scholarly journals A study on Antioxidant Properties of Different Extracts from Kitaibelia balansae

Proceedings ◽  
2019 ◽  
Vol 40 (1) ◽  
pp. 23
Author(s):  
Zengin ◽  
Aygun ◽  
Aktumsek
Keyword(s):  
Ethyl Acetate ◽  
Human Body ◽  
Antioxidant Properties ◽  
Water Extract ◽  
Antioxidant Ability ◽  
Chemical Methods ◽  
Potential Source ◽  
First Time ◽  
Antioxidant Effects ◽  
Ethyl Acetate Extracts

Nowadays, knowledge of ancient botanical medicinal practices and application of modern phytochemical techniques have provided the excellent tools for the purification and structural elucidation of various phyto-compounds, which, in turn, has given insights into their mode of action on the human body. This study has been designed to investigate for the first time the antioxidant effects of the ethyl acetate, methanolic, and water extracts of Kitaibelia balansae. Different chemical methods were performed and the observed abilities depend on the solvent used. The best antioxidant ability was noted in water extract, followed by methanol and ethyl acetate extracts. The highest level of phenolic was also detected in water extract. The present findings suggest that K. balansae can be considered as a potential source of bioactive compounds for novel phytopharmaceuticals development

scholarly journals Antioxidant Effects of Glechoma hederacea as a Food Additive

2010 ◽  
Vol 5 (1) ◽  
pp. 1934578X1000500
Author(s):  
Mirjana Milovanovic ◽  
Dusan Zivkovic ◽  
Biljana Vucelic-Radovic
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antioxidant Properties ◽  
Water Extract ◽  
Storage Conditions ◽  
Strong Concentration ◽  
Rancimat Method ◽  
Prime Steam ◽  
Glechoma Hederacea ◽  
Ethyl Acetate Extracts

The antioxidant properties of Glechoma hederacea L. (Lamiaceae), of Serbian origin, were studied in respect to its potential use in foodstuffs. Ethanol-water (8:2, v/v) and purified ethyl acetate extracts of the plant were found to possess significant antioxidant activity. Tests were performed on two different substrates, prime steam pork lard and active-carbon-treated edible sunflower oil, using Schaal oven test storage conditions at 60°C. The ethanol-water and purified ethyl acetate extracts of G. hederacea showed strong concentration-dependent antioxidant activity. On the contrary, under the Rancimat method conditions at 120°C, the ethanol-water extract showed significantly stronger antioxidant activity, in comparison with the other tested extracts. All activities were compared with commercial antioxidants, such as BHA and a tocopherol mixture, respectively. For the first time, the activity of the flavonol quercetagetin was determined.

scholarly journals In vitro Antidiabetic, anti-obesity and antioxidant proprities of Rosemary extracts

2014 ◽  
Vol 10 (2) ◽  
pp. 2305-2316 ◽  
Author(s):  
Manel Ben Ali ◽  
Kais Mnafgui ◽  
Abdelfattah Feki ◽  
Mohamed Damak ◽  
Noureddine Allouche
Keyword(s):  
Essential Oil ◽  
Ethyl Acetate ◽  
Pancreatic Lipase ◽  
Antioxidant Properties ◽  
Methanolic Extracts ◽  
Ic50 Values ◽  
Inhibitory Potential ◽  
Prevention Of Diabetes ◽  
First Time

Diabetes mellitus is a serious health problem worldwide that has adverse and long-lasting consequences for individuals, families, and communities. Hence, this study sought to investigate the inhibitory potential of rosemary extracts on key-enzymes related to diabetes such as α-amylase and pancreatic lipase activities, as well as to assess their antioxidant properties in vitro. The IC50 values of Rosemary essential oil, ethyl acetate and methanolic extracts against α-amylase were 28.36, 34.11 and 30.39 µg/mL respectively, and those against pancreatic lipase were 32.25, 36.64 and 34.07 µg/mL, suggesting strong anti-diabetic and anti-obesity effects of Rosemary. The methanolic extract was found to be the highest in levels of phenolic (282.98 µgGAE/mg extract) and flavonoids (161.05 µg QE /mg extract) contents as well as in the antioxidant activity (IC50 = 15.82 µg/mL) as compared to other extracts ethyl acetate (IC50 = 32.23 µg/mL) and essential oil  (IC50 = 96.12 µg/mL).Antioxidant efficacy of Rosemary extracts has been estimated in the stabilization of sunflower oil (SFO) at three different concentrations, i.e. 200 (SFO-200), 500 (SFO-500) and 1000 ppm (SFO-1000). Results showed the highest efficiency of SFO-1000.The results obtained in this study demonstrated for the first time that Rosemary is a potent source of natural inhibitors of α-amylase and pancreatic lipase with powerful antioxidants proprieties that might be used in the food stabilization and the prevention of diabetes and obesity complications as a complementary pharmacological drug.

Ethyl acetate fraction of Cymbopogon citratus as a potential source of antioxidant compounds

2018 ◽  
Vol 42 (5) ◽  
pp. 3642-3652 ◽  
Author(s):  
Ana Carolina Mendes Hacke ◽  
Jacqueline Aparecida Marques ◽  
José Carlos Rebuglio Vellosa ◽  
Aline Augusti Boligon ◽  
Fernanda D’Avila da Silva ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Aqueous Phase ◽  
Antioxidant Properties ◽  
Ethyl Acetate Fraction ◽  
Antioxidant Compounds ◽  
Cymbopogon Citratus ◽  
Potential Source

New fraction from a lemongrass acidic aqueous phase as a source of compounds with remarkable antioxidant properties by different in vitro mechanisms.

Anasterocerebroside A, a New Glucosylceramide from the Patagonian Starfish Anasterias minuta

2003 ◽  
Vol 58 (5-6) ◽  
pp. 433-440 ◽  
Author(s):  
Hugo D. Chludil ◽  
Alicia M. Seldes ◽  
Marta S. Maier
Keyword(s):  
Methylene Chloride ◽  
Lipid Fraction ◽  
Water Extract ◽  
Pure Compound ◽  
Chemical Methods ◽  
First Time

Abstract Eight glucosylceramides (1-8) were isolated from the water-insoluble lipid fraction of a methylene chloride/methanol/water extract of the Patagonian starfish Anasterias minuta. One of the constituents was identified as a new glucosylceramide, anasterocerebroside A (1), while the known glucosylceramide 7 was isolated and characterized for the first time as a pure compound. The structures of 1 and 7 were established by spectroscopic and chemical methods.

scholarly journals Characterization and Antioxidant Properties of Six Algerian Propolis Extracts: Ethyl Acetate Extracts Inhibit Myeloperoxidase Activity

2014 ◽  
Vol 15 (2) ◽  
pp. 2327-2345 ◽  
Author(s):  
Yasmina Boufadi ◽  
Jalal Soubhye ◽  
Ali Riazi ◽  
Alexandre Rousseau ◽  
Michel Vanhaeverbeek ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Properties ◽  
Myeloperoxidase Activity ◽  
Ethyl Acetate Extracts

scholarly journals Studies on the Dual Cytotoxicity and Antioxidant Properties of Berberis vulgaris Extracts and Its Main Constituent Berberine

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Lamyae El khalki ◽  
Mounir Tilaoui ◽  
Abdeslam Jaafari ◽  
Hassan Ait Mouse ◽  
Abdelmajid Zyad
Keyword(s):  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Cytotoxic Effect ◽  
Antioxidant Properties ◽  
Ethanol Extract ◽  
Antagonistic Effect ◽  
Breast Adenocarcinoma ◽  
No Donor ◽  
Berberis Vulgaris ◽  
Ethyl Acetate Extracts

The present study attempts to investigate the cytotoxic activity of ethanol and ethyl acetate extracts of the Moroccan Berberis vulgaris and its major component berberine, together with exploring their antioxidant properties. It also consists of studying the combination effect of berberine and S-nitroso-N-acetylpenicillamine (SNAP), a nitric oxide (NO) donor, against the human breast adenocarcinoma cell line (MCF-7). Using the MTT assay, we report a differential cytotoxic effect of ethanol and ethyl acetate extracts since the ethanol extract is more cytotoxic than the ethyl acetate one, with IC50 = 3.54 μg/mL and 596.71 μg/mL, respectively. Interestingly, no cytotoxic effect was observed against normal cells. Furthermore, these extracts showed a remarkable antioxidant activity as measured by the DPPH free radicals scavenging assay. In fact, the IC50 values are 69.65 μg/mL and 77.75 μg/mL for the ethanol and ethyl acetate extracts, respectively. In addition, several concentrations of berberine, when combined with the NO donor used at IC30, induced a synergistic cytotoxic activity at concentrations ranging from 8.40 μM to 33.60 μM, as revealed by the combination index values, using the Chou–Talalay method. However, at the other concentrations tested, an antagonistic effect was observed. The observed cytotoxicity was related to apoptosis induction as demonstrated by the annexin-V-streptavidin FITC-staining analysis.

scholarly journals Antioxidant and Cytotoxic Activities of Usnea barbata (L.) F.H. Wigg. Dry Extracts in Different Solvents

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 909
Author(s):  
Violeta Popovici ◽  
Laura Bucur ◽  
Antoanela Popescu ◽  
Verginica Schröder ◽  
Teodor Costache ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Acid Content ◽  
Antioxidant Properties ◽  
Usnic Acid ◽  
Gravimetric Method ◽  
Water Extract ◽  
Antioxidant Effect ◽  
Cytotoxic Activities ◽  
Therapeutic Effects ◽  
Total Polyphenols

Lichens represent a significant source of antioxidants due to numerous metabolites that can reduce free radicals. Usnea barbata (L.) F.H. Wigg. has been recognized and used since ancient times for its therapeutic effects, some of which are based on its antioxidant properties. The present study aims to analyze the phytochemical profile and to evaluate the antioxidant and cytotoxic potential of this lichen species. Five dry extracts of U. barbata (UBDE) in different solvents (acetone, ethyl acetate, ethanol, methanol, water) were prepared by refluxing at Soxhlet to achieve these proposed objectives and to identify which solvent is the most effective for the extraction. The usnic acid content (UAC) was quantified by ultra-high performance liquid chromatography (UHPLC). The total polyphenols content (TPC) and tannins content (TC) were evaluated by spectrophotometry, and the total polysaccharides (PSC) were extracted by a gravimetric method. The 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) free radical method was used to assess the antioxidant activity (AA) and the Brine Shrimp Lethality (BSL) assay was the biotest for cytotoxic activity evaluation. The ethyl acetate extract had the highest usnic acid content, and acetone extract had the highest content of total polyphenols and tannins. The most significant antioxidant effect was reported to methanol extract, and all the extracts proved high cytotoxicity. The water extract has the lowest cytotoxicity because usnic acid is slightly soluble in this solvent, and it was not found at UHPLC analysis. All extracts recorded a moderate correlation between the content of usnic acid, polyphenols, tannins, and AA; furthermore, it has been observed that the cytotoxicity varies inversely with the antioxidant effect.

scholarly journals The phytochemical investigation from n-hexane extract of the lichen Roccella montagnei

2021 ◽  
Vol 24 (2) ◽  
pp. first
Author(s):  
Thu Thi-Hoai Nguyen ◽  
Duong Thuc Huy
Keyword(s):  
Ethyl Acetate ◽  
Hexane Extract ◽  
Published Data ◽  
Chemical Structures ◽  
Nmr Data ◽  
Phytochemical Investigation ◽  
Partition Method ◽  
First Time ◽  
Ethyl Acetate Extracts

Introduction: R occella montagnei is widely distributed in subtropical regions. As the continuous study on the hexane extract of Roccella montagnei lichen, the isolation and structural determination of five compounds were addressed. Method: The crude extract was obtained from the dried lichen powder's extraction at room temperature. The n-hexane, n-hexane-ethyl acetate, and ethyl acetate extracts were obtained by the liquid-liquid partition method. The organic compounds were isolated from n-hexane extract by silica gel and Sephadex LH-20 column chromatography. Their chemical structures were identified by the NMR and HR-ESI-MS data analysis and the comparison of their NMR data with the published data. Results: Five compounds were isolated and chemically structural identified, consisting of 3b -hydroxy-7a-methoxystigmast-5-ene (1), sekikaic acid (2), lichenxanthone (3), (+)-6,8-dihydroxy-3-propyl-3,4-dihydroisocoumarin (4), and ar-turmerone (5). Conclusion: To the best of our knowledge, except 3 which was reported from this species for the first time, four isolated compounds left did not known to be present in Roccella genus before.

scholarly journals In vitro Antioxidant Properties of Bersama abyssinica Stem Bark Extracts

Proceedings ◽  
2019 ◽  
Vol 40 (1) ◽  
pp. 21
Author(s):  
Kouadio Ibrahime Sinan ◽  
Kouadio Bene ◽  
Abdurrahman Aktumsek ◽  
Gokhan Zengin
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Water Extract ◽  
Reducing Power ◽  
Stem Bark ◽  
Sub Saharan Africa ◽  
Total Phenolic ◽  
Bersama Abyssinica

Bersama abyssinica, belonging to the Melianthaceae family, is distributed across Sub Saharan Africa. Decoctions of the bark, leaves, and roots of B. abyssinica have been extensively used in traditional medicine to manage many stomach complications such as colic, diarrhea, dysentery, and intestinal worms. In this study, we examined three extracts (ethyl acetate, methanol and water) obtained from B. abyssinica stem barks in terms of antioxidant properties. The antioxidant abilities were investigated by different chemical methods, including free radical scavenging (DPPH and ABTS), reducing power (CUPRAC and FRAP), metal chelating and phosphomolybdenum assay. In addition, total phenolic and flavonoid contents in the extracts were calculated. The highest level of phenolics was determined in water extract (230.83 mg GAE/g extract), followed by methanol (216.79 mg GAE/g extract) and ethyl acetate (100.57 mg GAE/g extract). In same line with total phenolic content, the best antioxidant properties were noted for water and methanol extracts. Our findings suggested that B. abyssinica stem bark extracts could be considered as promising sources of natural antioxidants.

scholarly journals Biopotential of Bersama abyssinica Fresen Stem Bark Extracts: UHPLC Profiles, Antioxidant, Enzyme Inhibitory, and Antiproliferative Propensities

Antioxidants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 163 ◽  
Author(s):  
Kouadio Ibrahime Sinan ◽  
Annalisa Chiavaroli ◽  
Giustino Orlando ◽  
Kouadio Bene ◽  
Gokhan Zengin ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Hct116 Cell ◽  
Antioxidant Properties ◽  
Water Extract ◽  
Stem Bark ◽  
Scientific Data ◽  
Water Extracts ◽  
Bersama Abyssinica

In this study, ethyl acetate, methanol, and water extracts of Bersama abyssinica (Melianthaceae) stem bark were screened for enzyme inhibitory and antioxidant properties. The water extract possessed the highest concentration of phenols (230.83 mg gallic acid equivalent/g extract), while the methanol extract was rich in flavonoids (75.82 mg rutin equivalent/g extract), and the ethyl acetate extract possessed the highest amount of saponins (97.37 mg quillaja equivalent/g). The aim of this study was to investigate the antiproliferative effects against the human colon cancer HCT116 cell line challenged with serotonin (5-HT) as a stimulating-proliferation factor. The level of HCT116 cell-deriving pool of kynurenic acid (KA) was also assessed. The UHPLC results confirmed the presence of 58, 68, and 63 compounds in the ethyl acetate, methanol, and water extracts, respectively. Mangiferin, vitexin and its isomer isovitexin were tentatively identified in all extracts and KA (m/z 190.05042 [M−H]+) was also tentatively identified in the methanol and water extracts. The methanol extract (1464.08 mg Trolox equivalent [TE]/g extract) showed the highest activity in the CUPRAC assay, whereas the water extract (1063.70 mg TE/g extract) showed the highest activity with the FRAP technique. The ethyl acetate extract was the most active acetylcholinesterase (4.43 mg galantamine equivalent/g extract) and α-glucosidase (mmol acarbose equivalent /g extract) inhibitor. The water extract was able to inhibit 5-HT-stimulated viability of HCT116 cells, and blunt 5-HT-induced reduction of cell-deriving KA. The scientific data generated in this study provide baseline data regarding the biological properties of B. abyssinica stem bark, highlighting its potential use for the development of new pharmaceutic and cosmetic agents.

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