scholarly journals The Progression of Xylem Vessel Cell Differentiation is Dependent on the Activity Level of VND7 in Arabidopsis thaliana

Plants ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 39 ◽  
Author(s):  
Risaku Hirai ◽  
Takumi Higaki ◽  
Yuto Takenaka ◽  
Yuki Sakamoto ◽  
Junko Hasegawa ◽  
...  

Xylem vessels are important for water conduction in vascular plants. The VASCULAR-RELATED NAC-DOMAIN (VND) family proteins, master regulators of xylem vessel cell differentiation in Arabidopsis thaliana, can upregulate a set of genes required for xylem vessel cell differentiation, including those involved in secondary cell wall (SCW) formation and programmed cell death (PCD); however, it is not fully understood how VND activity levels influence these processes. Here, we examined the Arabidopsis VND7-VP16-GR line, in which VND7 activity is post-translationally activated by treatments with different concentrations of dexamethasone (DEX), a synthetic glucocorticoid. Our observations showed that 1 nM DEX induced weak SCW deposition, but not PCD, whereas 10 or 100 nM DEX induced both SCW deposition and PCD. The decreased chlorophyll contents and SCW deposition were apparent after 24 h of 100 nM DEX treatment, but became evident only after 48 h of 10 nM DEX treatment. Moreover, the lower DEX concentrations delayed the upregulation of VND7 downstream genes, and decreased their induction levels. They collectively suggest that the regulation of VND activity is important not only to initiate xylem vessel cell differentiation, but also regulate the quality of the xylem vessels through VND-activity-dependent upregulation of the PCD- and SCW-related genes.

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Laura E. Thorp ◽  
Diego Orozco ◽  
Joel A. Block ◽  
Dale R. Sumner ◽  
Markus A. Wimmer

This work evaluated activity levels in a group of healthy older adults to establish a target activity level for adults of similar age after total joint arthroplasty (TJA). With the decreasing age of TJA patients, it is essential to have a reference for activity level in younger patients as activity level affects quality of life and implant design. 54 asymptomatic, healthy older adults with no clinical evidence of lower extremity OA participated. The main outcome measure, average daily step count, was measured using an accelerometer-based activity monitor. On average the group took steps per day, approximately 4000 more steps per day than has been previously reported in patients following total joint arthroplasty. The present work provides a reference for activity after joint arthroplasty which is relevant given the projected number of people under the age of 65 who will undergo joint arthroplasty in the coming years.


2019 ◽  
Vol 40 (6) ◽  
pp. 704-716 ◽  
Author(s):  
Nobuhiro Akiyoshi ◽  
Yoshimi Nakano ◽  
Ryosuke Sano ◽  
Yusuke Kunigita ◽  
Misato Ohtani ◽  
...  

Abstract Vascular plants have two types of water-conducting cells, xylem vessel cells (in angiosperms) and tracheid cells (in ferns and gymnosperms). These cells are commonly characterized by secondary cell wall (SCW) formation and programmed cell death (PCD), which increase the efficiency of water conduction. The differentiation of xylem vessel cells is regulated by a set of NAC (NAM, ATAF1/2 and CUC2) transcription factors, called the VASCULAR-RELATED NAC-DOMAIN (VND) family, in Arabidopsis thaliana Linne. The VNDs regulate the transcriptional induction of genes required for SCW formation and PCD. However, information on the transcriptional regulation of tracheid cell differentiation is still limited. Here, we performed functional analysis of loblolly pine (Pinus taeda Linne) VND homologs (PtaVNS, for VND, NST/SND, SMB-related protein). We identified five PtaVNS genes in the loblolly pine genome, and four of these PtaVNS genes were highly expressed in tissues with tracheid cells, such as shoot apices and developing xylem. Transient overexpression of PtaVNS genes induced xylem vessel cell-like patterning of SCW deposition in tobacco (Nicotiana benthamiana Domin) leaves, and up-regulated the promoter activities of loblolly pine genes homologous to SCW-related MYB transcription factor genes and cellulose synthase genes, as well as to cysteine protease genes for PCD. Collectively, our data indicated that PtaVNS proteins possess transcriptional activity to induce the molecular programs required for tracheid formation, i.e., SCW formation and PCD. Moreover, these findings suggest that the VNS–MYB-based transcriptional network regulating water-conducting cell differentiation in angiosperm and moss plants is conserved in gymnosperms.


Author(s):  
Bruno Leandro de Melo BARRETO ◽  
Jones Silva LIMA ◽  
Diogo Barbosa de ALBUQUERQUE ◽  
Flavio KREIMER ◽  
Álvaro Antonio Bandeira FERRAZ ◽  
...  

ABSTRACT Background: Physical activity enhances quality of life and body image in obese. Behavioural changes are useful tools to increase life conditions of this population. Aim: To evaluate the physical activity level of candidates to bariatric surgery and its relation with quality of life and body image, when patients are encouraged weekly by personal trainers. ) Method: This is a prospective, interventional and longitudinal study with quantitative analysis. Patients were divided into two groups, control (n=28) and interventional (n= 10). Both groups received physical activity and nutritional recommendations and psychological support. Were used the SF36 and Body Shape questionnaires to assess physical activity level and body image and pedometers to count weekly steps. Patients were followed during 12 weeks. ) Results: Were found significant difference in the domains physical activity (p=0.019), pain (p=0.0001) and health general status (p=0.021). No significant difference in body weight (p=0.095) was noted. Conclusion: When assisted by personal trainers, obese patients can change behavior, increase health quality and physical activity levels and experience less pain. Increase in physical activity, when well structured can benefit these patients.


2021 ◽  
pp. 026921552110241
Author(s):  
Ulric S Abonie ◽  
John Saxton ◽  
Katherine Baker ◽  
Florentina J Hettinga

Objective: To examine the association between self-reported activity pacing (a strategy to manage fatigue symptoms) and objectively-measured physical activity behaviours in adults with multiple sclerosis. Design: Single cross-sectional study Setting: Multiple sclerosis rehabilitation centre in Colchester, United Kingdom. Subjects: Twenty-one adults (59 ± 9 years) with multiple sclerosis. Main measures: Physical activity behaviours (activity level: activity counts per minute; activity variability: highest activity counts per minute each day divided by activity counts per minute on that day) were measured with accelerometers. Self-reported activity pacing (Activity Pacing and Risk of Overactivity Questionnaire), fatigue severity (Fatigue Severity Scale) and health-related quality of life (RAND-12-Item Short-Form Health Survey) were measured. Scatter plots were used to explore associations between measures. Results: Activity level was 258 ± 133 counts per minutes, activity variability was 4 ± 1, self-reported activity pacing was 3 ± 1, fatigue severity was 5 ± 2 and health-related quality of life was 43 ± 8. Increased self-reported activity pacing was associated with lower activity levels and less variability in daily activities. Conclusion: This investigation suggests that people with multiple sclerosis who have low physical activity levels could be inappropriately using activity pacing as a reactionary response to their multiple sclerosis symptoms.


Author(s):  
Larissa Monteiro Costa Pereira ◽  
Felipe J. Aidar ◽  
Dihogo Gama de Matos ◽  
Jader Pereira de Farias Neto ◽  
Raphael Fabrício de Souza ◽  
...  

Obesity is a highly prevalent chronic metabolic disease, with an increasing incidence, and is currently approaching epidemic proportions in developing countries. Ouraim was to evaluate the activity levels, quality of life (QoL), clinical parameters, laboratory parameters, and cardiometabolic risk factors afterbariatric surgery (BS). We classified78 patients who underwentBS into four groups, as follows: Those evaluated 1–2 years after BS (BS2), 2–4 years after BS (BS4), 4–6 years after BS (BS6), and 6–10 years after BS (BS+6). Body weight (BW), body mass index (BMI), comorbidities associated with obesity (ACRO), physical activity level, and QoL were evaluated. Patients exhibited improvements in BW, BMI, cardiometabolic risk, hypertension, dyslipidemia, and diabetes and significant changes in lipid profiles in the first postoperative yearafter BS.The physical activity level inthe BS2, BS4, and BS6 groups was increased, compared with that in the first postoperative year, with a decrease in International Physical Activity Questionnaire scores at 1 year in the BS2 (207.50 ± 30.79), BS4 (210.67 ± 33.69), and BS6 (220.00 ± 42.78) groups. The QoL of patients in theBS2 and BS4 groups was excellent and that of patients in the BS4 and BS+6 groupswas very good. These findings suggest that BS promoted improved physical activity levels and QoL and reduced comorbidities in patients with morbid obesity.


2020 ◽  
Vol 12 (3) ◽  
pp. 103-113
Author(s):  
NEVZAT DEMIRCI ◽  
PERVIN TOPTAŞ DEMIRCI ◽  
OKTAY ZIRHLI

Background: Chronic diseases are the leading cause of death worldwide with increasing prevalence in the population aged 65 years and above. This study aimed to evaluate the relationship between physical activity level and health-related quality of life in elderly individuals. Material and methods: The participants aged 65 years and older (46 males and 35 females) were divided into 3 groups according to their physical activity levels: low physical activity (<150 minutes/week), moderate physical activity (150–300 minutes/week) and high physical activity level (>300 minutes/week). Health-related quality of life (HRQoL) was calculated using EQ-5D scale. Spearman's correlation coefficient was used to determine the relationship between physical activity level and HRQoL scores in elderly individuals. Results: A significant relationship and differences were found between high, moderate and low physical activity levels and HQQoL dimensions (P<0.05). The moderate and high physical activity groups were found to be significantly higher in all dimensions compared to the low physical activity group according to HRQoL scores (P<0.001). Conclusions: It has been concluded that high and moderate physical activity levels have a great positive relationship with HRQoL in individuals aged 65 years and older with a chronic disease.


2018 ◽  
Vol 3 (2) ◽  
pp. 62-77 ◽  
Author(s):  
Chaocheng He ◽  
Panhao Ma ◽  
Lusha Zhou ◽  
Jiang Wu

Abstract Purpose Compared with traditional course materials used in the classroom, the massive open online course (MOOC) forum that delivers unlimited learning content to students has various advantages. Yet MOOC has also received criticism recently, notably the problem of extremely low participation rates in its discussion forums. This study aims to explore the correlation between forum activity and student course grade in MOOC, and identify more accurately the forum activity levels of participants and the quality of threads in MOOC. Design/Methodology/Approach We crawled students’ tests, final exams, exercises, discussions performance data and total scores from a course in Chinese College MOOC from May 2014 to August 2014. And we use the data to analyze the correlation between Forum Participation and Course Performance based on nonparametric tests as well as multiple linear regressions with the software of R. The study provides definitions and algorithms of super degrees based on the supernetwork model to help find high-quality threads and active participants. Findings A positive correlation between forum activity and course grade is found in this study. Students who participate in the forum have better performance than those who do not. Using the definitions and algorithms of super degrees in the supernetwork, forum activity levels of participants as well as the quality of threads they employ are identified. Research limitation Only limited representative forum participants and threads are used to analyze the activity level and significance of the MOOC forum. Also, the study only investigates one Chinese course on information retrieval. More data and more data sources could be helpful in better understanding the MOOC forum phenomenon. Practical implications As super degrees can reveal more latent information and recognize high-quality threads as well as active participants, these parameters can be used to assess needs to improve forum settings and alleviate the problem of low forum participation. The proposed super degrees can be applied in social network domains for further research. Originality/Value Definitions and algorithms of super degrees are provided and used for forum analysis. Super degrees can be applied to find high-quality threads and active participants, which is beneficial to guide students to participate in these high-quality threads and have a better understanding of knowledge MOOC provides.


Author(s):  
Kerim Rüzgar ◽  
Nurettin Konar

<p>Aim of this research is to compare the relationship between the physical activity levels and quality of life of teachers working in secondary education institutions. A total of 331 secondary school teachers, including 205 men and 126 women from various branches, participated in our study in Batman province and secondary education institutions. The teachers who participated in the research were descriptive such as gender, age range, branch, chronic disease, and what if this disease was present. “International Physical Activity Questionnaire Short Form” for determining information and physical activity levels and “Nottingham Health Profile” questionnaires were applied for their quality of life. The statistical analysis of the data was done by using SPSS 20.0 program, and non- parametric tests such as Kruskal-Wallis, Mann Whitney U Test and Spearman Cholera test were used because the data were not normally distributed. 6.6% of secondary education teachers who participated in the study had a chronic illness, physical activity level according to gender, quality of life according to age groups, physical skill level of special talent teacher compared to other branch teacher and significant differences in quality of life (p &lt;0.05). There were no significant differences in other parameters and in the relationship between physical activity level and life satisfaction (p&gt; 0.05). As a result of the research, it has been concluded that the level of physical activity affects the level of physical activity of the gender and special education teachers and the other branch teachers, the quality of life is affected by the age group, and there is no significant relationship between the level of secondary school teachers' physical activity.</p><p> </p><p><strong> Article visualizations:</strong></p><p><img src="/-counters-/edu_01/0974/a.php" alt="Hit counter" /></p>


2017 ◽  
Vol 3 (4) ◽  
pp. 174
Author(s):  
Aslan Aydoğan ◽  
Cevdet Tınazcı

AbstractThis study was aimed to investigate the effect of regular physical activity level on quality of life. The sample of the study included 363 personnel working at Near East University during May-June 2015. Data of the study were collected with “Demographic Information Form”, “Short form of International Physical Activity Evaluation Survey” and “Quality of Life Scale SF-36”. Data were analyzed with SPSS package program. Percentage, frequency and chi-square values were calculated and manova, anova and post hoc tests were applied for data analysis in order to determine if there is a significant difference between the variables. According to the results, it was revealed that males are more active than females. It was also figured out that married academic personnels are more active than single academic personnels; single administrative personnels are more active than married administrative personnels. When the quality of lives of academic and administrative personnels are compared, the significant difference in social and mental dimensions was observed in the favor of administrative personnel.When physical activity levels of academic and administrative personnels considered based on their age group; it was found that the most active personnels are 30 and under the age of 30 in both two groups.When quality of life levels of university personnel are considered based on age group; it was revealed that there was a significant difference in social and mental dimensions for 31 and above age group when compared to 30 and below age group.As a result of this research, it was figured out that physical activity levels of males when compared to females and physical activity levels of married participants when compared to single participants are higher. In terms of quality of life, it was revealed that administrative personnel are more healty in social and mental dimensions when compared to academic personnel. When physical activity levels based on age group are considered, the most active participants were 30 and above the age of 30 in both groups. When quality of life of university personnels are compared based on age group, it was revealed that 31 and above age group has higher levels of quality of life in social and mental dimensions when comapred to 30 and below age group. Keywords: Physical activity, university personnel, quality of life.


2020 ◽  
Author(s):  
Christian Arinze Okonkwo ◽  
Peter Olarenwaju Ibikunle ◽  
Izuchukwu Nwafor ◽  
Andrew Orovwigho

BACKGROUND Quality of life (QoL), physical activity (PA) level and psychological profile (PF) of patients with serious mental illness have been neglected during patient’s management OBJECTIVE The purpose of this study was to determine the effect of selected psychotropic drugs on the QoL, PA level and PF of patients with serious mental illness METHODS A cross sectional survey involving one hundred and twenty-four subject [62 Serious Mental Illness (SMI) and 62 apparently healthy subjects as control] using purposive and consecutive sampling respectively .Questionnaires for each of the constructs were administered to the participants for data collation. Analysis of the data was done using non parametric inferential statistics of Mann-Whitney U independent test and Spearman’s rho correlation with alpha level set as 0.05. RESULTS Significant difference was recorded in the QoL (p<0.05) of patient with SMI and apparently healthy psychotropic naive participants. There was a significant correlation between the QoL (p<0.05) and PF of participants with SMI. Participants with SMI had significantly lower QoL than apparently healthy psychotropic naive subject. QoL of the healthy psychotropic naive group was better than those of the participants with SMI. Female participants with SMI had higher PA than their male counterparts CONCLUSIONS Psychological profiles of male participants with SMI were lower than male healthy psychotropic naive participants. Clinicians should take precaution to monitor the QoL, PA level and PF because the constructs are relevant in evaluation of treatment outcome.


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