scholarly journals Changes in the Content of Some Groups of Phenolic Compounds and Biological Activity of Extracts of Various Parts of Heather (Calluna vulgaris (L.) Hull) at Different Growth Stages

Plants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 926
Author(s):  
Victoria Chepel ◽  
Valery Lisun ◽  
Liubov Skrypnik
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Biological Activity ◽  
Phenolic Compounds ◽  
Calluna Vulgaris ◽  
Growth Stages ◽  
Flowering Stage ◽  
Leaf Extracts ◽  
Frap Assay ◽  
Different Growth Stages

Heather (Calluna vulgaris (L.) Hull.) is noted for a diverse chemical composition and a broad range of biological activity. The current study was aimed at monitoring changes in the accumulation of certain groups of phenolic compounds in various organs of heather (leaves, stems, roots, rhizomes, flowers, and seeds) at different growth stages (vegetative, floral budding, flowering, and seed ripening) as well as studying antioxidant (employing the DPPH and FRAP assays) and antibacterial activity of its extracts. The highest total amount of phenolic compounds, tannins, flavonoids, hydroxycinnamic acids, and proanthocyanidins was detected in leaves and roots at all growth stages, except for the flowering stage. At the flowering stage, the highest content of some groups of phenolic compounds (flavonoids, proanthocyanidins, and anthocyanins) was observed in flowers. Highest antioxidant activity was recorded for the flower extracts (about 500 mg of ascorbic acid equivalents per gram according to the DPPH assay) and for the leaf extract at the ripening stage (about 350 mg of ascorbic acid equivalents per gram according to the FRAP assay). Strong correlation was noted between antioxidant activity (DPPH) and the content of anthocyanins (r = 0.75, p ≤ 0.01) as well as between antioxidant activity (FRAP) and the total content of phenolic compounds (r = 0.77, p ≤ 0.01). Leaf extracts and stem extracts turned out to perform antibacterial action against both gram-negative and gram-positive bacteria, whereas root extracts appeared to be active only against B. subtilis, and rhizome extracts against E. coli.

scholarly journals Comparative Study on Antioxidant Activity of Salvia Nemorosa L. From Two Different Locations

2020 ◽  
Vol 28 (28) ◽  
pp. 17-21
Author(s):  
Elham Ghafiyehsanj ◽  
Kamaladdin Dilmaghani ◽  
Nader Chaparzade ◽  
Sara Saadatmand
Keyword(s):  
Antioxidant Activity ◽  
Comparative Study ◽  
Vegetative Stage ◽  
Growth Stages ◽  
Dpph Radical ◽  
Flowering Stage ◽  
Phenological Stages ◽  
Methanolic Extracts ◽  
Aerial Parts ◽  
Different Growth Stages

In order to investigate the antioxidant activity of Salvia nemorosa L. collected from Ahar and Urmia regions in Iran at different growth stages, aerial parts of sage after collecting were dried, and for measurement, the ability of scavenge DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) radical in different concentrations (0.025, 0.05, 0.07, 0.1, 0.2, 0.04 and 0.6) of methanolic extracts were prepared. The result showed that the ability to scavenge DPPH radical and amount of inhibition percent of vegetative stage leaves, flowering stage leaves, and flowers increased with increasing concentrations of methanolic extracts from 0.25 to 0.6 mg/ml. In the region of Urmia, the highest amount of DPPH inhibition there was in vegetative stage leaves, and the lowest amount of DPPH inhibition was seen in flowers. In Ahar regions, unlike the Urmia region, the highest amount of DPPH inhibition there was in flowers, but the lowest of DPPH inhibition was seen in flowering stage leaves. Also, the content of inhibition of DPPH in Ahar and Urmia regions similarly increased between two phenological stages (vegetative stage leaves, flowering stage leaves, and flowers) in 0.4 mg/ml and 0.6 mg/ml concentrations

scholarly journals COMPARATIVE STUDY ON ANTIOXIDANT ACTIVITY OF SALVIA NEMOROSA L. FROM TWO DIFFERENT LOCATIONS

2020 ◽  
Vol 28 (28) ◽  
pp. 17-21
Author(s):  
Elham GHAFIYEHSANJ ◽  
Kamaladdin DILMAGHANI ◽  
Nader CHAPARZADE ◽  
Sara SAADATMAND
Keyword(s):  
Antioxidant Activity ◽  
Comparative Study ◽  
Vegetative Stage ◽  
Growth Stages ◽  
Dpph Radical ◽  
Flowering Stage ◽  
Phenological Stages ◽  
Methanolic Extracts ◽  
Aerial Parts ◽  
Different Growth Stages

In order to investigate the antioxidant activity of Salvia nemorosa L. collected from Ahar and Urmia regions in Iran at different growth stages, aerial parts of sage after collecting were dried, and for measurement, the ability of scavenge DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) radical in different concentrations (0.025, 0.05, 0.07, 0.1, 0.2, 0.04 and 0.6) of methanolic extracts were prepared. The result showed that the ability to scavenge DPPH radical and amount of inhibition percent of vegetative stage leaves, flowering stage leaves, and flowers increased with increasing concentrations of methanolic extracts from 0.25 to 0.6 mg/mL. In the region of Urmia, the highest amount of DPPH inhibition there was in vegetative stage leaves, and the lowest amount of DPPH inhibition was seen in flowers. In Ahar regions, unlike the Urmia region, the highest amount of DPPH inhibition there was in flowers, but the lowest of DPPH inhibition was seen in flowering stage leaves. Also, the content of inhibition of DPPH in Ahar and Urmia regions similarly increased between two phenological stages (vegetative stage leaves, flowering stage leaves, and flowers) in 0.4 mg/mL and 0.6 mg/mL concentrations.

scholarly journals Capability of oxygen-scavenger sachets and modified atmosphere packaging to extend fresh barberry shelf life

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Razieh Niazmand ◽  
Samira Yeganehzad
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Phenolic Compounds ◽  
Shelf Life ◽  
Herbal Remedy ◽  
Physicochemical Characterization ◽  
Modified Atmosphere Packaging ◽  
Storage Period ◽  
Modified Atmosphere ◽  
Oxygen Scavenger

Abstract Background Barberry has long been used as an herbal remedy since ancient times which is found throughout temperate and subtropical regions of the world. Given the short harvesting season and limited shelf life of the barberry, we evaluated the possibility of using modified atmosphere packaging and oxygen-scavenger sachets to increase its storage period. For this purpose, the physicochemical characterization (antioxidant activity, anthocyanin, phenolic compounds, and ascorbic acid content, acidity, firmness, color, and decay incident) of fresh barberry samples packaged within different atmospheres was investigated over 4 weeks of storage at 4 and 25 °C. The barberries were packaged with low-density polyethylene/polyester (LDPE/PET) films under natural atmosphere (C), N2 gas (N), vacuum (V), or in the presence of an oxygen scavenger (OS). Results The results revealed that with increased storage period, the O2 and CO2 levels inside the packages decreased and increased, respectively. The antioxidant activity and amounts of anthocyanin, phenolic compounds, and ascorbic acid all decreased with increasing storage period. Among the studied atmospheres, the OS and, subsequently, V packages were most capable of maintaining the quality of fresh barberries, with the decay incidence being approximately 30 times lower inside these packages relative to the control. Increasing the storage temperature accelerated the intensity of chemical changes and decay across all samples. Conclusion The barberries inside the OS packages stored at 4 °C (and even at 25 °C) still had good sensory properties in terms of chemical properties, texture, and color after 4 weeks. Fortunately, it seems that this packaging technology makes the exportation and delayed consumption of the fresh barberry possible by maintaining its quality.

Aktywność biologiczna ekstraktów z propolisu

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Magdalena Woźniak ◽  
Anna Kwiatkowska ◽  
Elżbieta Hołderna-Kędzia ◽  
Katarzyna Sosnowska ◽  
Lucyna Mrówczyńska ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Biological Activity ◽  
Phenolic Compounds ◽  
Propylene Glycol ◽  
Ethyl Alcohol ◽  
Antiradical Activity ◽  
Colorimetric Method ◽  
Biological Properties ◽  
Bioactive Substances ◽  
Dpph Test

Introduction. Propolis, also known as bee glue, is a resinous material collected by honeybees with numerous biological properties, including antibacterial, antifungal, antioxidant and anticancer effects. Due to its health-promoting properties, propolis is a component of many products, including dietary supplements, cosmetics and healthy food. Aim. The aim of the study was to determine the antibacterial, antifungal and antioxidant activity of propolis extracts, as well as to compare the biological activity of propolis extracts, depending on the solvent used – ethyl alcohol or propylene glycol. Material and methods. Two propolis extracts were used in the research – the first was prepared in ethyl alcohol, and the second in propylene glycol. The antimicrobial activity of the examined extracts was determined against S. aureus, E. coli and C. albicans. The antioxidant activity was determined on the basis of the evaluation of their antiradical activity in the DPPH· test and Fe2+ chelating activity. Moreover, the total content of phenolic compounds and flavonoids in the tested extracts was determined using the colorimetric method. Results. The tested propolis extracts, regardless of the solvent used (ethyl alcohol or propylene glycol), showed high antibacterial (against S. aureus), antifungal (against C. albicans) and antioxidant (antiradical activity in the DPPH· test and ferrous iron chelating potency in the ferrozine test) activity. Moreover, both tested extracts were characterized by a high and similar content of bioactive compounds – phenolic compounds and flavonoids. Conclusions. The results of the conducted tests showed that the solvent used did not affect determined biological activity and the content of bioactive substances in the tested propolis extracts.

scholarly journals Antioxidant Compounds, Mineral Content and Antioxidant Activity of Several Tomato Cultivars Grown in Southwestern Romania

2013 ◽  
Vol 41 (1) ◽  
pp. 136 ◽  
Author(s):  
Violeta NOUR ◽  
Ion TRANDAFIR ◽  
Mira E. IONICA
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Trace Elements ◽  
Phenolic Compounds ◽  
Total Phenolics ◽  
Antioxidant Activities ◽  
Fruit Shape ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Tomato Cultivars

Ten indeterminate tomato cultivars differing in fruit shape and typology, grown simultaneously in a greenhouse from Oltenia (Southwestern Romania) and harvested at red ripe stage, were investigated for the contents in ascorbic acid, lycopene, β-carotene, total phenolics, minerals (K, Na, Ca and Mg) and trace elements (Fe, Cu, Mn, Cr, Zn and B). In addition, their antioxidant activities were determined. Significant differences were found among tomato cultivars in all studied antioxidant compounds, as well as in the antioxidant activity. Ascorbic acid ranged from 91.9 to 329.7 mg kg-1 fw, lycopene ranged from 19.7 to 49.0 mg kg-1 fw, while total phenolic compounds varied between 300.2 and 557.8 mg kg-1 fw. Antioxidant activity ranged from 0.81 mmol Trolox kg-1 fw to 1.74 mmol Trolox kg-1 fw and it was significantly correlated to total phenolics content (r = 0.91; p< 0.05). The cocktail type cultivar ‘Tiger’ and the cherry type cultivar ‘Belle’ proved to be the most powerful in antioxidant activity and phenolic compounds while the rectangular plum shaped cultivar ‘Porto’ recorded the highest average lycopene content. A valuable cultivar proved to be the brownish red ‘Sacher’ which registered among the highest contents of phenolics, lycopene and ascorbic acid. Mineral and trace elements contents were also significantly affected by cultivars. Values recorded for K, Ca and Mg ranged from 2139.6 to 3056.9 mg kg-1, 137.7 to 325.8 mg kg-1 and 27.3 to 168.7 mg kg-1 respectively.

scholarly journals Water and heat unit requirement in different growth stages of Soybean (Glycine max L. Merrill) at Bhopal

MAUSAM ◽  
2021 ◽  
Vol 58 (4) ◽  
pp. 537-542
Author(s):  
I. J. VERMA ◽  
H. P. DAS ◽  
V. N. JADHAV
Keyword(s):  
Crop Coefficient ◽  
Growth Stages ◽  
Maturity Stage ◽  
Degree Days ◽  
Flowering Stage ◽  
Heat Unit ◽  
Soybean Crop ◽  
Pod Development ◽  
Use Efficiency ◽  
Different Growth Stages

In this study, ET data available on Soybean crop for Bhopal during 1991-95 have been utilized.  With regard to water need of the crop, the life span of soybean has been divided into five important growth stages viz., seedling up to 2 weeks after sowing (WAS), vegetative (3-8 WAS), flowering (9-10 WAS), pod development (11-13 WAS), and maturity (14-15 WAS). In this paper, consumptive use of water (ET), Water Use Efficiency (WUE), Heat Units (HU), Heat Use Efficiency (HUE) and crop coefficient (Kc) for different growth stages of the crop have been computed and discussed.                The study revealed that on an average, Soybean crop consumed about 450 mm of water. The average WUE was found to be 3.23 kg /ha/mm. It was also observed that WUE does not depend only on the total amount of water consumed by the crop but also indicates the importance of its distribution during various growth stages. On an average, the crop consumed nearly 7%, 36%, 24%, 25% and 8% of water during seedling, vegetative, flowering, pod development and maturity stage respectively. The crop consumed maximum amount of water during vegetative stage. However, the average weekly ET rate was found to be highest during flowering stage (nearly 52 mm). Average heat unit requirement of soybean was found to be 1694 degree-days. Maximum heat units were required during vegetative stage (638 degree days) followed by pod development stage (358 degree days). The average HUE was found to be 0.86 kg/ha/degree days. Crop coefficient (Kc) values varied in the range 0.30 – 0.45, 0.55 – 0.90, 1.00 – 1.15, 0.85 – 0.70 and 0.55 – 0.40 during seedling, vegetative, flowering, pod development and maturity stage respectively. The crop coefficient values attained the peak during the flowering stage.  

scholarly journals THE ANTIOXIDANT STUDIES OF TWO MEDICINAL PLANTS, SPHAERANTHUS INDICUS AND PSOPHOCARPUS TETRAGONOLOBUS

2019 ◽  
Vol 12 (1) ◽  
pp. 321
Author(s):  
Vijayalakshmi N ◽  
Mudiganti Ram Krishna Rao
Keyword(s):  
Ascorbic Acid ◽  
Medicinal Plants ◽  
Antioxidant Activities ◽  
Psophocarpus Tetragonolobus ◽  
Leaf Extracts ◽  
Frap Assay ◽  
Methanol Fraction ◽  
Reducing Ability ◽  
Ic50 Value ◽  
Sphaeranthus Indicus

Objective: The present study deals with the antioxidant assays of the different leaf extracts of two medicinal plants, Sphaeranthus indicus and Psophocarpus tetragonolobus. Methods: Dried leaves of S. indicus and P. tetragonolobus were packed in separate round bottom flasks for sample extraction using ethanol, methanol, hexane, and distilled water as solvents for 72 h, and the extracts were collected after evaporating the solvents. Antioxidant studies of the various extracts were performed by 1-diphenyl-2-picrylhydrazyl and Ferric Reducing Ability of Plasma assays.Results: Among the two plants studied, S. indicus showed better 2-diphenyl-1-picryl-hydrazyl (DPPH), scavenging activity than P. tetragonolobus with IC50 values of 174.380 and 262.313, respectively, as compared to that of the standard, ascorbic acid, IC50 value of which being 111.16. The FRAP assay results for both the plants indicated that the methanol fractions showed closer results when compared with standards, ascorbic acid and quercetin. The IC50 value of S. indicus, P tetragonolobus, ascorbic acid, and quercetin was 70.065, 151.953, 85.162, and 79.647, respectively. These results clearly indicate that S. indicus methanol fraction had better antioxidant activity when compared to both standards.Conclusion: It is concluded that S. indicus and P. tetragonolobus have excellent antioxidant activities which could be the major contributing factors for their medicinal roles. Further studies in this direction are being carried on.

scholarly journals Berries and Leaves of Actinidia kolomikta (Rupr. & Maxim.) Maxim.: A Source of Phenolic Compounds

Plants ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 147
Author(s):  
Laima Česonienė ◽  
Paulina Štreimikytė ◽  
Mindaugas Liaudanskas ◽  
Vaidotas Žvikas ◽  
Pranas Viškelis ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Phenolic Compounds ◽  
Radical Scavenging ◽  
Total Content ◽  
Ascorbic Acid Content ◽  
Free Radical Scavenging ◽  
Raw Material ◽  
Quantitative Composition ◽  
Pharmaceutical Industries

Berries of Actinidia kolomikta (A. kolomikta) are known for high ascorbic acid content, but the diversity of phenolic compounds has been little studied. The present research aimed to investigate phenolic compounds and antioxidant activity in berries and leaves of twelve A. kolomikta cultivars. The UHPLC-ESI-MS/MS technique was used to determine differences among cultivars in the quantitative composition of individual phenolic compounds. Antioxidant activity was determined by DPPH• free radical scavenging and CUPRAC methods. In the present study, 13 phenolic compounds were detected in berries, whereas leaves contained 17 phenolic compounds. Flavonols were the primary class found in both berries and leaves; other identified phenolic compounds were flavan-3-ols, flavones and, phenolic acids; and dihydrochalcone phloridzin was identified in the leaves. The amount and variety of phenolic compounds in berries and leaves and antioxidant activity were found to be cultivar-dependent. The highest total content of phenolic compounds was found in the leaves of the cultivar ‘Aromatnaja’ and in the berries of the cultivar ‘VIR-2’. Results of this study have confirmed that berries and leaves of A. kolomikta could be a valuable raw material for both food and pharmaceutical industries.

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