaging linkage mapping and genomic selection approaches. Twenty GBS markers from two major quantitative trait loci (QTLs), antr04_1/antr05_1 and antr04_2/antr05_2, were converted to PCR-based markers using assigned transcriptome sequences. Newly developed markers improved mapping resolution around both anthracnose resistance loci, providing more precise QTL estimation. PCR-based screening of diversified domesticated and primitive germplasm revealed the high specificity of two markers for the antr04_1/antr05_1 locus (TP222136 and TP47110) and one for the antr04_2/antr05_2 locus (TP338761), highlighted by simple matching coefficients of 0.96 and 0.89, respectively. Moreover, a genomic selection approach based on GBS data of a recombinant inbred line mapping population was assessed, providing an average predictive ability of 0.56. These tools can be used for preselection of candidate white lupin germplasm for anthracnose resistance assays.

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