scholarly journals The Role of the GSTF11 Gene in Resistance to Powdery Mildew Infection and Cold Stress

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2729
Author(s):  
Elena Mikhaylova ◽  
Emil Khusnutdinov ◽  
Michael Yu Shein ◽  
Valentin Yu Alekseev ◽  
Yuri Nikonorov ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Powdery Mildew ◽  
Cold Stress ◽  
Temperate Climate ◽  
Glutathione S Transferase ◽  
Mycelium Growth ◽  
Mrna Content ◽  
Response To Stress ◽  
The Impact ◽  
Gst Activity

Oilseed rape (Brassica napus) is an economically important crop. In a temperate climate, powdery mildew Erysiphe crucifertaum can drastically reduce its yield. Nevertheless, cultivars resistant to this fungal disease have not yet been selected. Glutathione S-transferase GSTF11 is involved in glucosinolate (GSL) biosynthesis and response to stress, including fungal deceases. However, the impact of exogenous GSTF11 gene expression on resistance to powdery mildew has not yet been confirmed and requires further investigation. Transgenic B. napus was generated for this purpose. It demonstrated increased GST activity and a higher GSH:GSSG ratio under normal conditions. Powdery mildew Erysiphe crucifertaum caused 50% mortality in wild type (WT) plants. In most of transgenic plants, mycelium growth was inhibited. The infection contributed to higher GSTF11 expression and increased levels of glutathione (GSH) and oxidized glutathione (GSSG) in both transgenic and WT plants. In contrast, GSTF11 mRNA content, GST activity and GSSG level were lower only in WT plants. In transgenic plants, increased resistance to powdery mildew correlated with a lower GSH:GSSG ratio, indicating a higher content of neutralized toxic molecules. GSTF11 expression was also affected by cold stress, but not drought. At −1 °C, the expression level increased only in transgenic plants. Therefore, GSTF11 appears to be nonspecific and is able to protect plants under several types of stress. This gene could be used as a target in the production of stress tolerant cultivars.

Chemoprotective Effects of Propolis on Aflatoxin B1-Induced Hepatotoxicity in Rats: Oxidative Damage and Hepatotoxicity by Modulating TP53, Oxidative Stress

2020 ◽  
Vol 17 (3) ◽  
pp. 191-199
Author(s):  
Seval Yilmaz ◽  
Fatih Mehmet Kandemir ◽  
Emre Kaya ◽  
Mustafa Ozkaraca
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Oxidative Damage ◽  
Aflatoxin B1 ◽  
Tp53 Gene ◽  
Control Group ◽  
Glutathione S Transferase ◽  
Gene Expressions ◽  
Cat Gene ◽  
Gst Activity

Objective: This study aimed to detect hepatic oxidative damage caused by aflatoxin B1 (AFB1), as well as to examine how propolis protects against hepatotoxic effects of AFB1. Method: Rats were split into four groups as control group, AFB1 group, propolis group, AFB1+ propolis group. Results: There was significant increase in malondialdehyde (MDA) level and tumor suppressor protein (TP53) gene expression, Glutathione (GSH) level, Catalase (CAT) activity, CAT gene expression decreased in AFB1 group in blood. MDA level and Glutathione-S-Transferase (GST) activity, GST and TP53 gene expressions increased in AFB1 group, whereas GSH level and CAT activity alongside CAT gene expression decreased in liver. AFB1+propolis group showed significant decrease in MDA level, GST activity, TP53 and GST gene expressions, GSH level and CAT activity and CAT gene expression increased in liver compared to AFB1 group. Conclusion: These results suggest that propolis may potentially be natural agent that prevents AFB1- induced oxidative stress and hepatotoxicity.

scholarly journals Impact of crop residue management on crop production and soil chemistry after seven years of crop rotation in temperate climate, loamy soils

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4836 ◽  
Author(s):  
Marie-Pierre Hiel ◽  
Sophie Barbieux ◽  
Jérôme Pierreux ◽  
Claire Olivier ◽  
Guillaume Lobet ◽  
...  
Keyword(s):  
Organic Carbon ◽  
Crop Production ◽  
Crop Residue ◽  
Crop Residues ◽  
Conventional Tillage ◽  
Residue Management ◽  
Temperate Climate ◽  
Reduced Tillage ◽  
Crop Residue Management ◽  
The Impact

Society is increasingly demanding a more sustainable management of agro-ecosystems in a context of climate change and an ever growing global population. The fate of crop residues is one of the important management aspects under debate, since it represents an unneglectable quantity of organic matter which can be kept in or removed from the agro-ecosystem. The topic of residue management is not new, but the need for global conclusion on the impact of crop residue management on the agro-ecosystem linked to local pedo-climatic conditions has become apparent with an increasing amount of studies showing a diversity of conclusions. This study specifically focusses on temperate climate and loamy soil using a seven-year data set. Between 2008 and 2016, we compared four contrasting residue management strategies differing in the amount of crop residues returned to the soil (incorporation vs. exportation of residues) and in the type of tillage (reduced tillage (10 cm depth) vs. conventional tillage (ploughing at 25 cm depth)) in a field experiment. We assessed the impact of the crop residue management on crop production (three crops—winter wheat, faba bean and maize—cultivated over six cropping seasons), soil organic carbon content, nitrate (${\mathrm{NO}}_{3}^{-}$), phosphorus (P) and potassium (K) soil content and uptake by the crops. The main differences came primarily from the tillage practice and less from the restitution or removal of residues. All years and crops combined, conventional tillage resulted in a yield advantage of 3.4% as compared to reduced tillage, which can be partly explained by a lower germination rate observed under reduced tillage, especially during drier years. On average, only small differences were observed for total organic carbon (TOC) content of the soil, but reduced tillage resulted in a very clear stratification of TOC and also of P and K content as compared to conventional tillage. We observed no effect of residue management on the ${\mathrm{NO}}_{3}^{-}$ content, since the effect of fertilization dominated the effect of residue management. To confirm the results and enhance early tendencies, we believe that the experiment should be followed up in the future to observe whether more consistent changes in the whole agro-ecosystem functioning are present on the long term when managing residues with contrasted strategies.

scholarly journals Outer wall with thermal barrier. Impact of the barrier on heat losses and CO2 emissions

2020 ◽  
Vol 29 (2) ◽  
pp. 223-233
Author(s):  
Ewa Figiel ◽  
Dorota Leciej-Pirczewska
Keyword(s):  
Meteorological Data ◽  
Renewable Energy Sources ◽  
Outer Wall ◽  
Energy Performance ◽  
Temperate Climate ◽  
Thermal Barrier ◽  
Single Family ◽  
Heating And Cooling ◽  
The Impact ◽  
Supply Systems

New demands for lowering energy consumption of buildings lead to many new solutions including, amongst others, the introduction of an outer wall thermal barrier for both heating and cooling effect. The analysed thermo-active-wall-barrier is a water-based system, where the pipes are embedded in the wall construction. It enables the use of a low-temperature barrier medium for space heating, thereby increasing the efficiency of all potential energy supply systems using renewable energy sources. The pipes form an active thermal barrier for heat transfer between the outer and the heated space. There are many possibilities to place the pipes in the wall for example in the case of energetic thermo-modernisation. Our research and calculations have shown that thermo-active-wall-barrier is sensitive to the location of pipes. The following paper also provides a study of the impact of thermal barrier on a building’s energy performance. The analysis was conducted for a single-family house in a temperate climate based on parameters taken from one of the Polish meteorological data-bases. Calculations using current procedure of evaluating building energy performance show, that the thermal barrier can contribute to signifi cant reduction of transmission energy loss thus lowering the environmental impact.

scholarly journals The impact of the COVID-19 pandemic on mental health

2021 ◽  
Vol 11 (8) ◽  
pp. 44-54
Author(s):  
Julia Budzyńska ◽  
Magdalena Leśniewska ◽  
Ilona Kozioł ◽  
Joanna Milanowska
Keyword(s):  
Mental Health ◽  
Psychological Impact ◽  
Economic Security ◽  
Post Traumatic Stress ◽  
Past Infection ◽  
Global Epidemic ◽  
Response To Stress ◽  
Post Traumatic ◽  
Health Organization ◽  
The Impact

BACKGROUND: The outbreak of a coronavirus pandemic in 2019 posed a serious threat to the global population. Coronavirus Disease 2019 (COVID-19) has not only been a public health emergency, but has also affected mental health worldwide. Patients were exposed to a number of significant stressors during COVID-19 infection by which it appears that it may have had a major impact on mental health. Little is known about changes in levels of psychological affect, stress, anxiety and depression during this pandemic.AIM OF THE STUDY: The purpose of this study was to analyze the most recent available literature on the association of pandemic COVID-19 with psychiatric disorders occurring both as a result of past infection and in response to stress associated with the new situation of a global epidemic of Sars-CoV-2 virus.RESULTS: The psychological effects of the epidemic are clearly noticeable. The study identified groups particularly vulnerable to developing symptoms of anxiety, depression and post-traumatic stress disorder. Risk factors were also identified in people working in health care as having direct contact with the effects of infection with the new virus.SUMMARY AND CONCLUSIONS: Key elements of preventing psychological impact are the creation of a mental health organization. Another important task for governments is to create the conditions for economic security, as financial problems or job losses can also be the cause of mental crises.

scholarly journals The RhoA-ROCK pathway in the regulation of T and B cell responses

F1000Research ◽  
2016 ◽  
Vol 5 ◽  
pp. 2295 ◽  
Author(s):  
Edd Ricker ◽  
Luvana Chowdhury ◽  
Woelsung Yi ◽  
Alessandra B. Pernis
Keyword(s):  
Immune Responses ◽  
Biological Processes ◽  
Dynamic Interactions ◽  
Cell Responses ◽  
Important Mediator ◽  
Downstream Effectors ◽  
Wide Range ◽  
Response To Stress ◽  
The Impact ◽  
Rho Kinases

Effective immune responses require the precise regulation of dynamic interactions between hematopoietic and non-hematopoietic cells. The Rho subfamily of GTPases, which includes RhoA, is rapidly activated downstream of a diverse array of biochemical and biomechanical signals, and is emerging as an important mediator of this cross-talk. Key downstream effectors of RhoA are the Rho kinases, or ROCKs. The ROCKs are two serine-threonine kinases that can act as global coordinators of a tissue’s response to stress and injury because of their ability to regulate a wide range of biological processes. Although the RhoA-ROCK pathway has been extensively investigated in the non-hematopoietic compartment, its role in the immune system is just now becoming appreciated. In this commentary, we provide a brief overview of recent findings that highlight the contribution of this pathway to lymphocyte development and activation, and the impact that dysregulation in the activation of RhoA and/or the ROCKs may exert on a growing list of autoimmune and lymphoproliferative disorders.

scholarly journals Impact of chronic stress on periodontal health

2018 ◽  
Vol 24 (1) ◽  
pp. 44-50 ◽  
Author(s):  
Mathieu Gunepin ◽  
Florence Derache ◽  
Marion Trousselard ◽  
Bruno Salsou ◽  
Jean-Jacques Risso
Keyword(s):  
Periodontal Disease ◽  
Chronic Stress ◽  
Negative Impact ◽  
Periodontal Diseases ◽  
Behavioral Changes ◽  
Educational Objective ◽  
Pathogenic Microorganisms ◽  
Periodontal Health ◽  
Response To Stress ◽  
The Impact

Introduction: Periodontal diseases are caused by pathogenic microorganisms that induce increases in of local and systemic proinflammatory cytokines, resulting in periodontal damage. The onset and evolution of periodontal diseases are influenced by many local and systemic risk factors. Educational objective: In this article, we aim to review the results of the research on the impact of chronic stress on the occurrence, development, and response to periodontal disease treatments and on the pathophysiological mechanisms of periodontal disease. Conclusion: Chronic stress has a negative impact on the occurrence, development, and response to the treatment of periodontal disease via indirect actions on the periodontium. This can result from behavioral changes caused by stress (poor dental hygiene, smoking, etc.) and a direct neuroimmunoendocrinological action related to the consequences (particularly immunological) of the secretion of certain chemicals (e.g., cortisol) induced by the activation of the hypothalamus and the autonomic nervous system in response to stress. These factors necessitate multidisciplinary management (e.g., physician, oral surgeon, and psychologist) of patients to identify subjects with chronic stress and to employ countermeasures to decrease the impact of stress on the periodontium.

scholarly journals Influence of Agaricus Bisporus Establishment and Fungicidal Treatments on Casing Soil Metataxonomy During Mushroom Cultivation

2021 ◽  
Author(s):  
Maria Luisa Tello ◽  
Rebeca Lavega ◽  
Margarita Pérez ◽  
Antonio J. Pérez ◽  
Michael Thon ◽  
...  
Keyword(s):  
Agaricus Bisporus ◽  
Illumina Miseq ◽  
Biological Efficiency ◽  
Its2 Region ◽  
Rrna Gene ◽  
Mushroom Cultivation ◽  
Microbial Composition ◽  
Mycelium Growth ◽  
Bacterial Phyla ◽  
The Impact

Abstract The cultivation of edible mushroom is an emerging sector with a potential yet to be discovered. Unlike plants, it is a less developed agriculture where many studies are lacking to optimize the cultivation. Mushrooms are a source of resources still to be revealed, which have applications not only in food, but in many other sectors such as health, industry and biotechnology. Mushroom cultivation consists of the development of selective substrates through composting where the mushroom grows via solid fermentation process. In case of Agaricus bisporus, the compost fully colonized by mycelium hardly produces mushrooms and it is necessary to apply a casing layer with certain physical, chemical and biological characteristics to shift from the vegetative mycelium to the reproductive one, where the native microbiota plays crucial roles. Currently, the industry faces a challenge to substitute the actual peat based casing materials due to the limited natural resources and the impact on the peatlands where peat is extracted.In this work we have employed high-throughput techniques by next generation sequencing to screen the microbial structure of casing soil employed in mushroom cultivation while sequencing V3-V4 of the 16S rRNA gene for bacteria and the ITS2 region of rRNA for fungi in an Illumina MiSeq. In addition, the microbiome dynamics and evolution (bacterial and fungal communities) in peat based casing along the process of incubation of Agaricus bisporus have been studied, while comparing the effect of fungicidal treatment (Chlorothalonil and Metrafenone). Statistically significant changes in populations of bacteria and fungi were observed. Microbial composition differed significantly based on incubation day, changing radically from the original communities to a specific microbial composition adapted to enhance the A. bisporus mycelium growth. Chlorothalonil treatment seems to delay casing colonization by A. bisporus. Proteobacteria and Bacteroidota appeared as the most dominant bacterial phyla. We observed a great change in the structure of the bacteria populations between day 0 and the following days. Fungi populations changed more gradually, A. bisporus displacing the rest of the species as the cultivation cycle progresses. A better understanding of the microbial communities in the casing will hopefully allow us to increase the biological efficiency during production as well as possibly help us to have a clearer view of the microbial community-pathogen relationships as they are directly related to disease development.

scholarly journals Role of ovarian sympathetic nerves and cholinergic local system during cold stress

2019 ◽  
Vol 242 (2) ◽  
pp. 115-124 ◽  
Author(s):  
Raul Riquelme ◽  
Freddy Ruz ◽  
Artur Mayerhofer ◽  
Hernán E Lara
Keyword(s):  
Cold Stress ◽  
Polycystic Ovary ◽  
Recovery Period ◽  
Sympathetic Nerves ◽  
Plasma Testosterone ◽  
Corpora Lutea ◽  
Estrous Cyclicity ◽  
Rat Ovary ◽  
The Impact ◽  
Second Period

An increase in the sympathetic tone in the rat ovary induces a polycystic ovary (PCOS-like) phenotype. No information exists about its impact on fertility. In contrast, increased follicular development and improved fertility in rats were found after pharmacological inhibition of acetylcholinesterase, which increased intraovarian acetylcholine (ACh). Now, we studied the impact of sympathetic stress, followed by a recovery period without stress, on the cholinergic and noradrenergic systems of the rat ovary and on fertility. To activate ovarian sympathetic nerves, female Sprague–Dawley rats were exposed to cold stress (4°C/3 h day for 28 days; first period), followed by a 28-day period without cold stress (second period). No changes in estrous cyclicity during the first period was found. At the end of this period, ovarian levels of NA and ACh were increased. Morphometric analysis showed lower numbers of secondary and antral follicles, enhanced follicular atresia and fewer corpora lutea. Plasma progesterone was lower and testosterone was higher than that in controls. At end of the second period, ovarian ACh levels had returned to control levels, but NA levels remained elevated. The second period was also characterized by the presence of cystic follicles in the ovary, by elevated plasma testosterone and estradiol levels, while progesterone levels were decreased. Estrous cyclicity and ovulation during that period were irregular and fertility decreased. Thus, cold stress initially activated both ovarian noradrenergic and cholinergic system. After stress, the ovary did not fully recover and activation of the noradrenergic system persisted and correlated with cystic ovarian morphology and decreased fertility.

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