scholarly journals Influence of Herbicide Exposure and Ranavirus Infection on Growth and Survival of Juvenile Red-Eared Slider Turtles (Trachemys scripta elegans)

Viruses ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1440
Author(s):  
Rachel M. Goodman ◽  
Edward Davis Carter ◽  
Debra L. Miller
Keyword(s):  
Infection Rate ◽  
Culture Medium ◽  
Control Cell ◽  
Environmental Stressors ◽  
Trachemys Scripta ◽  
Control Group ◽  
Direct Effects ◽  
Trachemys Scripta Elegans ◽  
Growth And Survival ◽  
Infected Cell Lysate

Ranaviruses are an important wildlife pathogen of fish, amphibians, and reptiles. Previous studies have shown that susceptibility and severity of infection can vary with age, host species, virus strain, temperature, population density, and presence of environmental stressors. Experiments are limited with respect to interactions between this pathogen and environmental stressors in reptiles. In this study, we exposed hatchling red-eared slider turtles (Trachemys scripta elegans) to herbicide and ranavirus treatments to examine direct effects and interactions on growth, morbidity, and mortality. Turtles were assigned to one of three herbicide treatments or a control group. Turtles were exposed to atrazine, Roundup ProMax®, or Rodeo® via water bath during the first 3 weeks of the experiment. After 1 week, turtles were exposed to either a control (cell culture medium) or ranavirus-infected cell lysate via injection into the pectoral muscles. Necropsies were performed upon death or upon euthanasia after 5 weeks. Tissues were collected for histopathology and detection of ranavirus DNA via quantitative PCR. Only 57.5% of turtles exposed to ranavirus tested positive for ranaviral DNA at the time of death. Turtles exposed to ranavirus died sooner and lost more mass and carapace length, but not plastron length, than did controls. Exposure to environmentally relevant concentrations of herbicides did not impact infection rate, morbidity, or mortality of hatchling turtles due to ranavirus exposure. We also found no direct effects of herbicide or interactions with ranavirus exposure on growth or survival time. Results of this study should be interpreted in the context of the modest ranavirus infection rate achieved, the general lack of growth, and the unplanned presence of an additional pathogen in our study.

scholarly journals МОРФОЛОГІЧНІ, КУЛЬТУРАЛЬНІ ТА БІОХІМІЧНІ ВЛАСТИВОСТІ ІЗОЛЯТІВ БАКТЕРІЙ, ВИДІЛЕНИХ З ОРГАНІВ ЧЕРЕПАХИ ЧЕРВОНОВУХОЇ

2016 ◽  
Vol 18 (3(70)) ◽  
pp. 210-214
Author(s):  
M. Skripra ◽  
P. Saulin ◽  
I. Panikar ◽  
O. Machuskyy
Keyword(s):  
Klebsiella Pneumoniae ◽  
Yersinia Enterocolitica ◽  
Human Body ◽  
Staphylococcus Epidermidis ◽  
Culture Medium ◽  
Trachemys Scripta ◽  
Trachemys Scripta Elegans ◽  
Beef Extract ◽  
Highly Sensitive ◽  
Selective Culture Medium

The article presents the results of bacteriological tests of five Red-eared slider’s (Trachemys scripta elegans’) organs. Primary inoculations were made from heart, liver, kidney, spleen and lung on commonly used (beef-extract agar-agar and beef-extract broth) and selective culture medium (Endo agar, Xylose-Lysine Deoxycholate agar (XLD Agar), agar PALCAM, salt broths with varying concentrations of NaCl (2%, 5% and 10%)). Because of bacteriology tests from Red-eared slider’s (Trachemys scripta elegans’) organisms there were excreted four microorganisms’ isolates: two Staphylococcus isolates, and one of Klebsiella and one of Yersinia. We have found that strains of Klebsiella pneumoniae is highly sensitive to imipenem, meropenem, sensitive to amikacin, netilmicin. Strains of Yersinia enterocolitica are sensitive to amoxicillin, rifampicin, penicillin and ceftriaxone. Staphylococcus epidermidis (No. 1, 2, 4, 5) strains were highly sensitive to vancomycin and rifampicin, sensitive to gatifloxacin, linezolid. These strains have received PA-11/15 mark. Staphylococcus epidermidis (No. 3) had a sensitivity to methicillin. This difference in our opinion is fundamental, therefore, this strain was isolated and it received Poltava-15 mark. Taking into account that the isolates of Klebsiella pneumoniae and Yersinia enterocolitica selected are pathogenic for the human body and children are particularly sensitive to these pathogens, we consider it necessary to monitor the bacterial background of animals in pet shops and markets. 

scholarly journals Effects of Feed Mixed with Lactic Acid Bacteria and Carbon, Nitrogen, Phosphorus Supplied to the Water on the Growth and Survival Rate of White Leg Shrimp (Penaeus vannamei) Infected with Acute Hepatopancreatic Necrosis Disease Caused by Vibrio parahaemolyticus

Biology ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 280
Author(s):  
Linh Nguyen Thi Truc ◽  
Tuu Nguyen Thanh ◽  
To Tran Thi Hong ◽  
Day Pham Van ◽  
Minh Vo Thi Tuyet ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Survival Rate ◽  
Lactic Acid Bacteria ◽  
Vibrio Parahaemolyticus ◽  
Control Group ◽  
Penaeus Vannamei ◽  
Growth And Survival ◽  
Acute Hepatopancreatic Necrosis Disease ◽  
Nitrogen Phosphorus ◽  
Shrimp Health

This study aimed to evaluate the growth, survival rate, and resistance to acute hepatopancreatic necrosis disease (AHPND) of white leg shrimp (Penaeus vannamei) by using Lactobacillus plantarum, Lactobacillus fermentum, and Pediococcus pentosaceus mixed with feed, and at the same time supplying CNP in a ratio of 15:1:0.1 to the water. As a result, the treatments that shrimp were fed with feed containing lactic acid bacteria (LAB), especially L. plantarum, have increased shrimp growth, total hemocyte cells, granulocyte cells, and hyaline cells significantly (p < 0.05) in comparison to the control group. The supply of CNP to the water has promoted the intensity of V. parahaemolyticus effects on shrimp health and significantly decreased total hemocyte cells, granulocyte cells, and hyaline cells by 30–50% in the period after three days of the challenge, except in L. plantarum treatment, which had only a 20% decrease compared to other treatments. In CNP supplying treatments, the AHPND infected rate and mortality of shrimp were higher than those in other treatments. In summary, the supply of CNP had significantly reduced the shrimp’s immune response and promoted the susceptibility of shrimp to AHPND in both cases of use with and without LAB-containing diets.

scholarly journals Determination of Efficacy of Single and Double 4.7 mg Deslorelin Acetate Implant on the Reproductive Activity of Female Pond Sliders (Trachemys scripta)

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 660
Author(s):  
Edoardo Bardi ◽  
Martina Manfredi ◽  
Raffaella Capitelli ◽  
Emanuele Lubian ◽  
Alessandro Vetere ◽  
...  
Keyword(s):  
Phase Ii ◽  
Egg Production ◽  
Reproductive Activity ◽  
Trachemys Scripta ◽  
Phase Iii ◽  
Control Group ◽  
Sexual Hormones ◽  
Significant Difference ◽  
Group 6 ◽  
Plasmatic Concentration

The use of long-acting gonadotropin-releasing hormone (GnRH) agonists to suppress fertility has been poorly investigated in reptiles, and the few available studies show inconsistent results. The efficacy of single and double intramuscular 4.7 mg deslorelin acetate implants in captive pond sliders (Trachemys scripta) was investigated, with 20 animals divided into three groups: a single-implant group (6 animals), a double-implant group (6 animals), and a control group (no implant). During one reproductive season (March to October), plasmatic concentration of sexual hormones (estradiol, progesterone, and testosterone) and ovarian morphometric activity via computed tomography were monitored about every 30 days. A significative decrease in the number of phase II ovarian follicles was detected in the double-implant group compared with the control group, but no significant difference was noted in the number of phase III and phase IV follicles, egg production, and plasmatic concentration of sexual hormones. Results show that neither a single nor a double deslorelin acetate implant can successfully inhibit reproduction in female pond sliders during the ongoing season, but the lower number of phase II follicles in the double-implant group can possibly be associated with reduced fertility in the following seasons.

scholarly journals INABILITY TO INDUCE TYMPANIC SQUAMOUS METAPLASIA USING ORGANOCHLORINE COMPOUNDS IN VITAMIN A-DEFICIENT RED-EARED SLIDERS (TRACHEMYS SCRIPTA ELEGANS)

2008 ◽  
Vol 44 (3) ◽  
pp. 664-669 ◽  
Author(s):  
Karl R. Kroenlein ◽  
Jonathan M. Sleeman ◽  
Steven D. Holladay ◽  
Priscilla H. Joyner ◽  
Justin D. Brown ◽  
...  
Keyword(s):  
Vitamin A ◽  
Squamous Metaplasia ◽  
Trachemys Scripta ◽  
Organochlorine Compounds ◽  
Trachemys Scripta Elegans

Helminths from the red-eared slider Trachemys scripta elegans (Chelonia: Emydidae) in marshes from the eastern Iberian Peninsula: first report of Telorchis attenuata (Digenea: Telorchiidae)

2014 ◽  
Vol 28 ◽  
Author(s):  
Jesús Cardells ◽  
María Magdalena Garijo ◽  
Clara Marín ◽  
Santiago Vera
Keyword(s):  
Iberian Peninsula ◽  
Intestinal Tract ◽  
Trachemys Scripta ◽  
Trachemys Scripta Elegans ◽  
Emys Orbicularis ◽  
First Report ◽  
Digenean Trematode ◽  
Limited Sampling ◽  
Mauremys Leprosa ◽  
New Habitats

The present work describes the presence of a digenean in the red-eared turtle Trachemys scripta elegans (Wied-Neuwied, 1839) in marshes of the Valencian Community. The faeces and intestinal tract of 105 animals were examined. Only one helminth species was found and identified as the digenean trematode Telorchis atenuatta (Goldberger, 1911), present in the 7.6% of the animals analysed. This is the first report of the parasite in sliders from Spain. Although conclusions are preliminary due to the limited sampling, our results suggest that the presence of red-eared turtles in new habitats may increase the risk of introducing new microorganisms and new diseases with them, altering the sanitary status of the autochthonous terrapins Mauremys leprosa (Schweigger, 1812) and Emys orbicularis (Linnaeus, 1758).

A Prospective Trial of Prophylactic Povidone Iodine in Lacerations of the Hand

1985 ◽  
Vol 10 (3) ◽  
pp. 370-374
Author(s):  
A. H. N. ROBERTS ◽  
F. E. V. ROBERTS ◽  
R. I. HALL ◽  
I. H. THOMAS
Keyword(s):  
Wound Infection ◽  
Infection Rate ◽  
Adverse Reactions ◽  
Healing Rate ◽  
Povidone Iodine ◽  
Prospective Trial ◽  
Control Group ◽  
Iodine Treatment ◽  
Pain Patients

A series of 418 patients with lacerations of the hands were allocated randomly to a control group or to a group where the injury was treated with povidone iodine before suture. The incidence of infected and imperfectly healed wounds was determined seven days later. As well as the effect of povidone iodine on infection, thirteen other factors were also analysed. The overall infection rate of 5.0% and the 38.5% imperfect healing rate were not significantly affected by povidone iodine treatment, although both were reduced. The figures of four other trials were combined with this trial and this showed a significant effect of povidone iodine treatment. There were no adverse reactions to povidone iodine. It is therefore recommended that hand lacerations should be treated with povidone iodine prior to suture. Other factors found to be significantly important in wound infection or imperfect healing were the condition of the dressing, the part of the hand injured and pain. Patients should be strongly advised to keep their dressing clean and dry.

scholarly journals Dietary soybean meal on growth and intestinal morphology of South American catfish, Rhamdia quelen, larvae

2012 ◽  
Vol 42 (9) ◽  
pp. 1662-1668 ◽  
Author(s):  
David Roque Hernández ◽  
Juan José Santinón ◽  
Sebastián Sánchez ◽  
Hugo Alberto Domitrovic
Keyword(s):  
Soybean Meal ◽  
Control Diet ◽  
Intestinal Morphology ◽  
Control Group ◽  
South American ◽  
Growth And Survival ◽  
Posterior Intestine ◽  
Rhamdia Quelen ◽  
Anterior Intestine ◽  
Morphology Parameters

The effects of the replacement of bread yeast by soybean meal in diets for Rhamdia quelen larvae on growth, survival, and intestinal morphology were analysed. Larvae were fed for 20 days with five diets: a control diet formulated with 57% of bread yeast, and other four diets in which soybean meal at concentrations of 14.25, 28.5, 42.75 and 57% was added to obtain 25, 50, 75 and 100% of bread yeast replacement. Growth and survival parameters were negatively affected by dietary soybean meal inclusion. Larvae fed control diet showed significantly higher mean weight, specific growth rate, final biomass, and survival rate than larvae from other treatments. Enterocyte height and fold width of the posterior intestine showed highest values in the control group, and an inverse linear relationship with the level of dietary soybean meal inclusion was observed, however, in the anterior intestine the morphology parameters were not affected by the diet. These results indicate that inclusion of soybean meal in diets for R. quelen larvae negatively affects growth and survival, as well as the capacity for digestion and absorption of nutrients, mainly in the posterior intestine.

scholarly journals Antimicrobial effect of 2% sodium hypochlorite and 2% chlorhexidine tested by different methods

2003 ◽  
Vol 14 (1) ◽  
pp. 58-62 ◽  
Author(s):  
Carlos Estrela ◽  
Rosane Galhardo Ribeiro ◽  
Cyntia R.A. Estrela ◽  
Jesus Djalma Pécora ◽  
Manoel Damião Sousa-Neto
Keyword(s):  
Sodium Hypochlorite ◽  
Culture Medium ◽  
Agar Plate ◽  
Antimicrobial Effect ◽  
Control Group ◽  
Diffusion Test ◽  
Exposure Test ◽  
Agar Diffusion ◽  
Direct Exposure ◽  
Agar Diffusion Test

The objective of this study was to analyze the antimicrobial effect of 2% sodium hypochlorite (NaOCl) and 2% chlorhexidine (CHX) by agar diffusion test and by direct exposure test. Five microorganisms: Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, Bacillus subtilis, Candida albicans, and one mixture of these were used. These strains were inoculated in brain heart infusion (BHI) and incubated at 37ºC for 24 h. For the agar diffusion test (ADT), 18 Petri plates with 20 ml of BHI agar were inoculated with 0.1 ml of the microbial suspensions, using sterile swabs that were spread on the medium, obtaining growth in junction. Fifty-four paper disks (9 mm in diameter) were immersed in the experimental solutions for 1 min. Subsequently, three papers disks containing one of the substances were placed on the BHI agar surface in each agar plate. The plates were maintained for 1 h at room temperature, and then incubated at 37ºC for 48 h. The diameter of microbial inhibition was measured around the papers disks containing the substances. For the direct exposure test, 162 #50 sterile absorbent paper points were immersed in the experimental suspensions for 5 min, and were then placed on Petri plates and covered with one of the irrigant solutions, or with sterile distilled water (control group). After intervals of 5, 10 and 30 min, the paper points were removed from contact with the solutions and individually immersed in 7 ml of Letheen Broth, followed by incubation at 37ºC for 48 h. Microbial growth was evaluated by turbidity of the culture medium. A 0.1 ml inoculum obtained from the Letheen Broth was transferred to 7 ml of BHI, and incubated at 37ºC for 48 h. Bacterial growth was again evaluated by turbidity of the culture medium. Gram stain of BHI cultures was used for verification of contamination and growth was determined by macroscopic and microscopic examination. The best performance of antimicrobial effectiveness of NaOCl was observed in the direct exposure test, and of CHX was observed in the agar diffusion test. The magnitude of antimicrobial effect was influenced by the experimental methods, biological indicators and exposure time.

scholarly journals Standardising intraoperative irrigation with 0.05% chlorhexidine gluconate in caesarean delivery to reduce surgical site infections: A single institution experience

2019 ◽  
Vol 30 (1-2) ◽  
pp. 24-33
Author(s):  
Theresa Mangold ◽  
Erin Kinzel Hamilton ◽  
Helen Boehm Johnson ◽  
Rene Perez
Keyword(s):  
Treatment Group ◽  
Surgical Site Infection ◽  
Infection Rate ◽  
Caesarean Delivery ◽  
Chlorhexidine Gluconate ◽  
Control Group ◽  
Antibiotic Administration ◽  
Site Infection ◽  
Infection Rates ◽  
The Impact

Background Surgical site infection is a significant cause of morbidity and mortality following caesarean delivery. Objective To determine whether standardising intraoperative irrigation with 0.05% chlorhexidine gluconate during caesarean delivery could decrease infection rates. Methods This was a process improvement project involving 742 women, 343 of whom received low-pressured 0.05% chlorhexidine gluconate irrigation during caesarean delivery over a one-year period. Infection rates were compared with a standard-of-care control group (399 women) undergoing caesarean delivery the preceding year. Results The treatment group infection rate met the study goal by achieving a lower infection rate than the control group, though this was not statistically significant. A significant interaction effect between irrigation with 0.05% chlorhexidine gluconate and antibiotic administration time existed, such that infection occurrence in the treatment group was not dependent on antibiotic timing, as opposed to the control group infection occurrence, which was dependent on antibiotic timing. Conclusion Intraoperative irrigation with 0.05% chlorhexidine gluconate during caesarean delivery did not statistically significantly reduce the rate of infections. It did render the impact of antibiotic administration timing irrelevant in prevention of surgical site infection. This suggests a role for 0.05% chlorhexidine gluconate irrigation in mitigating infection risk whether antibiotic prophylaxis timing is suboptimal or ideal.

scholarly journals Pemanfaatan Tepung Biji Pepaya (Carica papaya) Terhadap Respons Pertumbuhan dan Tingkat Kelangsungan Hidup Ikan Nila (Oreochromis niloticus)

Jurnal MIPA ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 160
Author(s):  
Aprelia Martina Tomasoa ◽  
Deidy Azhari
Keyword(s):  
Survival Rate ◽  
Growth Performance ◽  
Energy Demand ◽  
Nile Tilapia ◽  
Feeding Rate ◽  
Control Group ◽  
Fish Feed ◽  
Growth And Survival ◽  
Papaya Seeds ◽  
Papaya Seed

Laju pertumbuhan dan tingkat reproduksi yang tinggi merupakan karakteristik unggul yang dimiliki ikan nila sebagai komoditi budidaya. Tingkat reproduksi yang tinggi menyebabkan terjadinya pematangan gonad sebelum mencapai ukuran produksi dimana hal tersebut dapat merugikan pembudidaya, karena saat ikan mengalami matang gonad energi hasil asimilasi pakan sebagain besar akan dialokasikan untuk perkembangan gonad dan menghambat pertumbuhan somatik. Pemanfaatan senyawa aktif dari bahan alami biji pepaya dapat menjadi solusi untuk meningkatkan pertumbuhan ikan yang dibudidayakan sekaligus menjaga keamanan pangan dan konsumen. Penelitian ini bertujuan untuk mengetahui pemanfaatan tepung biji pepaya terhadap respons pertumbuhan dan tingkat kelangsungan hidup ikan nila. Ikan nila diberi perlakuan dengan lima dosis tepung biji pepaya berbeda (0, 1, 3, 5 dan 7 g/kg pakan) secara oral berdasarkan feeding rate 5% dengan tiga ulangan selama 30 hari pemeliharaan. Hasil penelitian menunjukkan dosis 5 g/kg meningkatkan pertumbuhan bobot tubuh tertinggi sebesar 547 g dibandingkan kontrol sebesar 406 g. Sejalan dengan pertumbuhan bobot tubuh, dosis 5 g/kg menghasilkan tingkat kelangsungan hidup ikan nila sebesar 100% dibandingkan kontrol sebesar 66%. Dari hasil yang diperoleh dapat disimpulkan bahwa tepung biji pepaya dengan dosis 5 g/kg pakan dapat meningkatkan pertumbuhan dan tingkat kelangsungan hidup ikan nila selama 30 hariHigh growth rate and high reproductive cycle are some economical triats on nile tilapia. This is a setback for nile tilapia culture because when the fish reach gonad maturity before reach market size, all the energy demand will flow to reproduction and can cause stunt growth. The utilization of natural compound from papaya seeds can be a solution to enhance growth of farmed nile tilapia and for consumer and food safety. This research aim to know how papaya seed powder can enhance growth and survival rate of nile tilapia. This research conducted into five groups of 5 papaya seeds powder dosage (0, 1, 3, 5 and 7 g/kg) that feed orally to the treatment fishes. Result showed the fishes feed with 5 g/kg papaya seed experienced highest growth performance 547 g compare to control which is 406 g. Same as growth performance, survival rate shown that group feed with 5 g/kg papaya seed had 100% survival rate compare with control which is 66% during treatment periods. Base on this result, we can make conclusion that fish feed with 5 g/kg of papaya seed have a better growth performance and survival rate compare with control group for 30 days treatment periods

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