scholarly journals PENGARUH PENGGUNAAN KONSENTRASI AIR KELAPA MUDA PADA PENGENCER NACL FISIOLOGIS TERHADAP MOTILITAS DAN MORTALITAS SPERMATOZOA IKAN TAWES ( Puntius javanicus)

2015 ◽  
Vol 2 (2) ◽  
Author(s):  
Farah Diana ◽  
Muhammad Rizal ◽  
Dewi Mariani
Keyword(s):  
Coconut Water ◽  
Motility Index ◽  
Sample Quality ◽  
Randomized Design ◽  
Research Show

This study aims to knowing influence using cocentration of coconut water in physiology of NaCl dilution toquality of spermatozoa of Puntius javanicus. This research was counducted at Meunasah Krueng Village, Beutong Subdistrict, Nagan Raya District from 18th – 20th May 2015. Thecompletely randomized design with 5 treatmentand 3 replication was used for this study. Sample was saved in refrigerator (3 days) with temperature 4oC and it is oberved sample quality and data was collected in daily. Result of the research show water coconut concentration (7%) or in treatment 4th (P4) giving result for best quality of spermatozoa, where motility index average of spermatozoa is up to 80,55%, viability value of spermatozoa is up to 65,88%. In addtion, coconut water in in physiology of NaCl dilution have been affecting to spermatozoa quality of Puntius javanicus. 

scholarly journals PENGARUH PENGGUNAAN KONSENTRASI AIR KELAPA MUDA PADA PENGENCER NACL FISIOLOGIS TERHADAP KUALITAS SPERMATOZOA IKAN TAWES ( Puntius javanicus)

2015 ◽  
Vol 2 (2) ◽  
Author(s):  
Muhammad Rizal ◽  
Farah Diana ◽  
Dewi Mariani
Keyword(s):  
Coconut Water ◽  
Replication Sample ◽  
Sample Quality ◽  
Randomized Design ◽  
Mortality Index ◽  
Completely Randomized Design

This study aims to knowing influence using cocentration of coconut water in physiology of NaCl dilution to spermatozoa quality ofPuntius javanicus. This research was counducted at Meunasah Krueng Village, Beutong Subdistrict, Nagan Raya District from 18th – 20th May 2015. This study was using completely randomized design with 5 treatmentand 3 replication. Sample was saved in refrigerator (3 days) with temperature 4oC and it is oberved sample quality and data was collected in daily. Result of the study show water coconut concentration (7%) giving result for best quality of spermatozoa, where mortality index average of spermatozoa is up to 80,55%, viability value of spermatozoa is up to 65,88% and spermatozoa mortality is up to 23,88%. In addtion, coconut water in in physiology of NaCl dilution have been affecting to spermatozoa quality of Puntius javanicus.

Motilitas dan Vabilitas Spermatozoa Ayam Kampung pada Suhu 5°C Menggunakan Pengencer dan Lama Simpan yang Berbeda

2018 ◽  
Vol 5 (2) ◽  
pp. 102
Author(s):  
Enike Dwi Kusumawati ◽  
Selvinus Lawu Woli ◽  
Aju Tjatur Nugroho Krisnaningsih ◽  
Waluyo Edi Susanto ◽  
Syam Rahadi
Keyword(s):  
Data Analysis ◽  
Storage Temperature ◽  
Coconut Water ◽  
Laboratory Research ◽  
Ringer Lactate ◽  
Randomized Design ◽  
Native Chicken ◽  
Lactate Solution ◽  
Better Than

ABSTRAKPenelitian ini dilakukan untuk mengetahui motilitas dan viabilitas spermatozoa ayam kampung pada suhu 5oC menggunakan pengencer dan lama simpan yang berbeda. Metode yang digunakan dalam penelitian ini adalah penelitian laboratorium menggunakan Rancangan Acak Lengkap (RAL) Faktorial dengan pengencer ringer lactat solution, air kelapa dan tanpa pengencer serta lama simpan 0, 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, masing-masing diulang 10 kali. Variabel yang diamati yaitu motilitas dan viabilitas spermatozoa. Analisa data yang digunakan adalah analisis varian. Hasil analisis data menunjukkan bahwa motilitas dan viabilitas spermatozoa menggunakan pengencer ringer lactat solution lebih tinggi (P<0,01) serta dapat bertahan sampai lama simpan 24 jam dibandingkan air kelapa dan tanpa pengencer. Adapun nilai motilitas ringer lactat solution, air kelapa dan tanpa pengencer pada lama simpan 24 jam masing-masing sebesar 43,5±17,17%; 8±4,83%; 6,5±2,4%, sedangkan nilai viabilitasnya sebesar 83,2±7,25%; 64,6±3,20%; dan 63,1±2,33%. Kesimpulan dari hasil penelitian ini adalah ringer lactat solution lebih baik dibandingkan air kelapa dan tanpa pengencer dalam mempertahankan kualitas semen ayam kampung pada suhu simpan 5oC sampai lama simpan 24 jam.Kata Kunci : air kelapa, ayam kampung, motilitas, spermatozoa, viabilitas  ABSTRACTThis study was conducted to determine the motility and viability of spermatozoa of Native chickens at 5oC using different diluents and time storage. The method used in this study was laboratory research using Factorial Completely Randomized Design with ringer lactate solution, coconut water and without diluent at 0, 3, 6, 9, 12, 15, 18, 21, 24, 27, 30 hours of time storage each repeated 10 times. The variables observed were motility and viability of sperm. Data analysis used is variance analysis. The results of data analysis showed that the motility and viability of spermatozoa using ringer lactate solution diluents was higher (P <0.05) than coconut water and without diluents. The motility values of ringer lactat solution, coconut water and without diluents were 43,5±17,17%; 8±4,83%; 6,5±2,4% respectively, while the viability values were 83,2±7,25%; 64,6±3,20% and 63,1±2,33%. The conclusion of this study is that ringer lactat solution is better than coconut water an without diluents in maintaining the quality of Native chicken semen at a storage temperature of 5oC until 24 hours.Keywords: coconut water, motility, native chicken, sperm, viability

scholarly journals THE ADDITION OF COCONUT WATER AND THIAMINE TOWARDS CHRYSSANTHENUM MICRO CUTTINGS (Dendranthema grandiflora Tzvelev) IN VITRO

Agrivet ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 39
Author(s):  
Salma Nabila ◽  
Endah Budi Irawati ◽  
Rina Srilestari
Keyword(s):  
Water Concentration ◽  
Coconut Water ◽  
Fresh Weight ◽  
Randomized Design ◽  
Two Factors ◽  
Completely Randomized Design ◽  
Range Test ◽  
Growing Shoot

Chryssanthenum is ornamental plant with variety of shape and color which are unique and appealing. So that, it is in great demand in the community. The production of Chrissanthenum conventionally hampered by availability and quality of seeds. Thus, it needs research through tissue culture. The aim of this research is to know interaction between coconut water and thiamine and to determine the best coconut water and thiamine concentration toward Chryssanthenum micro cuttings. This research used laboratory experimental method by using completely randomized design with two factor. The 1st factor was coconut water concentration consisted of three level which were 5%, 10% and 15%. The 2nd factor was thiamine concentration consisted of three level which were 1mg/L, 2 mg/L and 3 mg/L. From the two factors, those were found that, there were nine combination of treatments and repeated 3 times. The variety  of data was analyzed by using Analysis of Variance (ANOVA) with level of α=5%, and continued by examining Duncan’s Multiple Range Test (DMRT) with level of α=5%. The result indicated that the interaction of coconut water concentration was 5% and thiamine was 1 mg/L on the parameters when growing shoot. There was also interaction on coconut water concentration which was 10 % and thiamine was 1 mg/L on the parameters in the number of shoots. interaction of coconut water combination was 15% and thiamine was 2 mg/L on  fresh weight. The addition of 10% coconut water and 1 mg/L thiamine showed the best result on shoot length.

scholarly journals Percepatan Penyediaan Benih Sumber Kedelai Unggul Secara In Vitro

Agrikultura ◽  
2017 ◽  
Vol 28 (3) ◽  
Author(s):  
Erni Suminar ◽  
Sumadi Sumadi ◽  
Syariful Mubarok ◽  
Toto Sunarto ◽  
Nita Suswati Endah Rini
Keyword(s):  
Growth Failure ◽  
Culture Method ◽  
Coconut Water ◽  
High Quality ◽  
Seed Technology ◽  
Randomized Design ◽  
Vitamin B5 ◽  
New Varieties

ABSTRACTIn vitro method to fasten availability high quality soybean seedsSoybean is an important crop as a source of food and its demand has increased every year. Several new varieties of soybean have been generated, but the number is still limited. Furthermore, the infestation of pests and infection of diseases have also limited the new soybean varieties production as it increase the risk of growth failure. Therefore, methods to fasten the availability of high quality of soybean seeds need to be developed. One of which can be done through in vitro culture method. The objective of this study was to obtain the best type and the best concentration of cytokinin for the growth of soybean explants in vitro. The experiment was conducted in Seed Technology and Tissue Technology Laboratory, Faculty of Agriculture, Universitas Padjadjaran. The experimental design used was Completely Randomized Design with 13 treatments and four replications. The basic media used were Murashige and Skoog (MS) + Vitamin B5 with addition of BAP (1.0 mg/l, 1.5 mg/l, and 2.0 mg/l), Kinetin (0.5 mg/l, 1.0 mg/l, and 1.5 mg/l), TDZ (0.01 mg/l, 0.1 mg/l, and 1.0 mg/l), and coconut water (10%, 15%, and 20%). The result showed that cytokinin types and concentrations gave different effect to soybean explant growth. The best treatment was demonstrated by BAP at the concentration of 1.5 mg/l as shown by the highest percentage of leaves and the number of shoots. However, it did not affect the percentage of buds produced by explant and the percentage of callus produced by explant.Keyword : BAP, Kinetin, TDZ, Coconut water, Soybean.AbstrakKedelai merupakan komoditas yang memegang peranan penting. Permintaan akan kedelai meningkat setiap tahunnya. Varietas kedelai baru yang bersifat unggul sudah banyak dihasilkan, namun jumlahnya masih terbatas. Tingginya serangan hama dan penyakit saat perbanyakkan benih di lapangan menyebabkan tingginya resiko kegagalan dalam pertumbuhan varietas baru tersebut. Sehingga perlu dilakukan percepatan penyediaan benih sumber varietas unggul di laboratorium secara in vitro. Tujuan penelitian ini adalah untuk mendapatkan jenis dan konsentrasi sitokinin yang terbaik untuk pertumbuhan eksplan kedelai in vitro. Penelitian dilakukan di Laboratorium Kultur Jaringan Teknologi Benih Fakultas Pertanian Universitas Padjadjaran. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap dengan tiga belas perlakuan dan empat ulangan. Media dasar yang digunakan adalah Murashige dan Skoog (MS) + Vitamin B5 dengan penambahan BAP (1,0 mg/l; 1,5 mg/l; 2,0 mg/l), Kinetin (0,5 mg/l; 1,0 mg/l; 1,5 mg/l), TDZ (0,01 mg/l; 0,1 mg/l; 1,0 mg/l), dan air kelapa (10%; 15%; 20%). Hasil percobaan menunjukkan bahwa pemberian jenis dan konsentrasi sitokinin memberikan pengaruh yang berbeda terhadap pertumbuhan eksplan. Perlakuan terbaik diperoleh pada BAP dengankonsentrasi 1,5 mg/l yang ditunjukkan dengan tingginya persentase jumlah daun dan tunas yang terbentuk. Akan tetapi, penambahan sitokinin tersebut tidak memberikan pengaruh terhadap persentase eksplan dalam membentuk tunas dan persentase eksplan dalam membentuk kalus.Kata Kunci : BAP, Kinetin, TDZ, Air Kelapa, Kedelai

scholarly journals Daya Hidup Spermatozoa Epididimis Sapi Persilangan yang Dipreservasi dengan Air Kelapa Muda pada Suhu 5oC (VIABILITY OF EPIDIDYMAL SPERMATOZOA CROSSBREED CATTLE PRESERVED WITH COCONUT WATER AT 5oC)

2018 ◽  
Vol 18 (4) ◽  
pp. 571
Author(s):  
Muhammad Rizal ◽  
Muhammad Riyadhi ◽  
Bambang Irawan ◽  
Anis Wahdi ◽  
Habibah Habibah ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Saline Solution ◽  
Egg Yolk ◽  
Coconut Water ◽  
Randomized Design ◽  
Significant Difference ◽  
Epididymal Spermatozoa ◽  
Completely Randomized Design ◽  
Difference Test

The objective of this research was to examine the effectiveness of coconut water with various egg yolk concentrations in maintaining the viability of epididymal spermatozoa of crossbreed cattle preserved at 5oC. Five testis with epididymides of crossbreed cattle were obtained from slaughterhouse. Epididymal spermatozoa was collected by the combination of slicing, flushing and tissues pressure methods of cauda epididymides with saline solution (0.9% NaCl). Collected-spermatozoa was equally divided in volume into four tubes and diluted with lactose extender containing 20% egg yolk (control), 90% coconut water + 10% egg yolk (CWEY10), 85% coconut water + 15% egg yolk (CWEY15), and 80% coconut water + 20% egg yolk (CWEY20), repectively. Diluted-spermatozoa was stored in refrigerator at 5oC. Quality of dilutedspermatozoa including percentages of motile spermatozoa (MS), live spermatozoa (LS), spermatozoa with intact plasma membrane (IPM) were evaluated every day during four days of storage. Data were analyzed by using completely randomized design with four treatments and five replicates. Means were compared with significant difference test at 0.05 significant level. Results of this study showed that mean of spermatozoa concentration, percentage of MS, percentage of LS, percentage of spermatozoa abnormal, and percentage of IPM of crossbreed cattle fresh epididymal spermatozoa were 1,414 million cell/ml, 72%, 85%, 9%, and 90%, respectively. At day-4 of the storage, percentages of MS, LS, and IPM of control (43, 52.2, 59.2%) and CWEY20 (42, 52, 59%) were significantly (P<0.05) higher than CWEY10 (33, 45.4, 52.8%) and CWEY15 (37, 50, 54.6%). In conclusion, lactose and CWEY20 extenders could be maintaining the quality of epidydimal spermatozoa of crossbreed cattle for three days preservation at 5oC and two days for CWEY10 and CWEY15.

scholarly journals Pengaruh Komposisi Kuning Telur pada Pengencer Air Kelapa Hijau Terhadap Kualitas Semen Cair Kambing Boer

2020 ◽  
Vol 7 (2) ◽  
pp. 152
Author(s):  
Nisa'us Sholikah ◽  
Sri Susilowati
Keyword(s):  
Sperm Quality ◽  
Block Design ◽  
Animal Husbandry ◽  
Coconut Water ◽  
Boer Goat ◽  
Animal Reproduction ◽  
Sperm Abnormality ◽  
Semen Extender ◽  
Research Show

ABSTRAKAir kelapa dapat digunakan sebagai bahan alternatif pengencer semen cair karena mengandung unsur karbon yang dibutuhkan untuk menjaga kualitas spermatozoa. Penelitian ini bertujuan untuk mengetahui pengaruh perbedaan komposisi kuning telur pengencer air kelapa hijau terhadap kualitas semen cair kambing boer. Penelitian dilaksanakan di Laboratorium Reproduksi Ternak Fakultas Peternakan Universitas Brawijaya, Malang, pada bulan November 2019. Penelitian ini menggunakan metode eksperimental laboratorium dengan rancangan acak kelompok (RAK). Data dianalisis menggunakan analisis ragam (ANOVA). Terdapat 4 perlakukan, P0 = CEP-3 + 10% kuning telur, P1 = Pengencer air kelapa + 5% kuning telur, P2 = Pengencer air kelapa + 10% kuning telur, dan P3 = Pengencer air kelapa + 15% kuning telur. Hasil menunjukkan bahwa terdapat perbedaan yang signifikan (P<0,01) pada motilitas individu dan viabilitas spermatozoa, akan tetapi tidak berpengaruh signifikan (P>0,05) pada abnormalitas spermatozoa antara perlakuan selama peyimpanan dingin. Kesimpulan penelitian adalah perlakuan terbaik didapatkan pada P3 yang dapat mempertahankan kualitas semen cair kambing boer sampai penyimpanan hari ke-3.Kata kunci: air kelapa hijau, kuning telur, kambing boer, semenABSTRACTCoconut water extender can be used as an alternative semen extender because it contains the carbon element needed to maintain the quality of spermatozoa. The aims of this research were to examine the effect of different compositions of yolk in green coconut water extender on the quality of boer goat semen during cold storage. This research was carried out at the Laboratory of Animal Reproduction of Animal Husbandry Faculty of Brawijaya University in November 2019. The research used a laboratory experimental method. The experimental design was a randomized complete block design. The data were analyzed by Analyze of Variance. In this research there were four treatments (P0 = CEP-3 + 10% yolk; P1 = Coconut Water + 5% yolk; P2 = Coconut Water + 10% yolk; dan P3 = Coconut Water + 15% yolk). The result of this research show were significant differences (P<0.01) in sperm motility and viability, but no significant differences (P>0.05) in sperm abnormality between coconut water extenders with different compositions of yolk. In conclusion, the best treatment was P3 in maintaining boer goat sperm quality after three days of chilled preservation.Keywords: boer goat, green coconut water, semen, yolk

scholarly journals Pengaruh Air Kelapa Terhadap Kualitas Fisik Daging Sapi

2019 ◽  
Vol 7 (2) ◽  
Author(s):  
D Wahyuni ◽  
F Yosi ◽  
G Muslim
Keyword(s):  
Ph Value ◽  
Water Holding Capacity ◽  
Coconut Water ◽  
Physical Quality ◽  
Randomized Design ◽  
Value Range ◽  
Completely Randomized Design ◽  
Water Holding

Tujuan dari penelitian ini adalah untuk mengetahui pengaruh air kelapa sebagai bahan rendaman daging terhadap kualitas fisiknya. Penelitian menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 4 perlakuan. Perlakuan terdiri dari kontrol (P0),  perendaman daging sapi kedalam air kelapa selama 30 menit (P1), perendaman selama 60 menit (P2), dan perendaman selama 90 menit (P3). Peubah yang diamati dalam penelitian ini adalah pH, daya ikat air dan keempukan daging sapi. Hasil penelitian menunjukan bahwa pH, daya ikat air dan keempukan daging sapi yang diberi perlakuan rendaman air kelapa tidak berbeda nyata dengan kontrol. Nilai pH daging berada di kisaran 5,74-5,86, daya ikat air 33,62-46,21% dan keempukan 6,90-9,39 N. Kesimpulannya adalah penggunaan air kelapa sebagai bahan rendaman daging sapi belum mampu mempengaruhi pH, daya ikat dan keempukan. Kata kunci: Air Kelapa, Daging Sapi, Kualitas Fisik The aim of this research was to find out the effect of coconut water to physical quality of beef. The research designed by Completely Randomized Design (CRD) which consisted 4 treatments. The treatments consisted were control (P0), soaked beef in coconut water for 30 minutes (P1), soaked for 60 minutes (P2) and soaked for 90 minutes (P3). The parameter observed in this research were pH, water holding capacity and tenderness. The result of this research showed that pH, water holding capacity and tenderness of soaked beef had not significantly different than control. Beef pH value range were 5.74-5.86, water holding capacity 33.62-46.21% and tenderness 6.90-9.39 N. The conclusion was the usage of coconut water as beef soaking material didn’t affect pH, water holding capacity and tenderness.  Keyword: Coconut Water, Beef, Physical Quality.

THE USE OF COCONUT WATER FOR RAW MATERIAL OF VINEGAR

2012 ◽  
Vol 2 (2) ◽  
pp. 53-61
Author(s):  
Hasanuddin Hasanuddin ◽  
Kurnia Herlina Dewi ◽  
Okta Wulandra
Keyword(s):  
Fruit Juice ◽  
Coconut Water ◽  
Raw Material ◽  
Aerobic Fermentation ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Two Stages ◽  
Water Waste ◽  
Range Test

Vinegar could be produced from any fruit juice. Coconut water as raw material was used by increasing sugar concentration. Vinegar is one of the alternatives in the use of coconut water waste. This is supported by the needs of the growing vinegar. Vinegar  manufacture involves two stages of fermentation (anaerobic and aerobic). Aerobic fermentation by adding yeast and sugar yield of 12% alcohol (the alcohol optimal), where as aerobic fermentation produces vinegar 4% -12.5% (SNI). The purpose of this study were to determine the optimal percentage of the addition of yeast and sugar to produce alcohol 12%, and to compare the quality of coco vinegar with SNI 01-3711-1995 vinegar. This study used factorial completely randomized design , the adding sugar and  yeast as treatment, 3 level of adding sugar and  yeast with 3 observations. Results of variance analysis  showed that the treatment was very real effect on levels of alcohol and alcohol pH. The Duncans Multiple Range Test (DMRT) showed that the highest quality levels of alcohol present in addition of 16% sugar and   6% of yeast. while the pH of alcohol contained in the addition of yeast 4.5% and sugar 10%

scholarly journals The Effect of the Combination of Glucose Concentration with the Type of Extenders on the Quality of Native Rooster Spermatozoa during Storage

2020 ◽  
Vol 4 (2) ◽  
pp. 36-43
Author(s):  
K. Khaeruddin ◽  
Muhammad Amir
Keyword(s):  
Glucose Concentration ◽  
Skim Milk ◽  
Egg Yolk ◽  
Coconut Water ◽  
Progressive Motility ◽  
Randomized Design ◽  
Completely Randomized Design

This study aimed to findthe best combination of glucose concentration with the type of extender to maintain progressive motility and viability of Native Rooster spermatozoa, which stored for 48 hours at 5oC. This study used a completely randomized design (CRD) factorial pattern of 4 x 3. The first treatment was a different type of extender: LE (lactated ringer egg yolk), LS (lactated ringer skim milk), CE (coconut water egg yolk) and CS (coconut water skim milk), while second treatment was glucose concentration (20mM, 50mM, and 80mM).  Native Rooster semen diluted with a ratio of 1:10, thensperm was stored in the refrigerator (5oC). Progressive motility and viability were observed at 0, 24, 48, and 72 hours. The results showed the type of extender treatment had a very significant effect (P <0.01) on the viability of spermatozoa, which were stored 24-hour. The treatment of extender type, glucose concentration, and interaction of extender type with glucose concentration had a very significant effect (P <0.01) on the quality of spermatozoa, which were stored 48 hours and 72 hours. The highest progressive motility of spermatozoa was maintained up to 46.67 ± 2.89% and spermatozoa viability up to 74.68 ± 4.51% in WE 80mM glucose extenders, so the conclusion of this study is the combination of egg yolk coconut water with 80mM glucose is the best extender to maintain progressive motility and viability of spermatozoa of Native Rooster which is stored for 72 hours at 5oC.

The Utilization of Red Dragon Fruit Peel Extract (Hylocereus costaricencis) As A Natural Colorant of Dried Noodle

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Silaturahmi Silaturahmi ◽  
Zaidiyah Zaidiyah ◽  
Tengku Mia Rahmiati
Keyword(s):  
Product Diversification ◽  
Fruit Peel ◽  
Physical Quality ◽  
Natural Colorant ◽  
Randomized Design ◽  
Solid Loss ◽  
Dragon Fruit ◽  
Completely Randomized Design ◽  
Peel Extract

The use of colorants in the dried noodle manufactures is an effort of product diversification. Besides of being used as a natural coloring agent, this peel extract is also used to improve nutritional value of the expected product. The purpose of this study was to determine the effect of red dragon fruit peel extract on the physical quality of dried noodle.  The study was conducted by using Completely Randomized Design (CRD) method with one factor, namely concentration of red dragon fruit peel extract (N1 = 10 ml, N2 = 15 ml, N3 = 20 ml, N4 = 25 ml, N5 = 30 ml).  The observations of its physical quality consisted of water absorption, solid loss during cooking, and organoleptic tests (aroma and color).  The best quality of dried noodle was obtained by using 30 ml red dragon fruit peel extract (N5) with physical properties, namely DSA levels of 351.92%, solid loss during cooking 4.78%, aroma 3.79 (like) and color 3.89 (like).

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