In Vitro Assessment of the Cell Metabolic Activity, Cytotoxicity, Cell Attachment, and Inflammatory Reaction of Human Oral Fibroblasts on Polyetheretherketone (PEEK) Implant–Abutment

The purpose of this research is to compare the cytotoxicity of polyetheretherketone (PEEK) and polyetherketoneketone (PEKK) with conventional dental implant–abutment materials, namely titanium alloy (Ti-6Al-4V) and yttria-stabilized tetragonal zirconia polycrystal (Y-TZP), to evaluate the cell metabolic activity, cytotoxicity, and inflammation potential of human oral fibroblasts (HOF) on these materials. Disk-shaped specimens were designed and prepared via a dental computer-aided manufacturing technology system. Surface topography, roughness, and free energy were investigated by atomic force microscope and contact angle analyzer; cell metabolic activity and cytotoxicity by MTT assay; and morphological changes by scanning electron microscopy (SEM). The effect of pro-inflammatory gene expression was evaluated by RT-qPCR. The obtained data were analyzed with one-way analysis of variance and post-hoc Tukey’s honest significant difference tests. PEEK and PEKK exhibited higher submicron surface roughness (0.04 μm) and hydrophobicity (>80°) than the control. Although the cell activity of PEEK was lower than that of Ti-6Al-4V and Y-TZP for the first 24 h (p < 0.05), after 48 h there was no difference (p > 0.05). According to the cell cytotoxicity and the pro-inflammatory cytokine gene expression assays, there was no difference between the materials (p > 0.05). SEM observations indicated that HOF adhered poorly to PEKK but properly to Ti-6Al-4V, Y-TZP, and PEEK. PEEK and PEKK show comparable epithelial biological responses to Ti-6Al-4V and Y-TZP as implant–abutment materials. Between the two polymeric materials, the PEEK surface, where the HOF showed better cell metabolic activity and cytotoxicity, was a more promising implant–abutment material.

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Micropatterned Silica Films with Nanohydroxyapatite for Y-TZP Implants

Journal of Dental Research ◽

10.1177/0022034518765762 ◽

2018 ◽

Vol 97 (9) ◽

pp. 1003-1009 ◽

Cited By ~ 3

Author(s):

R.B.P. Miranda ◽

L. Grenho ◽

A. Carvalho ◽

M.H. Fernandes ◽

F.J. Monteiro ◽

...

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Metabolic Activity ◽

Tetragonal Zirconia ◽

Likelihood Method ◽

Biological Behavior ◽

Cell Metabolic Activity ◽

Mg63 Cells ◽

Significant Difference ◽

Scanning Electron

This investigation aimed at developing micropatterned silica thin films (MSTFs) containing nanohydroxyapatite (nano-HA) microaggregates that were not completely covered by silica so that they could directly interact with the surrounding cells. The objectives were 1) to evaluate the effect of the presence of 2 films (MSTF with or without nano-HA addition) on the characteristic strength (σ0) and Weibull modulus ( m) of a yttria-stabilized tetragonal zirconia polycrystal (Y-TZP) and 2) to evaluate the effect of these 2 films, as applied onto the Y-TZP surface, on the morphology, orientation, and proliferation of MG63 cells. Sol-gel process and soft lithography were used to apply the MSTF onto the Y-TZP specimens. Three experimental groups were produced: Y-TZP, Y-TZP + MSTF, and Y-TZP + MSTF + sprayed nano-HA. All surfaces were characterized by scanning electron microscopy and energy-dispersive X-ray spectroscopy and tested for 4-point flexural strength ( n = 30) in water at 37 °C. Weibull analysis was used to determine m and σ0 (maximum likelihood method). In vitro biological behavior was performed with human osteoblast-like cells (MG63). Y-TZP was successfully coated with MSFT and MSFT + nano-HA. Scanning electron microscopy micrographs indicated that the microaggregates of nano-HA were not entirely covered by the silica. There was no statistically significant difference among the experimental groups for σ0 and m. In the groups containing the films, the cells were elongated and aligned along the lines. The MSFT + nano-HA group showed significantly higher cell metabolic activity than that obtained for the Y-TZP group at day 7. This investigation was successful in producing an MSTF containing nano-HA microaggregates that remained exposed to the environment. The developed films did not jeopardize the structural reliability of a commercial Y-TZP, as confirmed by the Weibull statistics. The MG63 cells seeded over the films became elongated and aligned along the films’ micropatterned lines. Y-TZP specimens coated with MSTF and nano-HA showed a higher cell metabolic activity and proliferation after 7 d of culture when compared with uncoated Y-TZP.

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Regenerative Potential of Blood-Derived Products in 3D Osteoarthritic Chondrocyte Culture System

Current Issues in Molecular Biology ◽

10.3390/cimb43020048 ◽

2021 ◽

Vol 43 (2) ◽

pp. 665-675

Author(s):

Olga Kuten-Pella ◽

Andrea De Luna ◽

Karina Kramer ◽

Markus Neubauer ◽

Stefan Nehrer ◽

...

Keyword(s):

Gene Expression ◽

Molecular Mechanisms ◽

Platelet Rich Plasma ◽

Cell Activity ◽

Blood Products ◽

Tissue Quality ◽

Xtt Assay ◽

Matrix Deposition ◽

Genes Expression ◽

Significant Difference

Intra-articular injection of different types of blood-derived products is gaining popularity and clinical importance in the treatment of degenerative cartilage disorders such as osteoarthritis. The regenerative potential of two types of platelet-rich plasma (PRP), prepared in the presence of EDTA (EPRP) and citrate (CPRP) and an alternative blood product-hyperacute serum (hypACT) was evaluated using a 3D osteoarthritic chondrocyte pellet model by assessing the metabolic cell activity, cartilage-related gene expression and extracellular matrix deposition within the pellets. Chondrocyte viability was determined by XTT assay and it revealed no significant difference in metabolic activity of OA chondrocyte pellets after supplementation with different blood products. Nevertheless, the selection of blood products influenced the cartilage-related genes expression, ECM morphology and the tissue quality of pellets. Both PRP types had a different biological effect depending upon concentration and even though CPRP is widely used in clinics our assessment did not reveal good results in gene expression either tissue quality. HypACT supplementation resulted in superior cartilage-related genes expression together with tissue quality and seemed to be the most stable product since no remarkable changes were observed between the two different concentrations. All in all, for successful regenerative therapy, possible molecular mechanisms induced by blood-derived products should be always carefully investigated and adapted to the specific medical indications.

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Morphologic and anatomic response, catalase gene expression in drought varieties of tomato and bioinformatics analyses of microarray studies of the catalase gene

10.21203/rs.3.rs-888851/v1 ◽

2021 ◽

Author(s):

Hanieh Mohajjel Shoja ◽

Taha Khezriani ◽

Maryam Kolahi ◽

Elham Elham Mohajel Kazemi ◽

Milad Yazdi

Keyword(s):

Gene Expression ◽

Drought Stress ◽

Real Time ◽

Morphological Changes ◽

Field Capacity ◽

Bioinformatics Analyses ◽

Catalase Gene ◽

Significant Difference ◽

Drought Conditions ◽

Microarray Studies

Abstract Crops in arid and semi-arid regions are exposed to adverse environmental factors such as drought. Experiments were conducted to determine the morphologic and anatomic response of drought-susceptible and tolerant varieties of tomato (Solanum lycopersicum L.) under drought conditions (100%, 75%, 50%, 25% of field capacity). To investigate the role of antioxidant enzyme, catalase gene expression was examined by real-time RT-qPCR and microarray studies of the catalase gene in tomatoes under stress examined utilizing bioinformatics. The results showed significant morphological changes under drought conditions. Anatomical studies revealed that CaljN3 is more resistant than SuperstrainB varieties under drought stress. Relative expression of the CAT1 gene did not show any significant difference in both Caljn3 and SuperstrainB varieties based on quantitative Real-Time PCR, under drought stress. The bioinformatics results from microarray analysis revealed that this gene did not show a significant difference in expression in any of the cultivars and under any of the stresses. This gene is in the conserve cluster, a cluster with 118 members and a z score of 14.26148. This showed that this cluster is fully protected between two susceptible and tolerant varieties. The enrichment gene of this cluster did not show any significant intracellular pathways. It appears that in response to stress, an activating mechanism other than catalase is necessary. The fight against oxidative stress may begin one step before that of the enzymes and seeks to combat the stressor by activating proteins, especially channels, pumps and some cellular messengers.

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Cytokine gene expression following topical exposure of mice to chemical contact and respiratory allergens

Immunology Letters ◽

10.1016/s0165-2478(97)87466-4 ◽

1997 ◽

Vol 56 (1-3) ◽

pp. 157

Author(s):

E Warbrick

Keyword(s):

Gene Expression ◽

Cytokine Gene Expression ◽

Cytokine Gene

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High single-cell metabolic activity in Antarctic sea ice bacteria

Aquatic Microbial Ecology ◽

10.3354/amea1205 ◽

2008 ◽

Author(s):

A Martin ◽

JA Hall ◽

R O’Toole ◽

SK Davy ◽

KG Ryan

Keyword(s):

Sea Ice ◽

Single Cell ◽

Metabolic Activity ◽

Cell Metabolic Activity ◽

Antarctic Sea Ice

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Faculty Opinions recommendation of Multiple signaling pathways contribute to synergistic TLR ligand-dependent cytokine gene expression in human monocyte-derived macrophages and dendritic cells.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1156938.617001 ◽

2009 ◽

Author(s):

Stephen Schwartz

Keyword(s):

Gene Expression ◽

Dendritic Cells ◽

Signaling Pathways ◽

Human Monocyte ◽

Cytokine Gene Expression ◽

Cytokine Gene ◽

Multiple Signaling

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Faculty Opinions recommendation of Impact of degradable macromer content in a poly(ethylene glycol) hydrogel on neural cell metabolic activity, redox state, proliferation, and differentiation.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.5382959.5337059 ◽

2010 ◽

Author(s):

Jennifer Elisseeff

Keyword(s):

Ethylene Glycol ◽

Metabolic Activity ◽

Redox State ◽

Neural Cell ◽

Poly Ethylene Glycol ◽

Cell Metabolic Activity ◽

Proliferation And Differentiation ◽

Poly Ethylene

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ATF4, DLX3, FRA1, MSX2, C/EBP-ζ, and C/EBP-α Shape the Molecular Basis of Therapeutic Effects of Zoledronic Acid in Bone Disorders

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520620666200721101904 ◽

2020 ◽

Vol 20 (18) ◽

pp. 2274-2284

Author(s):

Faroogh Marofi ◽

Jalal Choupani ◽

Saeed Solali ◽

Ghasem Vahedi ◽

Ali Hassanzadeh ◽

...

Keyword(s):

Gene Expression ◽

Zoledronic Acid ◽

Mrna Expression ◽

Promoter Methylation ◽

Methylation Level ◽

Target Genes ◽

Osteoblastic Differentiation ◽

Therapeutic Effects ◽

Significant Difference ◽

Scheduled Time

Objective: Zoledronic Acid (ZA) is one of the common treatment choices used in various boneassociated conditions. Also, many studies have investigated the effect of ZA on Osteoblastic-Differentiation (OSD) of Mesenchymal Stem Cells (MSCs), but its clear molecular mechanism(s) has remained to be understood. It seems that the methylation of the promoter region of key genes might be an important factor involved in the regulation of genes responsible for OSD. The present study aimed to evaluate the changes in the mRNA expression and promoter methylation of central Transcription Factors (TFs) during OSD of MSCs under treatment with ZA. Materials and Methods: MSCs were induced to be differentiated into the osteoblastic cell lineage using routine protocols. MSCs received ZA during OSD and then the methylation and mRNA expression levels of target genes were measured by Methylation Specific-quantitative Polymerase Chain Reaction (MS-qPCR) and real.time PCR, respectively. The osteoblastic differentiation was confirmed by Alizarin Red Staining and the related markers to this stage. Results: Gene expression and promoter methylation level for DLX3, FRA1, ATF4, MSX2, C/EBPζ, and C/EBPa were up or down-regulated in both ZA-treated and untreated cells during the osteodifferentiation process on days 0 to 21. ATF4, DLX3, and FRA1 genes were significantly up-regulated during the OSD processes, while the result for MSX2, C/EBPζ, and C/EBPa was reverse. On the other hand, ATF4 and DLX3 methylation levels gradually reduced in both ZA-treated and untreated cells during the osteodifferentiation process on days 0 to 21, while the pattern was increasing for MSX2 and C/EBPa. The methylation pattern of C/EBPζ was upward in untreated groups while it had a downward pattern in ZA-treated groups at the same scheduled time. The result for FRA1 was not significant in both groups at the same scheduled time (days 0-21). Conclusion: The results indicated that promoter-hypomethylation of ATF4, DLX3, and FRA1 genes might be one of the mechanism(s) controlling their gene expression. Moreover, we found that promoter-hypermethylation led to the down-regulation of MSX2, C/EBP-ζ and C/EBP-α. The results implicate that ATF4, DLX3 and FRA1 may act as inducers of OSD while MSX2, C/EBP-ζ and C/EBP-α could act as the inhibitor ones. We also determined that promoter-methylation is an important process in the regulation of OSD. However, yet there was no significant difference in the promoter-methylation level of selected TFs in ZA-treated and control cells, a methylation- independent pathway might be involved in the regulation of target genes during OSD of MSCs.

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