scholarly journals Remediation of Petroleum-Contaminated Soils with Microbial and Microbial Combined Methods: Advances, Mechanisms, and Challenges

2021 ◽  
Vol 13 (16) ◽  
pp. 9267
Author(s):  
Xin Sui ◽  
Xuemei Wang ◽  
Yuhuan Li ◽  
Hongbing Ji
Keyword(s):  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Low Cost ◽  
Hydrocarbon Contamination ◽  
Environmental Consequences ◽  
Petroleum Hydrocarbon Contamination ◽  
Remediation Strategies ◽  
Microbial Remediation ◽  
Microbiological Techniques ◽  
Petroleum Pollutants

The petroleum industry’s development has been supported by the demand for petroleum and its by-products. During extraction and transportation, however, oil will leak into the soil, destroying the structure and quality of the soil and even harming the health of plants and humans. Scientists are researching and developing remediation techniques to repair and re-control the afflicted environment due to the health risks and social implications of petroleum hydrocarbon contamination. Remediation of soil contamination produced by petroleum hydrocarbons, on the other hand, is a difficult and time-consuming job. Microbial remediation is a focus for soil remediation because of its convenience of use, lack of secondary contamination, and low cost. This review lists the types and capacities of microorganisms that have been investigated to degrade petroleum hydrocarbons. However, investigations have revealed that a single microbial remediation faces difficulties, such as inconsistent remediation effects and substantial environmental consequences. It is necessary to understand the composition and source of pollutants, the metabolic genes and pathways of microbial degradation of petroleum pollutants, and the internal and external aspects that influence remediation in order to select the optimal remediation treatment strategy. This review compares the degradation abilities of microbial–physical, chemical, and other combination remediation methods, and highlights the degradation capabilities and processes of the greatest microbe-biochar, microbe–nutrition, and microbe–plant technologies. This helps in evaluating and forecasting the chemical behavior of contaminants with both short- and long-term consequences. Although there are integrated remediation strategies for the removal of petroleum hydrocarbons, practical remediation remains difficult. The sources and quantities of petroleum pollutants, as well as their impacts on soil, plants, and humans, are discussed in this article. Following that, the focus shifted to the microbiological technique of degrading petroleum pollutants and the mechanism of the combined microbial method. Finally, the limitations of existing integrated microbiological techniques are highlighted.

scholarly journals Petroleum Hydrocarbon Contamination in Terrestrial Ecosystems—Fate and Microbial Responses

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3400 ◽  
Author(s):  
Adam Truskewycz ◽  
Taylor D. Gundry ◽  
Leadin S. Khudur ◽  
Adam Kolobaric ◽  
Mohamed Taha ◽  
...  
Keyword(s):  
Natural Attenuation ◽  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Petroleum Hydrocarbon ◽  
Terrestrial Ecosystems ◽  
Hydrocarbon Contamination ◽  
Microbial Consortia ◽  
Metagenomic Sequencing ◽  
Petroleum Hydrocarbon Contamination ◽  
The Impact

Petroleum hydrocarbons represent the most frequent environmental contaminant. The introduction of petroleum hydrocarbons into a pristine environment immediately changes the nature of that environment, resulting in reduced ecosystem functionality. Natural attenuation represents the single, most important biological process which removes petroleum hydrocarbons from the environment. It is a process where microorganisms present at the site degrade the organic contaminants without the input of external bioremediation enhancers (i.e., electron donors, electron acceptors, other microorganisms or nutrients). So successful is this natural attenuation process that in environmental biotechnology, bioremediation has developed steadily over the past 50 years based on this natural biodegradation process. Bioremediation is recognized as the most environmentally friendly remediation approach for the removal of petroleum hydrocarbons from an environment as it does not require intensive chemical, mechanical, and costly interventions. However, it is under-utilized as a commercial remediation strategy due to incomplete hydrocarbon catabolism and lengthy remediation times when compared with rival technologies. This review aims to describe the fate of petroleum hydrocarbons in the environment and discuss their interactions with abiotic and biotic components of the environment under both aerobic and anaerobic conditions. Furthermore, the mechanisms for dealing with petroleum hydrocarbon contamination in the environment will be examined. When petroleum hydrocarbons contaminate land, they start to interact with its surrounding, including physical (dispersion), physiochemical (evaporation, dissolution, sorption), chemical (photo-oxidation, auto-oxidation), and biological (plant and microbial catabolism of hydrocarbons) interactions. As microorganism (including bacteria and fungi) play an important role in the degradation of petroleum hydrocarbons, investigations into the microbial communities within contaminated soils is essential for any bioremediation project. This review highlights the fate of petroleum hydrocarbons in tertial environments, as well as the contributions of different microbial consortia for optimum petroleum hydrocarbon bioremediation potential. The impact of high-throughput metagenomic sequencing in determining the underlying degradation mechanisms is also discussed. This knowledge will aid the development of more efficient, cost-effective commercial bioremediation technologies.

scholarly journals Metagenomic Characteristics of Bacterial Response to Petroleum Hydrocarbon Contamination in Diverse Environments as Revealed by Functional Taxonomic Strategies

2016 ◽  
Author(s):  
Arghya Mukherjee ◽  
Bobby Chettri ◽  
James S. Langpoklakpam ◽  
Pijush Basak ◽  
Aravind Prasad ◽  
...  
Keyword(s):  
Petroleum Hydrocarbons ◽  
Petroleum Hydrocarbon ◽  
High Throughput Sequencing ◽  
Hydrocarbon Contamination ◽  
Bioinformatic Tools ◽  
Bacterial Response ◽  
Petroleum Hydrocarbon Contamination ◽  
Microbial Remediation ◽  
Recent Developments ◽  
Polluted Sites

AbstractMicrobial remediation of oil polluted habitats remains one of the foremost methods for restoration of petroleum hydrocarbon contaminated environments. The development of effective bioremediation strategies however, require an extensive understanding of the resident microbiome of these habitats. Recent developments such as high-throughput sequencing has greatly facilitated the advancement of microbial ecological studies in oil polluted habitats. However, effective interpretation of biological characteristics from these large datasets remains a considerable challenge. In this study, we have implemented recently developed bioinformatic tools for analyzing 65 publicly available 16S rRNA datasets from 12 diverse hydrocarbon polluted habitats to decipher metagenomic characteristics of bacterial communities of the same. We have comprehensively described phylogenetic and functional compositions of these habitats and additionally inferred a multitude of metagenomic features including 255 taxa and 414 functional modules which can be used as biomarkers for effective distinction between the 12 oil polluted sites. We have identified essential metabolic signatures and also showed that significantly over-represented taxa often contribute to either or both, hydrocarbon degradation and additional important functions. Our findings reveal significant differences between hydrocarbon contaminated sites and establishes the importance of endemic factors in addition to petroleum hydrocarbons as driving factors for sculpting hydrocarbon contaminated bacteriomes.

The Remedial Effect of the Decomposing Bacteria on Different Petroleum Hydrocarbon Contamination

2011 ◽  
Vol 414 ◽  
pp. 88-92
Author(s):  
Xiao Nan Sun ◽  
An Ping Liu ◽  
Wen Ting Sun ◽  
Shu Chang Jin
Keyword(s):  
Growth Curve ◽  
Petroleum Hydrocarbons ◽  
Petroleum Hydrocarbon ◽  
Degradation Rate ◽  
Carbon Chain ◽  
Hydrocarbon Contamination ◽  
Short Chain ◽  
Petroleum Contamination ◽  
Rate Curve ◽  
Petroleum Hydrocarbon Contamination

Petroleum contamination has become one of the major soil contaminations. Aiming at petroleum hydrocarbon contamination, the multi-group opposite experiments is set; this paper use some petroleum hydrocarbon-decomposing bacteria to remedy the soil contaminated by different carbon chain petroleum hydrocarbons. Compare and study the remedial results, and study the growth of the bacteria in the decomposing process. The Study shows that the degradation rate of the bacteria to short-chain petroleum hydrocarbons is relatively high; Within 40 days without nutrient substance, degradation rate of bacteria to gasoline and diesel is 80%, degradation rate of bacteria to aromatics and lubricants is 50%, the trend of bacteria’s growth curve and the degradation rate curve of each component are approximate.

scholarly journals Phytoremediation of hydrocarbon-contaminated soils with emphasis on the effect of petroleum hydrocarbons on the growth of plant species

2009 ◽  
Vol 89 (1) ◽  
pp. 21-29 ◽  
Author(s):  
Ravanbakhsh Shirdam ◽  
Ali Daryabeigi Zand ◽  
Gholamreza Nabi Bidhendi ◽  
Nasser Mehrdadi
Keyword(s):  
Plant Species ◽  
Contaminated Soil ◽  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Petroleum Hydrocarbon ◽  
Green Technology ◽  
Hydrocarbon Contamination ◽  
Total Petroleum Hydrocarbons ◽  
Control Treatment ◽  
Polluted Soils

To date, many developing countries such as Iran have almost completely abandoned the idea of decontaminating oil-polluted soils due to the high costs of conventional (physical/chemical) soil remediation methods. Phytoremediation is an emerging green technology that can become a promising solution to the problem of decontaminating hydrocarbon-polluted soils. Screening the capacity of native tolerant plant species to grow on aged, petroleum hydrocarbon-contaminated soils is a key factor for successful phytoremediation. This study investigated the effect of hydrocarbon pollution with an initial concentration of 40 000 ppm on growth characteristics of sorghum (Sorghum bicolor) and common flax (Linum usitatissumum). At the end of the experiment, soil samples in which plant species had grown well were analyzed for total petroleum hydrocarbons (TPHs) removal by GC-FID. Common flax was used for the first time in the history of phytoremediation of oil-contaminated soil. Both species showed promising remediation efficiency in highly contaminated soil; however, petroleum hydrocarbon contamination reduced the growth of the surveyed plants significantly. Sorghum and common flax reduced TPHs concentration by 9500 and 18500 mg kg‑1, respectively, compared with the control treatment.

scholarly journals Surfactant Enhanced Bioremediation of Hydrocarbon Contaminated Soils Using Alkyl Polyglucoside

2021 ◽  
pp. 17-28
Author(s):  
C. E. Ezekiel ◽  
Leo C. Osuji ◽  
M. C. Onojake
Keyword(s):  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Soil Samples ◽  
Hydrocarbon Contamination ◽  
Total Petroleum Hydrocarbons ◽  
Alkyl Polyglycosides ◽  
Enhanced Biodegradation ◽  
Enhanced Bioremediation ◽  
Reduction Percentage ◽  
Alkyl Polyglucoside

Bioremediation is an efficient and environmentally friendly method for the degradation of petroleum hydrocarbons in contaminated soils. This study investigated the effects of biosurfactant alkyl polyglycosides (APG) on enhanced biodegradation of petroleum hydrocarbon contaminated soils.  Three soil samples were contaminated with two different grades of crude oil (medium and Light). Alkyl polyglucoside was synthesised and subjected to FTIR for comfirmation of the product before it was applied in the remediation of contaminated soil. The alkyl polyglucoside is used as a treatment regime in the remediation of the hydrocarbon contamination in the three soil samples. Results of total petroleum hydrocarbons (TPH) before remediation with bio-surfactant showed that samples contaminated with medium crude for Eneka, Ozuoba and Rukpokwu were 15744.00 mg/kg, 11359.00 mg/kg and 11470.00 mg/kg respectively and after remediation reduced to 4276.00 mg/kg, 4265.00 mg/kg, and 3205.00 mg/kg, showing a reduction percentage of 72.84%, 62.44% and 72.05% respectively. Soil samples contaminated with light crude showed result of TPH of 11339.00 mg/kg, 10662 mg/kg and 10226 mg/kg and after remediation reduced to 2981 mg/kg, 3879 mg/kg, and 4245 mg/kg respectively showing a reduction percentage of 73.71%, 63.62 % and 58.49% respectively. The enhanced efficiency of the bio-surfactant at degrading total petroleum hydrocarbons was achieved as a result of the increased solubility thus improving the bioavailability of the hydrocarbons due to the action of the alkyl polyglucoside.

scholarly journals Comparative Study on Different Remediation Strategies Applied in Petroleum-Contaminated Soils

2020 ◽  
Vol 17 (5) ◽  
pp. 1606 ◽  
Author(s):  
Jia-Qi Cui ◽  
Qing-Sheng He ◽  
Ming-Hui Liu ◽  
Hong Chen ◽  
Ming-Bo Sun ◽  
...  
Keyword(s):  
Removal Efficiency ◽  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Soil Moisture Content ◽  
Alpha Diversity ◽  
Physicochemical Characteristics ◽  
Community Response ◽  
Environmental Condition ◽  
Promoting Effect ◽  
Remediation Strategies

Due to the increasing pollution by petroleum hydrocarbons (PHs), it is an important task to develop eco-friendly and highly efficient methods for remediating petroleum-contaminated soils. In this study, bioremediation technology was applied to remediate PHs contaminated soils, and the bacterial community structure and physicochemical characteristics of the soil treated using different bioremediation regimens were analyzed. Compared with the control condition (S0), the PHs removal efficiency of biostimulation (S2) and bioaugmentation (S3) was increased significantly. Combined biostimulation with bioaugmentation (S4) had the highest PHs removal efficiency, up to 60.14 ± 4.12%. Among all the selected remediation strategies (S1–S4, S1: soil moisture content: 25–30%), the bacterial alpha-diversity was higher than in S0. The genera Acinetobacter, Escherichia-Shigella, Bacteroides, Microbacterium, and Parabacteroides were found to greatly contribute to PHs’ degradation. In the group S4, the PH-degraders and soil enzyme activity were higher than in the other remediation regimens, and these indices gradually decreased in the mid-to-later periods of all remediation tests. Additionally, the abundance of alkB and nah genes was increased by improving the environmental condition of the microorganism communities. Redundancy analysis (RDA) revealed that the total nitrogen (TN) and total phosphorus (TP) had a positive correlation with total PHs degradation. This study offers insights into the microbial community response to environmental factors during bioremediation, which shows a promoting effect in enhancing the efficiency of PHs remediation.

scholarly journals Influence of Physicochemical Properties and Parent Material on Chromium Fractionation in Soils

Processes ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 1073
Author(s):  
Claudia Campillo-Cora ◽  
Laura Rodríguez-González ◽  
Manuel Arias-Estévez ◽  
David Fernández-Calviño ◽  
Diego Soto-Gómez
Keyword(s):  
Physicochemical Properties ◽  
Contaminated Soils ◽  
Research Work ◽  
Parent Material ◽  
The Other ◽  
Oxidation States ◽  
Diethylenetriaminepentaacetic Acid ◽  
Remediation Strategies ◽  
Exchangeable Aluminum ◽  
Efficient Extraction

Chromium is an element that possess several oxidation states and can easily pass from one to another, so its behavior in soils is very complex. For this reason, determining its fate in the environment can be difficult. In this research work we tried to determine which factors affect the chromium fractionation in natural soils, conditioning chromium mobility. We paid special attention to the parent material. For this purpose, extraction experiments were carried out on spiked soils incubated for 50–60 days, using H2O, CaCl2 and diethylenetriaminepentaacetic acid (DTPA). The most efficient extraction rate in all soils was achieved using water, followed by CaCl2 and DTPA. We obtained models with an adjusted R2 of 0.8097, 0.8471 and 0.7509 for the H2O Cr, CaCl2 Cr and DTPA Cr respectively. All models were influenced by the amount of chromium added and the parent material: amphibolite and granite influenced the amount of H2O Cr extracted, and schist affected the other two fractions (CaCl2 and DTPA). Soil texture also played an important role in the chromium extraction, as well as the amounts of exchangeable aluminum and magnesium, and the bioavailable phosphorus. We concluded that it is possible to make relatively accurate predictions of the behavior of the different Cr fractions studied, so that optimized remediation strategies for chromium-contaminated soils can be designed on the basis of a physicochemical soil characterization.

scholarly journals Phytoremediation Potential of Native Herbaceous Plant Species Growing on a Paradigmatic Brownfield Site

2021 ◽  
Vol 232 (7) ◽  
Author(s):  
N. Matanzas ◽  
E. Afif ◽  
T. E. Díaz ◽  
J. R. Gallego
Keyword(s):  
Plant Species ◽  
Contaminated Soils ◽  
Native Species ◽  
Low Cost ◽  
Plantago Lanceolata ◽  
Herbaceous Plant ◽  
Plant Interactions ◽  
Aerial Parts ◽  
Medicago Lupulina ◽  
Icp Ms

AbstractPhytomanagement techniques using native species allow the recovery of contaminated soils at low cost and circumvent the ecological risks associated with the use of non-native species. In this context, a paradigmatic brownfield megasite highly contaminated by As and Pb was sampled in order to analyze soil–plant interactions and identify plant species with phytoremediation potential. A survey was first carried out in a 20-ha area to obtain an inventory of species growing spontaneously throughout the site. We then performed another survey in the most polluted sub-area (1 ha) within the site. Pseudototal concentrations of contaminants in the soil, aerial parts of the plants, and roots were measured by ICP-MS. A detailed habitat classification was done, and a specific index of coverage was applied by means of a 1-year quadrat study in various sampling stations. Results converged in the selection of six herbaceous species (Dysphania botrys, Lotus corniculatus, Lotus hispidus, Plantago lanceolata, Trifolium repens, Medicago lupulina). All of these plants are fast-growing, thereby making them suitable for use in phytostabilization strategies. Furthermore, they are all easy to grow and propagate and are generally self-sustaining. All six plants showed accumulation factors below 1, thus revealing them as pseudomethallophytes and excluders. However, L. hispidus and M. lupulina showed translocation capacity and are considered worthy of further study.

scholarly journals Characterization of microbial response to petroleum hydrocarbon contamination in a lacustrine ecosystem

2021 ◽  
Author(s):  
Emilio D’Ugo ◽  
Milena Bruno ◽  
Arghya Mukherjee ◽  
Dhrubajyoti Chattopadhyay ◽  
Roberto Giuseppetti ◽  
...  
Keyword(s):  
Oil Spill ◽  
Petroleum Hydrocarbon ◽  
Oil Spills ◽  
Oil Pollution ◽  
Primary Source ◽  
Hydrocarbon Contamination ◽  
Petroleum Hydrocarbon Contamination ◽  
Microbial Response ◽  
Pollution Event ◽  
Hydrocarbonoclastic Potential

AbstractMicrobiomes of freshwater basins intended for human use remain poorly studied, with very little known about the microbial response to in situ oil spills. Lake Pertusillo is an artificial freshwater reservoir in Basilicata, Italy, and serves as the primary source of drinking water for more than one and a half million people in the region. Notably, it is located in close proximity to one of the largest oil extraction plants in Europe. The lake suffered a major oil spill in 2017, where approximately 400 tons of crude oil spilled into the lake; importantly, the pollution event provided a rare opportunity to study how the lacustrine microbiome responds to petroleum hydrocarbon contamination. Water samples were collected from Lake Pertusillo 10 months prior to and 3 months after the accident. The presence of hydrocarbons was verified and the taxonomic and functional aspects of the lake microbiome were assessed. The analysis revealed specialized successional patterns of lake microbial communities that were potentially capable of degrading complex, recalcitrant hydrocarbons, including aromatic, chloroaromatic, nitroaromatic, and sulfur containing aromatic hydrocarbons. Our findings indicated that changes in the freshwater microbial community were associated with the oil pollution event, where microbial patterns identified in the lacustrine microbiome 3 months after the oil spill were representative of its hydrocarbonoclastic potential and may serve as effective proxies for lacustrine oil pollution.

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