scholarly journals Determination of Deoxynivalenol Biomarkers in Italian Urine Samples

Toxins ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 441 ◽  
Author(s):  
De Santis ◽  
Debegnach ◽  
Miano ◽  
Moretti ◽  
Sonego ◽  
...  

Deoxynivalenol (DON) is a mycotoxin mainly produced by Fusarium graminearum that can contaminate cereals and cereal-based foodstuff. Urinary DON levels can be used as biomarker for exposure assessment purposes. This study assessed urinary DON concentrations in Italian volunteers recruited by age group, namely children, adolescents, adults, and the elderly. In addition, vulnerable groups, namely vegetarians and pregnant women, were included in the study. To determine the urinary DON, its glucuronide and de-epoxydated (DOM-1) forms, an indirect analytical approach was used, measuring free DON and total DON (as sum of free and glucuronides forms), before and after enzymatic treatment, respectively. Morning urine samples were collected on two consecutive days, from six different population groups, namely children, adolescent, adults, elderly, vegetarians and pregnant women. Total DON was measured in the 76% of the collected samples with the maximum incidences in children and adolescent age group. Urine samples from children and adolescent also showed the highest total DON levels, up to 17.0 ng/mgcreat. Pregnant women had the lowest positive samples per category (40% for day 1 and 43% for day 2, respectively), low mean levels of total DON (down to 2.84 ng/mgcreat) and median equal to 0 ng/mgcreat. Estimation of DON dietary intake reveals that 7.5% of the total population exceeds the TDI of 1 μg/kg bw/day set for DON, with children showing 40% of individuals surpassing this value (male, day 2).

Author(s):  
Semra Akkoz Cevik ◽  
Emine Karacan ◽  
Aysegul Kılıçlı

Background: Superstition and traditional practices regarding pregnancy exist in various cultures around the world and are passed down from generation to generation. As a result of the literature review, it was observed that there weren't prospective and cross-sectional studies involving traditional practices and dietary preferences of pregnant women before and after determining the gender according to the gender of the baby they want. The purpose of this study was to determine dietary preferences, superstitions and traditional practices before and after determining the gender of the baby according to the desired gender of the baby among pregnant women.Methods: The research was a descriptive cross-sectional study and adopted the pretest-posttest research design. As the data collection tool, both investigators-designed questionnaire forms and the non-functional beliefs and practices scale (NFBPS) related to pregnancy to the same pregnant women were applied before and after the determination of baby gender in the first and second trimesters respectively.Results: The study found that 85% of the pregnant women thought that dietary preferences during pregnancy have an impact on the gender of the true baby. The results showed that superstitions and traditional practices were known and practiced by pregnant women at a young age, uneducated, living in the province and having a large family type (p<0.05).Conclusions: Health professionals, should pay attention to less educated, younger pregnant women to reinforce positive cultural health practices, discourage to them from using harmful ones superstition and traditional practice by providing non-critical scientific explanations. 


1972 ◽  
Vol 52 (1) ◽  
pp. 119-128 ◽  
Author(s):  
JORMA HEIKKILÄ

SUMMARY The amounts of oestriol and 15α-hydroxyoestriol (oestetrol) were determined in 24-h urine samples collected from pregnant women before and after delivery. In addition, the concentrations of these oestrogens were estimated in the urine of the newborn infants born to these mothers. The excretion of both these oestrogens decreased exponentially in maternal urine after delivery, the rate of decrease being significantly slower for oestetrol. The concentration of oestriol and oestetrol in the urine of the newborn infants also fell rapidly after delivery, the reduction being a linear function of time. The concentration of oestetrol in the urine of the newborn infant was relatively high in comparison to that of oestriol, and during the first day of life was the same as in maternal urine before delivery.


1978 ◽  
Vol 24 (11) ◽  
pp. 1958-1961 ◽  
Author(s):  
J McCready ◽  
G D Braunstein ◽  
D Helm ◽  
M E Wade

Abstract The choriogonadotropin beta-subunit radioimminoassay has been used extensively to measure human choriogonadotropin in the sera of pregnant women and individuals with trophoblastic and nontrophoblastic tumors. Unmodified, this method cannot be used to measure choriogonadotropin in urine because of interfering substances. We circumvented the non-parallelism between the standards and serial dilutions of urine containing choriogonadotropin by adding pooled urine from men to the standard tubes and limiting the volume of urine to 100 microliter. This modified assay has a sensitivity of 3 int. units/liter of urine and is specific for choriogonadotropin concentrations of 40 int. units/liter of urine. Analytical recovery of choriogonadotropin added to urine ranged from 96 to 105%. The within-assay CV was 7.6%; the between-assay CV was 11.8%. Concentrations of choriogonadotropin in concurrently collected serum and urine samples from pregnant women correlated well. The test can be performed within 24 h by using the double-antibody method for separating bound from free hormone, or in 3 h with a dioxane method. The assay is about 20-fold more sensitive than the 2-min or 2-h slide and tube pregnancy tests, and seven-to 12-fold more sensitive than the radioreceptor assay.


Author(s):  
Onuigwe Festus Uchechukwu ◽  
Khadija Bello Tudu ◽  
Erhabor Osaro ◽  
Buhari Hauwa Ali ◽  
Bagudo Aliyu Ibrahim ◽  
...  

Background and Aim: Obstetric complications are part of the leading causes of maternal mortality worldwide. This study was carried out to investigate the effect of   complications on the Prothrombin time (PT), activated partial thromboplastin time (APTT), and platelet count (PLC). Women with obstetric complications were recruited as subjects). Pregnant women without complications were included as controls. Study Design: This is a case-control study. Place and Duration of Study: The study took place in Obstetrics and Gynaecology Department of Specialist Hospital Sokoto and the duration was six months. Methodology: Pregnant women aged 18–41 years (mean age 29.5 years) were recruited for the study. The determination of PT and APTT was done by manual methods using commercially prepared Agappe reagent kits, whereas PLC was done by manual methods using a haemocytometer (Improved Neubauer counting chamber). Data were analyzed using SPSS version 23. Results: The results of PT and APTT were significantly higher among women with obstetric complications (14.26±0.23 seconds and 31.32±0.70 second) compared to normal pregnant women (13.00 ± 0.13 seconds and 29.66±0.33) (P<0.05). The PLC was significantly lower among women with obstetric complications compared to women without obstetric complications (P< 0.05).Obstetric complications in subject show a significant association when compared with PT and APTT (P< 0.05). The age group of subjects shows a significant association when compared with APTT (P< 0.05). Conclusion: Findings from this study have shown that obstetric complications causes decrease in platelet count and prolongation in PT and APTT. There is need to assess the PT, APTT and Platelet count routinely for pregnant women to improve the ante-natal care in Specialist Hospital Sokoto.


2021 ◽  
Vol 12 (2) ◽  
pp. 130-140
Author(s):  
Olesia Datsko ◽  
◽  
Nataliya Nakonechna ◽  
Olha Patsula ◽  
◽  
...  

The article outlines systemic threats, substantiates the need and highlights mechanisms for strengthening the economic security of the citizens of Ukraine in the context of exacerbation of socio-political crisis and external challenges. The existing in inconsistency of basic social standards in Ukraine and their institutional provision with real conditions and needs of citizens causes inadequate state guarantee of its beneficiaries (citizens) rights to life, earnings, housing, health care and, in general, - to self-reproduction and development. Given the aggravation of socio-political and socio-economic crises, deepened by hostilities in eastern Ukraine, the annexation of the Autonomous Republic of Crimea, ensuring economic security of citizens, especially vulnerable groups - migrants, large and young families, the elderly is highly important. The main reason for the high level of vulnerability of socio-economic rights of citizens is the imperfection of state and regional policy, inefficient management of state resources, which leads to their privatization or hidden unfair redistribution of shadow schemes in favor of political elites. As a result, this fact has led to excessive stratification of the population in terms of income, when about 80% of the total population of Ukraine receives a monthly income below the real subsistence level, the spread of poverty among workers. The analysis shows that the reforms in Ukraine are carried out without proper forecasting and assessment of the expected effectiveness, taking into account the key performance indicators (KRI) to ensure the economic security of the main beneficiary of the state - a citizen of Ukraine. Further reform of the socio-economic sphere requires the introduction of institutional changes with the obligatory priority of economic security of the citizens.


2017 ◽  
Vol 11 (04) ◽  
pp. 469-474 ◽  
Author(s):  
Veerale Panchal ◽  
Deepa Gurunathan ◽  
A. k. Shanmugaavel

ABSTRACT Objective: To assess the dietary and oral hygiene pattern before and after the usage of cariometer app. Materials and Methods: Dietary score recorded by cariometer was assessed for 7 days for preschool children in the age group of 2–6 years. The frequency of brushing and mouth rinsing was assessed before and after the usage of app. Paired t-test was conducted to compare the dietary score, brushing frequency, and rinsing frequency pre and post the use of the app. Results: There was a significant improvement in the dietary pattern followed by the patients at the day 7 as compared to day 1. About 90% of children brushed twice at day 7 of the use of cariometer. There was a significant increase in the frequency of rinsing after meals at day 7 as compared to day 1 of the use of cariometer. Conclusion: There was a significant improvement in the dietary pattern and the oral hygiene habit after the use of app.


2008 ◽  
Vol 11 (4) ◽  
pp. 405-412 ◽  
Author(s):  
Fernando Vio ◽  
Cecilia Albala ◽  
Juliana Kain

AbstractObjectiveTo analyse the nutritional status component of the nutrition transition in Chile in relation to the evolution of obesity rates in all age groups until 2005, comparing these with the goals established by the Ministry of Health for the period 2000–2010.Design and settingA descriptive study which includes data on the nutritional status of the Chilean population categorised by age. The data originate from: (1) institutions which administer nutritional programmes – the National Board for Day-Care Centres (JUNJI) and the National Board for School Assistance and Scholarships (JUNAEB); (2) the Ministry of Health and the National Institute of Statistics; and (3) epidemiological studies which include adolescents and the elderly.ResultsThe prevalence of obesity in pre-school children attending JUNJI was 10.6% in 2005; by age group, it was 6% in 2-year-olds, 11% in 3-year-olds and 14% in 4-year-olds. Among schoolchildren in first grade, obesity prevalence was 18.5%. In pregnant women, obesity has increased from 12% in 1987 to 33% in 2004. For adults, the 2003 National Health Survey showed that the prevalence of obesity (body mass index (BMI) ≥ 30 kg m−2) was 22% and of morbid obesity (BMI≥40 kg m−2), 1.3%. Obesity varied according to gender and educational level, being higher among women (25% vs. 19% in men) and adults from low socio-economic levels. In the elderly there was a high prevalence of obesity in the 60–64 years age group for both men (35.6%) and women (44.1%), decreasing to 18% and 26%, respectively, in those aged 75 years and older.ConclusionsThe goals for the decade (2000–2010) consider a reduction of obesity rates from 10% to 7% in pre-school children attending JUNJI, and from 16% to 12% in schoolchildren attending first grade. For pregnant women, the goal is to reduce the prevalence from 32% to 28%. Despite the implementation of initiatives in nutrition and physical activity, these have been insufficient to shift the rising trend in obesity. The explanation could be that after a rapid rise in obesity in children and pregnant women between 1987 and 2000, a stabilisation period or a ‘plateau’ is observed. In that situation, very effective interventions are required to reduce obesity, because it is extremely difficult to reverse the trend. Some positive experiences are being implemented in Chile, but government priorities are not focused in health promotion. A comprehensive State Policy in health promotion, that includes the public and private sectors related with obesity, is needed to reverse this trend.


2007 ◽  
Vol 53 (9) ◽  
pp. 1623-1628 ◽  
Author(s):  
Alfredo Leaños-Miranda ◽  
Janeth Márquez-Acosta ◽  
Fernando Romero-Arauz ◽  
Guadalupe M Cárdenas-Mondragón ◽  
Roxana Rivera-Leaños ◽  
...  

Abstract Background: The protein:creatinine ratio in random, untimed urine samples correlates with 24-h protein excretion in pregnant women with and without hypertension. Nevertheless, whether this ratio is appropriate as a screening test for proteinuria is still unclear, in part because of the paucity of large studies. Methods: We measured protein:creatinine ratios in random urine samples and protein contents of 24-h urine samples in a cross-sectional study of 927 hospitalized pregnant women at ≥20-weeks of gestational age and in a 2nd cohort of 161 pregnant women. In the 2nd group, urine specimens were obtained before and after completion of the 24-h collections, avoiding 1st-morning void specimens. Results: Protein excretion was ≥300 mg/24 h in 282 patients (30.4%). The urine protein:creatinine ratio and the 24-h protein excretion were significantly correlated (r = 0.98, P &lt;0.001). The protein:creatinine ratio as an indicator of protein excretion ≥300 mg/24 h was ≥0.3. The sensitivity and specificity were 98.2% and 98.8%, respectively. Positive and negative predictive values were 97.2% and 99.2%, respectively, and positive and negative likelihood ratios were 79.2 and 0.02, respectively. The diagnostic accuracy of the urinary protein:creatinine ratio was corroborated in the 2nd cohort of patients, which also showed no statistically significant difference in protein:creatinine ratio between samples obtained &gt;24 h apart. Conclusions: Random urinary protein:creatinine ratio is a reliable indicator of significant proteinuria (&gt;300 mg/day) in nonambulatory pregnant women, irrespective of sampling time during the daytime. The protein:creatinine ratio may be reasonably used as an alternative to the 24-h urine collection method.


2021 ◽  
Vol 3 (2) ◽  
pp. 165
Author(s):  
Safitri Safitri

Covid-19 is a disease caused by a new type of coronavirus, namely Sars-CoV-2, which was first reported in Wuhan China on December 31, 2019. Pregnant women are one of the vulnerable groups at risk of being infected with Covid-19 due to physiological changes that have resulted in a decrease partial immunity. Initial studies found that many pregnant women do not implement health protocols in their daily lives and lack knowledge of Covid-19 and than prevention. One of the efforts that can be made in providing knowledge related to prevention measures for Covid-19 transmission is through online educational videos. This community service activity was implemented on pregnant women in Jambi City, with a total sample of 20 pregnant women. The expected outcome target is to increase knowledge between before and after being given an online educational video. The method used is online health education using educational video media that can be accessed via YouTube. Knowledge of pregnant women was assessed through the results of questionnaire via google form. Monitoring and evaluation of activities using the One Group Pretest and Post-test Design approaches. The result of service has increased knowledge after being given online educational videos as an effort to prevent Covid-19 transmission


2016 ◽  
Vol 1 (2) ◽  
Author(s):  
RD Rahayu

Abstract: MMN, Weight. Pregnant women are one of the vulnerable groups may suffer from anemia because of events that occurred hemodelusi sat pregnancy. MMN is a supplement that contains multivitamins, iron and folate given to pregnant women as one of the Indonesian health ministry programs in an effort to prevent anemia during pregnancy. MMN order to determine the effect on weight gain for pregnant women. The research method to experiment with randomized. The population of 24 male mice. Twenty rats were given MMN. Data analysis using independent t-test. Results: Based on the average value MMN can increase weight gain. There is a difference in weight gain between before and after given MMN.


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