scholarly journals Characterization of Glutamate-Mediated Hormonal Regulatory Pathway of the Drought Responses in Relation to Proline Metabolism in Brassica napus L.

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 512 ◽  
Author(s):  
Van Hien La ◽  
Bok-Rye Lee ◽  
Md. Tabibul Islam ◽  
Md. Al Mamun ◽  
Sang-Hyun Park ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Drought Stress ◽  
Brassica Napus ◽  
Stress Responses ◽  
Plant Stress ◽  
Dependent Manner ◽  
Proline Metabolism ◽  
Regulatory Pathway ◽  
Brassica Napus L ◽  
Reducing Potential

Proline metabolism influences the metabolic and/or signaling pathway in regulating plant stress responses. This study aimed to characterize the physiological significance of glutamate (Glu)-mediated proline metabolism in the drought stress responses, focusing on the hormonal regulatory pathway. The responses of cytosolic Ca2+ signaling, proline metabolism, and redox components to the exogenous application of Glu in well-watered or drought-stressed plants were interpreted in relation to endogenous hormone status and their signaling genes. Drought-enhanced level of abscisic acid (ABA) was concomitant with the accumulation of ROS and proline, as well as loss of reducing potential, which was assessed by measuring NAD(P)H/NAD(P)+ and GSH/GSSG ratios. Glu application to drought-stressed plants increased both salicylic acid (SA) and cytosolic Ca2+ levels, with the highest expression of calcium-dependent protein kinase (CPK5) and salicylic acid synthesis-related ICS1. The SA-enhanced CPK5 expression was closely associated with further enhancement of proline synthesis-related genes (P5CS1, P5CS2, and P5CR) expression and a reset of reducing potential with enhanced expression of redox regulating genes (TRXh5 and GRXC9) in a SA-mediated NPR1- and/or PR1-dependent manner. These results clearly indicate that Glu-activated interplay between SA- and CPK5-signaling as well as Glu-enhanced proline synthesis are crucial in the amelioration of drought stress in Brassica napus.

scholarly journals Comparative hormonal regulatory pathway of the drought responses in relation to glutamate-mediated proline metabolism in Brassica napus

2019 ◽  
Author(s):  
Van Hien La ◽  
Bok-Rye Lee ◽  
Md. Tabibul Islam ◽  
Sang-Hyun Park ◽  
Dong-Won Bae ◽  
...  
Keyword(s):  
Drought Stress ◽  
Stress Responses ◽  
Plant Stress ◽  
Proline Accumulation ◽  
List Type ◽  
Dependent Manner ◽  
Proline Metabolism ◽  
Regulatory Pathway ◽  
Plant Stress Responses ◽  
Enhanced Expression

AbstractProline metabolism influences metabolic and signaling pathway in regulating plant stress responses. This study aimed to characterize the physiological significance of glutamate (Glu)-mediated proline metabolism in the drought stress responses, focusing on the hormonal regulatory pathway. The responses of cytosolic Ca2+ signaling, proline metabolism and redox components to the exogenous application of Glu in well-watered or drought-stressed plants were interpreted in relation to endogenous hormone status and their signaling genes. Drought-enhanced abscisic acid (ABA) were concomitant with ROS and proline accumulation, accompanied by decreased NAD(P)H/NAD(P)+ and GSH/GSSG ratios. Exogenous Glu-feeding under drought resulted in an increase of salicylic acid (SA) with an antagonistic decrease of ABA. Glu-enhanced SA coincided with the highest expression of SA synthesis related gene ICS1 and Ca2+-dependent protein kinase CPK5. SA-enhanced CPK5 expression was closely associated with further enhancement of proline synthesis-related genes (P5CS1, P5CS2, and P5CR) expression. The Glu-activated proline synthesis was responsible for the reset of reducing potential with enhanced expression of redox regulating genes TRXh5 and GRXC9 in a SA-mediated NPR1- and/or PR1-dependent manner. These results clearly indicate that Glu-activated interplay between SA- and CPK5-signaling and Glu-enhanced proline synthesis are crucial in the amelioration of drought stress in B. napus.HighlightDrought-induced oxidative stress and symptom are developed by ABA-dependent mannerGlu-application increases endogenous SA level with an antagonistic decrease of ABADrought-induced proline accumulation was further enhanced by exogenous Glu-applicationGlu-enhanced proline synthesis accompanied with SA-mediated regulatory pathwayGlu-enhanced SA-modulated proline metabolism is an integrated process of redox control

scholarly journals Contrastive response of Brassica napus L. to exogenous salicylic acid, selenium and silicon supplementation under water stress

2015 ◽  
Vol 67 (2) ◽  
pp. 397-404 ◽  
Author(s):  
Ghader Habibi
Keyword(s):  
Lipid Peroxidation ◽  
Salicylic Acid ◽  
Drought Stress ◽  
Brassica Napus ◽  
Foliar Application ◽  
Dry Mass ◽  
Total Chlorophyll ◽  
Brassica Napus L ◽  
Positive Effect ◽  
Exogenous Salicylic Acid

The present research was designed to determine the effects of exogenous salicylic acid (SA), selenium (Se) and silicon (Si) on the resistance of canola (Brassica napus L. cv Okapi) seedlings to salt stress. Foliar application of SA (0.1 mM) in canola plants under drought stress for 25 days exhibited a significantly positive effect on shoot dry mass and raised the levels of total chlorophyll as well as boosting the activity of superoxide dismutase (SOD) and catalase (CAT). In addition, soil application of silicon (0.35 g Na2SiO3/kg soil) had ameliorative effects on canola root growth under drought. It is concluded that SA and Si enhanced the salt tolerance of canola by protecting the cell membrane against lipid peroxidation. However, the foliar application of Se (10 mg/l) had no ameliorative effects on canola growth and antioxidant capacity under drought stress, as could be judged by accumulation of malondialdehyde (MDA).

scholarly journals A Study of Proline Metabolism in Canola (Brassica napus L.) Seedlings under Salt Stress

Molecules ◽  
2012 ◽  
Vol 17 (5) ◽  
pp. 5803-5815 ◽  
Author(s):  
Mubshara Saadia ◽  
Amer Jamil ◽  
Nudrat Aisha Akram ◽  
Muhammad Ashraf
Keyword(s):  
Salt Stress ◽  
Brassica Napus ◽  
Proline Metabolism ◽  
Brassica Napus L

scholarly journals Impact of Foliar Application with Salicylic Acid on Biochemical Characters of Canola Plants under Cold Stress Condition

2016 ◽  
Vol 8 (1) ◽  
pp. 98-105 ◽  
Author(s):  
Hamed KESHAVARZ ◽  
Seyed Ali Mohammad MODARRES SANAVY ◽  
Ramin SADEGH GHOL MOGHADAM
Keyword(s):  
Salicylic Acid ◽  
Brassica Napus ◽  
Acid Treatment ◽  
Stress Condition ◽  
Foliar Application ◽  
Total Chlorophyll ◽  
Salicylic Acid Treatment ◽  
Biochemical Traits ◽  
Brassica Napus L ◽  
Salicylic Acid Concentration

In this study the effect of foliar application of salicylic acid on the chlorophyll content, antioxidant enzymes activity, and the content of solute protein and proline were investigated in two canola varieties (Brassica napus L., cv ‘RGS’ and ‘Licord’) leaves during 0, 24, and 48 hours after salicylic acid treatment. The results showed that the content of total chlorophyll was decreased in ‘RGS’ cultivar during the experiment and this process was related with increasing of salicylic acid concentration. The activity of superoxide dismutase, peroxidase, and also lipid peroxidation were increased significantly after 48 hours compared with the first day. The results of catalase activity showed that, this trait was decreased 24 hours after salicylic acid treatment and this decrease was related with salicylic concentration. The content of protein in both cultivars slightly changed and plants treated with salicylic acid had more protein content, by contrast proline was greatly affected by salicylic acid treatment and its content was the highest 24 hours after treatment. According to the present findings the application of salicylic acid has useful effects on the biochemical traits of Brassica napus cultivars. Therefore it may be effective for the improvement of plant growth in cold regions.

scholarly journals The Vascular Pathogen Verticillium longisporum Does Not Affect Water Relations and Plant Responses to Drought Stress of Its Host, Brassica napus

2017 ◽  
Vol 107 (4) ◽  
pp. 444-454 ◽  
Author(s):  
Daniel Teshome Lopisso ◽  
Jessica Knüfer ◽  
Birger Koopmann ◽  
Andreas von Tiedemann
Keyword(s):  
Drought Stress ◽  
Brassica Napus ◽  
Plant Growth ◽  
Water Relations ◽  
Growth Parameters ◽  
Molecular Genetic ◽  
Leaf Water Content ◽  
Plant Responses ◽  
Brassica Napus L ◽  
Verticillium Longisporum

Verticillium longisporum is a host-specific vascular pathogen of oilseed rape (Brassica napus L.) that causes economic crop losses by impairing plant growth and inducing premature senescence. This study investigates whether plant damage through Verticillium stem striping is due to impaired plant water relations, whether V. longisporum affects responses of a susceptible B. napus variety to drought stress, and whether drought stress, in turn, affects plant responses to V. longisporum. Two-factorial experiments on a susceptible cultivar of B. napus infected or noninfected with V. longisporum and exposed to three watering levels (30, 60, and 100% field capacity) revealed that drought stress and V. longisporum impaired plant growth by entirely different mechanisms. Although both stresses similarly affected plant growth parameters (plant height, hypocotyl diameter, and shoot and root dry matter), infection of B. napus with V. longisporum did not affect any drought-related physiological or molecular genetic plant parameters, including transpiration rate, stomatal conductance, photosynthesis rate, water use efficiency, relative leaf water content, leaf proline content, or the expression of drought-responsive genes. Thus, this study provides comprehensive physiological and molecular genetic evidence explaining the lack of wilt symptoms in B. napus infected with V. longisporum. Likewise, drought tolerance of B. napus was unaffected by V. longisporum, as was the level of disease by drought conditions, thus excluding a concerted action of both stresses in the field. Although it is evident that drought and vascular infection with V. longisporum impair plant growth by different mechanisms, it remains to be determined by which other factors V. longisporum causes crop loss.

scholarly journals Role of salicylic acid in heat stress tolerance in tri-genomic Brassica napus L.

2020 ◽  
Vol 33 (1) ◽  
pp. 13-20
Author(s):  
Muhammad Awais Ghani ◽  
Muhammad Mehran Abbas ◽  
Basharat Ali ◽  
Khurram Ziaf ◽  
Muhammad Azam ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Heat Stress ◽  
High Temperature ◽  
Brassica Napus ◽  
Biochemical Analysis ◽  
Foliar Application ◽  
Brassica Napus L ◽  
Elevated Activity ◽  
Heat Stress Tolerance

Tri-genomic Brassica napus L.wasdeveloped by the cross between Brassica napusand Brassica nigra. The crop is animportant source of vegetable seed oil in Pakistan,after cotton. The low oilseed rape yield is attributed to high temperature in the production zones. Interspecific hybridization using these two speciescan be helpful to produce heat resistant hybrids. On the other hand, it has been found that foliar application of different plant growth regulators can be used to reduce the heat stress in Brassica. The objectiveof this studywas to test the response of three different tri-genomic hybrids to high temperature stressat seedling stage. Seedlings were foliar sprayed with 0.13 mM salicylic acid (SA) prior to exposure tohigh temperatureat two true leaf stage. The plants were harvested after 30 days of sowing for growth and biochemical analysis. Plants ofV38 showed the highest values for all morphological traits and biochemical activities among the threehybrids. In general, plants exposed to the temperature stress exhibited a significant decline in growth, chlorophyll content and enzyme activity.Foliar application of SA significantly improved leaf and root biomass under heat stress.Further, antioxidativeenzyme activities significantly increased in response to SA either compared to control or to plants exposed to temperature stress.It is concluded thatapplication of salicylic acid elevated activity of antioxidative enzymes and was helpful in mitigating the detrimental effects of high temperature inoil seed rape.

scholarly journals Salicylic Acid Phytohormone Decreased the Toxicity Damage of Exogenous Lead Absorption in Seedlings of an Oilseed Plant, Brassica napus L. Grown in Hydroponic Conditions

2019 ◽  
pp. 1-15
Author(s):  
Mahdi Khozaei ◽  
Shiva Boroumand Jazi
Keyword(s):  
Salicylic Acid ◽  
Brassica Napus ◽  
Soluble Sugars ◽  
Lead Toxicity ◽  
Chlorophyll Synthesis ◽  
Dry Mass ◽  
Inhibitory Effects ◽  
Brassica Napus L ◽  
Carbohydrate Levels

Oilseed plant, Brassica napus L. seedlings grown in hydroponic condition with different concentrations of Pb were treated with salicylic acid (SA) to investigate the role of exogenous salicylic acid in alleviating lead toxicity on biochemical and physiological activities of the plant. The results showed that application of different concentrations of Pb increased soluble sugars and reduced carbohydrate levels significantly in roots and shoots of the plants. The stress induced by application of Pb triggered significant inhibitory effects on growth and chlorophyll synthesis induced on the production of protein and proline and enhanced the levels of antioxidant activity. Salicylic acid (SA) treated plants showed alleviation increasing total dry mass, leaf area, shoot and root length as well as leaf total chlorophyll content in responses to Pb stress. Results revealed the importance of salicylic acid (SA) activity in enabling plants to reduce the soluble sugars and increase of insoluble sugar in heavy -metal-stressed plants. The content of proline and proteins were also reduced in plants were treated with salicylic acid. Our data provide evidence that salicylic acid treatment decreased the activity of antioxidant enzymes in plants were exposed to different levels of Pb.

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