Comparison of Polymeric Cyanoacrylate Adhesives with Suturing in Free Gingival Graft Stability: A Split Mouth Trial

The aim is to compare the use of Cyanoacrylate adhesives (CAA) to the conventional suturing technique in terms of free gingival grafts (FGG) stability and healing in lower anterior and premolar regions. A split mouth design was initiated on 22 participants. Each side (from 2nd premolar to central incisor) was randomized to either the control or test groups. In the control group, sutures were used to stabilize the FGG, while, in the test group, the FGG was stabilized with butyl-cyanoacrylate. Full-periodontal clinical parameters were employed to assess the periodontal health. FGG-related parameters assessed included the keratinized tissue width (KTW), gingival tissue thickness (GTT), FGG shrinkage% and pain using the VAS score. No significant differences in the mean values of the KTW nor FGG shrinkage% across six time points (p < 0.05) were observed, whereas highly significant differences in the mean values of GTT across six time points (F = 3.32; p = 0.008) were observed. The use of CAA in FGG stability and healing is comparable to conventional suturing for soft tissue grafts in terms of success outcomes. With its cost effectiveness, lesser time consumption, post-operative pain and comparable graft stability and dimensions, the use of CAA may be a promising alternative for conventional and microsurgical techniques for the stabilization of FGG in the oral cavity.

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171IMPROVEMENT IN EMBRYO RECOVERY USING UTERINE DOUBLE FLUSHING

Reproduction Fertility and Development ◽

10.1071/rdv16n1ab171 ◽

2004 ◽

Vol 16 (2) ◽

pp. 207 ◽

Cited By ~ 1

Author(s):

A. Castro Neto ◽

B.V. Sanches ◽

S.H.V. Perri ◽

M.M. Seneda ◽

J.F. Garcia

Keyword(s):

Recovery Rate ◽

Foley Catheter ◽

Test Group ◽

Control Group ◽

Recovery Rates ◽

Mean Values ◽

Embryo Recovery ◽

Group A ◽

Uterine Flushing ◽

The Mean

The aim of the present study was to evaluate the effects of uterine double flushing on embryo recovery rates (total structures and viable embryos), after a resting period with the uterus filled with PBS. There were 210 embryo recovery procedures conducted using the uterine double flushing method, and the results were compaired with 432 conventional single flushing procedures. All procedures were conducted with Limousin (n=403) and Guzerá (n=239) cows, following the same superovulation protocol. Cyclic donors received a progestagen implant (CIDR) and 1mL of oestradiol benzoate (Estrogin®) at Day 0. Between Day 5 and Day 9 animals received doses ranging from 200 to 300UI (zebu cows) and 300 to 500UI (taurine cows) of FSH (Pluset® – Serono) in decreasing doses. Between Day 6 and Day 8 PGF2a (Ciosin®) was administered, followed by withdrawal of the progestagen after 24h. Artificial insemination was performed between 14 and 26h after the beginning of treatment. For the double flushing procedure, after the first uterine flushing of both horns with 1L DPBS (Nutricell), a Foley catheter was positioned in the uterine body in order to fill the uterus with the same solution (80 to 150mL). After this procedure the catheter was closed with a disposable 5-mL syringe gasket, and the animals were allowed to rest in the surroundings of the work place for 30min. After this period, a second flush was performed in order to recover the remaning liquid used during manipulation of the uterus. Animals from the control group (group A) were subjected to a single uterine flushing procedure. From 210 double flushing procedures (group B – test), 1409 viable embryos were produced, classified as grades I, II, III and IV (IETS), (average of 6.7 embryos per procedure), whereas, in the 432 single flushing procedure (group A – control), 1993 embryos were produced (average of 4.6). Statistical analysis showed the increase of viable embryo recovery rate. When consecutive double flushing was performed, the average of recovered embryos increased from 8.3 to 12.7 (P<0.05) in Limousin cows, and from 7.9 to 11.5 (P<0.05) in Guzerá cows. Comparing recovery after single flushing with that after double flushing, the mean number of viable embryos increased from 4.7 to 6.9 (P<0.05) in Limousin cows and from 4.5 to 6.4 (P<0.05) in Guzerá cows. In order to assure the nonexistence of negligence effects or operator influence on results, the mean values of total embryo recovery rate after single flushing (control group) was compared to the mean values of the same rate after the first uterine flushing on test group. Results indicated no difference between recovery rates. The present work showed the viability of using the uterine double flushing procedure for improvement of embryo recovery rates in cattle.

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Influence of classic massage on cardiac autonomic modulation

Fisioterapia em Movimento ◽

10.1590/1980-5918.029.003.ao06 ◽

2016 ◽

Vol 29 (3) ◽

pp. 487-496 ◽

Cited By ~ 1

Author(s):

Mário Augusto Paschoal

Keyword(s):

Test Group ◽

Well Being ◽

Control Group ◽

Autonomic Modulation ◽

Cross Sectional ◽

Mean Values ◽

Cardiac Autonomic Modulation ◽

Body Tissues ◽

Study Results ◽

The Mean

Abstract Introduction: Massage can be defined as the rhythmic and smooth manipulation of body tissues, with the aim to promote health and well-being. Objective: To assess the influence of classic massage on cardiac autonomic modulation. Methods: Cross-sectional study that evaluated healthy participants, with mean age between 18 and 25 years, divided into two groups: test group (TG, n=11) and control group (CG, n=10). The TG had their heartbeat recorded for 5min before receiving a classic massage for 40min and during three periods after this procedure: 0-5min, 5-10min and 10-15min. The CG had their heartbeats recorded at the same time; without receive massage. Cardiac autonomic modulation was investigated by heart rate variability (HRV). Results: The mean values of HRV rates were: pNN50, respectively, for the TG: before massage (10.5 ± 9.5%), and after massage: 0-5min (11.6 ± 7.2%), 5-10min (12.1 ± 8.0%) and 10-15min (11.1 ± 7.9%), with no significant statistical difference. The same result was found for the mean values of rMSSD index of the TG; before massage: 52.1 ± 46.2 ms, and after massage: 0-5min (50.0 ± 21.6ms), 5-10min (52.0 ± 27.4 ms) and 10-15min (48.2 ± 21.1 ms). Also, the values of LFnuand HFnu indexes did not change significantly before and after massage, and they were not statistically different from the values presented by the control group. Conclusion: The study results suggest that one session of classic massage does not modify cardiac autonomic modulation in healthy young adults.

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Digital Versus Traditional Workflow for Immediate Loading in Single-Implant Restoration: A Randomized Clinical Trial

Biology ◽

10.3390/biology10121281 ◽

2021 ◽

Vol 10 (12) ◽

pp. 1281

Author(s):

Paolo Capparé ◽

Francesco Ferrini ◽

Corrado Ruscica ◽

Giuseppe Pantaleo ◽

Giulia Tetè ◽

...

Keyword(s):

Controlled Trial ◽

Test Group ◽

Immediate Loading ◽

Control Group ◽

Marginal Bone Loss ◽

Digital Workflow ◽

Mean Values ◽

The Mean ◽

Implant Restoration

The purpose of this randomized controlled trial was to compare the immediate-loading protocol, in single restorations in the esthetic zone, by comparing the digital workflow in a test group (TG) vs. the analogical workflow in a control group (CG). A total of 50 patients were enrolled, requiring single hopeless tooth extraction. Twenty-five patients (TG) were randomly assigned to the immediate-loading protocol using the digital workflow, and twenty-five patients (CG) were assigned to the conventional workflow. Clinical and radiographic parameters were evaluated at the time of implant insertion (baseline) and after 3, 6 and 12 months, respectively. A clinician blind to conditions measured the Pink Esthetic Score (PES), as well as patient satisfaction. At 12-month follow-up, a cumulative survival rate of 100% was reported for all implants. No failures or biological complications were observed. No statistically significant differences were detected in the mean values of marginal bone loss and PES between the TG (0.12 ± 0.66 mm for MBL, 7.75 ± 0.89 for PES) and the CG (0.15 ± 0.54 mm for MBL, 7.50 ± 0.89 for PES). In 11 cases of TG, and 10 cases of CG, a one-year follow-up period showed an increased marginal bone level. No statistically significant differences were found in the mean total PES between test (7.75 ± 0.89) and control (7.5 ± 0.81) conditions. Furthermore, a customer satisfaction survey showed that patients preferred the digital workflow over the conventional workflow procedure (97.6 ± 4.3 vs. 69.2 ± 13.8). Digital workflow was more time-efficient than conventional workflow (97.2 ± 7.3 vs. 81.2 ± 11.3). Within the limitations of this study, no statistically significant differences were found between digital and traditional workflow.

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Protective Effect of Moringa Oleifera Leaves Against TramadolInduced Nephrotoxicity in Mice

INTERNATIONAL JOURNAL OF TOXICOLOGICAL AND PHARMACOLOGICAL RESEARCH ◽

10.25258/ijtpr.v9i02.9053 ◽

2017 ◽

Vol 9 (02) ◽

Author(s):

Ashraf Albrakati

Keyword(s):

Oral Dose ◽

Moringa Oleifera ◽

Treated Group ◽

Control Group ◽

Serum Urea ◽

Kidney Function Tests ◽

Mean Values ◽

Intraperitoneal Dose ◽

Moringa Oleifera Leaves ◽

The Mean

Tramadol, a broadly in recent years, is an effective analgesic agent for the treatment of moderate to acute pain. Its metabolites are excreted by the kidney which may cause nephrotoxicity. Moringa oleifera leaves are commonly used to provide herbal and plant-derived medicinal products especially in developing nations. The present study was carried out to determine the biochemical and histopathological changes in the kidney of tramadol-treated albino mice and to evaluate the possible protective role of Moringa oleifera leaves against tramadol-induced nephrotoxicity. Twenty adult albino mice were divided into four groups. Control group (group i) received daily intraperitoneal injection of normal saline only, group ii received oral dose of Moringa oleifera leaves extract (20 mg/kg/bw) for three weeks, group iii received daily intraperitoneal dose of tramadol (0.3 mg/kg/bw) for the same period, group iv, received daily oral dose of Moringa oleifera leaves extract, (20 mg/kg/bw) three hours before injecting intraperitoneal dose of tramadol (0.3 mg/kg/bw), for the same period. Blood samples were withdrawn at the end of the experiment for kidney function tests and specimens from the kidney were processed for histological study. No significant differences in the mean values of the kidney function tests were noticed between Moringa oleifera group and control group. However, there was highly significant increase in the mean values of serum, urea and creatinine in tramadol-treated group as compared to the control group. Although tramadol + Moringa oleifera group revealed significant difference in the mean values of urea and creatinine when compared with tramadol-treated group. So, Moringa oleifera leaves extract have been shown to attenuate the renal dysfunction, improve the renal architecture, with nearly normalization of serum urea and creatinine levels which indicate improvement of renal function. In conclusion, in the light of biochemical results and histological findings, co-administration of Moringa oleifera leaves lessened the negative effects of tramadol-induced nephrotoxicity; possibly by its antioxidant action. Further investigation of these promising protective effects of Moringa oleifera leaves against tramadol-induced renal injury may have considerable impact on developing an adjunct therapy aiming to improve the therapeutic index of some nephrotoxic drugs.

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Effect of Medialization of the Trochlear Groove and Lateralization of the Tibial Tubercle on TT-TG Distance: A Cross-sectional Study of Dysplastic and Nondysplastic Knees

The American Journal of Sports Medicine ◽

10.1177/0363546520987819 ◽

2021 ◽

pp. 036354652098781

Author(s):

Mathias Paiva ◽

Lars Blønd ◽

Per Hölmich ◽

Kristoffer Weisskirchner Barfod

Keyword(s):

Trochlear Dysplasia ◽

Cross Sectional Study ◽

Magnetic Resonance Images ◽

Tibial Tubercle ◽

Control Group ◽

Trochlear Groove ◽

Sectional Study ◽

Cross Sectional ◽

Mean Values ◽

The Mean

Background: Tibial tubercle–trochlear groove (TT-TG) distance is often used as a measure of lateralization of the TT and is important for surgical planning. Purpose: To investigate if increased TT-TG distance measured on axial magnetic resonance images is due to lateralization of the TT or medialization of the TG. Study Design: Cross-sectional study; Level of evidence, 3. Methods: A total of 84 knees (28 normal [NK], 28 with trochlear dysplasia [TD], and 28 with patellar dislocation without TD [PD]) were examined. The medial border of the posterior cruciate ligament (PCL) was chosen as the central anatomic landmark. The distance from the TT to PCL (TT-PCL) was measured to examine the lateralization of the TT. The distance from the TG to the PCL (TG-PCL) was measured to examine the medialization of the TG. Between-group differences were investigated by use of 1-way analysis of variance. Results: The mean values for TT-TG distance were 8.7 ± 3.6 mm for NK, 12.1 ± 6.0 mm for PD, and 16.7 ± 4.3 mm in the TD group ( P < .01). The mean values for TT-PCL distance were 18.5 ± 3.6 mm for NK, 18.5 ± 4.5 mm for PD, and 21.2 ± 4.2 mm in the TD group ( P = .03). The mean values for TG-PCL distance were 9.6 ± 3.0 mm for NK, 7.1 ± 3.4 mm for PD, and 5.1 ± 3.3 mm in the dysplastic group ( P < .01). Conclusion: The present results indicate that increased TT-TG distance is due to medialization of the TG and not lateralization of the TT. Knees with TD had increased TT-TG distance compared with the knees of the control group and the knees with PD. The TT-PCL distance did not differ significantly between groups, whereas the TG-PCL distance declined with increased TT-TG.

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Assessment of the Tissue Response to Modification of the Surface of Dental Implants with Carboxyethylphosphonic Acid and Basic Fibroblastic Growth Factor Immobilization (Fgf-2): An Experimental Study on Minipigs

Biology ◽

10.3390/biology10050358 ◽

2021 ◽

Vol 10 (5) ◽

pp. 358

Author(s):

Javier Aragoneses ◽

Ana Suárez ◽

Nansi López-Valverde ◽

Francisco Martínez-Martínez ◽

Juan Manuel Aragoneses

Keyword(s):

Surface Treatment ◽

Test Group ◽

Mean Value ◽

Control Group ◽

P Value ◽

Implant Surface ◽

Bone Contact ◽

Bone Implant ◽

Significant Difference ◽

The Mean

The aim of this study was to evaluate the effect of implant surface treatment with carboxyethylphosphonic acid and fibroblast growth factor 2 on the bone–implant interface during the osseointegration period in vivo using an animal model. The present research was carried out in six minipigs, in whose left tibia implants were inserted as follows: eight implants with a standard surface treatment, for the control group, and eight implants with a surface treatment of carboxyethylphosphonic acid and immobilization of FGF-2, for the test group. At 4 weeks after the insertion of the implants, the animals were sacrificed for the histomorphometric analysis of the samples. The means of the results for the implant–bone contact variable (BIC) were 46.39 ± 17.49% for the test group and 34.00 ± 9.92% for the control group; the difference was not statistically significant. For the corrected implant–bone contact variable (BICc), the mean value of the test group was 60.48 ± 18.11%, and that for the control group, 43.08 ± 10.77%; the difference was statistically significant (p-value = 0.035). The new bone formation (BV/TV) showed average results of 27.28 ± 3.88% for the test group and 26.63 ± 7.90% for the control group, meaning that the differences were not statistically significant (p-value = 0.839). Regarding the bone density at the interthread level (BAI/TA), the mean value of the test group was 32.27 ± 6.70%, and that of the control group was 32.91 ± 7.76%, with a p-value of 0.863, while for the peri-implant density (BAP/TA), the mean value of the test group was 44.96 ± 7.55%, and that for the control group was 44.80 ± 8.68%, without a significant difference between the groups. The current research only found a significant difference for the bone–implant contact at the cortical level; therefore, it could be considered that FGF-2 acts on the mineralization of bone tissue. The application of carboxyethylphosphonic acid on the surface of implants can be considered a promising alternative as a biomimetic coating for the immobilization of FGF-2. Despite no differences in the new bone formation around the implants or in the interthread or peri-implant bone density being detected, the biofunctionalization of the implant surface with FGF-2 accelerates the mineralization of the bone–implant interface at the cortical level, thereby reducing the osseointegration period.

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Bioassay of Chick Edema Factor—1962 Collaborative Study

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/46.3.406 ◽

1963 ◽

Vol 46 (3) ◽

pp. 406-412

Author(s):

D F Flick ◽

James Winbush ◽

Leo Friedman

Keyword(s):

Collaborative Study ◽

Test Group ◽

Negative Control Group ◽

Negative Control ◽

Pericardial Fluid ◽

Fluid Volume ◽

Control Group ◽

Fatty Material ◽

Edema Factor ◽

The Mean

Abstract The lower limits of sensitivity of the method by Douglass and Flick for the bioassay of the chick edema factor were more clearly delineated by studies this year in which toxic fatty material (TFM) was fed in duplicate trials at 0.00, 0.25, 0.50, 0.75, and 1.00 g per 16 g cottonseed oil, USP. Nine laboratories collaborated in these studies. Results indicated that the following criteria to establish presence of chick edema factor is valid: "t" value exceeds + 1.3, the mean log (pericardial fluid volume X 100) of the negative control group is 1.1460 or less, and the mean log (pericardial fluid volume X 100) of the test group is 1.1461 or more.

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Randomized controlled study comparing 2 surgical techniques for decompressive craniectomy: with watertight duraplasty and without watertight duraplasty

Journal of Neurosurgery ◽

10.3171/2017.4.jns152954 ◽

2018 ◽

Vol 129 (4) ◽

pp. 1017-1023 ◽

Cited By ~ 9

Author(s):

Eduardo Vieira ◽

Thiago C. Guimarães ◽

Igor V. Faquini ◽

Jose L. Silva ◽

Tammy Saboia ◽

...

Keyword(s):

Wound Infection ◽

Decompressive Craniectomy ◽

Surgical Complications ◽

Test Group ◽

Csf Leak ◽

Control Group ◽

Surgical Time ◽

Randomized Controlled ◽

The Mean ◽

Fluid Collections

OBJECTIVEDecompressive craniectomy (DC) is a widely used procedure in neurosurgery; however, few studies focus on the best surgical technique for the procedure. The authors’ objective was to conduct a prospective randomized controlled trial comparing 2 techniques for performing DC: with watertight duraplasty and without watertight duraplasty (rapid-closure DC).METHODSThe study population comprised patients ranging in age from 18 to 60 years who were admitted to the Neurotrauma Service of the Hospital da Restauração with a clinical indication for unilateral decompressive craniectomy. Patients were randomized by numbered envelopes into 2 groups: with watertight duraplasty (control group) and without watertight duraplasty (test group). After unilateral DC was completed, watertight duraplasty was performed in the control group, while in the test group, no watertight duraplasty was performed and the exposed parenchyma was covered with Surgicel and the remaining dura mater. Patients were then monitored daily from the date of surgery until hospital discharge or death. The primary end point was the incidence of surgical complications (CSF leak, wound infection, brain abscess, or subgaleal fluid collections). The following were analyzed as secondary end points: clinical outcome (analyzed using the Glasgow Outcome Scale [GOS]), surgical time, and hospital costs.RESULTSFifty-eight patients were enrolled, 29 in each group. Three patients were excluded, leaving 27 in the test group and 28 in the control group. There were no significant differences between groups regarding age, Glasgow Coma Scale score at the time of surgery, GOS score, and number of postoperative follow-up days. There were 9 surgical complications (5 in the control group and 4 in the test group), with no significant differences between the groups. The mean surgical time in the control group was 132 minutes, while in the test group the average surgical time was 101 minutes, a difference of 31 minutes (p = 0.001). The mean reduction in total cost was $420.00 USD (a 23.4% reduction) per procedure in the test group.CONCLUSIONSRapid-closure DC without watertight duraplasty is a safe procedure. It is not associated with a higher incidence of surgical complications (CSF leak, wound infection, brain abscess, or subgaleal fluid collections), and it decreased surgical time by 31 minutes on average. There was also a hospital cost reduction of $420.00 USD (23.4% reduction) per procedure.Clinical trial registration no.: NCT02594137 (clinicaltrials.gov)

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Association Between Osteoarticular Scores and Acute Phase Reactant Levels in Rheumatoid Arthritis

Journal of Medical Biochemistry ◽

10.2478/v10011-008-0031-2 ◽

2009 ◽

Vol 28 (2) ◽

pp. 116-121 ◽

Cited By ~ 1

Author(s):

Irena Kafeđiska ◽

Dejan Spasovski ◽

Todor Gruev ◽

Mane Grličkov ◽

Kočo Cakalaroski ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Acute Phase ◽

Acute Phase Reactant ◽

Control Group ◽

Time Intervals ◽

Acute Phase Reactants ◽

Mean Values ◽

Cell Counts ◽

Phase Reactant ◽

The Mean

Association Between Osteoarticular Scores and Acute Phase Reactant Levels in Rheumatoid Arthritis The aim of this prospective control study was a quantitative evaluation of the activity of rheumatoid arthritis (RA) in certain time intervals, using articular indexes (set of 28 sensitive and 28 swollen joints), laboratory parameters (Hb, Hct, Er, Le and Plt) and acute phase reactants (ESR, RF, CRP); to determine which of the acute phase reactants is the most useful biochemical marker for the evaluation of disease activity in RA; to quantify the therapeutical and laboratory differences in certain time intervals in the group with and without immunomodulatory therapy with Methotrexate. Sixty patients with RA were included, 27 of who were treated with non-steroid antiinflammatory drugs (NSAIDs) and Methotrexate (MTX). The control group consisted of 33 patients treated only with NSAIDs because of irregular controls. In the first group of patients the disease activity was estimated at four time intervals, and in the control group of patients at three time intervals following the scores of the articular indexes, blood cell counts, ESR and CRP in every patient. In the first group of patients decreased activity of RA was found upon every following control with a consecutive decrease in mean values of the scores of articular indexes with statistically significant differences at the four time intervals. Considering laboratory parameters, there were statistically significant differences in the mean values of Hb, Er, Plt, ESR, (p=0.0462, p=0.0076, p= 0.0058, p= 0.0003). Mean values of CRP did not show statistically significant differences, but the number of patients who were CRP negative increased (there were great standard deviations). In the group of patients treated only with NSAIDs, there were statistically significant differences in the mean values of the scores of articular indexes with an increse at every following control (in favour of progression of the disease). There were no statistically significant differences considering blood cell counts, ESR and CRP (in favour of permanently active disease). In conclusion, CRP is the most useful marker for the prospective follow-up of patients with RA.

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Effects of Passive Immunization by Anti-Gingipain IgY on the Oral Health of Cats Fed Kibble Diets

Journal of Veterinary Dentistry ◽

10.1177/0898756418814010 ◽

2018 ◽

Vol 35 (4) ◽

pp. 275-280 ◽

Cited By ~ 1

Author(s):

Patrícia Massae Oba ◽

Fernanda Corrêa Devito ◽

João Paulo Fernandes Santos ◽

Rafael Nóbrega Stipp ◽

Márcia de Oliveira Sampaio Gomes ◽

...

Keyword(s):

Oral Health ◽

Adverse Reactions ◽

Passive Immunization ◽

Test Group ◽

Control Group ◽

Promising Alternative ◽

Mixed Breed ◽

Prevalent Disease ◽

Adult Cats ◽

Key Microorganisms

Porphyromonas gulae is one of the key microorganisms in biofilm dysbiosis that leads to periodontal disease, a prevalent disease in cats. Gingipains are proteases secreted that promote the disruption of cell adhesion and the differentiation of osteoclasts. The use of anti-gingipain immunoglobulin Y (IgY-GP) has emerged as a promising alternative to conventional prevention and treatment methods. The aim of this study was to evaluate the efficacy of IgY-GP on different parameters related to oral health in cats. Twenty adult mixed-breed cats were divided into 2 groups of 10 cats fed with 2 extruded diets differing only by coating. The control group received a coating of 4 g/kg of powdered egg and the test group received a coating of 4 g/kg of IgY-GP. The experiment followed a crossover design with 2 periods, each lasting 40 days with a washout period of 30 days. The evaluated parameters were plaque, calculus, gingivitis index, and percentage of Porphyromonas gingivalis of the oral cavity (%PG-OC) at baseline and after 40 days of diet consumption. All cats remained healthy throughout the study and no adverse reactions or side effects were observed. After 40 days of IgY-GP consumption, the plaque index was significantly lower compared to the baseline ( P = .0133). There were no significant changes in calculus index, gingivitis index, and %PG-OC between groups after 40 days ( P > .05). The consumption of IgY-GP reduces plaque accumulation, which may lead to an improvement in the oral health of adult cats.

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