scholarly journals Bacteriology and Antibiogram of Urinary Tract Infection Among Female Patients in a Tertiary Health Facility in South Eastern Nigeria

2017 ◽  
Vol 11 (1) ◽  
pp. 292-300 ◽  
Author(s):  
Angus N. Oli ◽  
Vivian B. Akabueze ◽  
Chijioke E. Ezeudu ◽  
George U. Eleje ◽  
Obiora S. Ejiofor ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Antibiotic Susceptibility ◽  
Empirical Treatment ◽  
Urine Samples ◽  
Diffusion Method ◽  
P Value ◽  
Female Patients ◽  
Tract Infections

Background: Urinary Tract Infection (UTI) is a common contagion among men and women with the incidence relatively higher among women due to their differing anatomy. An understanding of the kind of pathogens implicated in urinary tract infections as well as antibiotic susceptibility profiling may help the clinician make rationally correct empirical choice in their treatment. Objective: This study is aimed at determining the type and antibiotic susceptibility pattern of bacterial uropathogens isolated from female patients attending Chukwuemeka Odumegwu Ojukwu University Teaching Hospital (COOUTH), Awka, Nigeria. Method: Two hundred and forty patients with clinically diagnosed UTI and who were on at least 5 days’ antibiotic holiday were recruited into the study. Their demographic characteristics were captured using pre-tested questionnaire. Their clean catch mid-stream urine samples were collected using sterile universal container and sent to the Microbiology Department for processing. Within 30 minutes of samples collection, the specimens were cultured and the isolates were identified, after 24 h of incubation, using standard microbiological techniques. Antibiotic susceptibility tests were done with standard antibiotic discs using the Kirby–bauer disc diffusion method. Results: Out of the 240 urine samples, 89.17% yielded significant bacteriuria. The pathogens implicated were Escherichia coli (28.5%), Staphylococcus aureus (28.0%), Salmonella spp (22.8%) and Pseudomonas aeruginosa (20.5%). HIV status, patients age, pregnancy status and marital status all significantly affected bacteriuria rate (p value < 0.05), while patients’ location (sub-urban/rural dwelling), and level of education did not (p value > 0.05). The pattern of microbial resistance to antibiotics suggests that ceftazidime, fosfomycin and cefoxitin may not be used as first-line agents in the empirical treatment of UTIs rather; levofloxacin, meropenem or aztreonam should be considered. Levofloxacin was significantly effective against all the isolates and may be administered empirically while waiting for the culture result (Mean % susceptibility was 79.85). Conclusion: E. coli and S. aureus were the predominant pathogens in the study and many were resistant to the commonly prescribed antibiotics and so leave the clinicians with only few alternative drugs for UTIs treatment. Routine surveillance and monitoring studies need to be constantly conducted to update clinicians on the prevalent pathogens and the rational and empirical treatment of UTIs. Aggressive and consistent health education using every possible media is also recommended to combat the menace of drug resistance occasioned by inappropriate antibiotic use.

scholarly journals A HOSPITAL BASED SURVEILLANCE STUDY OF URINARY TRACT INFECTIONS CAUSED BY PSEUDOMONAS AERUGINOSA AND ACINETOBACTER SPECIES WITH SPECIAL EMPHASIS ON DRUG RESISTANCE

2019 ◽  
pp. 1-2
Author(s):  
Anju K K ◽  
Deepthy B J ◽  
Gogi Suresh ◽  
Harish P V
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Drug Resistance ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urinary Tract Infections ◽  
Urine Samples ◽  
Diffusion Method ◽  
Acinetobacter Species ◽  
Tract Infections

BACKGROUND: Urinary tract infection (UTI) is the commonest bacterial infection in community practice. The most common microorganisms causing UTI include E.coli, Klebsiella, Staphylococcus aureus, Coagulase negative staphylococci, Pseudomonas, Proteus and Acinetobacter. The increase in multidrug resistance in bacterial uropathogens is an important and emerging public health problem in non-fermenting isolates.So this study focuses the surveillance of Pseudomonas aeruginosa, and Acinetobacter species in UTI and also focuses the drug resistance of the isolates. METHOD: The study was conducted at the Department of Microbiology, DM WIMS,Meppadi,Wayanad, starting from May 2019 to July 2019. A total of 200 urine samples were taken for identifying the significant urinary tract infections. Organisms were isolated and identified using standard microbial techniques. Antibiotic sensitivity was studied using Kirby Bauer disc diffusion method and EDTA double disc synergy test. RESULT: Out of the 200 urine samples studied, 87 showed significant bacteriuria, with 26 (29.9%) Pseudomonas aeruginosa and 6(6.9%) Acinetobacter species. Other isolates were E.coli (24), klebsiella (22), enterobacter (4), Citrobacter (3) and one each were Serratia and Morganella. Among these isolates 15 Pseudomonas aeruginosa and 2 Acinetobacter species were MBL producers. CONCLUSION: The study reports that other than E.coli, Pseudomonas aeruginosa has a higher prevalence in urinary tract infection and more than half of the isolates are showing drug resistance to the commonly used drugs. Most of the infection with such strains were treated successfully with combination of drugs such as Tigecycline with colistin,colistin with a carbapenem, fosfomycin with a carbapenem, fosfomycin with aminoglycoside, and a carbapenem with an aminoglycoside have been reported as antibiotic combinations effectively administered to series of patients infected with carbapenemase producing organisms.

scholarly journals Bacteriological Profile and Antimicrobial Susceptibility Patterns of Urine Culture Isolates from Patients in a Tertiary Care Centre in Lalitpur

2020 ◽  
Vol 5 (1) ◽  
pp. 881-885
Author(s):  
Ruchee Manandhar ◽  
Bijendra Raj Raghubanshi ◽  
Moni Mahato ◽  
Sweekrity Neupane ◽  
Rajni Lama
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Antimicrobial Susceptibility ◽  
Pathogenic Bacteria ◽  
Tertiary Care ◽  
Urine Samples ◽  
Diffusion Method ◽  
Microbiological Methods ◽  
Tract Infections

Introduction: Urinary tract infection (UTI) is a microbial infection of the urinary system involving the urinary tract anywhere from kidney to urethra. It is one of the most common bacterial infections affecting men and women in developing countries with a high rate of morbidity and financial cost. Objectives: The objective of our study is to study the prevalence of UTI in patients attending KIST Medical College & Teaching Hospital (KISTMCTH), and determine the antimicrobial susceptibility pattern of bacteria thus isolated. Methodology: A total of 3742 urine samples from patients suspected of urinary tract infections presenting with the history and symptoms suggestive of urinary tract infection, attending various departments of KISTMCTH from April 2017 to April 2018 were studied. Isolates were identified by standard microbiological methods and tested for in vitro antibiotic susceptibility by modified Kirby-Bauer disc diffusion method. Results: Pathogenic bacteria were isolated from 646 out of 3742 urine samples (17.26%). Escherichia coli was the most common bacteria isolated (67.02%) followed by Klebsiella pneumoniae (14.5%). Other bacteria isolated were Enterococcus spp, Pseudomonas aeurigenosa, Klebsiella oxytoca, Proteus mirabilis, Satphylococcus aureus, Coagulase negative staphylococcus spp, Proteus vulgaris, Acinetobacter spp, Citrobacter freundii, Citrobacter diversus and Enterobacter spp. The isolated pathogenic bacterias were most resistant to Ampicillin (46.43%) and least resistant to Imipenem (6.03%). However, the first line antibiotic the bacterias were least resistant to was cefotaxime (7.12%). Conclusions: Marked resistance has been observed with commonly prescribed antibiotics like Ciprofloxacin and Norfloxacin. Therefore, studies should be conducted routinely to identify the common bacteria causing UTI and formulate appropriate antibiotic policy.

Antibiotic Sensitivity Pattern Among Diabetic Outpatients with Urinary Tract infection in Pontianak

2020 ◽  
Vol 14 (3) ◽  
pp. 1
Author(s):  
Mardhia Mardhia ◽  
Mahyarudin Mahyarudin ◽  
Abror Irsan
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Antibiotic Susceptibility ◽  
Antibiotic Sensitivity ◽  
Diffusion Method ◽  
Diabetic Patients ◽  
Bacterial Isolates ◽  
Sensitivity Pattern ◽  
Tract Infections

Diabetic patients are associated with a higher risk of infection. The research purposed to identify antibiotic susceptibility patterns among diabetic outpatients with urinary tract infection in Pontianak. An experimental study was performed for 13 bacterial isolates of diabetic outpatients with urinary tract infection in the Clinic of Diabetes Mellitus, Sultan Syarif Mohamad Alkadrie Hospital, Pontianak. The disc diffusion method was used to perform the susceptibility of antibiotics to the bacterial isolates. Among 13 isolates, the most common causative agent of urinary tract infection was Escherichia coli (53.85%), followed by Pseudomonas aeruginosa (30.77%). Klebsiella spp and Enterobacter aerogenes were 7.69%. Most isolates of bacteria of the study had a high sensitivity to Cefepime (92.31%), then followed by Levofloxacin, Amikacin, and Meropenem for 84.62%. The study revealed low sensitivity of bacteria to Amoxicillin/Clavulanate, Co-Trimoxazole, Cefazoline and Ceftriaxone (30.77%, 23.08 %, 23.08%, 23.08%, respectively). All bacterial isolates had high resistance to Ampicillin. Moreover, multidrug resistance observed among bacterial isolates. Keywords: antibiotic susceptibility, diabetes, urinary tract infections

scholarly journals Urinary tract infections and antibiotic susceptibility among the patients attending B & D hospital of Lalitpur, Nepal

2016 ◽  
Vol 7 (5) ◽  
pp. 47-51 ◽  
Author(s):  
Yadav Prasad Joshi ◽  
Shreejeet Shrestha ◽  
Russell Kabir ◽  
Anita Thapa ◽  
Parbati Upreti ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Tract Infection ◽  
Antibiotic Susceptibility ◽  
Bacterial Infections ◽  
Antimicrobial Agents ◽  
Cross Sectional Study ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Cross Sectional ◽  
Tract Infections

Background:Urinary tract infection is the most common bacterial infections in humans and serious health problem in many parts of the world. It has become more complicated in treatment due to different pathogens and increasing resistant to antimicrobial agents. This study aims to investigate the prevalence of urinary tract infection and antibiotic susceptibility patterns of pathogens among the patients attending in B & B hospital Nepal.Materials and Methods:A hospital based cross sectional study was conducted in between April 2010 to March 2011. Urine samples were collected from clinically suspected patients and tested bacteriologically using standard procedures. Antimicrobial susceptibility test was performed for isolated pathogen using the Kirby-Bauer disk diffusion method according to Clinical and Laboratory Standards Institute guidelines.Results:Out of 1260 examined specimens 25.24 % were positive and majority 61.64% were females.  The most common pathogens isolated were Escherichia coli (66.67%), Enterococcus (7.55%) and Staphylococcus (6.60 %). The drug resistant among the positive cases were reported. The highest resistant of positive cases was found with Cefexime (87.88%) and Enterococcus with Ampicillin (66.67%) and Staphyllococcus with Cloxacillin (66.67%). The highest susceptibility was for Vancomycin and Ampicillin i.e. 33.33% in each.Conclusion:The findings showed that E. coli isolates were the predominant pathogen and the presence of bacterial isolates with very high resistance to the commonly prescribed drugs. As drug resistance among bacterial pathogens is an evolving process and serious issue. Therefore, routine surveillance and monitoring studies should be conducted to provide physicians knowledge on the updated and most effective empirical treatment of UTIs. Asian Journal of Medical Sciences Vol.7(5) 2016 47-51

scholarly journals Microbiological profile of symptomatic and asymptomatic bacteriuria recovered from female patients with urinary tract infection

2015 ◽  
Vol 3 (1) ◽  
pp. 34-37
Author(s):  
Una Jessica Sarker ◽  
Md Sakil Munna ◽  
Saurab Kishore Munshi
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Nalidixic Acid ◽  
Asymptomatic Bacteriuria ◽  
Female Patients ◽  
Medical College ◽  
Tract Infections ◽  
Dominant Bacteria ◽  
Klebsiella Spp

With the previous knowledge on the production of ?-lactamase by the bacterial pathogens causing urinary tract infection, present study further investigated the presence of symptomatic and asymptomatic bacteriuria in female patients admitted into the Delta Medical College with suspected urinary tract infections (UTIs). The manifestation of uropathogens and their responses against locally available antibiotics (amoxicillin, 10 ?g; cephradin, 30 ?g; ciprofloxacin, 5 ?g; cotrimoxazole, 23.8 ?g; gentamicin, 10 ?g; nalidixic acid, 30 ?g) were inquired by means of conventional cultural techniques and double-disc diffusion methods, respectively. Among 110 urine samples collected from the patients with suspected UTI, 34 were found to be culture positive. Symptomatic (64.7%) and asymptomatic (36.3%) bacteriuria were noticed inside the puss cell of the UTI positive cases. Escherichia coli (73.5%) was the dominant bacteria while Klebsiella spp. (26.5%) was also exultant. Around 96% uropathogens were found to be sensitive against imipenem, and 75% against amikacin. E. coli was found to be sensitive against all of the antibiotics used, whereas Klebsiella spp. was found to be 100% resistant against nalidixic acid and cotrimoxazole. DOI: http://dx.doi.org/10.3329/sjm.v3i1.22751 Stamford Journal of Microbiology, Vol.3(1) 2013: 34-37

scholarly journals Evaluation of BacterioScan 216Dx in Comparison to Urinalysis as a Screening Tool for Diagnosis of Urinary Tract Infections in Children

2019 ◽  
Vol 57 (9) ◽  
Author(s):  
Ferdaus Hassan ◽  
Heather Bushnell ◽  
Connie Taggart ◽  
Caitlin Gibbs ◽  
Steve Hiraki ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Predictive Value ◽  
Screening Tool ◽  
Urine Culture ◽  
Antibiotic Use ◽  
Urine Samples ◽  
Maldi Tof ◽  
Tract Infections

ABSTRACTUrinalysis (UA) has routinely been used as a screening tool prior to urine culture set up. BacterioScan 216Dx is an FDA-cleared semiautomated system to detect bacterial growth in urine. The aim of this study was to evaluate 216Dx in comparison to UA for diagnosis of urinary tract infection (UTI) in children. Clean-catch, unpreserved urine samples from children aged <18 years were tested by 216Dx, and positive urine samples in media were processed for direct bacterial identification by matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry. Sensitivity and specificity of 216Dx and urinalysis (UA) were determined against urine culture. Of 287 urine samples obtained from children (median age, 108 months), 44.0% and 56.0% were UA positive and negative, respectively, while 216Dx detected 27% and 73% as positive and negative, respectively. Compared to culture, the overall sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of 216Dx versus UA were 92.1% versus 97.3%, 82.7% versus 63.8%, 44.8% versus 29.1%, and 98.6% versus 99.3%, respectively. Among 216Dx true-positive (TP) samples (n= 35), 77.0% were successfully identified directly from broth by MALDI-TOF. Among urine samples that were identified as contaminated by culture (n= 127; 44%), the 216Dx detected 93 (73.0%) as negative while UA detected 69 (54.0%) as negative. Although the sensitivities of 216Dx and UA are comparable, the specificity of 216Dx was higher than that of UA. The 216Dx can be used as an alternative/adjunct screening tool to UA to rule out urinary tract infection (UTI) in children. Compared to culture, the faster turnaround time (3 hours) of 216Dx has the potential to reduce unnecessary antibiotic use and improve patient management.

scholarly journals CHILDHOOD URINARY TRACT INFECTION: CLINICAL SIGNS, BACTERIAL CAUSES AND ANTIBIOTIC SUSCEPTIBILITY

2021 ◽  
Author(s):  
Abdulrahman A. Ishak ◽  
Abdulrahman M Alhadi ◽  
Khaled Abdulkareem A Al-Moyed ◽  
Hassan A. Al-Shamahy
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Peer Review ◽  
Urinary Tract Infections ◽  
Antibiotic Susceptibility ◽  
Clinical Symptoms ◽  
Antibiotic Sensitivity ◽  
Resistance Patterns ◽  
Tract Infections

Background and objective: Urinary tract infections (UTIs) are the neglected infection in children from the side of study its clinical symptoms, causative organisms and their antibiotic sensitivity. This investigation searches for determine clinical symptoms frequency, prevalence rate, bacterial features, and  antibiotic sensitivity of bacterial urinary tract infection in children attending private children’s health center in Sana’a city. Methods: In a prospective study carried out over a 24-month period, 1925 samples from children patients suspected of having a UTI were investigated, of which 175 were culture-positive. Clinical and demographic data were collected. Isolated bacteria were identified by standard tests, and antibiotic susceptibility was performed by the disk diffusion method. Results: Fever was the most frequent symptom that occurred (88%) while other UTI symptoms were less frequent than that reported in adult patients for UTI. The most common etiological agent was Escherichia coli (89.7%), followed Staphylococcus aureus (3.4%), Klebsiella spp (2.9%), Proteus spp (2.3%), and beta haemolytic streptococci (1.7%).  Results of antimicrobial resistant for E. coli, as the most prevalent cause of UTI, to commonly used antibiotics are ranged from less than 3% for  levofloxacin, gentamicin,  amikacin and cefoxitin to more than 75% for  tetracycline, nalidixic acid,  doxycycline,  co-trimoxazol and amoxicillin . Conclusions: The results show the most common  symptom of UTI are fever and lack of more obvious symptoms of UTI in adult patients. The antimicrobial resistance patterns of the causes of UTI are highly changeable and constant surveillance of trends in resistance patterns of uropathogens among children  is essential.                   Peer Review History: Received: 11 July 2021; Revised: 13 August; Accepted: 5 September, Available online: 15 September 2021 Academic Editor:  Ahmad Najib, Universitas Muslim Indonesia, Makassar, Indonesia, [email protected] UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency.  Received file:                Reviewer's Comments: Average Peer review marks at initial stage: 6.0/10 Average Peer review marks at publication stage: 7.0/10 Reviewers: Dr. Nuray Arı, Ankara University, Turkiye, [email protected] Dr. Salfarina Ramli,  Department of Pharmacology and Pharmaceutical Chemistry, Faculty of Pharmacy, Universiti Teknologi MARA, 42300 Puncak Alam, Selangor, Malaysia. [email protected]   Dr. Wadhah Hassan Ali Edrees, Hajja University, Yemen, [email protected] Dr. Asia Selman Abdullah, University of Basrah, Iraq, [email protected] Similar Articles: URINARY TRACT INFECTIONS IN POST OPERATIVE PATIENTS: PREVALENCE RATE, BACTERIAL PROFILE, ANTIBIOTIC SENSITIVITY AND SPECIFIC RISK FACTORS  BIOFILM FORMATION AND ANTIBIOTIC SUSCEPTIBILITY OF UROPATHOGENS IN PATIENTS WITH CATHETER ASSOCIATED URINARY TRACT INFECTIONS IN IBB CITY -YEMEN

scholarly journals Prevalence of blaCTX-M, blaSHV, and blaTEM Genes in Escherichia coli Strains Isolated From Urinary Tract Infection Samples of Patients in the Intensive Care Unit in Qom, Iran

2020 ◽  
Vol 7 (2) ◽  
Author(s):  
Shima Sadat Lesani ◽  
Mohammad Soleimani ◽  
Pegah Shakib ◽  
Mohammad Reza Zolfaghari
Keyword(s):  
Escherichia Coli ◽  
Drug Resistance ◽  
Urinary Tract Infection ◽  
Intensive Care ◽  
Urinary Tract ◽  
Tract Infection ◽  
Intensive Care Units ◽  
Diffusion Method ◽  
E Coli ◽  
Tract Infections

Background: Escherichia coli is considered as one of the causes of opportunistic infections. Nowadays, due to the increase in drug resistance, the treatment of these infections has become very difficult and they are recognized as the main causes of death in hospitalized patients. Objectives: The aim of this study was to determine the prevalence of blaTEM, blaSHV, and blaCTX-M genes in E. coli strains isolated from the urinary tract infection in patients in Intensive Care Units of three different hospitals in Qom, Iran. Methods: This study was conducted in three months from October to December 2014. A total of 200 E. coli samples were taken from the patients with urinary tract infections in Intensive Care units of Qom hospital. The disc diffusion method was used to determine the susceptibility pattern of antibiotic and phenotypic confirmatory tests for screening of the expanded spectrum beta-lactamase (ESBL) isolates. The presence of blaTEM, blaSHV, and blaCTX-M genes was evaluated by the polymerase chain reaction (PCR) assay. Results: Of 200 samples, ampicillin (96%) and nitrofurantoin (19.5%) showed the highest and lowest drug resistance, respectively. A total of 156 isolates (78%) were identified as ESBLs using the phenotypic method. Moreover, 76 (38%), 90 (45%), and 123 (61.5%) isolates consisted of blaCTX-M, blaSHV, and blaTEM, respectively. Conclusions: Overall, the findings of this study showed that blaTEM was the most common gene with a frequency of 61.5% in ESBL E. coli.

scholarly journals Antimicrobial Efficacy of Chromolaena odorata Extract against Bacteria Isolated from Patients with Urinary Tract Infection in Ondo State, Nigeria

2018 ◽  
pp. 1-10
Author(s):  
Anthony Okhonlaye Ojokoh ◽  
Olumide Mogaji ◽  
Bartholomew Saanu Adeleke
Keyword(s):  
Antimicrobial Activity ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urine Samples ◽  
Antimicrobial Efficacy ◽  
Chromolaena Odorata ◽  
Candida Sp ◽  
Ondo State ◽  
Tract Infections

Aim: This study is designed to isolate and identify the microorganisms associated with urinary tract infection in infected patients, determine the antimicrobial resistance pattern on the microbial isolates and the antimicrobial efficacy of solvents leaf extract of Chromolaena odorata on the resistant isolates. Methodology: Six hundred (600) mid-stream urine samples were collected from infected patients in some selected hospitals in Ondo State. The phytochemical screening of the plant was determined. The antibiotics sensitivity and antimicrobial activity of the plant extract was determined using plate assay and agar well diffusion methods. Results: Out of 600 urine samples analyzed, 472 (78.68%) showed significant growth of uropathogens comprising 197 (32.80%) male and 275 (45.83%) female. The isolated microorganisms comprise 5 Gram-positive bacteria (GPB), 9 Gram-negative bacteria (GNB) and 4 Candida sp. GNB account for 284(58.79%), GPB 158(32.71%) and Candida sp 41(8.48%). Escherichia coli showed high prevalence 90(18.63%) while Klebsiella ozaenae showed the least 11(2.27%). The phytochemical constituents of C. odorata extracts contained tannins, flavonoids, saponins, steroids and terpernoids and bacterial isolates showed resistant to all the antibiotics. Pseudomonas mendocina and P. putida showed the least resistant to C. odorata extracts. The MIC of the leaf extracts ranged from 5.0 mg/ml to 50.0 mg/ml.  The methanol extract had the highest antimicrobial activity than n-hexane and chloroform extracts on the uropathogens. Conclusion: The high therapeutic potentials of C. odorata extract against the uropathogens could be used as alternative phytotherapy in herbal medicine to the commercial antibiotics in the treatment of urinary tract infections.

scholarly journals Investigation of bacteria liable for Urinary Tract Infections and their antibiogram analysis

2018 ◽  
pp. 26-35
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
High Rate ◽  
Diffusion Method ◽  
High Resistivity ◽  
Protein Synthesis Inhibitor ◽  
Glycopeptide Antibiotics ◽  
Biochemical Tests ◽  
Tract Infections

Urinary tract infection mainly occurs by microorganism when they overcome the human immune system. These microbes have caused the high rate of morbidity and motility effect all over the world to every age and gender. Lower urinary tract infection is more common among females than males. A prospective observational study was conducted in Pathology Department at Lady Reading Hospital Medical Teaching Institution, Peshawar, Pakistan in a 4th quarter (October, November, December) of the year 2017. Mid-stream urine samples were collected from 1357 patients from all hospital-admitted and outdoor patients. They were cultured on CLED agar, after isolation, uropathogens was identified through biochemical tests like oxidase, catalase and TSI tests. Only 205 samples were reported positive, with the prevalence of 15.10%. Females (51.70%) were more prone to UTI then males (48.29%). E.coli was most frequently isolated uropathogen with frequency of 90.24%. Other isolates were Pseudomonas aeruginosa (4.8%), Enterococcus faecium (2.92%), Enterobacter species (1.46%) and Proteus mirabilis (0.49%). Thirteen antibiotics were used to test the susceptibility of isolated microbes through Kirby Bauer Disc Diffusion method. Carbapenem drug like Imipenem (89.7%) was most effective against gram-negative microbes belonging to Enterobacteriaceae family. Other antibiotics like, Piperacillin + Tazobactam (84.86%), Cefoperazone /Sulbactam (84.3%), Amikacin (84.3%), Fosfomycin (83.7%) and Nitrofurantoin (77.2%) also showed sensitivity. Fluoroquinolones Drugs like Ciprofloxacin (82.1%) and Levofloxacin (81.6%) showed high resistivity followed by Co-trimoxazole (80.54%) and Cephalosporine drugs. For Gram positive Enterococcus spp, Glycopeptide antibiotics and protein synthesis inhibitor drugs had shown 100% sensitivity, but carbapenem and fluoroquinolones were highly resistant. Keywords: Enterobacteriaceae Family, Urinary Tract Infection (UTI), Fluoroquinolones Drug

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