scholarly journals Characterization of Recombinant His-Tag Protein Immobilized onto Functionalized Gold Nanoparticles

Sensors ◽  
2018 ◽  
Vol 18 (12) ◽  
pp. 4262 ◽  
Author(s):  
Lisa Torres-González ◽  
Ramonita Díaz-Ayala ◽  
Carmen Vega-Olivencia ◽  
Juan López-Garriga
Keyword(s):  
Gold Nanoparticles ◽  
Photoelectron Spectroscopy ◽  
Attenuated Total Reflection ◽  
Current Response ◽  
Nickel Ion ◽  
Lucina Pectinata ◽  
His Tag ◽  
Α Helix ◽  
Surface Modified

The recombinant polyhistidine-tagged hemoglobin I ((His)6-rHbI) from the bivalve Lucina pectinata is an ideal biocomponent for a hydrogen sulfide (H2S) biosensor due to its high affinity for H2S. In this work, we immobilized (His)6-rHbI over a surface modified with gold nanoparticles functionalized with 3-mercaptopropionic acid complexed with nickel ion. The attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) analysis of the modified-gold electrode displays amide I and amide II bands characteristic of a primarily α-helix structure verifying the presence of (His)6-rHbI on the electrode surface. Also, X-ray photoelectron spectroscopy (XPS) results show a new peak after protein interaction corresponding to nitrogen and a calculated overlayer thickness of 5.3 nm. The functionality of the immobilized hemoprotein was established by direct current potential amperometry, using H2S as the analyte, validating its activity after immobilization. The current response to H2S concentrations was monitored over time giving a linear relationship from 30 to 700 nM with a corresponding sensitivity of 3.22 × 10−3 nA/nM. These results confirm that the analyzed gold nanostructured platform provides an efficient and strong link for polyhistidine-tag protein immobilization over gold and glassy carbon surfaces for a future biosensors development.

The Internal Structure of Macroporous Membranes and Transport of Surface-Modified Nanoparticles

2015 ◽  
Vol 21 (4) ◽  
pp. 936-945 ◽  
Author(s):  
Sang J. Lee ◽  
Kiwoong Kim ◽  
Sungsook Ahn
Keyword(s):  
Gold Nanoparticles ◽  
Internal Structure ◽  
Morphological Structure ◽  
Improve Performance ◽  
X Ray ◽  
X Ray Scattering ◽  
Cellulose Acetate Membranes ◽  
Surface Modified ◽  
Surface Modified Nanoparticles

AbstractUnderstanding the morphological structure of membranes is essential to improve performance of membrane-based applications. In this paper, macroporous membranes were investigated and two methods introduced as an alternative for characterization of stereo-structure of the membranes. We combined the use of synchrotron X-ray nanotomography and small-angle X-ray scattering to examine the internal structure of cellulose acetate membranes with studies of the capture of surface-modified gold nanoparticles within these membranes. Finally, the morphological structures of macroporous membranes were visualized and their relationships with penetration tendency of surface-modified gold nanoparticles were explained.

Surface Characterization of Poly(acrylic acid) Grafted to Photo-oxidized Perfluorosulfonic Acid Membrane Used in Fuel Cells

2010 ◽  
Vol 1269 ◽  
Author(s):  
Alla Bailey ◽  
Fei Lu ◽  
Ameya Khot ◽  
Shahida Hussain ◽  
Kyle W. Rugg ◽  
...  
Keyword(s):  
Acrylic Acid ◽  
Photoelectron Spectroscopy ◽  
Surface Characterization ◽  
Microwave Plasma ◽  
Perfluorosulfonic Acid ◽  
Photo Oxidation ◽  
Modified Surface ◽  
Surface Modified ◽  
Poly Acrylic Acid

AbstractPerfluorosulfonic acid membrane (Nafion®-117) was first surface modified with atmospheric pressure UV photo-oxidation or low-pressure vacuum UV photo-oxidation downstream from an Ar microwave plasma, and then graft polymerized with acrylic acid. X-ray photoelectron spectroscopy (XPS) was used to analyze the modified Nafion surface and poly(acrylic acid) grafted to the modified surface.

scholarly journals X-ray photoelectron spectroscopy characterization of gold nanoparticles functionalized with amine-terminated alkanethiols

2011 ◽  
Vol 6 (3) ◽  
pp. 98-104 ◽  
Author(s):  
Sirnegeda D. Techane ◽  
Lara J. Gamble ◽  
David G. Castner
Keyword(s):  
Gold Nanoparticles ◽  
Photoelectron Spectroscopy ◽  
X Ray

scholarly journals Structure and Oligonucleotide Binding Efficiency of Differently Prepared Click Chemistry-Type DNA Microarray Slides Based on 3-Azidopropyltrimethoxysilane

Materials ◽  
2021 ◽  
Vol 14 (11) ◽  
pp. 2855
Author(s):  
Emilia Frydrych-Tomczak ◽  
Tomasz Ratajczak ◽  
Łukasz Kościński ◽  
Agnieszka Ranecka ◽  
Natalia Michalak ◽  
...  
Keyword(s):  
Photoelectron Spectroscopy ◽  
Dna Microarrays ◽  
Structural Characteristics ◽  
X Ray ◽  
Force Microscopy ◽  
Atomic Force ◽  
Glass Slides ◽  
Surface Modified ◽  
Oligonucleotide Binding

The structural characterization of glass slides surface-modified with 3-azidopropyltrimethoxysilane and used for anchoring nucleic acids, resulting in the so-called DNA microarrays, is presented. Depending on the silanization conditions, the slides were found to show different oligonucleotide binding efficiency, thus, an attempt was made to correlate this efficiency with the structural characteristics of the silane layers. Atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and X-ray reflectometry (XRR) measurements provided information on the surface topography, chemical composition and thickness of the silane films, respectively. The surface for which the best oligonucleotides binding efficiency is observed, has been found to consist of a densely-packed silane layer, decorated with a high-number of additional clusters that are believed to host exposed azide groups.

scholarly journals Revealing the Active Site of Gold Nanoparticles for the Peroxidase-Like Activity: The Determination of Surface Accessibility

Catalysts ◽  
2019 ◽  
Vol 9 (6) ◽  
pp. 517 ◽  
Author(s):  
Ching-Ping Liu ◽  
Kuan-Chung Chen ◽  
Ching-Feng Su ◽  
Po-Yen Yu ◽  
Po-Wei Lee
Keyword(s):  
Gold Nanoparticles ◽  
Active Site ◽  
Photoelectron Spectroscopy ◽  
Active Sites ◽  
Inhibition Mechanism ◽  
Surface Accessibility ◽  
Peroxidase Mimics ◽  
Catalytic Active Sites ◽  
Surface Modified ◽  
First Time

Despite the fact that the enzyme-like activities of nanozymes (i.e., nanomaterial-based artificial enzymes) are highly associated with their surface properties, little is known about the catalytic active sites. Here, we used the sulfide ion (S2−)-induced inhibition of peroxidase-like activity to explore active sites of gold nanoparticles (AuNPs). The inhibition mechanism was based on the interaction with Au(I) to form Au2S, implying that the Au(I) might be the active site of AuNPs for the peroxidase-like activity. X-ray photoelectron spectroscopy (XPS) analysis showed that the content of Au(I) on the surface of AuNPs significantly decreased after the addition of S2−, which might be contributed to the more covalent Au–S bond in the formation of Au2S. Importantly, the variations of Au(I) with and without the addition of S2− for different surface-capped AuNPs were in good accordance with their corresponding peroxidase-like activities. These results confirmed that the accessible Au(I) on the surface was the main requisite for the peroxidase-like activity of AuNPs for the first time. In addition, the use of S2− could assist to determine available active sites for different surface modified AuNPs. This work not only provides a new method to evaluate the surface accessibility of colloidal AuNPs but also gains insight on the design of efficient AuNP-based peroxidase mimics.

AQUEOUS-BASED FORMATION OF GOLD NANOPARTICLES ON SURFACE-MODIFIED COTTON TEXTILES

2013 ◽  
Vol 01 (01) ◽  
pp. 1250001 ◽  
Author(s):  
HONGJUN LIU ◽  
WEI-PENG GOH ◽  
TYLER B. NORSTEN
Keyword(s):  
Gold Nanoparticles ◽  
Surface Treatment ◽  
Photoelectron Spectroscopy ◽  
Fiber Surface ◽  
Vinyl Pyrrolidone ◽  
Cotton Fibers ◽  
Au Nps ◽  
X Ray ◽  
Cotton Textiles ◽  
Surface Modified

A facile and environmental friendly route has been developed to synthesize gold nanoparticles (AuNPs) by using amine stabilizers and N-vinyl pyrrolidone (NVP) as the reductant in an aqueous medium. This synthetic concept can be applied to the formation of Au NPs on normal cotton fibers via in situ surface modification of the cotton fibers by (3-aminopropyl)triethoxysilane (ATS) followed by nanoparticle formation. This scheme produces red Au NP-dyed cotton textiles. Surface treatment of the cotton textiles by ATS was found to be crucial for the formation of nano- Au . X-ray photoelectron spectroscopy (XPS), diffused reflectance UV-visible spectroscopy (UV-vis) and energy dispersive X-ray (EDX) were used to investigate the fiber surfaces. The distribution of Au NPs as well as surface treatment agent on the fiber surface was located by time of flight secondary ion mass spectroscopy (TOF-SIMS).

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