scholarly journals Pengaruh Komposisi Kuning Telur pada Pengencer Air Kelapa Hijau Terhadap Kualitas Semen Cair Kambing Boer

2020 ◽  
Vol 7 (2) ◽  
pp. 152
Author(s):  
Nisa'us Sholikah ◽  
Sri Susilowati
Keyword(s):  
Sperm Quality ◽  
Block Design ◽  
Animal Husbandry ◽  
Coconut Water ◽  
Boer Goat ◽  
Animal Reproduction ◽  
Sperm Abnormality ◽  
Semen Extender ◽  
Research Show

ABSTRAKAir kelapa dapat digunakan sebagai bahan alternatif pengencer semen cair karena mengandung unsur karbon yang dibutuhkan untuk menjaga kualitas spermatozoa. Penelitian ini bertujuan untuk mengetahui pengaruh perbedaan komposisi kuning telur pengencer air kelapa hijau terhadap kualitas semen cair kambing boer. Penelitian dilaksanakan di Laboratorium Reproduksi Ternak Fakultas Peternakan Universitas Brawijaya, Malang, pada bulan November 2019. Penelitian ini menggunakan metode eksperimental laboratorium dengan rancangan acak kelompok (RAK). Data dianalisis menggunakan analisis ragam (ANOVA). Terdapat 4 perlakukan, P0 = CEP-3 + 10% kuning telur, P1 = Pengencer air kelapa + 5% kuning telur, P2 = Pengencer air kelapa + 10% kuning telur, dan P3 = Pengencer air kelapa + 15% kuning telur. Hasil menunjukkan bahwa terdapat perbedaan yang signifikan (P<0,01) pada motilitas individu dan viabilitas spermatozoa, akan tetapi tidak berpengaruh signifikan (P>0,05) pada abnormalitas spermatozoa antara perlakuan selama peyimpanan dingin. Kesimpulan penelitian adalah perlakuan terbaik didapatkan pada P3 yang dapat mempertahankan kualitas semen cair kambing boer sampai penyimpanan hari ke-3.Kata kunci: air kelapa hijau, kuning telur, kambing boer, semenABSTRACTCoconut water extender can be used as an alternative semen extender because it contains the carbon element needed to maintain the quality of spermatozoa. The aims of this research were to examine the effect of different compositions of yolk in green coconut water extender on the quality of boer goat semen during cold storage. This research was carried out at the Laboratory of Animal Reproduction of Animal Husbandry Faculty of Brawijaya University in November 2019. The research used a laboratory experimental method. The experimental design was a randomized complete block design. The data were analyzed by Analyze of Variance. In this research there were four treatments (P0 = CEP-3 + 10% yolk; P1 = Coconut Water + 5% yolk; P2 = Coconut Water + 10% yolk; dan P3 = Coconut Water + 15% yolk). The result of this research show were significant differences (P<0.01) in sperm motility and viability, but no significant differences (P>0.05) in sperm abnormality between coconut water extenders with different compositions of yolk. In conclusion, the best treatment was P3 in maintaining boer goat sperm quality after three days of chilled preservation.Keywords: boer goat, green coconut water, semen, yolk

Kualitas Semen Cair Kambing Boer Berbahan Pengencer Air Kelapa Muda Varietas Viridis Setelah Simpan Dingin

2019 ◽  
Vol 6 (1) ◽  
pp. 78
Author(s):  
Muhammad Ade Salim ◽  
Muhammad Nur Ihsan ◽  
Nur Isnaini ◽  
Trinil Susilawati
Keyword(s):  
Semen Quality ◽  
Block Design ◽  
Animal Husbandry ◽  
Egg Yolk ◽  
Coconut Water ◽  
Boer Goat ◽  
Randomized Block Design ◽  
Frozen Semen ◽  
Artificial Vagina

ABSTRAKAir kelapa muda varietas viridisdapat dijadikan pengencer aletrnatif semen cair bagi program IB di daerah minim sarana semen beku. Tujuan penelitian ini untuk menguji pengaruh penggunaan air kelapa muda viridissebagai bahan pengencer terhadap kualitas semen cair kambing Boer setelah didinginkan. Dilaksanakanselama 3 bulan di Laboratorium Fakultas Peternakan UBUnit SumberSekar,Malang. Metodenya yaitu eksperimen. Semen dari  3 pejantan Boer umur 3-5 tahun, dikoleksi seminggu sekali dengan VB. Air kelapa mudaviridis umur 5-7 bulan serta tris aminomethane sebagai kontrol. Didesain menggunakan Rancangan Acak Kelompok (RAK) dengan 2 perlakuan yaitu P0 (tris aminomethane + 10% KT) dan  P1 (air kelapa muda viridis + 10% KT) masing-masing diulang 10 kali. Data dianalisis dengan analisis Ragam (Anova) dengan software Genstat 18. Variabelnya yaitu motilitas individu, viabilitas dan abnormalitas. Hasil penelitian yaitu motilitas individu pada P1bertahan sampai 4 hari (40,5± 24,3%), viabilitas terbaik sampai hari ke-5 (42±24,6%), abnormalitas terendah di hari ke-7(1,31± 0,6). Kesimpulannya, Pengencer air kelapa muda viridis dapat mempertahankan kualitas semen cair kambing Boer selama 4 hari untuk motilitas dan 5 hari untuk viabilitas.Kata Kunci:pengencer, air kelapa, varietas viridisABSTRACTYoung viridis coconut water could be used as an alternative to liquid semen diluent for artificial insemination program in the area with limited facility for frozen semen production. This study evaluated the use of young coconut water as a diluent on liquid semen quality of Boer goat after cold storage. This study was carried out for 3 months at Sumber Sekar Laboratory, Faculty of Animal Husbandry, University of Brawijaya, Malang. The semen was collected from 3 Boer bucks aged at 3 to 5 years old. The semen collection was done once a week with the aid of artificial vagina. The diluents used were young Viridis coconut (5 to 7 months old) and tris aminomethane. The method used was an experiment in a randomized block design with 2 treatments and 10 replicates. The treatments used were T0: tris aminomethane + 10% egg yolk (control) and T1:  young Viridis coconut water + 10% egg yolk. Data were analyzed by analysis of variance using Genstat 18 software. The variables measured were sperm individual motility, viability, and abnormality. The results showed that the sperm individual motility in T1 survived up to 4 days (40.5± 24.3%), the best viability at 5 days (42.0±24.6%),  while the lowest abnormality at 7 days (1.31±0.6). It could be concluded that: 1. Tris aminomethane diluent has higher quality with the storage length up to 9 days, 2. Young Viridis coconut water diluent could preserve liquid semen quality of Boer goat up to 4 days for sperm motility and 5 days for sperm viability.Keywords: diluents, coconut water, viridis variety

scholarly journals Effect of dietary supplementation with omega-3 and -6 on fresh and frozen/thawed sperm quality of dogs

2017 ◽  
Vol 38 (5) ◽  
pp. 3069 ◽  
Author(s):  
Ana Carolina Rodrigues ◽  
Camila Montanari Ruiz ◽  
Carla Daniela Dan De Nardo ◽  
Gabriele Barros Mothé ◽  
Fabiano Martinez Rossi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Sperm Motility ◽  
Sperm Quality ◽  
Dietary Supplementation ◽  
Reproductive Efficiency ◽  
Omega 3 ◽  
Oral Supplementation ◽  
Animal Reproduction ◽  
Fresh Semen

For years, fatty acids have been recommended as a dietary supplement to improve canine hair. For animal reproduction, supplementation with omegas has been used to increase the reproductive efficiency and conception rate, but few studies have been conducted in dogs. The aim of this study was to evaluate the effects of daily dietary supplementation with omega-3 and -6 on the quality of fresh and frozen/thawed semen in canines. Semen was collected from seven dogs and evaluated for sperm motility, vigor, concentration, and morphology. The 17-week study included 119 ejaculates and was divided according to oral supplementation with omega-3 and -6: M1 (1st-5th week) or pre-supplementation; M2 (6th-9th week) and M3 (10th-13th week) or during supplementation; and M4 (14th-17th week) or post-supplementation. After analysis, the semen was frozen and then revaluated both immediately and 30 minutes (at 37° C) after thawing. Supplementation with omegas increased sperm motility, vigor, and concentration; however, supplementation had no influence on semen freezability. In addition, there was no improvement in sperm motility after supplementation when the thawed cells were maintained at 37° C for 30 minutes. We concluded that dietary supplementation with omega-3 and -6 for 4 to 8 weeks can improve the quality of fresh semen, although it has no effect on the freezability of canine semen.

scholarly journals PENGARUH PENGGUNAAN KONSENTRASI AIR KELAPA MUDA PADA PENGENCER NACL FISIOLOGIS TERHADAP MOTILITAS DAN MORTALITAS SPERMATOZOA IKAN TAWES ( Puntius javanicus)

2015 ◽  
Vol 2 (2) ◽  
Author(s):  
Farah Diana ◽  
Muhammad Rizal ◽  
Dewi Mariani
Keyword(s):  
Coconut Water ◽  
Motility Index ◽  
Sample Quality ◽  
Randomized Design ◽  
Research Show

This study aims to knowing influence using cocentration of coconut water in physiology of NaCl dilution toquality of spermatozoa of Puntius javanicus. This research was counducted at Meunasah Krueng Village, Beutong Subdistrict, Nagan Raya District from 18th – 20th May 2015. Thecompletely randomized design with 5 treatmentand 3 replication was used for this study. Sample was saved in refrigerator (3 days) with temperature 4oC and it is oberved sample quality and data was collected in daily. Result of the research show water coconut concentration (7%) or in treatment 4th (P4) giving result for best quality of spermatozoa, where motility index average of spermatozoa is up to 80,55%, viability value of spermatozoa is up to 65,88%. In addtion, coconut water in in physiology of NaCl dilution have been affecting to spermatozoa quality of Puntius javanicus. 

scholarly journals The Effect of Adding Different Levels of Curcumin and Its Nanoparticles to Extender on Post-Thaw Quality of Cryopreserved Rabbit Sperm

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1508 ◽  
Author(s):  
Sameh Abdelnour ◽  
Mahmoud Hassan ◽  
Amer Mohammed ◽  
Ahmad Alhimaidi ◽  
Naif Al-Gabri ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Membrane Integrity ◽  
Positive Influence ◽  
Control Group ◽  
Curcumin Nanoparticles ◽  
Total Antioxidant ◽  
Semen Extender ◽  
Apoptosis Process ◽  
Structural Levels

The cryopreservation process adversely affects sperm function and quality traits, causing some changes at biochemical and structural levels, due to mechanical, thermal, osmotic, and oxidative damage. Supplementation with curcumin nanoparticles could prevent and even revert this effect and could enhance the post/thawed sperm quality in the rabbit. The study amid to explore the effect of curcumin (CU) and curcumin nanoparticles (CUNPs) supplementation in semen extender on post/thawed rabbit sperm quality. Twelve fertile, healthy rabbit bucks were included, and the ejaculates were collected using artificial vaginas. Rabbit pooled semen was cryopreserved in tris-yolk fructose (TYF) extender without any supplement (control group) or extender supplemented with CU at levels of 0.5, 1 or 1.5 µg/mL (CU0.5, CU1.0, and CU1.5, respectively) or CUNPs at levels of 0.5, 1, 1.5 (CUNPs0.5, CUNPs1.0, and CUNPs1.5, respectively) and was packed in straws (0.25 mL) and stored in liquid nitrogen (−196 °C). Results revealed that CUNPs1.5 had a positive influence (p < 0.05) on post-thawing sperm progressive motility, viability, and membrane integrity as compared with the other groups. Percentages of dead sperm, abnormalities, early apoptotic, apoptotic, and necrotic sperm cells reduced (p < 0.05) in CUNPs1.5 as compared to other treatments. Using 1.5 µg/mL of CUNPs significantly improved total antioxidant capacity (TAC), GPx, while MDA and POC reduced (p < 0.05) in CU1.5 in comparison with other groups. SOD values were enhanced (p < 0.05) in CUNPs1.0 and CUNPs1.5 in relation with other treatments. Conclusively, the addition of curcumin and its nanoparticles to the extender can improve the post-thawed quality of rabbit sperm via redox signaling and reduce the apoptosis process.

scholarly journals Metformin Improves Quality of Post-Thaw Canine Semen

Animals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 287 ◽  
Author(s):  
Jérémy Grandhaye ◽  
Agnieszka Partyka ◽  
Zuzanna Ligocka ◽  
Agata Dudek ◽  
Wojciech Niżański ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Sperm Quality ◽  
Membrane Integrity ◽  
Fertilization Rate ◽  
Mitochondrial Activity ◽  
Assisted Reproductive Technique ◽  
Dna Damages ◽  
Semen Extender ◽  
Metabolic Activities

Sperm cryopreservation is an assisted reproductive technique routinely used in canine species for genetic conservation. However, during cryopreservation, the DNA damages are still elevated, limiting the fertilization rate. The present study was conducted to evaluate whether supplementation of canine semen extender with a molecule limiting the metabolic activities can improve the quality of frozen-thawed canine spermatozoa. We used metformin, known to limit the mitochondrial respiratory and limit the oxidative stress. Before and during the freezing procedure, metformin (50µM and 500µM) has been added to the extender. After thawing, sperm exposed to metformin conserved the same viability without alteration in the membrane integrity or acrosome reaction. Interestingly, 50µM metformin improved the sperm motility in comparison to the control, subsequently increasing mitochondrial activity and NAD+ content. In addition, the oxidative stress level was reduced in sperm treated with metformin improving the sperm quality as measured by a different molecular marker. In conclusion, we have shown that metformin is able to improve the quality of frozen-thawed dog semen when it is used during the cryopreservative procedure.

scholarly journals Kapasitasi Spermatozoa Sapi Peranakan Ongole dalam Berbagai Formulasi Pengencer Air Kelapa Selama Simpan Dingin

2019 ◽  
Vol 6 (1) ◽  
pp. 85
Author(s):  
Dedi Muhammad ◽  
Nurul Isnaini ◽  
Kuswati Kuswati ◽  
Aulia Puspita Anugra Yekti ◽  
Muchamad Luthfi ◽  
...  
Keyword(s):  
Experimental Design ◽  
Block Design ◽  
Coconut Water ◽  
Chilled Storage ◽  
Fructose 2

ABSTRAKPengencer dasar air kelapa merupakan solusi dari sulit dan mahalnya harga pengadaan bahan baku pembuatan pengencer. Tujuan dari penelitian ini adalah untuk mengetahui kapasitasi spermatozoa sapi Peranakan Ongole dalam berbagai formulasi pengencer air kelapa selama simpan dingin pada suhu 4-5oC. Metodologi yang digunakan adalah eksperimantal laboratorium menggunakan rancangan acak kelompok (RAK). Terdapat tiga perlakuan yaitu, P1 = Pengencer air kelapa + 20% kuning telur, P2 = Pengencer air kelapa + 20% kuning telur + 0,4% putih telur + fruktosa 1000 mg/l. P3 = Pengencer air kelapa + 20% kuning telur + 0,4% putih telur + fruktosa 2000 mg/l, dengan 10 ulangan. Hasil menunjukkan bahwa tidak terdapat perbedaan yang signifikan pada persentase spermatozoa belum kapasitasi, terkapasitasi dan telah rekasi akrosom antara perlakuan formulasi pengencer air kelapa yang berbeda (P1, P2, P3) selama peyimpana suhu 2-5oC. Pengencer berbasis air kelapa mampu mempertahankan kualitas akrosom diatas 50% penyimpanan hari ke lima.Kata kunci : air kelapa, kapasitasi, reaksi akrosom, semen cairABSTRACTCoconut water extender is the solution to the difficulty and high cots of semen diluents. The objective of this study were to examine the capacitation of Ongole Crossbreed sperm in various formulations of coconut water extender during storage in 4-5 oC. The study method used laboratory experimental. The experimental design was Randomized Completely Block Design and the data were analyzed by Analyze of Variance. There are three treatments in this study (P1 = Coconut Water + 20% of yolk, P2 = Coconut Water + 20% of yolk + 0,4% white egg + fructose 1 mg/ml, dan P3 = Coconut Water + 20% of yolk + 0,4% white egg + fructose 2 mg/ml) and ten replications of each treatment. There were not significant differences in percentage of sperm uncapacitation, sperm capacitaion and sperm acrosom reaction between various formulations of coconut water diluents during chilled storage at 4-5 oC. Coconut water diluents are able to maintain quality of sperm acrosom up to day 5th with values above 50%.Keywords: acrosom reaction, capacitation, coconut water, liquid semen

scholarly journals Pengaruh pengencer air kelapa tua yang berbeda varietas terhadap kualitas semen cair Kambing Boer pada penyimpanan 3-50C

2018 ◽  
Vol 28 (2) ◽  
pp. 112
Author(s):  
Achmad Fadhli Aziz ◽  
Muhammad Ade Salim ◽  
Nurul Isnaini ◽  
Aulia Puspita Anugra Yekti ◽  
Trinil Susilawati
Keyword(s):  
Artificial Insemination ◽  
Block Design ◽  
Egg Yolk ◽  
Coconut Water ◽  
Boer Goat ◽  
Randomized Block Design ◽  
Materials Used ◽  
Red Variety ◽  
Fresh Semen ◽  
Range Test

Artificial Insemination using liquid semen is technology reproduction can improve goat productivity without liquid nitrogen. The Purpose of this research was to determine differences of old coconut water varieties of red (<em>Cocos rubescens</em>) and green (<em>Cocos viridis</em>) as diluent Boer goat semen, based on motility, viability and spermabnormalities during storage at 3-5<sup>0</sup>C. The materials used for this research was Boer goat fresh semen as much as 3 heads were collected 2 times a week. Experimental Design. The treatment were devided into three Treatmen, there were: P0 (<em>Tris</em>+ 20% egg yolk); P1 (old coconut water varieties red + 20% egg yolk); P2 (old coconut water varieties green + 20% egg yolk). The data were analyzed by randomized block design, if the result are different or significantly different then continued to be tested with Duncan's multiple range test. The result showed that during storage until days 4, P0 has the best result on motility (40,5 <span style="text-decoration: underline;">+</span> 1,1 %), viability (45,18<span style="text-decoration: underline;">+ </span>1,56 %), and abnormality (1,89<span style="text-decoration: underline;">+</span> 0,59 %). While on the diluent using old coconut water green variety better then red variety. On day of 2 P1 and P2 have motility (61 <span style="text-decoration: underline;">+</span> 1,3%) and (51<span style="text-decoration: underline;">+</span> 7,5%); Viability (67,14<span style="text-decoration: underline;">+1</span>7,99%) and (55,37<span style="text-decoration: underline;">+</span>10,66%), abnormality (1,23 <span style="text-decoration: underline;">+ </span>1,02%) dan (1,70<span style="text-decoration: underline;">+</span> 0,99%).

scholarly journals Pengaruh Metode Thawing terhadap Kualitas Semen Beku Sapi Bali, Sapi Madura dan Sapi PO

2012 ◽  
Vol 12 (2) ◽  
pp. 14-19
Author(s):  
Muhammad Ade Salim ◽  
Trinil Susilawati ◽  
Sri Wahyuningsih
Keyword(s):  
Sperm Quality ◽  
Block Design ◽  
Bovine Semen ◽  
Bovine Sperm ◽  
Randomized Block Design ◽  
Frozen Semen ◽  
Temperature Water

Effect of thawing technique to quality frozen semen spermatozoa in bali, madura and PO cattleABSTRACT. The experiment was conducted from November 2011 to January 2012 in BBIB Singosari. The study aims was to determine the effect of thawing techniques on the quality frozen bovine sperm Bali, Madura and PO cattle. This material used 90 frozen straw of three cattle from the three breed. The method is an experiment with three treatments and 10 replications for frozen to thawing in temperature water is 5 0C of duration 60 seconds, 15 0C duration 30 seconds and 37 0C duration 15 seconds. Variables observed in this study were sperm quality are motility, viability and abnormality. Data in percentage transformed in to arcus sinus were analyzed using Completely Randomized Block Design (CRBD) with replication as blocks of 10x. The results showed the thawing at 37 0C duration 15 seconds to highly significant effect (P0,01) viability and motility and significant effect (P0.05). The abnormalities, no significant effect (P0,05). Breed cattle not significant effect to quality. It can be concluded that thawing techniques impact the motility and viability in frozen bovine semen spermatozoa Bali, Madura and PO cattle. Thawing using 37 0C temperature water with duration of 15 seconds is the best method to obtain the quality of spermatozoa post thawing frozen sperm.

scholarly journals CALLUS INDUCTION OF CACAO CLONE SULAWESI 1 ON VARIOUS CONCENTRATIONS OF 2,4 -D AND COCONUT WATER VIA IN VITRO CULTURE

2018 ◽  
Vol 4 (1) ◽  
pp. 35
Author(s):  
Asmila Asmila ◽  
Zainuddin Basri ◽  
Ramal Yusuf ◽  
Hawalina Hawalina
Keyword(s):  
In Vitro Culture ◽  
Culture Media ◽  
Callus Formation ◽  
Block Design ◽  
Coconut Water ◽  
Quality Of Products ◽  
Plantation Crops ◽  
Central Sulawesi

Cacao is one of important plantation crops grouped in the Sterculiaceae family.  Sulawesi is the main area of cacao production and has a number of superior clones, such as Sulawesi 1 and Sulawesi 2.  Based on data in 2012/2014 cacao production to consumption reached 174,000 tons, while in 2013/2014 was projected a deficit of 115,000 tonnes. Nonetheless, cacao agribusiness in Indonesia is still facing complex problems, among others gardener productivity is still low due to borer attacks cacao, the quality of products and the number is still low and still not optimal development of cacao products and providing superior amount of cacao seedlings. The primary problem of cacao production recently is low productivity.  The main cause of low cacao productivity in Central Sulawesi is the use of inferior clones.  To enhance cacao productivity, it is crucial to use cacao clones having high genetic potential via tissue culture or micropropagation techniques.  The aim of this experiment was to assess the effect of different concentrations of 2,4-D and coconut water on the growth of cacao callus via in vitro culture.  This experiment used Completely Randomozed Block Design in factorial patteren with treatments tested namely 2,4-D and coconut water concentrations.  The concentrations of 2,4-D tested including 1 ppm, 2 ppm and 3 ppm, whilst coconut water concentrations tested consisting of 10%, 15% and 20%, and therefore there were 3 x 3 = 9 treatment combinations.  Each treatment utilized 4 replications; and each unit combination used 5 explants (staminodia).  Results of this experiment indicated that the addition of 3 ppm 2,4-D and 10% coconut water had a highly significant effect on the callus color 4 weeks after culture.  The addition of 3 ppm 2,4-D in culture media showed a significant effect on callus color 4 weeks after culture, but had an insignificant effect on the callus formation, callus color 8 weeks after culture an callus texture.  Supplementation of 20% coconut water had a significant effect on callus texture 8 weeks after culture, whilst the addition of 10% coconut water showed a significant effect on callus color 4 weeks after culture.

Pengaruh Lama Thawing yang Berbeda pada Suhu 25 oC Terhadap Kualitas Semen Beku Sapi Ongole

2019 ◽  
Vol 6 (1) ◽  
pp. 119
Author(s):  
Enike Dwi Kusumawati ◽  
Syam Rahadi ◽  
Sugeng Santoso ◽  
Dyah Lestari Yulianti
Keyword(s):  
Sperm Quality ◽  
Animal Husbandry ◽  
Randomized Design ◽  
Frozen Semen ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
Bull Sperm

ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh lama thawing yang berbeda pada suhu 250C untuk mendapatkan kualitas spermatozoa semen beku sapi ongole yang optimal. Penelitian dilakukan di Laboratorium Fakultas Peternakan Universitas Kanjuruhan Malang. Metode Penelitian yang digunakan adalah metode percobaan dengan menggunakan Rancangan Acak Lengkap (RAL) Faktorial. Setiap perlakuan thawing diberikan ulangan sebanyak 10 sampel semen beku sapi ongole. Hasil penelitian menunjukkan bahwa perlakuan dengan lama thawing 7, 15, 30 detik pada suhu 25oC memberikan perbedaan pengaruh yang sangat nyata (p<0.01) pada motilitas, viabilitas dan abnormalitas spermatozoa semen beku sapi ongole. Motilitas tertinggi diperoleh pada perlakuan P3 dengan rata-rata sebesar 40,8%, viabilitas tertinggi diperoleh pada perlakuan P3 dengan rata-rata sebesar 82,39%, sedangkan abnormalitas terendah diperoleh pada perlakuan P3 dengan rata-rata sebesar 11,95%. Berdasarkan penelitian ini maka dapat disimpulkan bahwa thawing pada suhu 25oC dengan lama waktu 30 detik memberikan kualitas spermatozoa yang paling baik sehingga disarankan untuk melakukan thawing pada suhu 25oC dengan lama waktu 30 detik. Kata kunci: abnormalias, mortalitas, ongole, thawing, viabilitas ABSTRACT This study aims to determine the effect of different thawing time at 250C to get the sperm quality of frozen semen ongole bull. Research conducted at the Laboratory of Animal Husbandry Faculty Kanjuruhan University of Malang. The method of study by using Completely Randomized Design (CRD) factorial. Any treatment given repeated thawing of frozen samples of 10 times. The study show that treatment with time thawing 7 (P1), 15 (P2), 30 (P3) seconds at 25oC gives a very significant difference (P<0.01) on motility, viability and abnormalities of ongole Bull sperm. The highest motility and viability were obtained at P3 (40,8%) and (82,39%), while the lowest abnormalities obtained on P3 (11,95%). Based on this research it can be concluded that thawing at 25oC with 30 seconds to give the best quality sperm that is recommended for thawing. Keywords: abnormality, motility, ongole, thawing, viabilit

Sign in / Sign up

Export Citation Format

Share Document