scholarly journals Global Metabolic Profiling of Baculovirus Infection in Silkworm Hemolymph Shows the Importance of Amino-Acid Metabolism

Viruses ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 841
Author(s):  
Min Feng ◽  
Shigang Fei ◽  
Junming Xia ◽  
Mengmeng Zhang ◽  
Hongyun Wu ◽  
...  
Keyword(s):  
Amino Acid ◽  
Bombyx Mori ◽  
Metabolic Profiling ◽  
Amino Acid Metabolism ◽  
Time Course ◽  
Acid Metabolism ◽  
Cell Metabolism ◽  
Systemic Infection ◽  
Silkworm Hemolymph ◽  
Baculovirus Infection

Viruses rely on host cell metabolism to provide the necessary energy and biosynthetic precursors for successful viral replication. Infection of the silkworm, Bombyx mori, by Bombyx mori nucleopolyhedrovirus (BmNPV), has been studied extensively in the past to unravel interactions between baculoviruses and their lepidopteran hosts. To understand the interaction between the host metabolic responses and BmNPV infection, we analyzed global metabolic changes associated with BmNPV infection in silkworm hemolymph. Our metabolic profiling data suggests that amino acid metabolism is strikingly altered during a time course of BmNPV infection. Amino acid consumption is increased during BmNPV infection at 24 h post infection (hpi), but their abundance recovered at 72 hpi. Central carbon metabolism, on the other hand, particularly glycolysis and glutaminolysis, did not show obvious changes during BmNPV infection. Pharmacologically inhibiting the glycolytic pathway and glutaminolysis also failed to reduce BmNPV replication, revealing that glycolysis and glutaminolysis are not essential during BmNPV infection. This study reveals a unique amino acid utilization process that is implemented during BmNPV infection. Our metabolomic analysis of BmNPV-infected silkworm provides insights as to how baculoviruses induce alterations in host metabolism during systemic infection.

scholarly journals Metabolic profiling reveals disorder of amino acid metabolism in four brain regions from a rat model of chronic unpredictable mild stress

FEBS Letters ◽  
2008 ◽  
Vol 582 (17) ◽  
pp. 2627-2636 ◽  
Author(s):  
Yan Ni ◽  
Mingming Su ◽  
Jinchao Lin ◽  
Xiaoyan Wang ◽  
Yunping Qiu ◽  
...  
Keyword(s):  
Amino Acid ◽  
Rat Model ◽  
Metabolic Profiling ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Brain Regions ◽  
Mild Stress ◽  
Chronic Unpredictable Mild Stress

scholarly journals Extracellular Matrix Stiffness: New Areas Affecting Cell Metabolism

2021 ◽  
Vol 11 ◽  
Author(s):  
Heming Ge ◽  
Mengxiang Tian ◽  
Qian Pei ◽  
Fengbo Tan ◽  
Haiping Pei
Keyword(s):  
Extracellular Matrix ◽  
Amino Acid ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Cell Metabolism ◽  
Metabolic Reprogramming ◽  
Matrix Stiffness ◽  
Extracellular Matrix Stiffness ◽  
The Relationship ◽  
Novel Therapeutic

In recent years, in-depth studies have shown that extracellular matrix stiffness plays an important role in cell growth, proliferation, migration, immunity, malignant transformation, and apoptosis. Most of these processes entail metabolic reprogramming of cells. However, the exact mechanism through which extracellular matrix stiffness leads to metabolic reprogramming remains unclear. Insights regarding the relationship between extracellular matrix stiffness and metabolism could help unravel novel therapeutic targets and guide development of clinical approaches against a myriad of diseases. This review provides an overview of different pathways of extracellular matrix stiffness involved in regulating glucose, lipid and amino acid metabolism.

Disturbed energy and amino acid metabolism with their diagnostic potential in mitral valve disease revealed by untargeted plasma metabolic profiling

Metabolomics ◽  
2019 ◽  
Vol 15 (4) ◽  
Author(s):  
Limiao Jiang ◽  
Jing Wang ◽  
Rui Li ◽  
Ze-min Fang ◽  
Xue-Hai Zhu ◽  
...  
Keyword(s):  
Amino Acid ◽  
Mitral Valve ◽  
Mitral Valve Disease ◽  
Metabolic Profiling ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Valve Disease ◽  
Diagnostic Potential

Firsthand and secondhand exposure levels of maltol-flavored electronic nicotine delivery system (ENDS) vapors disrupt amino acid metabolism

2021 ◽  
Author(s):  
Zachery R Jarrell ◽  
Matthew Ryan Smith ◽  
Xiaojia He ◽  
Michael Orr ◽  
Dean P Jones ◽  
...  
Keyword(s):  
Amino Acid ◽  
Delivery System ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Cell Metabolism ◽  
Airway Epithelial ◽  
Safe Alternative ◽  
Lung Metabolism ◽  
Nicotine Delivery ◽  
Exposure Levels

Abstract Electronic nicotine delivery system (ENDS) use has become a popular, generally regarded as safe, alternative to tobacco use. The e-liquids used for ENDS vapor generation commonly contain flavoring agents, such as maltol, which have been subjected to little investigation of their effects on lung health from ENDS usage. In the present study, we examined the impacts of firsthand (3.9 mM) and secondhand (3.9 µM) exposure levels to maltol-flavored ENDS vapors on lung metabolism. Bronchial airway epithelial BEAS-2B cells were exposed to ENDS vapors using a robotic system for controlled generation and delivery of exposures, and the effects on metabolism were evaluated using high-resolution metabolomics. The results show that maltol in e-liquids impacts lung airway epithelial cell metabolism at both firsthand and secondhand exposure levels. The effects of maltol were most notably seen in amino acid metabolism; oxidative stress was present with ENDS and not affected by maltol. Many effects of firsthand exposure were also observed with secondhand exposure, suggesting need for systematic investigation of both firsthand and secondhand effects of flavored ENDS vapors on lung metabolism and risk of lung disease.

scholarly journals 1H NMR-Based Metabolic Profiling Reveals the Effects of Fluoxetine on Lipid and Amino Acid Metabolism in Astrocytes

2015 ◽  
Vol 16 (12) ◽  
pp. 8490-8504 ◽  
Author(s):  
Shunjie Bai ◽  
Chanjuan Zhou ◽  
Pengfei Cheng ◽  
Yuying Fu ◽  
Liang Fang ◽  
...  
Keyword(s):  
Amino Acid ◽  
1H Nmr ◽  
Metabolic Profiling ◽  
Amino Acid Metabolism ◽  
Acid Metabolism

scholarly journals Amino Acid Metabolism in Lupus

2021 ◽  
Vol 12 ◽  
Author(s):  
Michihito Kono ◽  
Nobuya Yoshida ◽  
George C. Tsokos
Keyword(s):  
T Cells ◽  
Amino Acid ◽  
T Cell ◽  
T Cell Activation ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Cell Metabolism ◽  
Cell Subset ◽  
Metabolic Reprogramming ◽  
Acid Oxidation

T cell metabolism is central to cell proliferation, survival, differentiation, and aberrations have been linked to the pathophysiology of systemic autoimmune diseases. Besides glycolysis and fatty acid oxidation/synthesis, amino acid metabolism is also crucial in T cell metabolism. It appears that each T cell subset favors a unique metabolic process and that metabolic reprogramming changes cell fate. Here, we review the mechanisms whereby amino acid transport and metabolism affects T cell activation, differentiation and function in T cells in the prototype systemic autoimmune disease systemic lupus erythematosus. New insights in amino acid handling by T cells should guide approaches to correct T cell abnormalities and disease pathology.

scholarly journals Elucidation of salt stress defense and tolerance mechanisms of marine red yeast Sporobolomyces pararoseus NGR using transcriptomic and metabolomic profiling

2019 ◽  
Author(s):  
Chunji Li ◽  
Die Zhao ◽  
Ning Zhang ◽  
Bingxue Li
Keyword(s):  
Salt Stress ◽  
Lipid Metabolism ◽  
Amino Acid ◽  
Network Analysis ◽  
Transcriptome Analysis ◽  
Metabolic Profiling ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Salt Tolerant ◽  
Tolerance Mechanisms

Background. Salinity stress is one of the most environmental stresses in agricultural regions worldwide. Salinity inhibits shoot and root growth of various crops, which culminate in reductions in the quality and yield. It is of crucial to understand the molecular biological mechanisms of salt stress responses and defenses in order to enhance crops salt-tolerance. Sporobolomyces pararoseus is a member of marine red yeasts. Since marine red yeast has been naturally selected for its long-term survival in high-salt marine ecosystems, some unique salt-tolerant mechanism has been developed. Little research has conducted so far by considering S. pararoseus as model microorganisms to study salt stress tolerance mechanisms. A better understanding of the mechanisms mediating salt stress of S. pararoseus NGR will provide valuable information for enhancing the crops salt-tolerant via genetic engineering. Methods. S. pararoseus NGR (CGMCC 2.5280) cultures were treated with initial NaCl concentrations of 0.75 M throughout 3 days of growth period. Transcriptome analysis was performed using RNA-seq to study the differentially expressed genes (DEGs) between the NaCl-treated cells and the control cells. Metabolome analysis was performed using the LC-MS/MS untargeted metabolic profiling to study the differentially accumulated metabolites between the NaCl-treated cells and the control cells. Co-expression network analysis was carried out using the screening parameters of correlation coefficient = 0.99 and p-value = 0.01. Transcriptome analysis results were confirmed by real-time quantitative PCR (RT-qPCR). Results. After sequencing, de novo assembly and quantitative assessment, 9,533 unigenes were finally generated with an average length of 1,538 bp. A total of 3,849 DGEs were identified in NaCl-treated cultures, including 2,019 up-regulated genes and 1,830 down-regulated genes. Screening of metabolite features with untargeted metabolic profiling of all samples in NaCl-treated and control group, we characterized 4,862 compounds from the LC–MS/MS-based dataset. An integrated analysis of transcriptome and metabolome indicated that amino acid metabolism, carbohydrate metabolism, and lipid metabolism is significantly enriched in response to salt stress. Co-expression network analysis showed that 28 genes and 8 metabolites played an important role in the response of S. pararoseus NGR and defense against salt stress, which provides valuable clues for subsequent validation. Together, our results suggested that the most primary salt-tolerant mechanism of the S. pararoseus NGR is the biosynthesis of carotenoids, and torulene showed the dominated effect among them. Moreover, amino acid metabolism, carbohydrate metabolism and lipid metabolism act as its secondary salt-tolerant mechanism.

scholarly journals GC–MS metabolic profiling reveals fructose-2,6-bisphosphate regulates branched chain amino acid metabolism in the heart during fasting

Metabolomics ◽  
2019 ◽  
Vol 15 (2) ◽  
Author(s):  
Albert Batushansky ◽  
Satoshi Matsuzaki ◽  
Maria F. Newhardt ◽  
Melinda S. West ◽  
Timothy M. Griffin ◽  
...  
Keyword(s):  
Amino Acid ◽  
Metabolic Profiling ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Branched Chain Amino Acid ◽  
Branched Chain

scholarly journals Metabolic Profiling of Serum Reveals Significant Changes in Lipid and Amino Acid Metabolism in A Lipodystrophy Mouse Model

2018 ◽  
Vol 32 (S1) ◽  
Author(s):  
Yawen Zhou ◽  
Kenneth KY Cheng ◽  
Aimin Xu ◽  
Ruby LC Hoo
Keyword(s):  
Amino Acid ◽  
Mouse Model ◽  
Metabolic Profiling ◽  
Amino Acid Metabolism ◽  
Acid Metabolism
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