scholarly journals Insecticidal Activity of Hyoscyamus niger L. on Lucilia sericata Causing Myiasis

Plants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 655
Author(s):  
Esra Küpeli Akkol ◽  
Mert Ilhan ◽  
Esma Kozan ◽  
Fatma Tuğçe Gürağaç Dereli ◽  
Mustafa Sak ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Insecticidal Activity ◽  
Hplc Analysis ◽  
Folk Medicine ◽  
Lucilia Sericata ◽  
Alkaloid Content ◽  
Hyoscyamus Niger ◽  
Morphological Abnormalities ◽  
Alkaloid Extracts ◽  
Main Components

Background: Hyoscyamus niger L. (Solanaceae) generally known as henbane, is commonly distributed in Europe and Asia. In Turkey, henbane seeds have been used in folk medicine to remove worms from the eyes. The present study aimed to investigate the insecticidal activity of H. niger seeds. Methods: n-hexane, ethyl acetate, methanol and alkaloid extracts were prepared from the seeds of the plant and their insecticidal activities on Lucilia sericata larvae were evaluated. EC50 and EC90 values of the alkaloid extract were calculated and morphological abnormalities were investigated. Results: Alkaloid extract prepared from the seeds of this plant displayed significant insecticidal activity. EC50 values of H. niger seeds alkaloid extract were found to be 8.04, 8.49, 7.96 μg/mL against first, second and third instar, respectively. It was determined that malformations of larvae included damaged larvae with small size, contraction and weak cuticle. Furthermore, HPLC analysis was performed on alkaloid extract of H. niger seeds and main components of the extract were determined. It was determined that alkaloid extract mainly contain hyoscyamine and scopolamine. Conclusions: These results confirm the folkloric usage of the plant and suggest that the alkaloid content of the plant could be responsible for the insecticidal activity.

scholarly journals Phenolic Composition, Antioxidant and Anticancer Potentials of Extracts from Rosa banksiae Ait. Flowers

Molecules ◽  
2020 ◽  
Vol 25 (13) ◽  
pp. 3068 ◽  
Author(s):  
Chen Zeng ◽  
Siyuan Luo ◽  
Shiling Feng ◽  
Tao Chen ◽  
Lijun Zhou ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Pharmacological Activity ◽  
Hplc Analysis ◽  
Folk Medicine ◽  
Ornamental Plant ◽  
Natural Antioxidant ◽  
Phenolic Composition ◽  
Extraction Technology ◽  
Research Papers

Rosa banksiae Ait. (R. banksiae) is a traditional Chinese folk medicine and an ornamental plant. Most previous studies have focused on cultivation and utilization while there are few research papers on the pharmacological activity of R. banksiae. This study aimed to get a better understanding of R. banksiae by extracting polyphenols with fractionated extraction technology. The results showed that ethyl acetate phase (EAP) contained the most polyphenols, while water phase (WP) had the least. HPLC analysis indicated that rutin and luteolin-4′-O-glucoside existed in the EAP and butanol phase (BP), but quercetin was only detected in the EAP. Six phenolic compositions were not detected in WB. The antioxidant and anti-tumor abilities of the EAP and BP were excellent. The results revealed that R. banksiae possessed a great antioxidant capacity and was rich in polyphenols, thus indicating R. banksiae was suitable for being a natural antioxidant and an abundant source of polyphenols.

Isolation and HPLC Determination of the Chemical Components of Gentianella acuta (Michx.) Hulten

2018 ◽  
Vol 15 (1) ◽  
pp. 21-33
Author(s):  
Ying Wei ◽  
Yongqiao Liu ◽  
Yifan Hele ◽  
Weiwei Sun ◽  
Yang Wang ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
High Speed ◽  
Chemical Constituents ◽  
Folk Medicine ◽  
Gradient Elution ◽  
Chemical Components ◽  
Isolation And Characterization ◽  
Major Chemical ◽  
Main Components ◽  
First Time

Background: Gentianella acuta (Michx.) Hulten is an important type of medicinal plant found in several Chinese provinces. It has been widely used in folk medicine to treat various illnesses. However, there is not enough detailed information about the chemical constituents of this plant or methods for their content determination. Objective: The focus of this work is the isolation and characterization of the major chemical constituents of Gentianella acuta, and developing an analytical method for their determination. Methods: The components of Gentianella acuta were isolated using (1) ethanol extraction and adsorption on macroporous resin. (2) and ethyl acetate extraction and high speed countercurrent chromatography. A HPLC-DAD method was developed using a C18 column and water-acetonitrile as the mobile phase. Based on compound polarities, both isocratic and gradient elution methods were developed. Results: A total of 29 compounds were isolated from this plant, of which 17 compounds were isolated from this genus for the first time. The main components in this plant were found to be xanthones. The HPLC-DAD method was developed and validated for their determination, and found to show good sensitivity and reliability. Conclusion: The results of this work add to the limited body of work available on this important medicinal plant. The findings will be useful for further investigation and development of Gentianella acuta for its valuable medicinal properties.

scholarly journals CYTOTOXIC BIOACTIVE COMPOUNDS FROM CALOTROPIS GIGANTEA STEM BARK

2016 ◽  
Vol 8 (9) ◽  
pp. 111
Author(s):  
Kartini Hasballah ◽  
Murniana . ◽  
Erya . ◽  
Ardian .
Keyword(s):  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Ethyl Acetate Extract ◽  
Leukemia Cell ◽  
Stem Bark ◽  
Hexane Extract ◽  
Leukemia Cell Line ◽  
Calotropis Gigantea ◽  
Murine Leukemia Cell ◽  
Main Components

<p><strong>Objective: </strong>The present study deals with the cytotoxic activity of n-hexane and ethyl acetate extracts of <em>Calotropis gigantea</em> L. stem bark and its fractions such as A, B, C, D and E fractions on murine leukemia cell line P388.</p><p><strong>Methods: </strong>The crude extracts of <em>C. gigantea</em> stem bark were prepared using n-hexane and ethyl acetate solvents. The plant extracts were subjected to vacuum liquid chromatography followed by TLC. According to the similarity of stain patterns, the fractions were combined. The extracts and its combined fractions were then subjected for the phytochemical test. Cytotoxic activity of those extracts and its combined fractions were tested using MTT assay. Fraction D was subjected to gravity column chromatography followed by TLC. Then, fractions A, B, and D2 were crystallized and subjected to GC-MS.</p><p><strong>Results: </strong>The qualitative screening of n-hexane extract of <em>Calotropis gigantea</em> L. stem bark for secondary metabolites showed the presence of terpenoid, flavonoids, phenolics and coumarins. While the ethyl acetate extract contained phenolics, steroids, flavonoids, saponins and coumarins compounds. IC<sub>50 </sub>values for n-hexane extract and E fraction are 76.29 µg/ml and 18.48 µg/ml, respectively. In the ethyl acetate extract and C fraction obtained IC<sub>50</sub> values 57.05 µg/ml and 52.58 µg/ml.</p><p><strong>Conclusion: </strong>Cytotoxic activity from E fraction of n-hexane extract of <em>C. gigantea</em> stem bark is the most potent and containing flavonoids, phenolics and coumarins. The main components from several compounds of n-hexane extract of <em>C. gigantea</em> are germacrane-A, (-)-globulol, urs-12-ene and veridiflorol. </p>

HPLC Analysis and Antioxidant Properties of Algerian Lepidium draba Ethyl acetate Extract

2018 ◽  
Vol 8 (4) ◽  
pp. 265-271
Author(s):  
Bensaid Sara Ouissem ◽  
Bicha Sabrina ◽  
Benmekhebi Lotfi ◽  
Rebbas Khellaf ◽  
Bensouici Chawki ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Hplc Analysis ◽  
Ethyl Acetate Extract ◽  
Antioxidant Properties ◽  
Lepidium Draba

Tarczyca bocznokwiatowa (Scutellaria lateriflora) – znaczenie w medycynie tradycyjnej i pozycja we współczesnej fitoterapii

2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Inga Kwiecień ◽  
Beata Kawka ◽  
Halina Ekiert
Keyword(s):  
Chemical Composition ◽  
North American ◽  
Folk Medicine ◽  
Raw Material ◽  
Study Results ◽  
Phenylpropanoid Glycosides ◽  
Scutellaria Lateriflora ◽  
Sedative Action ◽  
Main Components

American skullcap (Scutellaria lateriflora L.) has long been used as a medicine by North American Indian tribes. A monograph of Scutellariae lateriflorae herba was included in the American Herbal Pharmacopoeia in 2009. This raw material is very popular in North America because of its sedative action. It is also highly valued in Australia, while in Europe, it has been widely used until now in the United Kingdom. The main components of the herb of this plant species include flavonoids specific for the Scutellaria genus, mostly baicalein, baicalin, wogonin, scutellarin. They are accompanied by other groups of compounds: diterpenoids – neoclerodan derivatives, γ-aminobutyric acid, melatonin, serotonin, catalpol, phenolic acids, phenylpropanoid glycosides (mostly verbascoside) and essential oil. Studies of chemical composition and investigation of medicinal activity of this raw material confirmed its uses known from North American folk medicine. Study results proved most of all a significance of flavonoid compounds present in the raw material for its documented biological activity. The aim of the paper is to review the present knowledge on chemical composition and medicinal significance of this very valuable plant. The research team from the Department of Pharmaceutical Botany JU MC initiated biotechnological studies of this species. Its microshoots successfully grown in vitro produce considerable amounts of specific flavonoids (mostly baicalin and wogonoside) and verbascoside, and in future they can be an alternative rich source of these compounds for pharmaceutical and cosmetic applications.

scholarly journals Comparison of Volatiles of Sideritis caesarea Specimens Collected from Different Localities in Turkey

2017 ◽  
Vol 12 (10) ◽  
pp. 1934578X1701201
Author(s):  
Tuğba Günbatan ◽  
Betül Demirci ◽  
İlhan Gürbüz ◽  
Fatih Demircib ◽  
Ayşe Mine Gençler Özkanc
Keyword(s):  
Gas Chromatography ◽  
Folk Medicine ◽  
Volatile Components ◽  
Chemical Compositions ◽  
Moderate Activity ◽  
Caryophyllene Oxide ◽  
Hexadecanoic Acid ◽  
Flame Ionization ◽  
Main Components

Sideritis caesarea H. Duman, Aytaç & Başer of the Lamiaceae is an Anatolian endemic species, and is mainly used as herbal tea and folk medicine. This present study aimed to determine and compare the chemical compositions of the volatile components of S. caesarea specimens collected from different localities in Kayseri province, Turkey: Sariz town (samples A and B), Pınarbaşı-Kaynar (sample C) and Pınarbaşı-Şirvan Mountain (sample D), respectively. Initially, the essential oils of the aerial parts were obtained by hydrodistillation and analysed both by gas chromatography-flame ionization detector (GC-FID), gas chromatography/mass spectrometer (GC/MS), simultaneously. Major volatile components of samples A and D were characterized as hexadecanoic acid (19.7 and 20.5%), caryophyllene oxide (6.7 and 20.2%), β-caryophyllene (6.5 and 12.6%), respectively. In sample B, hexadecanoic acid (14.6%), β-caryophyllene (11.5%) and caryophyllene oxide (8.3%) were detected as the major components. Whereas caryophyllene oxide (13.7%), hexadecanoic acid (8.5%) and spathulenol (6.1%) were the main components of the essential oil of sample C. Besides the chemical profiling, in vitro antimicrobial effects of samples were evaluated against a panel of six pathogenic microorganisms. Inhibition zones of the tested samples varied against bacteria with low to moderate activity, where no susceptibility against Candida albicans was observed.

Pharmacological and Pathological Studies on Taiwan Folk Medicine (IX): The Hepatoprotective Effect of the Methanolic Extract from Echinops Grijisii

1993 ◽  
Vol 21 (01) ◽  
pp. 33-44 ◽  
Author(s):  
Chun-Ching Lin ◽  
Cheng-Hung Lin
Keyword(s):  
Liver Biopsy ◽  
Ethyl Acetate ◽  
Liver Damage ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Folk Medicine ◽  
Hepatoprotective Activity ◽  
Albino Rats ◽  
Histopathological Changes ◽  
Wistar Albino Rats

In order to isolate the main hepatoprotective component of Echinops grijisii, the crude drug was extracted with methanol and subjected to continuous extractions using n-hexane chloroform, ethyl acetate and n-butanol. The hepatoprotective studies of each fraction from the methanol extract of E. grijisii was conducted in Wistar albino rats with CC14-induced liver damage. Hepatoprotective activity was evaluated in terms of the modification of serum transaminase values such as SGOT and SGPT, and histopathological changes of liver biopsy. The results indicated that the main hepatoprotective component was concentrated in n-butanol and aqueous fractions.

Evaluation of Lavandula stoechas L. subsp. stoechas L., Mentha spicata L. subsp. spicata L. essential oils and their main components against sinusitis pathogens

2018 ◽  
Vol 73 (9-10) ◽  
pp. 353-360 ◽  
Author(s):  
Nursenem Karaca ◽  
Betül Demirci ◽  
Fatih Demirci
Keyword(s):  
Gas Chromatography ◽  
Essential Oils ◽  
Folk Medicine ◽  
Gas Chromatography Mass Spectrometry ◽  
Mentha Spicata ◽  
Plant Materials ◽  
Lavandula Stoechas ◽  
Main Components

Abstract Lavandula stoechas subsp. stoechas and Mentha spicata subsp. spicata are used for the treatment of sinusitis in Turkish folk medicine. The components of essential oils obtained by hydrodistillation were determined by gas chromatography-flame ionization detector (GC-FID), gas chromatography/mass spectrometry (GC/MS), and thin layer chromatography (TLC). Major components of L. stoechas and M. spicata oils were determined as camphor (46.7%) and carvone (60.6%), respectively. The antibacterial activity of essential oils and their main components were tested against the common selected sinusitis pathogens Streptococcus pneumoniae, Streptococcus pyogenes, Staphylococcus aureus, Haemophilus influenzae, Moraxella catarrhalis, and Pseudomonas aeruginosa using in vitro agar diffusion, microdilution, and vapor diffusion methods. As a result, the tested plant materials, which are locally and natively used against sinusitis, were relatively mild antibacterial (in vitro MICs 310–1250 μg/mL) in action. To use essential oils and their components safely in sinusitis therapy, further detailed in vivo experiments are needed to support their efficacy.

scholarly journals Chemical composition and antioxidant, cytotoxic, and insecticidal potential of Valeriana alliariifolia in Turkey

2019 ◽  
Vol 70 (3) ◽  
pp. 207-218
Author(s):  
Burcu Sen-Utsukarci ◽  
Turgut Taskin ◽  
Fatih Goger ◽  
Nurhayat Tabanca ◽  
Alden S. Estep ◽  
...  
Keyword(s):  
Essential Oil ◽  
Insecticidal Activity ◽  
Folk Medicine ◽  
Added Value ◽  
Cytotoxic Activities ◽  
Dicaffeoylquinic Acid ◽  
The Difference ◽  
Ethanol Extracts ◽  
Dpph Scavenging ◽  
Bioactive Potential

AbstractValeriana is a common plant species used for various healing purposes in folk medicine since antiquity. This study investigates the phytochemical profile, antioxidant, cytotoxic, and insecticidal activity of Valeriana alliariifolia Adams, a species that has traditionally been used in Turkey. For the analyses we prepared four root extracts of V. alliariifolia Adams using hexane (HM1), chloroform (CM1), ethanol (EM1), and water (WM1) for maceration. Additionally, two extracts were also prepared from its roots by maceration separately with ethanol (EM2) and water (WM2). One sample was prepared as a water infusion (WI), according to the procedure used in Turkish traditional medicine. The 2,2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical cation scavenging activity tests showed that ethanol extracts had the strongest antioxidant activity: EM1 (IC50 – DPPH: 17.694 µg/mL; ABTS: 23.8 µg/mL) and EM2 (IC50 – DPPH: 20 µg/mL; ABTS: 21.5 µg/mL). The hexane extract, HM1, was the most cytotoxic (IC50<10 µg/mL against HepG2 and HUVEC) and EM2 strongly cytotoxic (IC50<10 µg/mL against HepG2 and IC50: 11.96 µg/mL against HUVEC). The extracts with demonstrated cytotoxic activities were further examined to check their insecticidal activity against adult female mosquito Aedes aegypti and first instar Ae. aegypti larvae. HM1 was the most effective (90±10 %), which was consistent with its cytotoxic activity. Because of the high antioxidant, cytotoxic, and insecticidal activities, we ran phytochemical analyses of the HM1, EM1, and EM2 extracts with GC-MS (for HM1) and LC-MS/MS (for EM1 and EM2). We also analysed the composition of the essential oil obtained from V. alliariifolia roots by micro-distillation in order to compare its content with HM1, which contains volatile compounds. Phytochemical analyses revealed that the major compound in HM1 was isovaleric acid (16 %) and in the essential oil 1,8-cineole (2.9 %). EM1 and EM2 contained 5-O-caffeoylquinic acid (chlorogenic acid), verbascoside (acteoside), and 3,5-dicaffeoylquinic acid as major components. In the light of our findings and available literature, we can conclude that V. alliariifolia has a good bioactive potential that could be used for different purposes, including the development of new agents for the treatment of various diseases. The difference in the content between the essential oil and HM1 was remarkable. It suggests that the variability observed in the activity of the samples was a result of composition and that, therefore, the aim of treatment should dictate which type of preparation is to be selected. An added value of our study is that it determined verbascoside and methylquercetin rutinoside for the first time in the Valeriana extracts.

scholarly journals Sedative and Anxiolytic Activities of Opuntia ficus indica (L.) Mill.: An Experimental Assessment in Mice

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1844
Author(s):  
Esra Küpeli Akkol ◽  
Mert Ilhan ◽  
Büşra Karpuz ◽  
Yasin Genç ◽  
Eduardo Sobarzo-Sánchez
Keyword(s):  
Ethyl Acetate ◽  
Methanolic Extract ◽  
Folk Medicine ◽  
Opuntia Ficus Indica ◽  
Spectral Techniques ◽  
Elevated Plus Maze Test ◽  
Board Test ◽  
Hole Board Test ◽  
Test Hole ◽  
Traction Test

Ethnobotanical field surveys revealed the use of fruits of Opuntia ficus indica (L.) Mill. for treating diabetes, burns, bronchial asthma, constipation, kidney stones, and rheumatic pains and as a sedative in Turkish folk medicine. This study aimed to verify the efficacy of the fruits of O. ficus indica experimentally and to define components responsible for the activity using bioassay-guided procedures. The crude methanolic extract of the fruits was sequentially fractionated into five subextracts: n-hexane, dichloromethane, ethyl acetate, n-butanol, and water. Further experiments were carried out on the most active subextract, that is, the ethyl acetate (EtOAc) subextract, which was further subjected to fractionation through successive column chromatographic applications on Sephadex LH-20. For activity assessment, each extract or fraction was submitted to bioassay systems; traction test, fireplace test, hole-board test, elevated plus-maze test, and open-field test were used for sedative and anxiolytic effects, and a thiopental-induced sleeping test was used for the hypnotic effect. Among the subextracts obtained from the methanolic extract, the EtOAc subextract showed significant sedative and anxiolytic effects in the bioassay systems. From the EtOAc subextract, major components were isolated, and their structures were determined as isorhamnetin, isorhamnetin 3-O-glucoside, isorhamnetin 3-O-rutinoside, and kaempferol 3-O-rutinoside using spectral techniques. In conclusion, this study confirmed the claimed use of the plant against anxiety in Turkish folk medicine.

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