scholarly journals Detection of Jingmenviruses in Japan with Evidence of Vertical Transmission in Ticks

Viruses ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2547
Author(s):  
Daisuke Kobayashi ◽  
Ryusei Kuwata ◽  
Toshiya Kimura ◽  
Hiroshi Shimoda ◽  
Ryosuke Fujita ◽  
...  
Keyword(s):  
Vertical Transmission ◽  
Tick Species ◽  
Epidemiological Surveillance ◽  
Specific Manner ◽  
The Status ◽  
Transmission Cycles ◽  
Amblyomma Testudinarium ◽  
Species Specific ◽  
Collection Sites

Jingmen tick virus (JMTV) and the related jingmenvirus-termed Alongshan virus are recognized as globally emerging human pathogenic tick-borne viruses. These viruses have been detected in various mammals and invertebrates, although their natural transmission cycles remain unknown. JMTV and a novel jingmenvirus, tentatively named Takachi virus (TAKV), have now been identified during a surveillance of tick-borne viruses in Japan. JMTV was shown to be distributed across extensive areas of Japan and has been detected repeatedly at the same collection sites over several years, suggesting viral circulation in natural transmission cycles in these areas. Interestingly, these jingmenviruses may exist in a host tick species-specific manner. Vertical transmission of the virus in host ticks in nature was also indicated by the presence of JMTV in unfed host-questing Amblyomma testudinarium larvae. Further epidemiological surveillance and etiological studies are necessary to assess the status and risk of jingmenvirus infection in Japan.

scholarly journals Nationwide Temporal and Geographical Distribution of Tick Populations and Phylogenetic Analysis of Severe Fever with Thrombocytopenia Syndrome Virus in Ticks in Korea, 2020

2021 ◽  
Vol 9 (8) ◽  
pp. 1630
Author(s):  
Min-Goo Seo ◽  
Byung-Eon Noh ◽  
Hak Seon Lee ◽  
Tae-Kyu Kim ◽  
Bong-Goo Song ◽  
...  
Keyword(s):  
Tick Species ◽  
Temporal Distribution ◽  
Epidemiological Studies ◽  
Haemaphysalis Longicornis ◽  
Geographical Regions ◽  
Control And Prevention ◽  
Amblyomma Testudinarium ◽  
High Degree ◽  
Severe Fever ◽  
Minimum Infection Rate

Since 2010, the Korea Disease Control and Prevention Agency has established centers at 16 locations to monitor disease vectors and pathogens. Here, we examined tick populations to understand the geographical and temporal distribution of severe fever with thrombocytopenia syndrome virus (SFTSV) vectors in 2020. From April to November, 63,376 ticks were collected from traps to monitor tick populations, with a trap index of 41.3. Tick incidence varied from April to October, with population peaks observed for nymphs in May, adults in July, and larvae in September. The predominant tick species were Haemaphysalis longicornis, Haemaphysalis spp., H. flava, Ixodes spp., Amblyomma testudinarium, and Ixodes nipponensis. Approximately 50% of the collected ticks were pooled into 2973 groups to detect the rate of SFTSV infection in ticks. The minimum infection rate (MIR) of SFTSV was 0.2%, and Andong had the highest MIR for SFTSV (4.0%). The B3 genotype was the most prevalent (52.2%) followed by B2 (28.6%), B5 (15.9%), B4 (1.6%), and B6 (1.6%). We identified widely distributed tick species and a high degree of diversity in SFTSV strains in ticks from different geographical regions. The results may provide a basis for future epidemiological studies and risk assessments for tick-borne diseases.

scholarly journals Pig zona pellucida 2 (pZP2) protein does not participate in zona pellucida formation in transgenic mice

Reproduction ◽  
2006 ◽  
Vol 132 (3) ◽  
pp. 455-464 ◽  
Author(s):  
Akiko Hasegawa ◽  
Nozomi Kanazawa ◽  
Hideaki Sawai ◽  
Shinji Komori ◽  
Koji Koyama
Keyword(s):  
Extracellular Matrix ◽  
Transgenic Mice ◽  
Molecular Species ◽  
Zona Pellucida ◽  
Transgenic Protein ◽  
Specific Manner ◽  
Species Specific ◽  
Deficient Mice

The zona pellucida, an extracellular matrix surrounding mammalian oocytes, is composed of three or four glycoproteins. It is well known that the zona pellucida plays several critical roles during fertilization, but there is little knowledge about its formation. The purpose of this study is to examine whether a pig zona pellucida glycoprotein 2 (pZP2) would assemble with mouse zona pellucida. A transgene construct was prepared by placing a minigene encoding pZP2 downstream from the promoter of mouse ZP2. The result showed that the transgenic protein was synthesized in growing oocytes but not incorporated into the zona pellucida. Furthermore, the pZP2 transgene did not rescue the phenotype in ZP2-knockout zona-deficient mice. These results indicate that pZP2 does not participate in mouse zona pellucida formation and the zona pellucida is constituted from its component proteins in a molecular species-specific manner between mice and pigs.

Responses of Benthic Macroinvertebrate Species to Manipulation of Interstitial Detritus in Carnation Creek, British Columbia

1985 ◽  
Vol 42 (1) ◽  
pp. 139-146 ◽  
Author(s):  
Joseph M. Culp ◽  
Ronald W. Davies
Keyword(s):  
British Columbia ◽  
Microbial Activity ◽  
Tsuga Heterophylla ◽  
Benthic Macroinvertebrate ◽  
Alnus Rubra ◽  
Detritus Processing ◽  
Specific Manner ◽  
Species Specific ◽  
Macroinvertebrate Species ◽  
Trophic Feeding

Experimental manipulations were conducted in Carnation Creek, British Columbia, to determine the response of macroinvertebrate distribution and abundance to differences in detritus source and quantity. Four treatments (no detritus, low hemlock (Tsuga heterophylla), low alder (Alnus rubra), high alder) with a standardized substrate were established in a riffle and left for 28 d. Densities and/or biomasses of 12 of the 20 colonizing macroinvertebrate taxa were significantly different among the detritus treatments. Microbial activity, detritus processing, and macroinvertebrate abundances were highest in substrate patches with alder detritus. Compared with the no detritus and low hemlock treatments, the low alder treatment increased the abundances of nine taxa and decreased three taxa, while the high alder treatment increased the abundances of six taxa and decreased six taxa. Thus, detritus source and quantity strongly influenced macroinvertebrate community composition within the streambed. Although most of the macroinvertebrates in all treatments were either collector–gatherer or shredder detritivores, these macroinvertebrates responded to changes in detritus in a species-specific manner. Therefore, the Carnation Creek experiments emphasize the importance of interstitial detritus in the substrate as a factor that influences the microdistribution of the benthos at the species, rather than trophic feeding, assemblage level.

Endogenous galectin-3 controls experimental malaria in a species-specific manner

2012 ◽  
Vol 34 (7) ◽  
pp. 383-387 ◽  
Author(s):  
M. A. TOSCANO ◽  
J. E. TONGREN ◽  
J. B. De SOUZA ◽  
F.-T. LIU ◽  
E. M. RILEY ◽  
...  
Keyword(s):  
Specific Manner ◽  
Galectin 3 ◽  
Species Specific

The Effects of Cross-Fostering On the Behavior of Two Species of North American Lemmings, Dicrostonyx Groenlandicus and Lemmus Trimucronatus. Iii. Agonistic Behavior

Behaviour ◽  
1980 ◽  
Vol 73 (3-4) ◽  
pp. 261-275 ◽  
Author(s):  
Edwin M. Banks ◽  
U. William Huck
Keyword(s):  
Agonistic Behavior ◽  
Species Identity ◽  
Maternal Environment ◽  
Response Range ◽  
Dicrostonyx Groenlandicus ◽  
Specific Manner ◽  
Species Specific ◽  
Cross Fostering ◽  
Species Cross

Abstract1. The role of the maternal environment in development of species-specific agonistic behavior was investigated by reciprocally cross-fostering two species of lemmings (Discrostonyx groenlandicus and Lemmus trimucronatus). Non-fostered and within-species-fostered (in-fostered) animals served as controls. 2. When adult, cross-fostered male lemmings engaged in more frequent contact social behavior with the foster species than with conspecifics. However, since cross-fostered animals retained their ability to interact with conspecifics in a species-specific manner, cross-fostering appears to have resulted in a broadening of "species identity" to include the foster species. 3. In-fostering reduced the aggressiveness of Dicrostonyx but had little effect on the behavior of Lemmus. 4. Both the intensity and orientation of agonistic behavior were altered as a result of cross-fostering. In Dicrostonyx, a highly aggressive species, cross-fostering resulted in decreased aggression directed at the foster species and increased aggression toward conspecifics. In Lemmus, a less aggressive species, cross-fostering appears to have resulted in a broadening of the response range to stimuli provided by the foster species. When paired with aggressive Dicrostonyx, cross-fostered Lemmus engaged in more frequent and intense aggression than did controls. However, when paired with nonaggressive (in-fostered) Dicrostonyx, cross-fostered Lemmus engaged in less frequent and intense aggression than controls. In contrast, non-fostered and in-fostered Lemmus males had a relatively narrow response range to Dicrostonyx. 5. In general, the results suggested that agonistic behaviour in lemmings is the product of a complex interaction between genotype and maternal environment.

scholarly journals Respiratory Syncytial Virus (RSV) Fusion Protein Subunit F2, Not Attachment Protein G, Determines the Specificity of RSV Infection

2003 ◽  
Vol 77 (8) ◽  
pp. 4609-4616 ◽  
Author(s):  
Jörg Schlender ◽  
Gert Zimmer ◽  
Georg Herrler ◽  
Karl-Klaus Conzelmann
Keyword(s):  
Respiratory Syncytial Virus ◽  
Peripheral Blood Lymphocytes ◽  
Human Cells ◽  
Protein G ◽  
Human Beings ◽  
Protein Subunit ◽  
Attachment Protein ◽  
Specific Manner ◽  
Syncytial Virus ◽  
Species Specific

ABSTRACT Human respiratory syncytial virus (HRSV) and bovine RSV (BRSV) infect human beings and cattle in a species-specific manner. We have here analyzed the contribution of RSV envelope proteins to species-specific entry into cells. In contrast to permanent cell lines, primary cells of human or bovine origin, including differentiated respiratory epithelia, peripheral blood lymphocytes, and macrophages, showed a pronounced species-specific permissivity for HRSV and BRSV infection, respectively. Recombinant BRSV deletion mutants lacking either the small hydrophobic (SH) protein gene or both SH and the attachment glycoprotein (G) gene retained their specificity for bovine cells, whereas corresponding mutants carrying the HRSV F gene specifically infected human cells. To further narrow the responsible region of F, two reciprocal chimeric F constructs were assembled from BRSV and HRSV F1 and F2 subunits. The specificity of recombinant RSV carrying only the chimeric F proteins strictly correlated with the origin of the membrane-distal F2 domain. A contribution of G to the specificity of entry could be excluded after reintroduction of BRSV or HRSV G. Virus with F1 and G from BRSV and with only F2 from HRSV specifically infected human cells, whereas virus expressing F1 and G from HRSV and F2 from BRSV specifically infected bovine cells. The introduction of G enhanced the infectivities of both chimeric viruses to equal degrees. Thus, the role of the nominal attachment protein G is confined to facilitating infection in a non-species-specific manner, most probably by binding to cell surface glycosaminoglycans. The identification of the F2 subunit as the determinant of RSV host cell specificity facilitates identification of virus receptors and should allow for development of reagents specifically interfering with RSV entry.

Eucalypt phytoglyphs: The micronanatomical features of the epidermis in relation to taxonomy

1971 ◽  
Vol 19 (2) ◽  
pp. 173 ◽  
Author(s):  
SGM Carr ◽  
L Milkovits ◽  
DJ Carr
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Thin Sections ◽  
Hardening Process ◽  
Leaf Surfaces ◽  
The Status ◽  
Species Specific ◽  
High Degree ◽  
Scanning Electron

The eucalypt leaf contains a store of untapped information of potentially great value taxonomic and evututionary studies. Tie cuticie of certain eucalypts is shown to possess a complex and species-specific ornamentation so distinctive that its features can be regarded as diagnostic. The term "phytoglyph" is coined for the constellation of microanatomical features of the surfaces of leaves, including the microanatomy of the cuticle. Phytoglyphic analysis relates to the combination of three methods, light microscopy of stained cuticles, scanning electron microscopy of leaf surfaces, and light microscopy of thin sections of the cuticular and associated structures. Its use is illustrated by the dissection of the "form species" E. dichromophloia into a number of separate and recognizable entities, some of which were previously accorded the status of species. The plant geographical and other implications of this dissection are dealt with. In particular, E. dichromophloia F. Muell. is to be regarded as a species of very restricted distribution. The microanatomical characters of the cuticle are closely controlled products of the epidermal layers. The fact that specimens which (on other grounds) can be grouped together as a species have identical cuticular microanatomy suggests that the phytoglyph is genetically strongly determined and does not consist of inadvertent, trivial surface features with a high degree of plasticity. This in turn raises the problem of the development of the cuticular microanatomy which cannot be explained on current views of the formation of the cuticle by passive diffusion of precursor substances through the epidermal walls, followed by a hardening process.

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