Feed additive based on biologically active substances for fattening steers

2021 ◽  
pp. 58-65
Author(s):  
A. R. Farkhutdinova ◽  
M. T. Sabitov
Keyword(s):  
Amino Acids ◽  
Weight Gain ◽  
Trace Element ◽  
Feed Additives ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Daily Weight Gain ◽  
Feed Additive ◽  
Experimental Group ◽  
Active Substances

The modern agricultural market offers a wide range of products designed for a highly effective and most importantly safe increase in growth indicators, the level of development and the degree of productivity of domestic animals. The leading role in achieving the highest quality result is played by a competent choice of feed additives. The purpose of the research was to study the use of a feed additive based on biologically active substances (BAS) in the fattening of steers. The effect of the feed additive based on a natural, balanced, natural complex of biologically active substances such as vitamins B, C, PP, tryptophan (with oral administration bioavailability reaches 90 %), antioxidants, essential and free amino acids, enzymes and trace element salts on the growth and development of young cattle of Black-and-White breed has been studied. The steers of the control group received the main diet, and the animals of the experimental group in addition to the main diet received the feed additive consisting of vitamins, amino acids, antioxidants, enzymes and trace element salts. It has been found by the studies that the use of the test feed additive based on BAS provides an improvement in the digestibility and assimilation of nutrients in the diet, which contributed to the increase in the average daily live weight gain in the experimental group by 263 g or 25 %, the decrease in feed consumption for 1 kg of gain and the improvement in blood biochemical parameters, such as inorganic phosphorus, AST, ALT, alkaline phosphatase within physiological norms. In order to balance the diets of 9-monthold steers by biologically active substances and improve the physiological and biochemical status, the digestibility of nutrients that contribute to the increase in the average daily weight gain, it is recommended to use the feed additive based on BAS with drinking water.

COMPLEX FEED ADDITIVE TO NORMALIZE METABOLIC PROCESSES OF COWS

2021 ◽  
pp. 30-33
Author(s):  
Н.В. БОГОЛЮБОВА ◽  
Р.А. РЫКОВ
Keyword(s):  
The Body ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Feed Additive ◽  
Feed Supplement ◽  
Metabolic Processes ◽  
Activity Of Enzymes ◽  
Experimental Group ◽  
Active Substances ◽  
Alt Activity

Изучен способ нормализации обменных процессов и повышения продуктивности молочных коров при использовании комплекса биологически активных веществ. Эксперимент проведен на коровах голштинской породы (n=12) с продуктивностью за предыдущую лактацию 9000 кг молока. За 20 дней до отела и в течение 65 дней после коровы опытной группы получали комплексную кормовую добавку (ККД), включающую минерал шунгит, холин в «защищенной» форме, пробиотик «Целлобактерин+» и жмых льняной, в количестве 200 г на голову в сутки в смеси с концентратами. Для изучения обменных процессов в организме коров (n=5) в конце опыта отобраны пробы крови и определены основные биохимические показатели. Для оценки продуктивных качеств проводили контрольные дойки. Отмечено повышение в крови коров опытной группы концентрации общего белка на 2,8%, глюкозы — на 26,8% (P≤0,01), фосфолипидов — на 15,2% (P≤0,05), снижение мочевины на 23,6% (P≤0,05), билирубина — на 23,8% (P≤0,01), активности АЛТ на 13,11 (P≤0,05), АСТ — на 14,6% (P≤0,05). Использование ККД позволило снизить и привести в норму активность ферментов: лактатдегидрогеназы (ЛДГ), гаммаглутаминтрансферазы (ГГТ), креатинкиназы. Повышение интенсивности обменных процессов и нормализация показателей, характеризующих работу печени с использованием в питании комплекса биологически активных веществ ферментно-пробиотического, антиоксидантного и липотропно-гепатопротекторного действия, привело к повышению молочной продуктивности. A method of normalizing metabolic processes and increasing the productivity of dairy cows using a complex of biologically active substances has been studied. The experiment was carried out on Holstein cows (n = 12) with a productivity for the previous lactation of 9000 kg of milk. 20 days before calving and within 65 days after the cows of the experimental group received a complex feed supplement (CFS), including the mineral shungite, choline in a "protected" form, the probiotic "Cellobacterin +" and flaxseed cake, in an amount of 200 g per head per day mixed with concentrates. To study metabolic processes in the body of cows (n = 5), at the end of the experiment, blood samples were taken and the main biochemical parameters were determined. To assess the productive qualities, control milking was carried out. An increase in the blood of cows of the experimental group was noted in the concentration of total protein by 2.8%, glucose - by 26.8% (P≤0.01), phospholipids — by 15.2% (P≤0.05), a decrease in urea by 23,6% (P≤0.05), bilirubin - by 23.8% (P≤0.01), ALT activity by 13.11 (P≤0.05), AST — by 14.6% (P≤ 0.05). The use of CFS made it possible to reduce and normalize the activity of enzymes: lactate dehydrogenase (LDH), gamma glutamine transferase (GGT), creatine kinase. An increase in the intensity of metabolic processes and the normalization of indicators characterizing the work of the liver with the use of a complex of biologically active substances of enzymatic-probiotic, antioxidant and lipotropic-hepatoprotective action in the diet led to an increase in milk productivity.

OPTIMIZATION OF THE CONTENT OF A MULTICOMPONENT ADDITIVE ON BASED OIL COMPOSITION OF BIOLOGICALLY ACTIVE SUBSTANCES AND A PROBIOTIC

2020 ◽  
Author(s):  
М.В. ЛУКЬЯНЕНКО ◽  
Р.В. КАЗАРЯН ◽  
Т.В. ВАНИЦКАЯ ◽  
Е.В. КУЗЬМИНОВА ◽  
М.П. СЕМЕНЕНКО ◽  
...  
Keyword(s):  
Enterococcus Faecium ◽  
Feed Additives ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Farm Animals ◽  
Feed Additive ◽  
Control Group ◽  
Oil Composition ◽  
Active Substances

Для интенсификации производства животноводческой продукции и повышения ее качества необходимо обогащать рацион сельскохозяйственных животных и птицы кормовыми добавками, содержащими комплекс биологически активных веществ. Исследованы образцы комплексной кормовой добавки, содержащей масляную композицию БАВ в-каротина, витаминов Е и С, жидкого рапсового лецитина и селена, и пробиотическую добавку (ПД) Целлобактерин в соотношениях соответственно от 1 : 1 до 1 : 5. Установлено, что при соотношении масляная композиция БАВ : ПД Целлобактерин 1 : 3 количество пробиотической культуры Enterococcus faecium 1-35, содержащейся в ПД Целлобактерин, в опытном образце достигает максимального значения 6,4 Ч 105 КОЕ/г. При этом обеспечивается равномерное распределение масляной композиции БАВ на поверхности ПД. В опытах in vivo определена эффективность применения комплексной добавки с установленным соотношением компонентов для нормализации обменных процессов в организме животных. Доказано, что введение в рацион лабораторных крыс опытной группы многокомпонентной кормовой добавки в количестве 3 к основному рациону (ОР), контаминированному микотоксинами, позволяет достичь опережения прироста массы тела на 15-е и 45-е сутки эксперимента на 6,45 и 33,05 соответственно снижения содержания мочевины в сыворотке крови на 58,43 по сравнению с аналогичными показателями у животных контрольной группы, получавших только ОР. Степень снижения аланинаминотрасферазы и аспартатаминотрансферазы по отношению к аналогичным показателям контрольной группы составила 18,53 и 29,89 соответственно. Следовательно, комплексная кормовая добавка при соотношении масляная композиция БАВ : ПД Целлобактерин 1 : 3 обладает ярко выраженными гепатопротекторными и антитоксическими свойствами. Enriching the diet of farm animals and poultry by feed additives containing a complex of biologically active substances is necessary to intensify the production of livestock products and improve their quality. Samples of a complex feed additive containing an oil composition of BAS в-carotene, vitamins E and C, liquid rapeseed lecithin and selenium, and a probiotic additive (PA) Cellobacterin in ratios from 1 : 1 to 1 : 5 respectively were studied. It was found that the ratio of the oil composition of BAS : PA Cellobacterin 1 : 3 respectively the amount of probiotic culture Enterococcus faecium 1-35 contained in PA Cellobacterin in the experimental sample reaches a maximum value 6,4 Ч 105 CFU/g. In this case, the distribution of BAS oil composition on the PA surface is uniform. The effectiveness of using a complex additive with a set ratio of components to normalize metabolic processes in animals was determined in in vivo experiments. It is established that introduction in a diet of laboratory rats of the experimental group multicomponent feed additive in an amount of 3 to the basic ration (BR), contaminated with mycotoxins, can achieve compared to the same indicators in animals of the control group who received only the BR, the timing of weight gain on 15-th and 45-th day of the experiment by 6,45 and 33,05 respectively reduction of urea content in blood serum at 58,43. The degree of reduction of alanine aminotransferase and aspartate aminotransferase in relation to similar indicators of the control group was 18,53 and 29,89 respectively. Therefore, a complex feed additive with the ratio of oil composition of BAS : PA Cellobacterin 1 : 3 has pronounced hepatoprotective and antitoxic properties.

scholarly journals System of antioxidant protection of the body of piglets under the action of feed additive “Sylimevit”

2021 ◽  
Vol 23 (104) ◽  
pp. 10-17
Author(s):  
L. V. Vyslotska ◽  
B. V. Gutyj ◽  
O. V. Kozenko ◽  
V. I. Khalak ◽  
M. V. Chornyj ◽  
...  
Keyword(s):  
Body Weight ◽  
Antioxidant Defense System ◽  
Feed Additives ◽  
The Body ◽  
Biologically Active ◽  
Feed Additive ◽  
Free Access ◽  
Direct Acting ◽  
Active Substances ◽  
Technological Stress

One of the conditions for obtaining high-quality pig products is the use of feed additives, which contain all the necessary biologically active substances, eliminating their deficiency in feed and acting as catalysts for metabolic processes in pigs. That is why the aim of the study was to study the effect of feed additive “Sylimevit” on the enzymatic and non-enzymatic parts of the antioxidant system of piglets after weaning. Two groups of piglets were formed – control and experimental, in the amount of 10 individuals in each group, selected on the principle of analogues – age, breed and body weight. During the weaning period, the piglets were kept under the sow in special machines, had constant access to the mother, and from the age of 5 days – free access to concentrated feed. Feeding of animals was carried out in accordance with the norms for a given age of pigs. Prior to the study, a clinical and physiological examination of the piglets was performed. Their general condition and activity when eating food were taken into account. On the 28th day of life, the piglets were weaned from the sow and regrouped from different nests in order to be further maintained during the period of fattening and rearing with a change in the structure of the diet, which served as technological stress for the animals. From the age of 5 days, piglets of all groups were fed pre-starter feed. Piglets of the experimental group, from 21 to 40 days of age, were additionally fed the feed supplement “Sylimevit” at a dose of 100 mg/kg body weight per day. Feeding piglets Silimevit feed enhances their antioxidant status. The use of this feed additive in piglets contributed to the activation of both enzymatic and non-enzymatic parts of the antioxidant defense system of piglets after weaning, as indicated by an increase in blood 35-day-old piglets superoxide dismutase activity by 26.9 % (P < 0.001), catalase – 41.1 % (P < 0.001), glutathione peroxidase – by 52.7 % (P < 0.05), glutathione reductase – by 53.5 % (P < 0.05), the content of reduced glutathione – by 75 % (P < 0.001). This is due to the fact that silimevit contains active substances such as milk thistle and vitamins, which are strong direct-acting antioxidants and directly interact with free radicals and reactive oxygen species.

scholarly journals The productivity and mass of internalss of quails are for actions feed addition of «Proenzym»

2016 ◽  
Vol 18 (2) ◽  
Author(s):  
N. M. Baluh
Keyword(s):  
Live Weight ◽  
Maximum Amount ◽  
Feed Additives ◽  
Biologically Active ◽  
Poultry Feed ◽  
Feed Additive ◽  
Internal Organs ◽  
Basic Diet ◽  
Experimental Group ◽  
Different Doses

Established that the largest distribution combined with feed additives which include a number of biologically active substances. As an alternative to antibiotics, the use of which recently banned their production and significantly reduced feeding system is used four groups of drugs, enzymes, probiotics, prebiotics and аcidifiers. The largest number of feed additives used in poultry feed. Therefore, to improve the consumption and use of feed efficiency, obtaining maximum animal performance ensured a high level of balanced nutrition using the same enzyme–probiotic supplements. It is to include such feed additives «Proenzym» which consists of live bacteria genus Bacillus subtilis and an enzyme tsellyulaza. What prevents gastrointestinal diseases, improves digestion and absorption of essential nutrients intake. Therefore, the aim was to study the effect of feeding different doses feed additive «Proenzym» in the quail Full–feed and its impact on productivity and internal organs. For the experiment selected 200 day old Estonian quail breed. Of these, the principle of analogues formed four groups of 50 goals each. The duration of the experiment – 56 days. The first (control) group during the entire experiment received a basic diet, and quail research groups in addition to the basic diet fed different doses of feed additives. For the effects of various doses feed additive had a positive effect on live weight quails in 42 and 49–day age. Yes, most live weight at the end of the experiment were females and males second experimental group 10.8% and 8.3%, which in addition to full–feed introduced minimum dose «Proenzym» The introduction of the new maximum amount of feed factor had a significant impact on the weight of internal organs, quails second group for the actions of study drug increased the mass of the lungs and liver, respectively, 0.6 g and 3.4 g, and gallbladder in 4 experimental group 0.16 g. Therefore, we can conclude that the diets of quails best was the maximum amount of feed additive.

scholarly journals The efficiency enzyme preparation and feed additive cfa 10 use for pigs feeding

2016 ◽  
Vol 18 (2) ◽  
Author(s):  
G. M. Ogorodnichuk
Keyword(s):  
Enzyme Preparation ◽  
Negative Influence ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Feed Additive ◽  
Daily Growth ◽  
Balanced Diet ◽  
Manufacturing Company ◽  
Fattening Pigs ◽  
Active Substances

The three component enzyme preparation produced by Ladyzhyn scientific and manufacturing company «Enzym» was used in our research; this preparation consists of amilosubtylin, protosubtylin and pectinase. The diets balance was achieved by feed additive CFA 10, its recipe was created by Fodder research institute of UAAS and Trypilskyy biochemical plan of Obukhiv district, Kyiv region. He usage of three component enzyme preparation and feed additive CFA 10 with diets with some unbalanced ingredients facilitates increasing average daily growth; it give the possibility to save some deficient feeds without negative influence on slaughter characteristics and internal weight. The feeding of fattening pigs by diets with feed additive CFA 10 aimed to be balanced by some biologically active substances provides the increasing of growth by 19.2% and decreases the feed units charges by 22.7%. The additional usage of three component enzyme preparation with balanced diet by all ingredients does not facilitate the pigs efficiency and does not increase their slaughter qualities.

BACKGROUND CONTENT OF TECHNOGENIC AND NATURAL RADIONUCLIDES IN DETOXIFYING AND BIOLOGICALLY ACTIVE SUBSTANCES

2021 ◽  
Vol 1 (2) ◽  
pp. 223-229
Author(s):  
G.А. Zhorov ◽  
◽  
L.L. Zakharova ◽  
V.N. Obryvin ◽  
◽  
...  
Keyword(s):  
Specific Activity ◽  
Raw Materials ◽  
Natural Radionuclides ◽  
Animal Husbandry ◽  
Maximum Level ◽  
Feed Additives ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Radioactive Isotopes ◽  
Active Substances

For correctly assess the effectiveness and safety of the use of sorption-detoxifying agents and feed additives intended for animals receiving feed with an excess content of toxicants, it is necessary to take into account the background levels of radionuclides, toxic elements, pesticides and other technogenic and natural pollutants in the studied substances. The need for such studies is due both to the existence of areas with a naturally elevated level of natural toxicants in the sources of raw materials for the production of additives and drugs, and to the increasing anthropogenic influence, accompanied by the entry of xenobiotics into environmental objects. In series of radiometric studies, the specific activity of technogenic and natural radioactive isotopes (90Sr, 137Cs, 40К, 226Ra, 232Th) in a number of sorption-detoxifying and biologically active substances and preparations used in animal husbandry and veterinary medicine as part of feed additives and pharmacological agents was determined. It was found that in 43% of the studied samples, the level of specific activity of 1,1Sr in 3-90 times higher than allowed by the current standards. The maximum level of 90Sr, equal to (137±9) Bq/kg, was detected in perlite. The specific activity of 137Cs did not exceed the permissible level: in mineral sorbents its amount reached (40±7) Bq/kg, in organic and complex sorbents – (24±4), and in feed additives – (29±8) Bq/kg. The maximum levels of 40K were (1429±83) Bq/ kg in minerals (radionite) and (2613±100) Bq/kg in organic substances (lignohumate). The levels of 226Ra and 232Th did not exceed (153±13) and (79±13) Bq/kg, respectively, and were higher in the mineral samples.

scholarly journals EFFECT OF BIOLOGICALLY ACTIVE SUBSTANCES FORMED BY BACCILUS COAGULANS TI ON THE GROWTH OF CROPS

2013 ◽  
Vol 17 (2) ◽  
pp. 53-60
Author(s):  
Tatiana Lisitskaya ◽  
Tatiana Trosheva
Keyword(s):  
Amino Acids ◽  
Plant Biomass ◽  
Bacillus Coagulans ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Submerged Cultivation ◽  
Peptone Medium ◽  
Active Substances ◽  
Stimulation Of

Abstract The influence of cultural liquid of bacteria, Bacillus coagulans TI on the growth of several crops are studded. It was established that during the submerged cultivation on glucose-peptone medium these bacteria form gibberellins and amino acids. Pre-sowing treatment of seeds with diluted cultural liquid causes the stimulation of plants growth and improves the quality of plant biomass.

scholarly journals Veterinary and sanitary characteristics of quail meat by feeding nanocrystalline cerium dioxide

2021 ◽  
pp. 27-36
Author(s):  
V. Zotsenko ◽  
V. Dzhmil ◽  
D. Ostrovskiy ◽  
A. Andriichuk ◽  
T. Melnyk
Keyword(s):  
Drinking Water ◽  
Volatile Fatty Acids ◽  
Feed Additives ◽  
Biologically Active ◽  
Feed Additive ◽  
Average Weight ◽  
Free Access ◽  
Biological Value ◽  
Experimental Group

The unresolved problem of quail is feed stress and high sensitivity of poultry of this species to feed quality. To eliminate such consequences it is possible with the use of various biologically active feed additives. However, their introduction into the diet automatically raises questions about the quality and safety of the products for the consumer. The purpose of the research is to conduct a veterinary and sanitary assessment of the quality of quail meat for feeding NDC. The purpose of the research is to conduct a veterinary and sanitary assessment of the quality of quail meat for feeding NDC. The object of study were the quails of the Pharaoh breed, at the age of 24 were divided into two groups: experimental and control 30 heads each. The birds were kept in cages with free access to food and water. Quails of the experimental group were added to drinking water feed additive "Nanocerium" at a dose of 8.6 mg per liter of drinking water. Nanocerium feed additive is an aqueous dispersion of NDC with average nanoparticle sizes of 2–7 nm. Antemortem examination of quails of both groups revealed a satisfactory clinical condition of birds. The average weight of the gut carcass of quails of the experimental group was higher by 8.3% compared to the control. Examination of 20 carcasses of quails showed that they can be classified as first class. According to organoleptic parameters, quail meat is of good quality in veterinary and sanitary terms. Microbiological studies of quail meat indicate no effect of NDC in the applied doses on its bacterial contamination. Chemical parameters of meat (pH, aminoammonia nitrogen, volatile fatty acids during storage in the refrigerator) (5 days, t = 4–5 ° C) tended to increase and were within the normal range for fresh product. Tissue in quails of both groups was not detected. The biological value of quail meat in both groups was identical and no toxicity. Tasting evaluation of the broth and meat showed that NDC did not affect the studied taste. The obtained results show that the quails that received the feed additive "Nanocerium" with water according to the indicators of veterinary and sanitary examination are of good quality, which allows to use it in human food without restrictions. Key words: poultry, nanocerium, organoleptic evaluation, microbial contamination, biological value, tasting evaluation.

scholarly journals Chemical Composition and Content of Biologically Active Substances Found in Cotinus coggygria, Dactylorhiza maculata, Platanthera chlorantha Growing in Various Territories

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2806
Author(s):  
Stanislav Sukhikh ◽  
Lyudmila Asyakina ◽  
Maxim Korobenkov ◽  
Liubov Skrypnik ◽  
Artem Pungin ◽  
...  
Keyword(s):  
Trace Elements ◽  
Capillary Electrophoresis ◽  
Medicinal Plants ◽  
Feed Additives ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Livestock Products ◽  
Cotinus Coggygria ◽  
Active Substances

Medicinal plants (Cotinus coggygria, Dactylorhiza maculata, Platanthera chlorantha) growing in various territories (Kaliningrad, Moscow, and Minsk regions) were the objects of research. This paper presents a study of the chemical composition of these plants. To analyze the qualitative and quantitative composition of biologically active substances, the method of high-performance liquid chromatography was used. Atomic absorption spectrometry was used to study the content of trace elements. The content of organic acids and vitamins was determined by capillary electrophoresis using the Kapel-105/105M capillary electrophoresis system with high negative polarity. Extracts of medicinal plants were obtained on a Soxhlet apparatus using 70% ethanol as an extractant. It was found that among the biologically active substances in the plants under discussion, hyperoside, rutin (C. coggygria), Ferulic acid and Gallic acid (D. maculata), triene hydrocarbon (3,7-Dimethyl-1,3,6-octatriene), unsaturated alcohol (3,7-Dimethyl-2,6-octadien-1-ol), and benzyl acetate (P. chlorantha) prevailed. Samples of these medicinal plants contained trace elements (phosphorus, potassium, calcium, sodium, magnesium, and sulfur) and many aliphatic organic acids (succinic acid, benzoic acid, fumaric acid, citric acid, oxalic acid, and tartaric acid). The largest amount of biologically active substances and secondary metabolites of the studied plants from the Eastern Baltic is associated with climatic and ecological differences from other regions. The composition of these plants determines the potential of their use in feed additives for livestock and poultry as part of measures to improve the quality of livestock products. The use of medicinal plants for the production of feed additives is relevant in terms of improving regional economies, as well as improving the quality of life and nation’s health by providing ecologically clean livestock products.

scholarly journals Study of the Properties of In Vitro Dactylorhiza maculata (L.) Soó (Family Orchidaceae) Extracts

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1330
Author(s):  
Stanislav Sukhikh ◽  
Svetlana Noskova ◽  
Svetlana Ivanova ◽  
Liubov Skrypnik ◽  
Artem Pungin ◽  
...  
Keyword(s):  
Antioxidant Properties ◽  
Antimicrobial Properties ◽  
Feed Additives ◽  
Water Soluble ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Bioactive Substances ◽  
Limit Values ◽  
Active Substances

The medicinal plant Dactylorhiza maculata (L.) Soó (family Orchidaceae) is used to treat gastritis, colic, gastrointestinal tract, and bladder diseases. This study aimed to investigate the properties and characteristics of the in vitro Dactylorhiza maculata extract. The recommended parameters for producing Dactylorhiza maculata extract were determined: temperature 60 °C, process duration 60 min, hydro module 1:10. It is recommended to carry out the extraction using an aqueous ethyl alcohol solution with a mass fraction of the parent substance of 70%. It was found that such biologically active substances as rutin, quercetin, 3,3’,4’,5,5’,7-hexahydroxyflavonone, 3,3’,4’,5,5’,7-hexahydroxyflavonone-3-O-glycoside, gallic acid, and ferulic acid were dominant in Dactylorhiza maculata. A high phosphorus content was noted (2410.8 mg/kg dry matter). The studied Dactylorhiza maculata samples contained a large number of organic acids and water-soluble vitamins. The tested extracts were safe in terms of the content of heavy metals, pesticides, aflatoxin B1, and radionuclides, as well as pathogenic and opportunistic microorganisms; the content was significantly lower than the threshold limit values. The studied complex of biologically active substances from Dactylorhiza maculata extract samples had antimicrobial properties. It was found that the antioxidant activity of the samples was 217.89 ± 10.89 mg AA/g (AA—ascorbic acid). The high content of bioactive substances and the antimicrobial and antioxidant properties of Dactylorhiza maculata extract samples determine the application potential of this plant as a substitute for growth stimulants and feed antibiotics in the production of feed additives, aiming to increase the physiological and immune status of livestock and poultry.

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