scholarly journals Investigation of bacteria liable for Urinary Tract Infections and their antibiogram analysis

2018 ◽  
pp. 26-35

Urinary tract infection mainly occurs by microorganism when they overcome the human immune system. These microbes have caused the high rate of morbidity and motility effect all over the world to every age and gender. Lower urinary tract infection is more common among females than males. A prospective observational study was conducted in Pathology Department at Lady Reading Hospital Medical Teaching Institution, Peshawar, Pakistan in a 4th quarter (October, November, December) of the year 2017. Mid-stream urine samples were collected from 1357 patients from all hospital-admitted and outdoor patients. They were cultured on CLED agar, after isolation, uropathogens was identified through biochemical tests like oxidase, catalase and TSI tests. Only 205 samples were reported positive, with the prevalence of 15.10%. Females (51.70%) were more prone to UTI then males (48.29%). E.coli was most frequently isolated uropathogen with frequency of 90.24%. Other isolates were Pseudomonas aeruginosa (4.8%), Enterococcus faecium (2.92%), Enterobacter species (1.46%) and Proteus mirabilis (0.49%). Thirteen antibiotics were used to test the susceptibility of isolated microbes through Kirby Bauer Disc Diffusion method. Carbapenem drug like Imipenem (89.7%) was most effective against gram-negative microbes belonging to Enterobacteriaceae family. Other antibiotics like, Piperacillin + Tazobactam (84.86%), Cefoperazone /Sulbactam (84.3%), Amikacin (84.3%), Fosfomycin (83.7%) and Nitrofurantoin (77.2%) also showed sensitivity. Fluoroquinolones Drugs like Ciprofloxacin (82.1%) and Levofloxacin (81.6%) showed high resistivity followed by Co-trimoxazole (80.54%) and Cephalosporine drugs. For Gram positive Enterococcus spp, Glycopeptide antibiotics and protein synthesis inhibitor drugs had shown 100% sensitivity, but carbapenem and fluoroquinolones were highly resistant. Keywords: Enterobacteriaceae Family, Urinary Tract Infection (UTI), Fluoroquinolones Drug

Author(s):  
Elham Rezaee ◽  
Seyed Adnan Kashfi ◽  
Parisa Bagheri

Background and Aims: Urinary tract infections are one of the most common human infections seen in all age groups and both sexes. Inappropriate use of antibiotics to treat urinary tract infection causes the resistance of the pathogens to the drug. The present study aimed to determine the frequency of gram-negative and gram-positive bacteria and antibiotic resistance patterns in patients with urinary tract infection. Materials and Methods: Samples were cultured on Blood Agar and Eosin Methylene Blue. Colonieschr('39') growth was identified by biochemical tests and standard microbiological and antibiotic sensitivity tests, which were performed with the disc diffusion method according to the Clinical and Laboratory Standards Institute 2016 Standard. Results and Conclusion: The isolated bacteria showed the highest susceptibility to imipenem (89.66%) and meropenem (87.21%) and the highest resistance to sulfamethoxazole (50.00%) and nalidixic acid (44.09%). So, using imipenem is recommended as the most effective antibiotic for the treatment of infection.


2020 ◽  
Vol 7 (2) ◽  
Author(s):  
Shima Sadat Lesani ◽  
Mohammad Soleimani ◽  
Pegah Shakib ◽  
Mohammad Reza Zolfaghari

Background: Escherichia coli is considered as one of the causes of opportunistic infections. Nowadays, due to the increase in drug resistance, the treatment of these infections has become very difficult and they are recognized as the main causes of death in hospitalized patients. Objectives: The aim of this study was to determine the prevalence of blaTEM, blaSHV, and blaCTX-M genes in E. coli strains isolated from the urinary tract infection in patients in Intensive Care Units of three different hospitals in Qom, Iran. Methods: This study was conducted in three months from October to December 2014. A total of 200 E. coli samples were taken from the patients with urinary tract infections in Intensive Care units of Qom hospital. The disc diffusion method was used to determine the susceptibility pattern of antibiotic and phenotypic confirmatory tests for screening of the expanded spectrum beta-lactamase (ESBL) isolates. The presence of blaTEM, blaSHV, and blaCTX-M genes was evaluated by the polymerase chain reaction (PCR) assay. Results: Of 200 samples, ampicillin (96%) and nitrofurantoin (19.5%) showed the highest and lowest drug resistance, respectively. A total of 156 isolates (78%) were identified as ESBLs using the phenotypic method. Moreover, 76 (38%), 90 (45%), and 123 (61.5%) isolates consisted of blaCTX-M, blaSHV, and blaTEM, respectively. Conclusions: Overall, the findings of this study showed that blaTEM was the most common gene with a frequency of 61.5% in ESBL E. coli.


2017 ◽  
Vol 11 (1) ◽  
pp. 292-300 ◽  
Author(s):  
Angus N. Oli ◽  
Vivian B. Akabueze ◽  
Chijioke E. Ezeudu ◽  
George U. Eleje ◽  
Obiora S. Ejiofor ◽  
...  

Background: Urinary Tract Infection (UTI) is a common contagion among men and women with the incidence relatively higher among women due to their differing anatomy. An understanding of the kind of pathogens implicated in urinary tract infections as well as antibiotic susceptibility profiling may help the clinician make rationally correct empirical choice in their treatment. Objective: This study is aimed at determining the type and antibiotic susceptibility pattern of bacterial uropathogens isolated from female patients attending Chukwuemeka Odumegwu Ojukwu University Teaching Hospital (COOUTH), Awka, Nigeria. Method: Two hundred and forty patients with clinically diagnosed UTI and who were on at least 5 days’ antibiotic holiday were recruited into the study. Their demographic characteristics were captured using pre-tested questionnaire. Their clean catch mid-stream urine samples were collected using sterile universal container and sent to the Microbiology Department for processing. Within 30 minutes of samples collection, the specimens were cultured and the isolates were identified, after 24 h of incubation, using standard microbiological techniques. Antibiotic susceptibility tests were done with standard antibiotic discs using the Kirby–bauer disc diffusion method. Results: Out of the 240 urine samples, 89.17% yielded significant bacteriuria. The pathogens implicated were Escherichia coli (28.5%), Staphylococcus aureus (28.0%), Salmonella spp (22.8%) and Pseudomonas aeruginosa (20.5%). HIV status, patients age, pregnancy status and marital status all significantly affected bacteriuria rate (p value < 0.05), while patients’ location (sub-urban/rural dwelling), and level of education did not (p value > 0.05). The pattern of microbial resistance to antibiotics suggests that ceftazidime, fosfomycin and cefoxitin may not be used as first-line agents in the empirical treatment of UTIs rather; levofloxacin, meropenem or aztreonam should be considered. Levofloxacin was significantly effective against all the isolates and may be administered empirically while waiting for the culture result (Mean % susceptibility was 79.85). Conclusion: E. coli and S. aureus were the predominant pathogens in the study and many were resistant to the commonly prescribed antibiotics and so leave the clinicians with only few alternative drugs for UTIs treatment. Routine surveillance and monitoring studies need to be constantly conducted to update clinicians on the prevalent pathogens and the rational and empirical treatment of UTIs. Aggressive and consistent health education using every possible media is also recommended to combat the menace of drug resistance occasioned by inappropriate antibiotic use.


2013 ◽  
Vol 2 (3) ◽  
pp. 82-86 ◽  
Author(s):  
G Gautam ◽  
S Regmi ◽  
NT Magar ◽  
B Subedi ◽  
T Sharma ◽  
...  

INTRODUCTION: Urinary tract infection (UTI) is considered as the most common bacterial infectious disease seen among the pediatric patients. Most commonly, members of Enterobacteriaceae, particularly uropathogenic strains of E. coli and Enterobacter spp. are the primary causative pathogens of UTI in the different part of the world. Emergence of antimicrobial resistance rates among pathogens recovered from urinary tract infections is an increasing problem in the specific region. MATERIALS AND METHODS: Prevalence and anti microbial susceptibility pattern of the bacterial uropathogens isolated from the children attending Gandaki Medical College Teaching Hospital and Reserch Center (GMC) Nepal. A total of 155 children aged upto 15 years were included in this study. Urine cultures were carried out and the isolates were identified by Gram staining and conventional biochemical methods. Antimicrobial susceptibility testing was performed by disk diffusion method according to the Clinical and Laboratory Standards Institute (CLSI). RESULTS: In the present study 21.3 % of the sample size, showed significant bacterial growth. E. coli was the most frequently occurring pathogen (39.40%), followed by Proteus spp. 21.2%, Citrobacter spp. and Streptococcus faecalis (12.1%) Klebsiella spp. (9.1%), and Staphylococcus aureus and Enterobacter (3.0%). Susceptibility rate of E. coli were 69.2% to Gentamycin and Amikacin, 53.8% to Norfloxacin, 38.4% to Nalidixic acid and Norfloxacin. CONCLUSIONS: Pediatric urine culture isolates were becoming increasingly resistant to commonly used antibiotics. Finally, we suggest that empirical antibiotic selection should be based on knowledge of the local prevalence of bacterial organisms and antibiotic sensitivities rather than on universal guidelines. DOI: http://dx.doi.org/10.3126/ijim.v2i3.8665   Int J Infect Microbiol 2013;2(3):82-86


2016 ◽  
Vol 5 (2) ◽  
pp. 51-55 ◽  
Author(s):  
Bigu Kumar Chaudhari ◽  
Ganesh Kumar Singh ◽  
Kamal Prasad Parajuli ◽  
Kewal Shrestha

Background Urinary Tract Infection (UTI) is one of the most common infectious diseases which affect almost all ages groups of population. Production of â-lactamases is responsible for antibacterial resistance which is frequently observed in Enterobacteriaceae isolates, particularly by E. coli and Klebsiella pneumoniae. This investigation has been carried out to determine the current status of prevalence and susceptibility of uropathogens isolated among the patients at tertiary care hospital in eastern Nepal.Material and Methods This study was done at the department of Microbiology, Nobel Medical College Teaching Hospital, Biratnagar, Nepal during May 1st 2015 to October 31st 2015. Midstream cleancatch urine was sampled from 1730 suspected urinary tract infection patients of different age and sex groups. Uropathogens were recognized in term of standard and specific microbiological techniques and antimicrobial susceptibility pattern was determined by Kirby Bauer Disc diffusion method following Clinical and Laboratory Standards Institute (CLSI) guidelines.Results Out of 1730 suspected specimens Culture resulted a total of 761 (43.98 %) positive and 969 (56.02%) negative among that significant growths of uropathogens including 700 (91.98 %) unimicrobial and 60 (7.88 %) polymicrobial growths. In term of Gender distribution 443 (25.60 %) were male and 1287 (74.40 %) were female hence the ratio is 0.34:1, respectively. E. coli was the leading isolate (66 %), followed by Klebsiella spp. (12 %), Enterococcus spp. (8 %), Pseudomonas spp. (6 %), Acinetobacter anitratus (5 %), Proteus spp. (3 %).Conclusion The high frequency of multidrug resistance in bacterial uropathogens was seen. Principally, resistance patterns were seen higher for amoxycillin, co-trimoxazole, flouroquinolones and third-generation cephalosporins, Existing uropathogens highlights the highest rate of vulnerability to nitrofurantoin, amikacin and gentamicin which provide much better antibiotic coverage and can be adapted for practical treatment of urinary tract infections.  Journal of Nobel Medical College Vol.5(2) 2016; 51-55


2019 ◽  
pp. 1-2
Author(s):  
Anju K K ◽  
Deepthy B J ◽  
Gogi Suresh ◽  
Harish P V

BACKGROUND: Urinary tract infection (UTI) is the commonest bacterial infection in community practice. The most common microorganisms causing UTI include E.coli, Klebsiella, Staphylococcus aureus, Coagulase negative staphylococci, Pseudomonas, Proteus and Acinetobacter. The increase in multidrug resistance in bacterial uropathogens is an important and emerging public health problem in non-fermenting isolates.So this study focuses the surveillance of Pseudomonas aeruginosa, and Acinetobacter species in UTI and also focuses the drug resistance of the isolates. METHOD: The study was conducted at the Department of Microbiology, DM WIMS,Meppadi,Wayanad, starting from May 2019 to July 2019. A total of 200 urine samples were taken for identifying the significant urinary tract infections. Organisms were isolated and identified using standard microbial techniques. Antibiotic sensitivity was studied using Kirby Bauer disc diffusion method and EDTA double disc synergy test. RESULT: Out of the 200 urine samples studied, 87 showed significant bacteriuria, with 26 (29.9%) Pseudomonas aeruginosa and 6(6.9%) Acinetobacter species. Other isolates were E.coli (24), klebsiella (22), enterobacter (4), Citrobacter (3) and one each were Serratia and Morganella. Among these isolates 15 Pseudomonas aeruginosa and 2 Acinetobacter species were MBL producers. CONCLUSION: The study reports that other than E.coli, Pseudomonas aeruginosa has a higher prevalence in urinary tract infection and more than half of the isolates are showing drug resistance to the commonly used drugs. Most of the infection with such strains were treated successfully with combination of drugs such as Tigecycline with colistin,colistin with a carbapenem, fosfomycin with a carbapenem, fosfomycin with aminoglycoside, and a carbapenem with an aminoglycoside have been reported as antibiotic combinations effectively administered to series of patients infected with carbapenemase producing organisms.


2020 ◽  
Vol 30 (Supplement_5) ◽  
Author(s):  
S Tietz Marques ◽  
B Pitz da Silva ◽  
A Silva Mota ◽  
R Marinho de Quadros ◽  
M R de Lima

Abstract Background Urinary tract infections are common in humans. Urinalysis is the most widely used test for the diagnosis of urinary tract infection in Brazil. This study aimed to identify bacteria that cause urinary tract infection (UTI) in elderly patients permanently living in three retirement homes in the city of Lages, state of Santa Catarina, Brazil. Methods The study was approved by the Human Research Ethics Committee of University (protocol number 2.922.891). The participants were 93 elderly people, aged between 51 and 94 years. Urine samples were subjected to physical-chemical tests and to direct analysis of cell and bacterial micromorphology. Gram staining was performed on urine sediment. Positive samples were submitted to urine culture using cystine-lactose-electrolyte-deficient agar and MacConkey culture media. Bacterial colonies were identified by Gram-positive bacteria, biochemical tests and for Gram-negative bacteria, biochemical tests of oxidase and Rugai with Lysine (NewProv®). The χ2 test with the FREQ procedure of the SAS 9.2 statistical package was used, adopting p ≤ 0.05 as the level of significance, with a 95% confidence interval (CI). Results The prevalence of bacterial infection was 36.55% with rates of 46.93% in women and 25% in men. There was no positive correlation between urinary tract infections in relation to sex, age group, pH and diaper use (p &gt; 0.05). E. coli appeared in the urine of 20.43% of the elderly, S. aureus in 8,16%; S. saprophyticus in 9,09%, 5,37% with Proteus mirabilis and Klebsiella pneumoniae. Conclusions Collaborative and integrated actions are needed to facilitate the recognition and clinical management of UTI in elderly patients, which are responsibility of Federal Government social programs in partnership with states and municipalities. Key messages Elderly patients need regular monitoring. Elderly people in nursing homes need to be served by government programs.


2020 ◽  
Vol 19 (1) ◽  
pp. 133-141
Author(s):  
Vivek Kumar Singh ◽  
Mahesh Kumar Chaudhary ◽  
Megha Raj Banjara ◽  
Reshma Tuladhar

   Urinary tract infection is the most common infection in females worldwide. One in three women experiences at least one episode of urinary tract infection during their lifetime. The objective of this study was to determine the etiology and antimicrobial profile of urinary tract infection. A cross-sectional study was conducted at a tertiary care hospital in Nepal. Thirteen hundred clean catch mid-stream urine samples were tested through standard microbiological techniques. The isolates from urine samples were identified from biochemical tests. Antibiotic susceptibility testing was performed through the Kirby-Bauer disc diffusion technique following CLSI guidelines. The prevalence of urinary tract infection was found at 24.23%. Escherichia Coli was a predominant etiological agent followed by Staphylococcus aureus. The majority of the infection was found between the age group 21-40, with females mostly infected. Most of the microorganisms were isolated from emtergency, obstetrics-gynecology, and nephrology wards. Most of the isolates were resistant to ampicillin, whereas the majority of the gram-positive isolates were resistant to penicillin.A large number of isolates were found to be sensitive to Gentamycin and nitrofurantoin. Routine antimicrobial susceptibility testing of the isolates causing urinary tract infection is recommended to reduce antimicrobial resistance and for the  proper treatment of infection.


2019 ◽  
Vol 35 (6) ◽  
Author(s):  
Kaleem Ullah Zubair ◽  
Abdul Haleem Shah ◽  
Asher Fawwad ◽  
Rubina Sabir ◽  
Anum Butt

Objective: To determine the frequency of urinary tract infections and antibiotic sensitivity among patients with diabetes. Methods: This observational study was carried out in Microbiology Department of Baqai Institute of Diabetology and Endocrinology (BIDE), Baqai Medical University from April 2015 to June 2016. All patients with diabetes having symptoms of UTI attending out patients department of BIDE were analyzed. All samples received in the laboratory were processed according to Clinical and Laboratory Standards Institute (CLSI) guidelines. Antimicrobial susceptibility pattern was determined by disc diffusion method. Results: A total number of 199 urine specimens, frequency of UTI were 24 (12.06%) in male and 175 (87.94%) in female. UTIs were highly found in (age group 51-60) 70 (35.18%). Escherichia coli was the most frequent pathogen (71%), followed by Klebsiellapneumoniae (7.48%), Proteus mirabilis (1.87%), Staphylococcus aureus (9.35%), Candida (5.61%) and Candidaalbicans were (2.80%). Majority of gram negative uropathogens were shown high sensitivity towards Imipenem and Piperacillin / Tazobactam followed by Nitrofurantion, Ceftriaxone, Levofloxacin, Ofloxacine, Ciprofloxacin, Norfloxacin, Cefixime, Nalidixic acid and Cephradine. Gram positive was most sensitive to Nitrofurantionand Vancomycin followed by Piperacillin / Tazobactam, Imipenem, Cephradine, Ceftriaxone, Norfloxacin and Cefixime. Conclusion: We observed the higher frequency of UTIs in female as compared to male participants due to poor hygiene. E.coli was the most frequent pathogen responsible for UTI in patients with diabetes, followed by Staphylococcus aureus. doi: https://doi.org/10.12669/pjms.35.6.115 How to cite this:Zubair KU, Shah AH, Fawwad A, Sabir R, Butt A. Frequency of urinary tract infection and antibiotic sensitivity of uropathogens in patients with diabetes . Pak J Med Sci. 2019;35(6):1664-1668. doi: https://doi.org/10.12669/pjms.35.6.115 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


2018 ◽  
pp. 19-25

In the present study isolation and identification of pathogenic bacteria, causing urinary tract infection in infants was performed. The research work was carried out at the Microbiology Laboratory, Abasyn University, Peshawar. Samples were collected from different hospitals of District Peshawar. The collected samples were inoculated on different media for identification and isolation such as Nutrient agar, MacConkey agar, Mannitol salt agar and CLED media. For further identification Gram staining and Biochemical tests were performed such as Catalase, Oxidase, Coagulase, Indole and TSI. In total of 90 samples, E. coli was most prominent, which was found to be 52 (57.77%) followed by S. saprophyticus 22 (24.44%), K. pneumonia 11 (12.22%).While least prominent was S. aureus 5 (5.55%). Among the total isolates the percentage of Gram-negative bacteria in UTI patients was higher than Gram-positive bacteria. Whereas the percentage of E. coli was (53%) and K. pneumonia was (14%) while the percentage of S. saprophyticus was (28%) and S. aureus (5%). Among the total 90 isolates, the highest prevalence was observed in female infants about (58.88%) while in male infants, the percentage was (41.11%). Key words: Urinary Tract Infection, Infants, Pathogenic Bacteria.


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